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Protein

STE20-related kinase adapter protein beta

Gene

Stradb

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Pseudokinase which, in complex with CAB39/MO25 (CAB39/MO25alpha or CAB39L/MO25beta), binds to and activates STK11/LKB1. Adopts a closed conformation typical of active protein kinases and binds STK11/LKB1 as a pseudosubstrate, promoting conformational change of STK11/LKB1 in an active conformation (By similarity).By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei89 – 891ATPPROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi64 – 729ATPPROSITE-ProRule annotation

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • protein kinase activity Source: MGI

GO - Biological processi

  • activation of protein kinase activity Source: UniProtKB
  • cell cycle Source: UniProtKB-KW
  • cell morphogenesis Source: MGI
  • cytoskeleton organization Source: MGI
  • JNK cascade Source: MGI
  • negative regulation of extrinsic apoptotic signaling pathway in absence of ligand Source: MGI
  • protein export from nucleus Source: UniProtKB
Complete GO annotation...

Keywords - Biological processi

Cell cycle

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-MMU-380972. Energy dependent regulation of mTOR by LKB1-AMPK.

Names & Taxonomyi

Protein namesi
Recommended name:
STE20-related kinase adapter protein beta
Short name:
STRAD beta
Alternative name(s):
Amyotrophic lateral sclerosis 2 chromosomal region candidate gene 2 protein homolog
ILP-interacting protein homolog
Polyploidy-associated protein kinase
Pseudokinase ALS2CR2
Gene namesi
Name:Stradb
Synonyms:Als2cr2, Papk, Syradb
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 1

Organism-specific databases

MGIiMGI:2144047. Stradb.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 418418STE20-related kinase adapter protein betaPRO_0000085618Add
BLAST

Proteomic databases

MaxQBiQ8K4T3.
PaxDbiQ8K4T3.
PRIDEiQ8K4T3.

PTM databases

iPTMnetiQ8K4T3.
PhosphoSiteiQ8K4T3.

Expressioni

Gene expression databases

BgeeiQ8K4T3.
ExpressionAtlasiQ8K4T3. baseline and differential.
GenevisibleiQ8K4T3. MM.

Interactioni

Subunit structurei

Component of a trimeric complex composed of STK11/LKB1, STRAD (STRADA or STRADB) and CAB39/MO25 (CAB39/MO25alpha or CAB39L/MO25beta): the complex tethers STK11/LKB1 in the cytoplasm and stimulates its catalytic activity. Interacts with BIRC4/XIAP. These two proteins are likely to coexist in a complex with TAK1, TRAF6, TAB1 and TAB2 (By similarity).By similarity

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000027185.

Structurei

3D structure databases

ProteinModelPortaliQ8K4T3.
SMRiQ8K4T3. Positions 3-418.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini58 – 369312Protein kinasePROSITE-ProRule annotationAdd
BLAST

Domaini

The protein kinase domain is predicted to be catalytically inactive.

Sequence similaritiesi

Contains 1 protein kinase domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0582. Eukaryota.
ENOG410XSWS. LUCA.
GeneTreeiENSGT00840000129768.
HOGENOMiHOG000237355.
HOVERGENiHBG055069.
InParanoidiQ8K4T3.
KOiK17532.
OMAiSDRLHTP.
OrthoDBiEOG71G9V6.
PhylomeDBiQ8K4T3.
TreeFamiTF319817.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 2 hits.
PROSITEiPS50011. PROTEIN_KINASE_DOM. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8K4T3-1) [UniParc]FASTAAdd to basket

Also known as: PAPK-A

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSLLDCFCAS RTRVESLRPE KQSETSIHQY LVDESAISRP PPSARASEVI
60 70 80 90 100
CSTDVSHYEL QVEIGRGFDN LTSVHLARHT PTGTLVTVKI TNLESCTEER
110 120 130 140 150
LKALQRAVIL SHFFQHPNIT TYWTVFTVGS WLWVISPFMA YGSASQLLRT
160 170 180 190 200
YFPDGMSETL IRNILFGAVQ GLNYLHQNGC IHRSFKASHI LISGDGLVTL
210 220 230 240 250
SGLSHLHSLL KHGQRHRAVF DFPQFSTSVQ PWLSPELLRQ DLHGYNVKSD
260 270 280 290 300
IYSVGITACE LASGQVPFQD MHRTQMLLQK LKGPPYSPLD VSIFPQSDSR
310 320 330 340 350
MRNSQSGVDS GIGESVLVST GTHTVNSDRL HTPSTKTFSP AFFSLVQLCL
360 370 380 390 400
QQDPEKRPSA SSLLSHVFFK QMKEESQDSI LPLLPPAYNR PSASLQPVSP
410
WSELEFQFPD DKDPVWEF
Length:418
Mass (Da):46,832
Last modified:October 1, 2002 - v1
Checksum:i4EB7FAAEF9F87E0A
GO
Isoform 2 (identifier: Q8K4T3-2) [UniParc]FASTAAdd to basket

Also known as: PAPK-B

The sequence of this isoform differs from the canonical sequence as follows:
     184-209: SFKASHILISGDGLVTLSGLSHLHSL → YLLIYSLHKRKNFKEVIQRNFCKDKC
     210-418: Missing.

