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Protein

Fibronectin type III domain-containing protein 5

Gene

Fndc5

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Irisin: mediates beneficial effects of muscular exercise. Induces browning of white adipose tissue by stimulating UCP1 expression, at least in part, via the nuclear receptor PPARA.1 Publication

GO - Biological processi

  • positive regulation of brown fat cell differentiation Source: UniProtKB
  • response to muscle activity Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Hormone

Names & Taxonomyi

Protein namesi
Recommended name:
Fibronectin type III domain-containing protein 5
Alternative name(s):
Fibronectin type III repeat-containing protein 2
Peroxisomal protein
Short name:
PeP
Cleaved into the following chain:
Gene namesi
Name:Fndc5
Synonyms:Frcp2, Pep
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 4

Organism-specific databases

MGIiMGI:1917614. Fndc5.

Subcellular locationi

Irisin :
  • Secreted

  • Note: Detected in the blood of individuals subjected to endurance exercise.

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini29 – 149121ExtracellularSequence analysisAdd
BLAST
Transmembranei150 – 17021HelicalSequence analysisAdd
BLAST
Topological domaini171 – 20939CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

  • endoplasmic reticulum Source: UniProtKB
  • extracellular region Source: UniProtKB
  • integral component of membrane Source: UniProtKB-KW
  • peroxisomal membrane Source: UniProtKB-SubCell
  • plasma membrane Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane, Peroxisome, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2828Sequence analysisAdd
BLAST
Chaini29 – 209181Fibronectin type III domain-containing protein 5PRO_0000328972Add
BLAST
Chaini29 – 140112IrisinPRO_0000415858Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi36 – 361N-linked (GlcNAc...)1 Publication
Glycosylationi81 – 811N-linked (GlcNAc...)1 Publication

Post-translational modificationi

The extracellular domain is cleaved and released from the cell membrane.
N-Glycosylated.1 Publication

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiQ8K4Z2.
PRIDEiQ8K4Z2.

PTM databases

iPTMnetiQ8K4Z2.
PhosphoSiteiQ8K4Z2.

Expressioni

Tissue specificityi

In adult, it is highly expressed in skeletal muscle, heart and brain.2 Publications

Developmental stagei

During embryonic development, it is detected almost exclusively in the skeletal muscle, and at lower level in brain. In skeletal muscle, it is induced after myoblast differentiation, when myotube formation is promoted.1 Publication

Inductioni

Up-regulated by muscular exercise. This effect can be mediated, at least partly, by PPARGC1A.1 Publication

Gene expression databases

BgeeiQ8K4Z2.
GenevisibleiQ8K4Z2. MM.

Interactioni

Protein-protein interaction databases

IntActiQ8K4Z2. 1 interaction.
MINTiMINT-217983.
STRINGi10090.ENSMUSP00000099660.

Structurei

3D structure databases

ProteinModelPortaliQ8K4Z2.
SMRiQ8K4Z2. Positions 30-127.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini33 – 12492Fibronectin type-IIIPROSITE-ProRule annotationAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi207 – 2093Microbody targeting signalSequence analysis

Sequence similaritiesi

Contains 1 fibronectin type-III domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IESX. Eukaryota.
ENOG410YC2F. LUCA.
GeneTreeiENSGT00390000004923.
HOGENOMiHOG000082536.
HOVERGENiHBG067308.
InParanoidiQ8K4Z2.
OMAiNEPNNSK.
OrthoDBiEOG7S2206.
PhylomeDBiQ8K4Z2.
TreeFamiTF325415.

Family and domain databases

Gene3Di2.60.40.10. 1 hit.
InterProiIPR003961. FN3_dom.
IPR032073. FNDC5_C.
IPR013783. Ig-like_fold.
[Graphical view]
PfamiPF16066. DUF4808. 1 hit.
PF00041. fn3. 1 hit.
[Graphical view]
SMARTiSM00060. FN3. 1 hit.
[Graphical view]
SUPFAMiSSF49265. SSF49265. 1 hit.
PROSITEiPS50853. FN3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q8K4Z2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPPGPCAWPP RAALRLWLGC VCFALVQADS PSAPVNVTVR HLKANSAVVS
60 70 80 90 100
WDVLEDEVVI GFAISQQKKD VRMLRFIQEV NTTTRSCALW DLEEDTEYIV
110 120 130 140 150
HVQAISIQGQ SPASEPVLFK TPREAEKMAS KNKDEVTMKE MGRNQQLRTG
160 170 180 190 200
EVLIIVVVLF MWAGVIALFC RQYDIIKDNE PNNNKEKTKS ASETSTPEHQ

