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Protein

Soluble calcium-activated nucleotidase 1

Gene

Cant1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Calcium-dependent nucleotidase with a preference for UDP. The order of activity with different substrates is UDP > GDP > IDP >> UTP > CDP = GTP = ITP. Has very low activity towards ADP and even lower activity towards ATP. Does not hydrolyze AMP and GMP (PubMed:12167635). Involved in proteoglycan synthesis (By similarity).By similarity1 Publication

Catalytic activityi

A nucleoside diphosphate + H2O = a nucleoside phosphate + phosphate.1 Publication

Cofactori

Ca2+1 Publication

Kineticsi

  1. KM=216 µM for UDP1 Publication

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Sitei162Important for dimer formationBy similarity1
    Metal bindingi170Calcium; via carbonyl oxygenBy similarity1
    Metal bindingi171CalciumBy similarity1
    Sitei202Important for dimer formationBy similarity1
    Sitei204Important for dimer formationBy similarity1
    Metal bindingi217Calcium; via carbonyl oxygenBy similarity1
    Sitei258Important for dimer formationBy similarity1
    Metal bindingi286Calcium; via carbonyl oxygenBy similarity1
    Metal bindingi347Calcium; via carbonyl oxygenBy similarity1
    Metal bindingi398Calcium; via carbonyl oxygenBy similarity1

    GO - Molecular functioni

    GO - Biological processi

    Complete GO annotation...

    Keywords - Molecular functioni

    Hydrolase

    Keywords - Ligandi

    Calcium, Metal-binding

    Enzyme and pathway databases

    BRENDAi3.6.1.6. 5301.
    ReactomeiR-RNO-6798695. Neutrophil degranulation.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Soluble calcium-activated nucleotidase 1 (EC:3.6.1.61 Publication)
    Short name:
    SCAN-1
    Alternative name(s):
    Apyrase homolog
    Gene namesi
    Name:Cant1
    Synonyms:Srapy
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    Proteomesi
    • UP000002494 Componenti: Chromosome 10

    Organism-specific databases

    RGDi628743. Cant1.

    Subcellular locationi

    Topology

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Topological domaini1 – 44CytoplasmicSequence analysisAdd BLAST44
    Transmembranei45 – 61Helical; Signal-anchor for type II membrane proteinSequence analysisAdd BLAST17
    Topological domaini62 – 403LumenalSequence analysisAdd BLAST342

    GO - Cellular componenti

    Complete GO annotation...

    Keywords - Cellular componenti

    Endoplasmic reticulum, Golgi apparatus, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00002099271 – 403Soluble calcium-activated nucleotidase 1Add BLAST403

    Amino acid modifications

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Glycosylationi90N-linked (GlcNAc...)Sequence analysis1

    Keywords - PTMi

    Glycoprotein

    Proteomic databases

    PaxDbiQ8K4Y7.
    PRIDEiQ8K4Y7.

    PTM databases

    PhosphoSitePlusiQ8K4Y7.

    Expressioni

    Tissue specificityi

    Detected in intestine, thymus, heart, lung, spleen, kidney, liver, testis, skeletal muscle and brain.1 Publication

    Gene expression databases

    BgeeiENSRNOG00000003239.
    GenevisibleiQ8K4Y7. RN.

    Interactioni

    Subunit structurei

    Monomer. Homodimer; dimerization is Ca2+-dependent.By similarity

    Protein-protein interaction databases

    MINTiMINT-4579530.
    STRINGi10116.ENSRNOP00000059931.

    Structurei

    3D structure databases

    ProteinModelPortaliQ8K4Y7.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Compositional bias

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Compositional biasi236 – 239Poly-Thr4

    Sequence similaritiesi

    Belongs to the apyrase family.Curated

    Keywords - Domaini

    Signal-anchor, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiKOG4494. Eukaryota.
    ENOG410XS4T. LUCA.
    GeneTreeiENSGT00390000012872.
    HOGENOMiHOG000008129.
    HOVERGENiHBG059824.
    InParanoidiQ8K4Y7.
    KOiK12304.
    OMAiVVPTHGF.
    OrthoDBiEOG091G0DL5.

