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Protein

Sorting nexin-27

Gene

Snx27

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in the retrograde transport from endosome to plasma membrane, a trafficking pathway that promotes the recycling of internalized transmembrane proteins. Following internalization, endocytosed transmembrane proteins are delivered to early endosomes and recycled to the plasma membrane instead of being degraded in lysosomes. SNX27 specifically binds and directs sorting of a subset of transmembrane proteins containing a PDZ-binding motif at the C-terminus: following interaction with target transmembrane proteins, associates with the retromer complex, preventing entry into the lysosomal pathway, and promotes retromer-tubule based plasma membrane recycling. SNX27 also binds with the WASH complex. Interacts with membranes containing phosphatidylinositol-3-phosphate (PtdIns(3P)). May participate in establishment of natural killer cell polarity. Recruits CYTIP to early endosomes.2 Publications

GO - Molecular functioni

  • phosphatidylinositol-3-phosphate binding Source: UniProtKB

GO - Biological processi

  • endosomal transport Source: UniProtKB
  • intracellular protein transport Source: UniProtKB
  • nervous system development Source: RGD
  • response to drug Source: RGD
  • retrograde transport, endosome to plasma membrane Source: UniProtKB
  • signal transduction Source: InterPro
Complete GO annotation...

Keywords - Biological processi

Protein transport, Transport

Keywords - Ligandi

Lipid-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Sorting nexin-27
Alternative name(s):
MAP-responsive gene protein
Methamphetamine-responsive transcript 1 protein
PDZ-protein Mrt1
Gene namesi
Name:Snx27
Synonyms:Mrt1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi628705. Snx27.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: UniProtKB-SubCell
  • early endosome Source: UniProtKB
  • early endosome membrane Source: UniProtKB-SubCell
  • immunological synapse Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Endosome, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi51 – 511Y → L: Abolishes interaction with KCNJ9. 1 Publication
Mutagenesisi220 – 2201K → A: Abolishes phosphatidylinositol-3-phosphate-binding and impairs subcellular location. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 539539Sorting nexin-27PRO_0000315358Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei49 – 491PhosphoserineCombined sources
Modified residuei60 – 601PhosphoserineBy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ8K4V4.
PRIDEiQ8K4V4.

PTM databases

PhosphoSiteiQ8K4V4.

Expressioni

Tissue specificityi

Isoform 1 is predominantly expressed in the testis, whereas isoform 2 is predominant in various brain regions, including, neocortex, paleocortex, striatum, hippocampus, cerebellum and brain stem. Expressed in cells of hematopoietic origin.4 Publications

Inductioni

Isoform 2 is up-regulated by methamphetamine and cocaine in the neocortex, but not by pentobarbital nor by D1 antagonist.1 Publication

Interactioni

Subunit structurei

Core component of the SNX27-retromer, a multiprotein complex composed of SNX27, the WASH complex and the retromer complex. Interacts (via PDZ domain) with a number of target transmembrane proteins (via PDZ-binding motif): ABCC4, ADRB2, ARHGEF7, GRIA1, GRIA2, GRIN1, GRIN2A GRIN2C, KCNJ6, KCNJ9 and SLC2A1/GLUT1. Interacts (via the FERM-like regions) with the WASH complex. Interacts with SNX1. Interacts with CYTIP. Interacts with DGKZ. Interacts with MCC.2 Publications

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000067026.

Structurei

Secondary structure

1
539
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi40 – 456Combined sources
Beta strandi50 – 6112Combined sources
Beta strandi66 – 683Combined sources
Beta strandi71 – 744Combined sources
Beta strandi78 – 825Combined sources
Helixi87 – 915Combined sources
Beta strandi98 – 1025Combined sources
Helixi112 – 1198Combined sources
Beta strandi122 – 13110Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3QDOX-ray1.88A39-133[»]
3QE1X-ray1.68A39-133[»]
3QGLX-ray3.31A/B/C/D/E39-133[»]
4P2AX-ray2.70B39-133[»]
4Z8JX-ray0.95A39-133[»]
ProteinModelPortaliQ8K4V4.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ8K4V4.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini41 – 13494PDZPROSITE-ProRule annotationAdd
BLAST
Domaini159 – 267109PXPROSITE-ProRule annotationAdd
BLAST
Domaini271 – 36090Ras-associatingPROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni271 – 36090FERM-like region F1By similarityAdd
BLAST
Regioni371 – 41949FERM-like region F2By similarityAdd
BLAST
Regioni423 – 523101FERM-like region F3By similarityAdd
BLAST

