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Protein

NAD(P)H-hydrate epimerase

Gene

Naxe

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the epimerization of the S- and R-forms of NAD(P)HX, a damaged form of NAD(P)H that is a result of enzymatic or heat-dependent hydration. This is a prerequisite for the S-specific NAD(P)H-hydrate dehydratase to allow the repair of both epimers of NAD(P)HX.UniRule annotation1 Publication

Catalytic activityi

(6R)-6-beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide = (6S)-6-beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide.
(6R)-6-beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide phosphate = (6S)-6-beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide phosphate.

Cofactori

K+Note: Binds 1 potassium ion per subunit.

Kineticsi

  1. KM=1.6 µM for (R)-NADHX1 Publication
  2. KM=0.33 µM for (R)-NADPHX1 Publication
  1. Vmax=0.26 µmol/min/mg enzyme toward (R)-NADHX1 Publication
  2. Vmax=1.2 µmol/min/mg enzyme toward (R)-NADPHX1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi114PotassiumUniRule annotation1
Metal bindingi179PotassiumUniRule annotation1
Binding sitei212NAD(P)HXUniRule annotation1
Metal bindingi215PotassiumUniRule annotation1

GO - Molecular functioni

GO - Biological processi

  • protein homotetramerization Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Isomerase

Keywords - Ligandi

Metal-binding, NAD, NADP, Nucleotide-binding, Potassium

Enzyme and pathway databases

BRENDAi5.1.99.6. 3474.
ReactomeiR-MMU-197264. Nicotinamide salvaging.

Names & Taxonomyi

Protein namesi
Recommended name:
NAD(P)H-hydrate epimeraseUniRule annotation (EC:5.1.99.6)
Alternative name(s):
Apolipoprotein A-I-binding proteinUniRule annotation
Short name:
AI-BPUniRule annotation
NAD(P)HX epimeraseBy similarity
Gene namesi
Name:NaxeBy similarity
Synonyms:Aibp, Apoa1bp
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 3

Organism-specific databases

MGIiMGI:2180167. Apoa1bp.

Subcellular locationi

GO - Cellular componenti

  • cell body Source: MGI
  • cilium Source: MGI
  • cytoplasm Source: MGI
  • cytosol Source: MGI
  • extracellular exosome Source: MGI
  • extracellular region Source: MGI
  • extracellular space Source: MGI
  • intracellular membrane-bounded organelle Source: MGI
  • mitochondrion Source: MGI
  • nucleoplasm Source: MGI
  • nucleus Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 53MitochondrionUniRule annotationAdd BLAST53
ChainiPRO_000029242354 – 282NAD(P)H-hydrate epimeraseAdd BLAST229

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei43Phosphoserine; by PKACombined sources1 Publication1
Modified residuei138N6-succinyllysineCombined sources1

Post-translational modificationi

Undergoes physiological phosphorylation during sperm capacitation, downstream to PKA activation.UniRule annotation1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ8K4Z3.
MaxQBiQ8K4Z3.
PaxDbiQ8K4Z3.
PeptideAtlasiQ8K4Z3.
PRIDEiQ8K4Z3.
TopDownProteomicsiQ8K4Z3.

2D gel databases

REPRODUCTION-2DPAGEQ8K4Z3.

PTM databases

iPTMnetiQ8K4Z3.
PhosphoSitePlusiQ8K4Z3.
SwissPalmiQ8K4Z3.

Expressioni

Tissue specificityi

Detected in testis and sperm (at protein level). Expressed at high levels in heart, liver, kidney, and testis.1 Publication

Gene expression databases

BgeeiENSMUSG00000028070.
CleanExiMM_APOA1BP.
GenevisibleiQ8K4Z3. MM.

Interactioni

Subunit structurei

Interacts with APOA1 and APOA2 (By similarity). Homodimer.By similarity1 Publication

GO - Molecular functioni

  • protein homodimerization activity Source: MGI

Protein-protein interaction databases

DIPiDIP-59952N.
IntActiQ8K4Z3. 3 interactors.
MINTiMINT-1869651.
STRINGi10090.ENSMUSP00000029708.

