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Protein

Leucine-rich glioma-inactivated protein 1

Gene

Lgi1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Plays a role in suppressing the production of MMP1/3 through the phosphatidylinositol 3-kinase/ERK pathway (By similarity). Regulates voltage-gated potassium channels assembled from KCNA1, KCNA4 and KCNAB1. It slows down channel inactivation by precluding channel closure mediated by the KCNAB1 subunit. Ligand for ADAM22 that positively regulates synaptic transmission mediated by AMPA-type glutamate receptors.By similarity2 Publications

GO - Biological processi

  • axon guidance Source: Ensembl
  • positive regulation of cell growth Source: Ensembl
  • positive regulation of synaptic transmission Source: UniProtKB
  • protein homooligomerization Source: UniProtKB
Complete GO annotation...

Enzyme and pathway databases

ReactomeiR-RNO-5682910. LGI-ADAM interactions.

Names & Taxonomyi

Protein namesi
Recommended name:
Leucine-rich glioma-inactivated protein 1
Gene namesi
Name:Lgi1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 1

Organism-specific databases

RGDi628742. Lgi1.

Subcellular locationi

GO - Cellular componenti

  • cell junction Source: UniProtKB-KW
  • extracellular space Source: UniProtKB
  • synapse Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Secreted, Synapse

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi383 – 3831E → A: Fails to bind to ADAM22. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 3434Sequence analysisAdd
BLAST
Chaini35 – 557523Leucine-rich glioma-inactivated protein 1PRO_0000017707Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi192 – 1921N-linked (GlcNAc...)Sequence analysis
Glycosylationi277 – 2771N-linked (GlcNAc...)Sequence analysis
Glycosylationi422 – 4221N-linked (GlcNAc...)Sequence analysis

Post-translational modificationi

Glycosylated.By similarity

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiQ8K4Y5.
PRIDEiQ8K4Y5.

PTM databases

iPTMnetiQ8K4Y5.
PhosphoSiteiQ8K4Y5.
SwissPalmiQ8K4Y5.

Expressioni

Tissue specificityi

Expressed in brain. High levels found in hippocampus, thalamic nuclei, neocortex, and molecular and granule cell layers of the cerebellum.1 Publication

Gene expression databases

GenevisibleiQ8K4Y5. RN.

Interactioni

Subunit structurei

Oligomer. Interacts with KCNA1 within a complex containing KCNA1, KCNA4 and KCNAB1. Can bind to ADAM11 and ADAM23 (By similarity). Part of a complex containing ADAM22, DLG4/PSD95 and CACNG2 (stargazin).By similarity2 Publications

Protein-protein interaction databases

BioGridi251653. 1 interaction.
STRINGi10116.ENSRNOP00000020411.

Structurei

3D structure databases

ProteinModelPortaliQ8K4Y5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini35 – 7238LRRNTAdd
BLAST
Repeati92 – 11322LRR 1Add
BLAST
Repeati116 – 13722LRR 2Add
BLAST
Repeati140 – 16122LRR 3Add
BLAST
Domaini173 – 22351LRRCTAdd
BLAST
Repeati224 – 26744EAR 1Add
BLAST
Repeati270 – 31344EAR 2Add
BLAST
Repeati316 – 36449EAR 3Add
BLAST
Repeati365 – 41551EAR 4Add
BLAST
Repeati418 – 46245EAR 5Add
BLAST
Repeati463 – 50644EAR 6Add
BLAST
Repeati509 – 55244EAR 7Add
BLAST

Sequence similaritiesi

Contains 7 EAR repeats.PROSITE-ProRule annotation
Contains 3 LRR (leucine-rich) repeats.Curated
Contains 1 LRRCT domain.Curated
Contains 1 LRRNT domain.Curated

Keywords - Domaini

Leucine-rich repeat, Repeat, Signal

Phylogenomic databases

eggNOGiENOG410IIXR. Eukaryota.
ENOG410XSZV. LUCA.
GeneTreeiENSGT00840000129728.
HOGENOMiHOG000286015.
HOVERGENiHBG036744.
InParanoidiQ8K4Y5.
OMAiAFNCDCK.
OrthoDBiEOG78SQHH.
PhylomeDBiQ8K4Y5.
TreeFamiTF333155.

Family and domain databases

Gene3Di3.80.10.10. 1 hit.
InterProiIPR000483. Cys-rich_flank_reg_C.
IPR009039. EAR.
IPR005492. EPTP.
IPR032675. L_dom-like.
IPR001611. Leu-rich_rpt.
IPR003591. Leu-rich_rpt_typical-subtyp.
[Graphical view]
PfamiPF03736. EPTP. 7 hits.
PF13855. LRR_8. 1 hit.
[Graphical view]
SMARTiSM00369. LRR_TYP. 3 hits.
SM00082. LRRCT. 1 hit.
[Graphical view]
SUPFAMiSSF52058. SSF52058. 1 hit.
PROSITEiPS50912. EAR. 7 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q8K4Y5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MESESIRRMG NACIPLKRIA YFLCLFSVVL LTEGKKPAKP KCPAVCTCSK
60 70 80 90 100
DNALCENARS IPRTVPPDVI SLSFVRSGFT EISEGSFLFT PSLQLLLFTS
110 120 130 140 150
NSFDVISDDA FIGLPHLEYL FIENNNIKSI SRHTFRGLKS LIHLSLANNN
160 170 180 190 200
LQTLPKDIFK GLDSLTNVDL RGNSFNCDCK LKWLVEWLGH TNATVEDIYC
210 220 230 240 250
EGPPEYKKRK INSLSPKDFD CIITEFAKSQ DLPYQSLSID TFSYLNDEYV
260 270 280 290 300
VIAQPFTGKC IFLEWDHVEK TFRNYDNITG TSTVVCKPIV IDTQLYVIVA
310 320 330 340 350
QLFGGSHIYK RDGFANKFIK IQDIEVLKIR KPNDIETFKI EDNWYFVVAD
360 370 380 390 400
SSKAGFTTIY KWNGNGFYSH QSLHAWYRDT DVEYLEIARP PLTLRTPHLI
410 420 430 440 450
LSSSSQRPVI YQWSKATQLF INQTDIPNME DVYAVKHFSV KGDVYICLTR
460 470 480 490 500
FIGDSKVMKW GGSSFQDIQR MPSRGSMVFQ PLQINNYQYA ILGSDYSFTQ
510 520 530 540 550
VYNWDAEKAK FVKFQELNVQ APRSFTHVSI NKRNFLFASS FKGNTQIYKH