Domaini

The PDZ domain mediates binding to a subset of proteins containing a PDZ-binding motif at the C-terminus: the specificity for PDZ-binding motif is provided by the 2 residues located upstream of the canonical PDZ-binding motif (PubMed:17828261, PubMed:21422294). The PDZ domain also mediates binding to the retromer complex via direct interaction with VPS26 (VPS26A or VPS26B).2 Publications
The PX domain mediates binding to phosphatidylinositol 3-phosphate (PtdIns(3P)) and localization to early endosome membranes.1 Publication

Sequence similaritiesi

Contains 1 PDZ (DHR) domain.PROSITE-ProRule annotation
Contains 1 PX (phox homology) domain.PROSITE-ProRule annotation
Contains 1 Ras-associating domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiENOG410IMRD. Eukaryota.
ENOG410XPAD. LUCA.
HOGENOMiHOG000016641.
HOVERGENiHBG059206.
InParanoidiQ8K4V4.
KOiK17936.
PhylomeDBiQ8K4V4.

Family and domain databases

Gene3Di2.30.42.10. 1 hit.
3.30.1520.10. 1 hit.
InterProiIPR001478. PDZ.
IPR001683. Phox.
IPR000159. RA_dom.
IPR028667. SNX27.
IPR029071. Ubiquitin-rel_dom.
[Graphical view]
PANTHERiPTHR12431:SF17. PTHR12431:SF17. 1 hit.
PfamiPF00595. PDZ. 1 hit.
PF00787. PX. 1 hit.
PF00788. RA. 1 hit.
[Graphical view]
SMARTiSM00228. PDZ. 1 hit.
SM00312. PX. 1 hit.
[Graphical view]
SUPFAMiSSF50156. SSF50156. 1 hit.
SSF54236. SSF54236. 1 hit.
SSF64268. SSF64268. 1 hit.
PROSITEiPS50106. PDZ. 1 hit.
PS50195. PX. 1 hit.
PS50200. RA. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8K4V4-1) [UniParc]FASTAAdd to basket

Also known as: Mrt1alpha, Mrt1a, SNX27a

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MADEDGEGIH PSTPHRNGGG GGGSGLHCAG NGGGGGGGPR VVRIVKSESG
60 70 80 90 100
YGFNVRGQVS EGGQLRSING ELYAPLQHVS AVLPGGAADR AGVRKGDRIL
110 120 130 140 150
EVNGVNVEGA THKQVVDLIR AGEKELILTV LSVPPHEADN LDPSDDSLGQ
160 170 180 190 200
SFYDYTEKQA VPISVPTYKH VEQNGEKFVV YNVYMAGRQL CSKRYREFAI
210 220 230 240 250
LHQNLKREFA NFTFPRLPGK WPFSLSEQQL DARRRGLEEY LEKVCSIRVI
260 270 280 290 300
GESDIMQEFL SESDENYNGV SDVELRVALP DGATVTVRVK KNSTTDQVYQ
310 320 330 340 350
AIAAKVGMDS TTVNYFALFE VINHSFVRKL APNEFPHKLY VQNYTSAVPG
360 370 380 390 400
TCLTIRKWLF TTEEEVLLND NDLAVTYFFH QAVDDVKKGY IKAEEKSYQL
410 420 430 440 450
QKLYEQRKMV MYLNMLRTCE GYNEIIFPHC ACDSRRKGHV ITAISITHFK
460 470 480 490 500
LHACTEEGQL ENQVIAFEWD EMQRWDTDEE GMAFCFEYAR GEKKPRWVKI
510 520 530
FTPYFNYMHE CFERVFCELK WRKENIFQMA RSQQRDVAT
Length:539
Mass (Da):61,015
Last modified:January 15, 2008 - v2
Checksum:iB5EAAD425FCD19F4
GO
Isoform 2 (identifier: Q8K4V4-2) [UniParc]FASTAAdd to basket

Also known as: Mrt1beta, Mrt1b, SNX27b

The sequence of this isoform differs from the canonical sequence as follows:
     525-539: NIFQMARSQQRDVAT → EY

Show »
Length:526
Mass (Da):59,560
Checksum:i5C9651E2788C5F09
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti236 – 2361G → E in BAC10332 (PubMed:12740601).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei525 – 53915NIFQM…RDVAT → EY in isoform 2. 1 PublicationVSP_030541Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB010245 mRNA. Translation: BAC10332.1.
AB051816 mRNA. Translation: BAC10333.1.
RefSeqiNP_001103621.1. NM_001110151.1.
NP_690060.2. NM_152847.2.
UniGeneiRn.7581.

Genome annotation databases

GeneIDi260323.
KEGGirno:260323.
UCSCiRGD:628705. rat. [Q8K4V4-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB010245 mRNA. Translation: BAC10332.1.
AB051816 mRNA. Translation: BAC10333.1.
RefSeqiNP_001103621.1. NM_001110151.1.
NP_690060.2. NM_152847.2.
UniGeneiRn.7581.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3QDOX-ray1.88A39-133[»]
3QE1X-ray1.68A39-133[»]
3QGLX-ray3.31A/B/C/D/E39-133[»]
4P2AX-ray2.70B39-133[»]
4Z8JX-ray0.95A39-133[»]
ProteinModelPortaliQ8K4V4.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000067026.

PTM databases

PhosphoSiteiQ8K4V4.

Proteomic databases

PaxDbiQ8K4V4.
PRIDEiQ8K4V4.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi260323.
KEGGirno:260323.
UCSCiRGD:628705. rat. [Q8K4V4-1]

Organism-specific databases

CTDi81609.
RGDi628705. Snx27.

Phylogenomic databases

eggNOGiENOG410IMRD. Eukaryota.
ENOG410XPAD. LUCA.
HOGENOMiHOG000016641.
HOVERGENiHBG059206.
InParanoidiQ8K4V4.
KOiK17936.
PhylomeDBiQ8K4V4.

Miscellaneous databases

EvolutionaryTraceiQ8K4V4.
NextBioi624357.
PROiQ8K4V4.

Family and domain databases

Gene3Di2.30.42.10. 1 hit.
3.30.1520.10. 1 hit.
InterProiIPR001478. PDZ.
IPR001683. Phox.
IPR000159. RA_dom.
IPR028667. SNX27.
IPR029071. Ubiquitin-rel_dom.
[Graphical view]
PANTHERiPTHR12431:SF17. PTHR12431:SF17. 1 hit.
PfamiPF00595. PDZ. 1 hit.
PF00787. PX. 1 hit.
PF00788. RA. 1 hit.
[Graphical view]
SMARTiSM00228. PDZ. 1 hit.
SM00312. PX. 1 hit.
[Graphical view]
SUPFAMiSSF50156. SSF50156. 1 hit.
SSF54236. SSF54236. 1 hit.
SSF64268. SSF64268. 1 hit.
PROSITEiPS50106. PDZ. 1 hit.
PS50195. PX. 1 hit.
PS50200. RA. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "A developmentally regulated and psychostimulant-inducible novel rat gene mrt1 encoding PDZ-PX proteins isolated in the neocortex."
    Kajii Y., Muraoka S., Hiraoka S., Fujiyama K., Umino A., Nishikawa T.
    Mol. Psychiatry 8:434-444(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2), TISSUE SPECIFICITY, INDUCTION.
    Strain: Wistar.
    Tissue: Brain cortex.
  2. "Subunit-specific regulation of Kir3 channels by sorting nexin 27."
    Nassirpour R., Slesinger P.A.
    Channels 1:331-333(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  3. "Proteomics identification of sorting nexin 27 as a diacylglycerol kinase zeta-associated protein: new diacylglycerol kinase roles in endocytic recycling."
    Rincon E., Santos T., Avila-Flores A., Albar J.P., Lalioti V., Lei C., Hong W., Merida I.
    Mol. Cell. Proteomics 6:1073-1087(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY.
  4. "A unique sorting nexin regulates trafficking of potassium channels via a PDZ domain interaction."
    Lunn M.L., Nassirpour R., Arrabit C., Tan J., McLeod I., Arias C.M., Sawchenko P.E., Yates J.R. III, Slesinger P.A.
    Nat. Neurosci. 10:1249-1259(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH KCNJ6 AND KCNJ9, TISSUE SPECIFICITY, PHOSPHATIDYLINOSITOL-3-PHOSPHATE-BINDING, MUTAGENESIS OF LYS-220.
  5. "Quantitative maps of protein phosphorylation sites across 14 different rat organs and tissues."
    Lundby A., Secher A., Lage K., Nordsborg N.B., Dmytriyev A., Lundby C., Olsen J.V.
    Nat. Commun. 3:876-876(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-49, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  6. "Mechanism underlying selective regulation of G protein-gated inwardly rectifying potassium channels by the psychostimulant-sensitive sorting nexin 27."
    Balana B., Maslennikov I., Kwiatkowski W., Stern K.M., Bahima L., Choe S., Slesinger P.A.
    Proc. Natl. Acad. Sci. U.S.A. 108:5831-5836(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.68 ANGSTROMS) OF 36-133 IN COMPLEX WITH KCNJ9, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, INTERACTION WITH KCNJ9, MUTAGENESIS OF TYR-51.

Entry informationi

Entry nameiSNX27_RAT
AccessioniPrimary (citable) accession number: Q8K4V4
Secondary accession number(s): Q8K4T6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: January 15, 2008
Last modified: May 11, 2016
This is version 86 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.