Structurei

Secondary structure

1282
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi56 – 67Combined sources12
Turni68 – 70Combined sources3
Helixi74 – 92Combined sources19
Helixi95 – 97Combined sources3
Beta strandi98 – 102Combined sources5
Beta strandi104 – 109Combined sources6
Helixi113 – 127Combined sources15
Beta strandi131 – 135Combined sources5
Helixi143 – 154Combined sources12
Beta strandi159 – 162Combined sources4
Helixi167 – 173Combined sources7
Beta strandi175 – 181Combined sources7
Helixi194 – 202Combined sources9
Beta strandi208 – 213Combined sources6
Turni219 – 221Combined sources3
Beta strandi230 – 237Combined sources8
Helixi240 – 244Combined sources5
Beta strandi247 – 253Combined sources7
Helixi259 – 264Combined sources6
Beta strandi277 – 280Combined sources4

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2DG2X-ray2.45A/B/C/D/E/F25-282[»]
2O8NX-ray2.00A25-282[»]
3RNOX-ray2.50A25-282[»]
3RO7X-ray2.50A25-282[»]
3ROEX-ray2.11A/B/C/D/E/F25-282[»]
3ROGX-ray2.05A25-282[»]
3ROXX-ray2.40A25-282[»]
3ROZX-ray2.80A25-282[»]
ProteinModelPortaliQ8K4Z3.
SMRiQ8K4Z3.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ8K4Z3.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini59 – 269YjeF N-terminalUniRule annotationAdd BLAST211

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni113 – 117NAD(P)HXUniRule annotation5
Regioni183 – 189NAD(P)HXUniRule annotation7

Sequence similaritiesi

Belongs to the NnrE/AIBP family.UniRule annotation
Contains 1 YjeF N-terminal domain.UniRule annotation

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG2585. Eukaryota.
COG0062. LUCA.
GeneTreeiENSGT00390000007227.
HOGENOMiHOG000174236.
HOVERGENiHBG058276.
InParanoidiQ8K4Z3.
KOiK17759.
OMAiHLGMFGY.
OrthoDBiEOG091G0J79.
PhylomeDBiQ8K4Z3.
TreeFamiTF300197.

Family and domain databases

Gene3Di3.40.50.10260. 1 hit.
HAMAPiMF_01966. NADHX_epimerase. 1 hit.
InterProiIPR004443. YjeF_N_dom.
IPR032976. YJEFN_prot_eukaryotes.
[Graphical view]
PANTHERiPTHR13232. PTHR13232. 1 hit.
PfamiPF03853. YjeF_N. 1 hit.
[Graphical view]
SUPFAMiSSF64153. SSF64153. 1 hit.
TIGRFAMsiTIGR00197. yjeF_nterm. 1 hit.
PROSITEiPS51385. YJEF_N. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q8K4Z3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSGLRTLLGL GLLVAGSRLP RVISQQSVCR ARPIWWGTQR RGSETMAGAA
60 70 80 90 100
VKYLSQEEAQ AVDQELFNEY QFSVDQLMEL AGLSCATAIA KAYPPTSMSK
110 120 130 140 150
SPPTVLVICG PGNNGGDGLV CARHLKLFGY QPTIYYPKRP NKPLFTGLVT
160 170 180 190 200
QCQKMDIPFL GEMPPEPMMV DELYELVVDA IFGFSFKGDV REPFHSILSV
210 220 230 240 250
LSGLTVPIAS IDIPSGWDVE KGNPSGIQPD LLISLTAPKK SATHFTGRYH
260 270 280
YLGGRFVPPA LEKKYQLNLP SYPDTECVYR LQ
Length:282
Mass (Da):30,973
Last modified:October 1, 2002 - v1
Checksum:iDFD95155755D8D96
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ344092 mRNA. Translation: CAC86966.1.
AY566271 mRNA. Translation: AAT70236.1.
AK159846 mRNA. Translation: BAE35424.1.
BC058362 mRNA. Translation: AAH58362.1.
CCDSiCCDS17464.1.
RefSeqiNP_659146.1. NM_144897.3.
UniGeneiMm.205996.

Genome annotation databases

EnsembliENSMUST00000029708; ENSMUSP00000029708; ENSMUSG00000028070.
GeneIDi246703.
KEGGimmu:246703.
UCSCiuc008ptu.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ344092 mRNA. Translation: CAC86966.1.
AY566271 mRNA. Translation: AAT70236.1.
AK159846 mRNA. Translation: BAE35424.1.
BC058362 mRNA. Translation: AAH58362.1.
CCDSiCCDS17464.1.
RefSeqiNP_659146.1. NM_144897.3.
UniGeneiMm.205996.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2DG2X-ray2.45A/B/C/D/E/F25-282[»]
2O8NX-ray2.00A25-282[»]
3RNOX-ray2.50A25-282[»]
3RO7X-ray2.50A25-282[»]
3ROEX-ray2.11A/B/C/D/E/F25-282[»]
3ROGX-ray2.05A25-282[»]
3ROXX-ray2.40A25-282[»]
3ROZX-ray2.80A25-282[»]
ProteinModelPortaliQ8K4Z3.
SMRiQ8K4Z3.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-59952N.
IntActiQ8K4Z3. 3 interactors.
MINTiMINT-1869651.
STRINGi10090.ENSMUSP00000029708.

PTM databases

iPTMnetiQ8K4Z3.
PhosphoSitePlusiQ8K4Z3.
SwissPalmiQ8K4Z3.

2D gel databases

REPRODUCTION-2DPAGEQ8K4Z3.

Proteomic databases

EPDiQ8K4Z3.
MaxQBiQ8K4Z3.
PaxDbiQ8K4Z3.
PeptideAtlasiQ8K4Z3.
PRIDEiQ8K4Z3.
TopDownProteomicsiQ8K4Z3.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000029708; ENSMUSP00000029708; ENSMUSG00000028070.
GeneIDi246703.
KEGGimmu:246703.
UCSCiuc008ptu.1. mouse.

Organism-specific databases

CTDi246703.
MGIiMGI:2180167. Apoa1bp.

Phylogenomic databases

eggNOGiKOG2585. Eukaryota.
COG0062. LUCA.
GeneTreeiENSGT00390000007227.
HOGENOMiHOG000174236.
HOVERGENiHBG058276.
InParanoidiQ8K4Z3.
KOiK17759.
OMAiHLGMFGY.
OrthoDBiEOG091G0J79.
PhylomeDBiQ8K4Z3.
TreeFamiTF300197.

Enzyme and pathway databases

BRENDAi5.1.99.6. 3474.
ReactomeiR-MMU-197264. Nicotinamide salvaging.

Miscellaneous databases

ChiTaRSiApoa1bp. mouse.
EvolutionaryTraceiQ8K4Z3.
PROiQ8K4Z3.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000028070.
CleanExiMM_APOA1BP.
GenevisibleiQ8K4Z3. MM.

Family and domain databases

Gene3Di3.40.50.10260. 1 hit.
HAMAPiMF_01966. NADHX_epimerase. 1 hit.
InterProiIPR004443. YjeF_N_dom.
IPR032976. YJEFN_prot_eukaryotes.
[Graphical view]
PANTHERiPTHR13232. PTHR13232. 1 hit.
PfamiPF03853. YjeF_N. 1 hit.
[Graphical view]
SUPFAMiSSF64153. SSF64153. 1 hit.
TIGRFAMsiTIGR00197. yjeF_nterm. 1 hit.
PROSITEiPS51385. YJEF_N. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiNNRE_MOUSE
AccessioniPrimary (citable) accession number: Q8K4Z3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 26, 2007
Last sequence update: October 1, 2002
Last modified: November 2, 2016
This is version 112 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.