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Protein

Sorting nexin-27

Gene

Snx27

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Involved in the retrograde transport from endosome to plasma membrane, a trafficking pathway that promotes the recycling of internalized transmembrane proteins. Following internalization, endocytosed transmembrane proteins are delivered to early endosomes and recycled to the plasma membrane instead of being degraded in lysosomes. SNX27 specifically binds and directs sorting of a subset of transmembrane proteins containing a PDZ-binding motif at the C-terminus: following interaction with target transmembrane proteins, associates with the retromer complex, preventing entry into the lysosomal pathway, and promotes retromer-tubule based plasma membrane recycling. SNX27 also binds with the WASH complex. Interacts with membranes containing phosphatidylinositol-3-phosphate (PtdIns(3P)). May participate in establishment of natural killer cell polarity. Recruits CYTIP to early endosomes.2 Publications

GO - Molecular functioni

  • phosphatidylinositol-3-phosphate binding Source: UniProtKB

GO - Biological processi

  • cellular response to nerve growth factor stimulus Source: RGD
  • endosomal transport Source: UniProtKB
  • endosome to plasma membrane protein transport Source: RGD
  • establishment of protein localization to plasma membrane Source: RGD
  • intracellular protein transport Source: UniProtKB
  • negative regulation of inward rectifier potassium channel activity Source: RGD
  • nervous system development Source: RGD
  • positive regulation of AMPA glutamate receptor clustering Source: RGD
  • response to methamphetamine hydrochloride Source: RGD
  • retrograde transport, endosome to plasma membrane Source: UniProtKB
  • signal transduction Source: InterPro

Keywordsi

Biological processProtein transport, Transport
LigandLipid-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Sorting nexin-27
Alternative name(s):
MAP-responsive gene protein
Methamphetamine-responsive transcript 1 protein
PDZ-protein Mrt1
Gene namesi
Name:Snx27
Synonyms:Mrt1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi628705 Snx27

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Endosome, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi51Y → L: Abolishes interaction with KCNJ9. 1 Publication1
Mutagenesisi220K → A: Abolishes phosphatidylinositol-3-phosphate-binding and impairs subcellular location. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003153581 – 539Sorting nexin-27Add BLAST539

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei49PhosphoserineCombined sources1
Modified residuei60PhosphoserineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ8K4V4
PRIDEiQ8K4V4

PTM databases

iPTMnetiQ8K4V4
PhosphoSitePlusiQ8K4V4

Expressioni

Tissue specificityi

Isoform 1 is predominantly expressed in the testis, whereas isoform 2 is predominant in various brain regions, including, neocortex, paleocortex, striatum, hippocampus, cerebellum and brain stem. Expressed in cells of hematopoietic origin.4 Publications

Inductioni

Isoform 2 is up-regulated by methamphetamine and cocaine in the neocortex, but not by pentobarbital nor by D1 antagonist.1 Publication

Interactioni

Subunit structurei

Core component of the SNX27-retromer, a multiprotein complex composed of SNX27, the WASH complex and the retromer complex. Interacts (via PDZ domain) with a number of target transmembrane proteins (via PDZ-binding motif): ABCC4, ADRB2, ARHGEF7, GRIA1, GRIA2, GRIN1, GRIN2A GRIN2C, KCNJ6, KCNJ9 and SLC2A1/GLUT1. Interacts (via the FERM-like regions) with the WASH complex. Interacts with SNX1. Interacts with CYTIP. Interacts with DGKZ. Interacts with MCC.2 Publications

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000067026

Structurei

Secondary structure

1539
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi40 – 45Combined sources6
Beta strandi53 – 62Combined sources10
Beta strandi66 – 68Combined sources3
Beta strandi71 – 74Combined sources4
Beta strandi78 – 82Combined sources5
Helixi87 – 90Combined sources4
Beta strandi98 – 102Combined sources5
Helixi112 – 119Combined sources8
Beta strandi121 – 131Combined sources11

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3QDOX-ray1.88A39-133[»]
3QE1X-ray1.68A39-133[»]
3QGLX-ray3.31A/B/C/D/E39-133[»]
4P2AX-ray2.70B39-133[»]
4Z8JX-ray0.95A39-133[»]
5ELQX-ray1.10A/B39-133[»]
5EM9X-ray1.60A39-133[»]
5EMAX-ray1.32A39-133[»]
5EMBX-ray0.85A39-133[»]
ProteinModelPortaliQ8K4V4
SMRiQ8K4V4
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ8K4V4

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini41 – 134PDZPROSITE-ProRule annotationAdd BLAST94
Domaini159 – 267PXPROSITE-ProRule annotationAdd BLAST109
Domaini271 – 360Ras-associatingPROSITE-ProRule annotationAdd BLAST90

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni271 – 360FERM-like region F1By similarityAdd BLAST90
Regioni371 – 419FERM-like region F2By similarityAdd BLAST49
Regioni423 – 523FERM-like region F3By similarityAdd BLAST101