GGGLLRSKI
Length:209
Mass (Da):23,321
Last modified:October 1, 2002 - v1
Checksum:i86A33DE8E4616507
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti13 – 142AL → VV in BAB23815 (PubMed:16141072).Curated
Sequence conflicti64 – 641I → L in BAB23815 (PubMed:16141072).Curated
Sequence conflicti124 – 1241E → A in BAB23815 (PubMed:16141072).Curated
Sequence conflicti130 – 1301S → P in BAB23815 (PubMed:16141072).Curated
Sequence conflicti158 – 1581V → C in BAB23815 (PubMed:16141072).Curated
Sequence conflicti171 – 1711R → A in BAB23815 (PubMed:16141072).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ401376 mRNA. Translation: CAC82194.1.
AK005096 mRNA. Translation: BAB23815.1.
AL606977 Genomic DNA. Translation: CAM19716.1.
BC109183 mRNA. Translation: AAI09184.1.
BC109184 mRNA. Translation: AAI09185.1.
CCDSiCCDS18683.1.
RefSeqiNP_081678.1. NM_027402.3.
UniGeneiMm.44075.

Genome annotation databases

EnsembliENSMUST00000102600; ENSMUSP00000099660; ENSMUSG00000001334.
GeneIDi384061.
KEGGimmu:384061.
UCSCiuc008uwe.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ401376 mRNA. Translation: CAC82194.1.
AK005096 mRNA. Translation: BAB23815.1.
AL606977 Genomic DNA. Translation: CAM19716.1.
BC109183 mRNA. Translation: AAI09184.1.
BC109184 mRNA. Translation: AAI09185.1.
CCDSiCCDS18683.1.
RefSeqiNP_081678.1. NM_027402.3.
UniGeneiMm.44075.

3D structure databases

ProteinModelPortaliQ8K4Z2.
SMRiQ8K4Z2. Positions 30-127.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ8K4Z2. 1 interaction.
MINTiMINT-217983.
STRINGi10090.ENSMUSP00000099660.

PTM databases

iPTMnetiQ8K4Z2.
PhosphoSiteiQ8K4Z2.

Proteomic databases

PaxDbiQ8K4Z2.
PRIDEiQ8K4Z2.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000102600; ENSMUSP00000099660; ENSMUSG00000001334.
GeneIDi384061.
KEGGimmu:384061.
UCSCiuc008uwe.1. mouse.

Organism-specific databases

CTDi252995.
MGIiMGI:1917614. Fndc5.

Phylogenomic databases

eggNOGiENOG410IESX. Eukaryota.
ENOG410YC2F. LUCA.
GeneTreeiENSGT00390000004923.
HOGENOMiHOG000082536.
HOVERGENiHBG067308.
InParanoidiQ8K4Z2.
OMAiNEPNNSK.
OrthoDBiEOG7S2206.
PhylomeDBiQ8K4Z2.
TreeFamiTF325415.

Miscellaneous databases

ChiTaRSiFndc5. mouse.
PROiQ8K4Z2.
SOURCEiSearch...

Gene expression databases

BgeeiQ8K4Z2.
GenevisibleiQ8K4Z2. MM.

Family and domain databases

Gene3Di2.60.40.10. 1 hit.
InterProiIPR003961. FN3_dom.
IPR032073. FNDC5_C.
IPR013783. Ig-like_fold.
[Graphical view]
PfamiPF16066. DUF4808. 1 hit.
PF00041. fn3. 1 hit.
[Graphical view]
SMARTiSM00060. FN3. 1 hit.
[Graphical view]
SUPFAMiSSF49265. SSF49265. 1 hit.
PROSITEiPS50853. FN3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Mouse PeP: a novel peroxisomal protein linked to myoblast differentiation and development."
    Ferrer-Martinez A., Ruiz-Lozano P., Chien K.R.
    Dev. Dyn. 224:154-167(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], SUBCELLULAR LOCATION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
    Strain: BALB/cJ.
    Tissue: Heart.
  2. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Cerebellum.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  5. Cited for: PROTEIN SEQUENCE OF 133-140, FUNCTION, INDUCTION, GLYCOSYLATION, SUBCELLULAR LOCATION, IDENTIFICATION BY MASS SPECTROMETRY.
  6. "Frcp1 and Frcp2, two novel fibronectin type III repeat containing genes."
    Teufel A., Malik N., Mukhopadhyay M., Westphal H.
    Gene 297:79-83(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY.
  7. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brain.

Entry informationi

Entry nameiFNDC5_MOUSE
AccessioniPrimary (citable) accession number: Q8K4Z2
Secondary accession number(s): Q9DB97
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 8, 2008
Last sequence update: October 1, 2002
Last modified: June 8, 2016
This is version 103 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.