ID   CANT1_RAT               Reviewed;         403 AA.
AC   Q8K4Y7; Q4V8N9;
DT   10-MAY-2005, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-2002, sequence version 1.
DT   16-JUN-2009, entry version 48.
DE   RecName: Full=Soluble calcium-activated nucleotidase 1;
DE            Short=SCAN-1;
DE            EC=3.6.1.6;
DE   AltName: Full=Apyrase homolog;
GN   Name=Cant1; Synonyms=Srapy;
OS   Rattus norvegicus (Rat).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Sciurognathi;
OC   Muroidea; Muridae; Murinae; Rattus.
OX   NCBI_TaxID=10116;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, COFACTOR, SUBCELLULAR LOCATION,
RP   AND TISSUE SPECIFICITY.
RC   STRAIN=Sprague-Dawley; TISSUE=Brain;
RX   MEDLINE=22241955; PubMed=12167635; DOI=10.1074/jbc.M201656200;
RA   Failer B.U., Braun N., Zimmermann H.;
RT   "Cloning, expression, and functional characterization of a Ca2+-
RT   dependent endoplasmic reticulum nucleoside diphosphatase.";
RL   J. Biol. Chem. 277:36978-36986(2002).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Placenta;
RX   PubMed=15489334; DOI=10.1101/gr.2596504;
RG   The MGC Project Team;
RT   "The status, quality, and expansion of the NIH full-length cDNA
RT   project: the Mammalian Gene Collection (MGC).";
RL   Genome Res. 14:2121-2127(2004).
CC   -!- FUNCTION: Calcium-dependent nucleotidase with a preference for
CC       UDP. The order of activity with different substrates is UDP > GDP
CC       > IDP >> UTP > CDP = GTP = ITP. Has very low activity towards ADP
CC       and even lower activity towards ATP. Does not hydrolyze AMP and
CC       GMP.
CC   -!- CATALYTIC ACTIVITY: A nucleoside diphosphate + H(2)O = a
CC       nucleotide + phosphate.
CC   -!- COFACTOR: Calcium.
CC   -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC       Kinetic parameters:
CC         KM=216 uM for UDP;
CC   -!- SUBUNIT: Monomer (By similarity).
CC   -!- SUBCELLULAR LOCATION: Endoplasmic reticulum membrane; Single-pass
CC       type II membrane protein. Golgi apparatus, Golgi stack membrane;
CC       Single-pass type II membrane protein. Note=Processed form:
CC       Secreted.
CC   -!- TISSUE SPECIFICITY: Detected in intestine, thymus, heart, lung,
CC       spleen, kidney, liver, testis, skeletal muscle and brain.
CC   -!- SIMILARITY: Belongs to the apyrase family.
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DR   EMBL; AJ312207; CAC85467.1; -; mRNA.
DR   EMBL; BC097279; AAH97279.1; -; mRNA.
DR   IPI; IPI00203735; -.
DR   RefSeq; NP_653355.1; -.
DR   UniGene; Rn.7241; -.
DR   SMR; Q8K4Y7; 89-403.
DR   PRIDE; Q8K4Y7; -.
DR   Ensembl; ENSRNOG00000003239; Rattus norvegicus.
DR   GeneID; 246272; -.
DR   KEGG; rno:246272; -.
DR   RGD; 628743; Cant1.
DR   HOVERGEN; Q8K4Y7; -.
DR   BRENDA; 3.6.1.6; 248.
DR   NextBio; 623641; -.
DR   ArrayExpress; Q8K4Y7; -.
DR   GermOnline; ENSRNOG00000003239; Rattus norvegicus.
DR   GO; GO:0005789; C:endoplasmic reticulum membrane; IDA:RGD.
DR   GO; GO:0032580; C:Golgi cisterna membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0016021; C:integral to membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005509; F:calcium ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0017110; F:nucleoside-diphosphatase activity; IEA:EC.
DR   InterPro; IPR009283; Apyrase.
DR   PANTHER; PTHR13023; Apyrase; 1.
DR   Pfam; PF06079; Apyrase; 1.
PE   1: Evidence at protein level;
KW   Calcium; Endoplasmic reticulum; Glycoprotein; Golgi apparatus;
KW   Hydrolase; Membrane; Metal-binding; Signal-anchor; Transmembrane.
FT   CHAIN         1    403       Soluble calcium-activated nucleotidase 1.
FT                                /FTId=PRO_0000209927.
FT   TOPO_DOM      1     44       Cytoplasmic (Potential).
FT   TRANSMEM     45     61       Signal-anchor for type II membrane
FT                                protein (Potential).
FT   TOPO_DOM     62    403       Lumenal (Potential).
FT   COMPBIAS    236    239       Poly-Thr.
FT   METAL       170    170       Calcium (By similarity).
FT   METAL       217    217       Calcium (By similarity).
FT   METAL       286    286       Calcium (By similarity).
FT   METAL       347    347       Calcium (By similarity).
FT   METAL       398    398       Calcium (By similarity).
FT   CARBOHYD     90     90       N-linked (GlcNAc...) (Potential).
SQ   SEQUENCE   403 AA;  45659 MW;  34340AF7917334F1 CRC64;
     MPIQPFDQRE WNEPMHSLRI SVGGLPVLAS MTKATDPRFR PRWRVILTSF VGAALLWLLY
     SHHQTPVSGR PPIHNAHNWR LRQERISQYN DTYPLSPPQR TPGGIRYRIA VIADLDTGSK
     AQEENTWFSY LKKGYLTLSD SGDRVSVEWD KDRGVLESHL AEKGRGMELS DLIVFNGKLY
     SVDDRTGVIY QIEGTKAVPW VILSDGDGAV EKGFKAEWLA VKDEHLYVGG LGKEWTTTTG
     EVVNENPEWV KVVGHRGSVE HENWVSSYNA LRAAAGIQPP GYLIHESACW SDTLQRWFFL
     PRRASHERYS EREDERKGSN LLLSAAQDFR DISVRQVGAL VPTHGFSSFK FIPNTDDQII
     VALKSEEDNG RIATYVMAFT LDGRFLLPET KIGSVKYEGI EFI
//