Reviewed, UniProtKB/Swiss-Prot Q8K4Y7 (CANT1_RAT)

Names and origin

Protein names

Recommended name:
    Soluble calcium-activated nucleotidase 1
      Short name=SCAN-1
    EC=3.6.1.6
Alternative name(s):
    Apyrase homolog

Gene names

Name:	Cant1
Synonyms:	Srapy

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	403 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Calcium-dependent nucleotidase with a preference for UDP. The order of activity with different substrates is UDP > GDP > IDP >> UTP > CDP = GTP = ITP. Has very low activity towards ADP and even lower activity towards ATP. Does not hydrolyze AMP and GMP. Ref.1
Catalytic activity	A nucleoside diphosphate + H2O = a nucleotide + phosphate.
Cofactor	Calcium. Ref.1
Subunit structure	Monomer By similarity.
Subcellular location	Endoplasmic reticulum membrane; Single-pass type II membrane protein. Golgi apparatus › Golgi stack membrane; Single-pass type II membrane protein. Note: Processed form: Secreted. Ref.1
Tissue specificity	Detected in intestine, thymus, heart, lung, spleen, kidney, liver, testis, skeletal muscle and brain. Ref.1
Sequence similarities	Belongs to the apyrase family.
biophysicochemical properties	Kinetic parameters: KM=216 µM for UDP

Ontologies

Keywords
Cellular component	Endoplasmic reticulum Golgi apparatus Membrane
Domain	Signal-anchor Transmembrane
Ligand	Calcium Metal-binding
Molecular function	Hydrolase
PTM	Glycoprotein
Gene Ontology (GO)
Cellular component	Golgi cisterna membrane Inferred from electronic annotation. Source: UniProtKB-SubCell endoplasmic reticulum membrane Ref.1 Inferred from direct assay. Source: RGD integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	calcium ion binding Inferred from electronic annotation. Source: UniProtKB-KW nucleoside-diphosphatase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 403	403	Soluble calcium-activated nucleotidase 1		PRO_0000209927
Regions
	Topological domain	1 – 44	44	Cytoplasmic Potential
	Transmembrane	45 – 61	17	Signal-anchor for type II membrane protein Potential
	Topological domain	62 – 403	342	Lumenal Potential
	Compositional bias	236 – 239	4	Poly-Thr
Sites
	Metal binding	170	1	Calcium By similarity
	Metal binding	217	1	Calcium By similarity
	Metal binding	286	1	Calcium By similarity
	Metal binding	347	1	Calcium By similarity
	Metal binding	398	1	Calcium By similarity
Amino acid modifications
	Glycosylation	90	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	Q8K4Y7-1 [UniParc]. Last modified October 1, 2002. Version 1. Checksum: 34340AF7917334F1	FASTA	403	45,659
	10 20 30 40 50 60 MPIQPFDQRE WNEPMHSLRI SVGGLPVLAS MTKATDPRFR PRWRVILTSF VGAALLWLLY 70 80 90 100 110 120 SHHQTPVSGR PPIHNAHNWR LRQERISQYN DTYPLSPPQR TPGGIRYRIA VIADLDTGSK 130 140 150 160 170 180 AQEENTWFSY LKKGYLTLSD SGDRVSVEWD KDRGVLESHL AEKGRGMELS DLIVFNGKLY 190 200 210 220 230 240 SVDDRTGVIY QIEGTKAVPW VILSDGDGAV EKGFKAEWLA VKDEHLYVGG LGKEWTTTTG 250 260 270 280 290 300 EVVNENPEWV KVVGHRGSVE HENWVSSYNA LRAAAGIQPP GYLIHESACW SDTLQRWFFL 310 320 330 340 350 360 PRRASHERYS EREDERKGSN LLLSAAQDFR DISVRQVGAL VPTHGFSSFK FIPNTDDQII 370 380 390 400 VALKSEEDNG RIATYVMAFT LDGRFLLPET KIGSVKYEGI EFI

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning, expression, and functional characterization of a Ca2+-dependent endoplasmic reticulum nucleoside diphosphatase." Failer B.U., Braun N., Zimmermann H. J. Biol. Chem. 277:36978-36986(2002) [PubMed: 12167635] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, COFACTOR, SUBCELLULAR LOCATION, TISSUE SPECIFICITY. Strain: Sprague-Dawley. Tissue: Brain.
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Placenta.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AJ312207 mRNA. Translation: CAC85467.1. BC097279 mRNA. Translation: AAH97279.1.
IPI	IPI00203735.
RefSeq	NP_653355.1.
UniGene	Rn.7241
3D structure databases
SMR	Q8K4Y7. Positions 89-403.
ModBase	Search...
Proteomic databases
PRIDE	Q8K4Y7.
Genome annotation databases
Ensembl	ENSRNOG00000003239. Rattus norvegicus. [Contig view]
GeneID	246272.
KEGG	rno:246272.
Organism-specific databases
RGD	628743. Cant1.
Phylogenomic databases
HOVERGEN	Q8K4Y7.
Enzyme and pathway databases
BRENDA	3.6.1.6. 248.
Gene expression databases
ArrayExpress	Q8K4Y7.
GermOnline	ENSRNOG00000003239. Rattus norvegicus.
Family and domain databases
InterPro	IPR009283. Apyrase. [Graphical view]
PANTHER	PTHR13023. Apyrase. 1 hit.
Pfam	PF06079. Apyrase. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	623641.

Entry information

Entry name

CANT1_RAT

Accession

Primary (citable) accession number: Q8K4Y7
Secondary accession number(s): Q4V8N9

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 10, 2005
Last sequence update:	October 1, 2002
Last modified:	June 16, 2009
This is version 48 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)