Show »
Length:209
Mass (Da):23,930
Checksum:i733A4244C3B77BDF
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti184 – 1896SFKASH → IYMDIM in BAB68236 (PubMed:12574163).Curated
Sequence conflicti184 – 1896SFKASH → IYMDIM in BAE24309 (PubMed:16141072).Curated
Sequence conflicti343 – 3431F → L in AAH60956 (PubMed:15489334).Curated
Sequence conflicti345 – 3451L → S in BAC32870 (PubMed:16141072).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei184 – 20926SFKAS…HLHSL → YLLIYSLHKRKNFKEVIQRN FCKDKC in isoform 2. 1 PublicationVSP_016626Add
BLAST
Alternative sequencei210 – 418209Missing in isoform 2. 1 PublicationVSP_016627Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB057666 mRNA. Translation: BAB68235.1.
AB057667 mRNA. Translation: BAB68236.1.
AK046790 mRNA. Translation: BAC32870.1.
AK080096 mRNA. Translation: BAC37825.1.
AK086974 mRNA. Translation: BAC39775.1.
AK146079 mRNA. Translation: BAE26883.1.
AK140272 mRNA. Translation: BAE24309.1.
BC060956 mRNA. Translation: AAH60956.1.
CCDSiCCDS14981.1. [Q8K4T3-1]
RefSeqiNP_766244.4. NM_172656.5. [Q8K4T3-1]
UniGeneiMm.286006.

Genome annotation databases

EnsembliENSMUST00000027185; ENSMUSP00000027185; ENSMUSG00000026027. [Q8K4T3-1]
ENSMUST00000114296; ENSMUSP00000109935; ENSMUSG00000026027. [Q8K4T3-2]
GeneIDi227154.
KEGGimmu:227154.
UCSCiuc007bcx.1. mouse. [Q8K4T3-2]
uc007bcy.1. mouse. [Q8K4T3-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB057666 mRNA. Translation: BAB68235.1.
AB057667 mRNA. Translation: BAB68236.1.
AK046790 mRNA. Translation: BAC32870.1.
AK080096 mRNA. Translation: BAC37825.1.
AK086974 mRNA. Translation: BAC39775.1.
AK146079 mRNA. Translation: BAE26883.1.
AK140272 mRNA. Translation: BAE24309.1.
BC060956 mRNA. Translation: AAH60956.1.
CCDSiCCDS14981.1. [Q8K4T3-1]
RefSeqiNP_766244.4. NM_172656.5. [Q8K4T3-1]
UniGeneiMm.286006.

3D structure databases

ProteinModelPortaliQ8K4T3.
SMRiQ8K4T3. Positions 3-418.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000027185.

PTM databases

iPTMnetiQ8K4T3.
PhosphoSiteiQ8K4T3.

Proteomic databases

MaxQBiQ8K4T3.
PaxDbiQ8K4T3.
PRIDEiQ8K4T3.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000027185; ENSMUSP00000027185; ENSMUSG00000026027. [Q8K4T3-1]
ENSMUST00000114296; ENSMUSP00000109935; ENSMUSG00000026027. [Q8K4T3-2]
GeneIDi227154.
KEGGimmu:227154.
UCSCiuc007bcx.1. mouse. [Q8K4T3-2]
uc007bcy.1. mouse. [Q8K4T3-1]

Organism-specific databases

CTDi55437.
MGIiMGI:2144047. Stradb.

Phylogenomic databases

eggNOGiKOG0582. Eukaryota.
ENOG410XSWS. LUCA.
GeneTreeiENSGT00840000129768.
HOGENOMiHOG000237355.
HOVERGENiHBG055069.
InParanoidiQ8K4T3.
KOiK17532.
OMAiSDRLHTP.
OrthoDBiEOG71G9V6.
PhylomeDBiQ8K4T3.
TreeFamiTF319817.

Enzyme and pathway databases

ReactomeiR-MMU-380972. Energy dependent regulation of mTOR by LKB1-AMPK.

Miscellaneous databases

PROiQ8K4T3.
SOURCEiSearch...

Gene expression databases

BgeeiQ8K4T3.
ExpressionAtlasiQ8K4T3. baseline and differential.
GenevisibleiQ8K4T3. MM.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 2 hits.
PROSITEiPS50011. PROTEIN_KINASE_DOM. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Identification and characterization of a novel Ste20/germinal center kinase-related kinase, polyploidy-associated protein kinase."
    Nishigaki K., Thompson D., Yugawa T., Rulli K., Hanson C., Cmarik J., Gutkind J.S., Teramoto H., Ruscetti S.
    J. Biol. Chem. 278:13520-13530(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
  2. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
    Strain: C57BL/6J.
    Tissue: Corpora quadrigemina, Lung and Medulla oblongata.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Tissue: Liver.

Entry informationi

Entry nameiSTRAB_MOUSE
AccessioniPrimary (citable) accession number: Q8K4T3
Secondary accession number(s): Q6P922
, Q8BU86, Q8BXK0, Q8K4T2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: October 1, 2002
Last modified: June 8, 2016
This is version 117 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Caution

Ser-184 is present instead of the conserved Asp which is expected to be an active site residue.Curated

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. Human and mouse protein kinases
    Human and mouse protein kinases: classification and index
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.