Domaini

The PDZ domain mediates binding to a subset of proteins containing a PDZ-binding motif at the C-terminus: the specificity for PDZ-binding motif is provided by the 2 residues located upstream of the canonical PDZ-binding motif (PubMed:17828261, PubMed:21422294). The PDZ domain also mediates binding to the retromer complex via direct interaction with VPS26 (VPS26A or VPS26B).2 Publications
The PX domain mediates binding to phosphatidylinositol 3-phosphate (PtdIns(3P)) and localization to early endosome membranes.1 Publication

Phylogenomic databases

eggNOGiENOG410IMRD Eukaryota
ENOG410XPAD LUCA
HOGENOMiHOG000016641
HOVERGENiHBG059206
InParanoidiQ8K4V4
KOiK17936
PhylomeDBiQ8K4V4

Family and domain databases

CDDicd13338 FERM-like_C_SNX27, 1 hit
cd06886 PX_SNX27, 1 hit
cd01777 SNX27_RA, 1 hit
Gene3Di3.30.1520.10, 1 hit
InterProiView protein in InterPro
IPR001478 PDZ
IPR036034 PDZ_sf
IPR001683 Phox
IPR036871 PX_dom_sf
IPR000159 RA_dom
IPR037831 SNX17/27/31
IPR028667 SNX27
IPR037827 SNX27_FERM-like_dom
IPR037833 SNX27_PX
IPR037835 SNX27_RA
IPR029071 Ubiquitin-like_domsf
PANTHERiPTHR12431 PTHR12431, 1 hit
PTHR12431:SF17 PTHR12431:SF17, 1 hit
PfamiView protein in Pfam
PF00595 PDZ, 1 hit
PF00787 PX, 1 hit
PF00788 RA, 1 hit
SMARTiView protein in SMART
SM00228 PDZ, 1 hit
SM00312 PX, 1 hit
SUPFAMiSSF50156 SSF50156, 1 hit
SSF54236 SSF54236, 1 hit
SSF64268 SSF64268, 1 hit
PROSITEiView protein in PROSITE
PS50106 PDZ, 1 hit
PS50195 PX, 1 hit
PS50200 RA, 1 hit

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8K4V4-1) [UniParc]FASTAAdd to basket
Also known as: Mrt1alpha, Mrt1a, SNX27a

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MADEDGEGIH PSTPHRNGGG GGGSGLHCAG NGGGGGGGPR VVRIVKSESG
60 70 80 90 100
YGFNVRGQVS EGGQLRSING ELYAPLQHVS AVLPGGAADR AGVRKGDRIL
110 120 130 140 150
EVNGVNVEGA THKQVVDLIR AGEKELILTV LSVPPHEADN LDPSDDSLGQ
160 170 180 190 200
SFYDYTEKQA VPISVPTYKH VEQNGEKFVV YNVYMAGRQL CSKRYREFAI
210 220 230 240 250
LHQNLKREFA NFTFPRLPGK WPFSLSEQQL DARRRGLEEY LEKVCSIRVI
260 270 280 290 300
GESDIMQEFL SESDENYNGV SDVELRVALP DGATVTVRVK KNSTTDQVYQ
310 320 330 340 350
AIAAKVGMDS TTVNYFALFE VINHSFVRKL APNEFPHKLY VQNYTSAVPG
360 370 380 390 400
TCLTIRKWLF TTEEEVLLND NDLAVTYFFH QAVDDVKKGY IKAEEKSYQL
410 420 430 440 450
QKLYEQRKMV MYLNMLRTCE GYNEIIFPHC ACDSRRKGHV ITAISITHFK
460 470 480 490 500
LHACTEEGQL ENQVIAFEWD EMQRWDTDEE GMAFCFEYAR GEKKPRWVKI
510 520 530
FTPYFNYMHE CFERVFCELK WRKENIFQMA RSQQRDVAT
Length:539
Mass (Da):61,015
Last modified:January 15, 2008 - v2
Checksum:iB5EAAD425FCD19F4
GO
Isoform 2 (identifier: Q8K4V4-2) [UniParc]FASTAAdd to basket
Also known as: Mrt1beta, Mrt1b, SNX27b

The sequence of this isoform differs from the canonical sequence as follows:
     525-539: NIFQMARSQQRDVAT → EY

Show »
Length:526
Mass (Da):59,560
Checksum:i5C9651E2788C5F09
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti236G → E in BAC10332 (PubMed:12740601).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_030541525 – 539NIFQM…RDVAT → EY in isoform 2. 1 PublicationAdd BLAST15

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB010245 mRNA Translation: BAC10332.1
AB051816 mRNA Translation: BAC10333.1
RefSeqiNP_001103621.1, NM_001110151.1
NP_690060.2, NM_152847.2
UniGeneiRn.7581

Genome annotation databases

GeneIDi260323
KEGGirno:260323
UCSCiRGD:628705 rat [Q8K4V4-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiSNX27_RAT
AccessioniPrimary (citable) accession number: Q8K4V4
Secondary accession number(s): Q8K4T6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: January 15, 2008
Last modified: May 23, 2018
This is version 95 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references

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