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Q8K4T1 (SIA8F_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 90. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Web links·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Alpha-2,8-sialyltransferase 8F

EC=2.4.99.-
Alternative name(s):
Sialyltransferase 8F
Short name=SIAT8-F
Sialyltransferase St8Sia VI
Short name=ST8SiaVI
Gene names
Name:St8sia6
Synonyms:Siat8f
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length398 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Prefers O-glycans to N-glycans or glycolipids as acceptor substrates. The minimal acceptor substrate is the NeuAc-alpha-2,36-Gal sequence at the non-reducing end of their carbohydrate groups.

Pathway

Protein modification; protein glycosylation.

Subcellular location

Golgi apparatus membrane; Single-pass type II membrane protein Potential.

Sequence similarities

Belongs to the glycosyltransferase 29 family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 398398Alpha-2,8-sialyltransferase 8F
PRO_0000149300

Regions

Topological domain1 – 33Cytoplasmic Potential
Transmembrane4 – 2421Helical; Signal-anchor for type II membrane protein; Potential
Topological domain25 – 398374Lumenal Potential

Amino acid modifications

Glycosylation661N-linked (GlcNAc...) Potential
Glycosylation931N-linked (GlcNAc...) Potential
Glycosylation1511N-linked (GlcNAc...) Potential
Glycosylation1961N-linked (GlcNAc...) Potential
Disulfide bond186 ↔ 335 By similarity

Sequences

Sequence LengthMass (Da)Tools
Q8K4T1 [UniParc].

Last modified October 1, 2002. Version 1.
Checksum: A73A7A29640D4917

FASTA39845,428
        10         20         30         40         50         60 
MRSGGTLFAL IGSLMLLLLL RMLWCPADAP ARSRLLMEGS REDTSGTSAA LKTLWSPTTP 

        70         80         90        100        110        120 
VPRTRNSTYL DEKTTQITEK CKDLQYSLNS LSNKTRRYSE DDYLQTITNI QRCPWNRQAE 

       130        140        150        160        170        180 
EYDNFRAKLA SCCDAIQDFV VSQNNTPVGT NMSYEVESKK HIPIRENIFH MFPVSQPFVD 

       190        200        210        220        230        240 
YPYNQCAVVG NGGILNKSLC GAEIDKSDFV FRCNLPPITG SASKDVGSKT NLVTVNPSII 

       250        260        270        280        290        300 
TLKYQNLKEK KAQFLEDIST YGDAFLLLPA FSYRANTGIS FKVYQTLKES KMRQKVLFFH 

       310        320        330        340        350        360 
PRYLRHLALF WRTKGVTAYR LSTGLMIASV AVELCENVKL YGFWPFSKTI EDTPLSHHYY 

       370        380        390 
DNMLPKHGFH QMPKEYSQML QLHMRGILKL QFSKCETA 

« Hide

References

[1]"Molecular cloning and expression of a sixth type of alpha 2,8-sialyltransferase (ST8Sia VI) that sialylates O-glycans."
Takashima S., Ishida H.K., Inazu T., Ando T., Ishida H., Kiso M., Tsuji S., Tsujimoto M.
J. Biol. Chem. 277:24030-24038(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], CHARACTERIZATION.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AB059554 mRNA. Translation: BAC01265.1.
CCDSCCDS15697.1.
RefSeqNP_665837.1. NM_145838.1.
UniGeneMm.330004.
Mm.393036.

3D structure databases

ProteinModelPortalQ8K4T1.
SMRQ8K4T1. Positions 182-358.
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

CAZyGT29. Glycosyltransferase Family 29.

PTM databases

PhosphoSiteQ8K4T1.

Proteomic databases

PaxDbQ8K4T1.
PRIDEQ8K4T1.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000003509; ENSMUSP00000003509; ENSMUSG00000003418.
GeneID241230.
KEGGmmu:241230.
UCSCuc008ikc.2. mouse.

Organism-specific databases

CTD338596.
MGIMGI:2386797. St8sia6.

Phylogenomic databases

eggNOGNOG323775.
GeneTreeENSGT00550000074407.
HOVERGENHBG106106.
InParanoidQ8K4T1.
KOK06615.
OMANMSYEVE.
OrthoDBEOG7SV0V9.
TreeFamTF323961.

Enzyme and pathway databases

UniPathwayUPA00378.

Gene expression databases

ArrayExpressQ8K4T1.
BgeeQ8K4T1.
GenevestigatorQ8K4T1.

Family and domain databases

InterProIPR001675. Glyco_trans_29.
IPR012163. Sialyl_trans.
[Graphical view]
PfamPF00777. Glyco_transf_29. 1 hit.
[Graphical view]
PIRSFPIRSF005557. Sialyl_trans. 1 hit.
ProtoNetSearch...

Other

NextBio384941.
PROQ8K4T1.
SOURCESearch...

Entry information

Entry nameSIA8F_MOUSE
AccessionPrimary (citable) accession number: Q8K4T1
Entry history
Integrated into UniProtKB/Swiss-Prot: June 7, 2004
Last sequence update: October 1, 2002
Last modified: July 9, 2014
This is version 90 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot