Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Fibronectin type III domain-containing protein 5

Gene

Fndc5

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Irisin: mediates beneficial effects of muscular exercise. Induces browning of white adipose tissue by stimulating UCP1 expression, at least in part, via the nuclear receptor PPARA.1 Publication

GO - Biological processi

  • positive regulation of brown fat cell differentiation Source: UniProtKB
  • response to muscle activity Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Hormone

Names & Taxonomyi

Protein namesi
Recommended name:
Fibronectin type III domain-containing protein 5
Alternative name(s):
Fibronectin type III repeat-containing protein 2
Peroxisomal protein
Short name:
PeP
Cleaved into the following chain:
Gene namesi
Name:Fndc5
Synonyms:Frcp2, Pep
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 4

Organism-specific databases

MGIiMGI:1917614. Fndc5.

Subcellular locationi

Irisin :
  • Secreted

  • Note: Detected in the blood of individuals subjected to endurance exercise.

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini29 – 149ExtracellularSequence analysisAdd BLAST121
Transmembranei150 – 170HelicalSequence analysisAdd BLAST21
Topological domaini171 – 209CytoplasmicSequence analysisAdd BLAST39

GO - Cellular componenti

  • endoplasmic reticulum Source: UniProtKB
  • extracellular region Source: UniProtKB
  • integral component of membrane Source: UniProtKB-KW
  • peroxisomal membrane Source: UniProtKB-SubCell
  • plasma membrane Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane, Peroxisome, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 28Sequence analysisAdd BLAST28
ChainiPRO_000032897229 – 209Fibronectin type III domain-containing protein 5Add BLAST181
ChainiPRO_000041585829 – 140IrisinAdd BLAST112

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi36N-linked (GlcNAc...)1 Publication1
Glycosylationi81N-linked (GlcNAc...)1 Publication1

Post-translational modificationi

The extracellular domain is cleaved and released from the cell membrane.
N-Glycosylated.1 Publication

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiQ8K4Z2.
PRIDEiQ8K4Z2.

PTM databases

iPTMnetiQ8K4Z2.
PhosphoSitePlusiQ8K4Z2.

Expressioni

Tissue specificityi

In adult, it is highly expressed in skeletal muscle, heart and brain.2 Publications

Developmental stagei

During embryonic development, it is detected almost exclusively in the skeletal muscle, and at lower level in brain. In skeletal muscle, it is induced after myoblast differentiation, when myotube formation is promoted.1 Publication

Inductioni

Up-regulated by muscular exercise. This effect can be mediated, at least partly, by PPARGC1A.1 Publication

Gene expression databases

BgeeiENSMUSG00000001334.
GenevisibleiQ8K4Z2. MM.

Interactioni

Protein-protein interaction databases

IntActiQ8K4Z2. 1 interactor.
MINTiMINT-217983.
STRINGi10090.ENSMUSP00000099660.

Structurei

3D structure databases

ProteinModelPortaliQ8K4Z2.
SMRiQ8K4Z2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini33 – 124Fibronectin type-IIIPROSITE-ProRule annotationAdd BLAST92

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi207 – 209Microbody targeting signalSequence analysis3

Sequence similaritiesi

Contains 1 fibronectin type-III domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IESX. Eukaryota.
ENOG410YC2F. LUCA.
GeneTreeiENSGT00390000004923.
HOGENOMiHOG000082536.
HOVERGENiHBG067308.
InParanoidiQ8K4Z2.
OMAiNEPNNSK.
OrthoDBiEOG091G0HLN.
PhylomeDBiQ8K4Z2.
TreeFamiTF325415.

Family and domain databases

CDDicd00063. FN3. 1 hit.
Gene3Di2.60.40.10. 1 hit.
InterProiIPR003961. FN3_dom.
IPR032073. FNDC5_C.
IPR013783. Ig-like_fold.
[Graphical view]
PfamiPF16066. DUF4808. 1 hit.
PF00041. fn3. 1 hit.
[Graphical view]
SMARTiSM00060. FN3. 1 hit.
[Graphical view]
SUPFAMiSSF49265. SSF49265. 1 hit.
PROSITEiPS50853. FN3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q8K4Z2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPPGPCAWPP RAALRLWLGC VCFALVQADS PSAPVNVTVR HLKANSAVVS
60 70 80 90 100
WDVLEDEVVI GFAISQQKKD VRMLRFIQEV NTTTRSCALW DLEEDTEYIV
110 120 130 140 150
HVQAISIQGQ SPASEPVLFK TPREAEKMAS KNKDEVTMKE MGRNQQLRTG
160 170 180 190 200
EVLIIVVVLF MWAGVIALFC RQYDIIKDNE PNNNKEKTKS ASETSTPEHQ

GGGLLRSKI
Length:209
Mass (Da):23,321
Last modified:October 1, 2002 - v1
Checksum:i86A33DE8E4616507
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti13 – 14AL → VV in BAB23815 (PubMed:16141072).Curated2
Sequence conflicti64I → L in BAB23815 (PubMed:16141072).Curated1
Sequence conflicti124E → A in BAB23815 (PubMed:16141072).Curated1
Sequence conflicti130S → P in BAB23815 (PubMed:16141072).Curated1
Sequence conflicti158V → C in BAB23815 (PubMed:16141072).Curated1
Sequence conflicti171R → A in BAB23815 (PubMed:16141072).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ401376 mRNA. Translation: CAC82194.1.
AK005096 mRNA. Translation: BAB23815.1.
AL606977 Genomic DNA. Translation: CAM19716.1.
BC109183 mRNA. Translation: AAI09184.1.
BC109184 mRNA. Translation: AAI09185.1.
CCDSiCCDS18683.1.
RefSeqiNP_081678.1. NM_027402.4.
UniGeneiMm.44075.

Genome annotation databases

EnsembliENSMUST00000102600; ENSMUSP00000099660; ENSMUSG00000001334.
GeneIDi384061.
KEGGimmu:384061.
UCSCiuc008uwe.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ401376 mRNA. Translation: CAC82194.1.
AK005096 mRNA. Translation: BAB23815.1.
AL606977 Genomic DNA. Translation: CAM19716.1.
BC109183 mRNA. Translation: AAI09184.1.
BC109184 mRNA. Translation: AAI09185.1.
CCDSiCCDS18683.1.
RefSeqiNP_081678.1. NM_027402.4.
UniGeneiMm.44075.

3D structure databases

ProteinModelPortaliQ8K4Z2.
SMRiQ8K4Z2.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ8K4Z2. 1 interactor.
MINTiMINT-217983.
STRINGi10090.ENSMUSP00000099660.

PTM databases

iPTMnetiQ8K4Z2.
PhosphoSitePlusiQ8K4Z2.

Proteomic databases

PaxDbiQ8K4Z2.
PRIDEiQ8K4Z2.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000102600; ENSMUSP00000099660; ENSMUSG00000001334.
GeneIDi384061.
KEGGimmu:384061.
UCSCiuc008uwe.1. mouse.

Organism-specific databases

CTDi252995.
MGIiMGI:1917614. Fndc5.

Phylogenomic databases

eggNOGiENOG410IESX. Eukaryota.
ENOG410YC2F. LUCA.
GeneTreeiENSGT00390000004923.
HOGENOMiHOG000082536.
HOVERGENiHBG067308.
InParanoidiQ8K4Z2.
OMAiNEPNNSK.
OrthoDBiEOG091G0HLN.
PhylomeDBiQ8K4Z2.
TreeFamiTF325415.

Miscellaneous databases

ChiTaRSiFndc5. mouse.
PROiQ8K4Z2.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000001334.
GenevisibleiQ8K4Z2. MM.

Family and domain databases

CDDicd00063. FN3. 1 hit.
Gene3Di2.60.40.10. 1 hit.
InterProiIPR003961. FN3_dom.
IPR032073. FNDC5_C.
IPR013783. Ig-like_fold.
[Graphical view]
PfamiPF16066. DUF4808. 1 hit.
PF00041. fn3. 1 hit.
[Graphical view]
SMARTiSM00060. FN3. 1 hit.
[Graphical view]
SUPFAMiSSF49265. SSF49265. 1 hit.
PROSITEiPS50853. FN3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiFNDC5_MOUSE
AccessioniPrimary (citable) accession number: Q8K4Z2
Secondary accession number(s): Q9DB97
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 8, 2008
Last sequence update: October 1, 2002
Last modified: November 30, 2016
This is version 106 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.