VIVDLSA
Length:557
Mass (Da):63,728
Last modified:October 1, 2002 - v1
Checksum:i2296F36B6C91C32B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ487517 mRNA. Translation: CAD31785.1.
BC089222 mRNA. Translation: AAH89222.1.
RefSeqiNP_665712.1. NM_145769.3.
UniGeneiRn.83615.

Genome annotation databases

EnsembliENSRNOT00000020411; ENSRNOP00000020411; ENSRNOG00000014758.
GeneIDi252892.
KEGGirno:252892.
UCSCiRGD:628742. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ487517 mRNA. Translation: CAD31785.1.
BC089222 mRNA. Translation: AAH89222.1.
RefSeqiNP_665712.1. NM_145769.3.
UniGeneiRn.83615.

3D structure databases

ProteinModelPortaliQ8K4Y5.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi251653. 1 interaction.
STRINGi10116.ENSRNOP00000020411.

PTM databases

iPTMnetiQ8K4Y5.
PhosphoSiteiQ8K4Y5.
SwissPalmiQ8K4Y5.

Proteomic databases

PaxDbiQ8K4Y5.
PRIDEiQ8K4Y5.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000020411; ENSRNOP00000020411; ENSRNOG00000014758.
GeneIDi252892.
KEGGirno:252892.
UCSCiRGD:628742. rat.

Organism-specific databases

CTDi9211.
RGDi628742. Lgi1.

Phylogenomic databases

eggNOGiENOG410IIXR. Eukaryota.
ENOG410XSZV. LUCA.
GeneTreeiENSGT00840000129728.
HOGENOMiHOG000286015.
HOVERGENiHBG036744.
InParanoidiQ8K4Y5.
OMAiAFNCDCK.
OrthoDBiEOG78SQHH.
PhylomeDBiQ8K4Y5.
TreeFamiTF333155.

Enzyme and pathway databases

ReactomeiR-RNO-5682910. LGI-ADAM interactions.

Miscellaneous databases

PROiQ8K4Y5.

Gene expression databases

GenevisibleiQ8K4Y5. RN.

Family and domain databases

Gene3Di3.80.10.10. 1 hit.
InterProiIPR000483. Cys-rich_flank_reg_C.
IPR009039. EAR.
IPR005492. EPTP.
IPR032675. L_dom-like.
IPR001611. Leu-rich_rpt.
IPR003591. Leu-rich_rpt_typical-subtyp.
[Graphical view]
PfamiPF03736. EPTP. 7 hits.
PF13855. LRR_8. 1 hit.
[Graphical view]
SMARTiSM00369. LRR_TYP. 3 hits.
SM00082. LRRCT. 1 hit.
[Graphical view]
SUPFAMiSSF52058. SSF52058. 1 hit.
PROSITEiPS50912. EAR. 7 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The novel EPTP repeat defines a superfamily of proteins implicated in epileptic disorders."
    Staub E., Perez-Tur J., Siebert R., Nobile C., Moschonas N.K., Deloukas P., Hinzmann B.
    Trends Biochem. Sci. 27:441-444(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: Wistar.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Brain.
  3. "The epilepsy-linked Lgi1 protein assembles into presynaptic Kv1 channels and inhibits inactivation by Kvbeta1."
    Schulte U., Thumfart J.-O., Kloecker N., Sailer C.A., Bildl W., Biniossek M., Dehn D., Deller T., Eble S., Abbass K., Wangler T., Knaus H.-G., Fakler B.
    Neuron 49:697-706(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH KCNA1; KCNA4 AND KCNAB1, TISSUE SPECIFICITY, SUBCELLULAR LOCATION.
  4. "Epilepsy-related ligand/receptor complex LGI1 and ADAM22 regulate synaptic transmission."
    Fukata Y., Adesnik H., Iwanaga T., Bredt D.S., Nicoll R.A., Fukata M.
    Science 313:1792-1795(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION, SUBUNIT, INTERACTION WITH ADAM22; DLG4/PSD95 AND CACNG2, MUTAGENESIS OF GLU-383.

Entry informationi

Entry nameiLGI1_RAT
AccessioniPrimary (citable) accession number: Q8K4Y5
Secondary accession number(s): Q5FWS7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 3, 2003
Last sequence update: October 1, 2002
Last modified: June 8, 2016
This is version 100 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.