    Family and domain databases

    InterProiIPR009283. Apyrase.
    [Graphical view]
    PANTHERiPTHR13023. PTHR13023. 1 hit.
    PfamiPF06079. Apyrase. 1 hit.
    [Graphical view]
    SUPFAMiSSF101887. SSF101887. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q8K4Y7-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MPIQPFDQRE WNEPMHSLRI SVGGLPVLAS MTKATDPRFR PRWRVILTSF
    60 70 80 90 100
    VGAALLWLLY SHHQTPVSGR PPIHNAHNWR LRQERISQYN DTYPLSPPQR
    110 120 130 140 150
    TPGGIRYRIA VIADLDTGSK AQEENTWFSY LKKGYLTLSD SGDRVSVEWD
    160 170 180 190 200
    KDRGVLESHL AEKGRGMELS DLIVFNGKLY SVDDRTGVIY QIEGTKAVPW
    210 220 230 240 250
    VILSDGDGAV EKGFKAEWLA VKDEHLYVGG LGKEWTTTTG EVVNENPEWV
    260 270 280 290 300
    KVVGHRGSVE HENWVSSYNA LRAAAGIQPP GYLIHESACW SDTLQRWFFL
    310 320 330 340 350
    PRRASHERYS EREDERKGSN LLLSAAQDFR DISVRQVGAL VPTHGFSSFK
    360 370 380 390 400
    FIPNTDDQII VALKSEEDNG RIATYVMAFT LDGRFLLPET KIGSVKYEGI

    EFI
    Length:403
    Mass (Da):45,659
    Last modified:October 1, 2002 - v1
    Checksum:i34340AF7917334F1
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AJ312207 mRNA. Translation: CAC85467.1.
    BC097279 mRNA. Translation: AAH97279.1.
    RefSeqiNP_653355.1. NM_144754.2.
    XP_006247851.1. XM_006247789.3.
    XP_006247852.1. XM_006247790.1.
    UniGeneiRn.7241.

    Genome annotation databases

    EnsembliENSRNOT00000066303; ENSRNOP00000059931; ENSRNOG00000003239.
    GeneIDi246272.
    KEGGirno:246272.
    UCSCiRGD:628743. rat.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AJ312207 mRNA. Translation: CAC85467.1.
    BC097279 mRNA. Translation: AAH97279.1.
    RefSeqiNP_653355.1. NM_144754.2.
    XP_006247851.1. XM_006247789.3.
    XP_006247852.1. XM_006247790.1.
    UniGeneiRn.7241.

    3D structure databases

    ProteinModelPortaliQ8K4Y7.
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    MINTiMINT-4579530.
    STRINGi10116.ENSRNOP00000059931.

    PTM databases

    PhosphoSitePlusiQ8K4Y7.

    Proteomic databases

    PaxDbiQ8K4Y7.
    PRIDEiQ8K4Y7.

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsembliENSRNOT00000066303; ENSRNOP00000059931; ENSRNOG00000003239.
    GeneIDi246272.
    KEGGirno:246272.
    UCSCiRGD:628743. rat.

    Organism-specific databases

    CTDi124583.
    RGDi628743. Cant1.

    Phylogenomic databases

    eggNOGiKOG4494. Eukaryota.
    ENOG410XS4T. LUCA.
    GeneTreeiENSGT00390000012872.
    HOGENOMiHOG000008129.
    HOVERGENiHBG059824.
    InParanoidiQ8K4Y7.
    KOiK12304.
    OMAiVVPTHGF.
    OrthoDBiEOG091G0DL5.

    Enzyme and pathway databases

    BRENDAi3.6.1.6. 5301.
    ReactomeiR-RNO-6798695. Neutrophil degranulation.

    Miscellaneous databases

    PROiQ8K4Y7.

    Gene expression databases

    BgeeiENSRNOG00000003239.
    GenevisibleiQ8K4Y7. RN.

    Family and domain databases

    InterProiIPR009283. Apyrase.
    [Graphical view]
    PANTHERiPTHR13023. PTHR13023. 1 hit.
    PfamiPF06079. Apyrase. 1 hit.
    [Graphical view]
    SUPFAMiSSF101887. SSF101887. 1 hit.
    ProtoNetiSearch...

    Entry informationi

    Entry nameiCANT1_RAT
    AccessioniPrimary (citable) accession number: Q8K4Y7
    Secondary accession number(s): Q4V8N9
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 10, 2005
    Last sequence update: October 1, 2002
    Last modified: November 30, 2016
    This is version 97 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.