Q8K4T1 (SIA8F_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Alpha-2,8-sialyltransferase 8F
EC=2.4.99.-

Alternative name(s):
Sialyltransferase 8F
Short name=SIAT8-F
Sialyltransferase St8Sia VI
Short name=ST8SiaVI

Gene names

Name:	St8sia6
Synonyms:	Siat8f

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	398 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Prefers O-glycans to N-glycans or glycolipids as acceptor substrates. The minimal acceptor substrate is the NeuAc-alpha-2,36-Gal sequence at the non-reducing end of their carbohydrate groups.
Pathway	Protein modification; protein glycosylation.
Subcellular location	Golgi apparatus membrane; Single-pass type II membrane protein Potential.
Sequence similarities	Belongs to the glycosyltransferase 29 family.

Ontologies

Keywords
Cellular component	Golgi apparatus Membrane
Domain	Signal-anchor Transmembrane Transmembrane helix
Molecular function	Glycosyltransferase Transferase
PTM	Disulfide bond Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	carbohydrate biosynthetic process Inferred from direct assay Ref.1. Source: MGI ganglioside biosynthetic process Inferred from direct assay Ref.1. Source: BHF-UCL oligosaccharide metabolic process Inferred from direct assay Ref.1. Source: BHF-UCL protein O-linked glycosylation Inferred from direct assay Ref.1. Source: MGI
Cellular_component	integral to Golgi membrane Inferred from electronic annotation. Source: InterPro
Molecular_function	sialyltransferase activity Inferred from direct assay Ref.1. Source: MGI
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 398	398	Alpha-2,8-sialyltransferase 8F		PRO_0000149300
Regions
	Topological domain	1 – 3	3	Cytoplasmic Potential
	Transmembrane	4 – 24	21	Helical; Signal-anchor for type II membrane protein; Potential
	Topological domain	25 – 398	374	Lumenal Potential
Amino acid modifications
	Glycosylation	66	1	N-linked (GlcNAc...) Potential
	Glycosylation	93	1	N-linked (GlcNAc...) Potential
	Glycosylation	151	1	N-linked (GlcNAc...) Potential
	Glycosylation	196	1	N-linked (GlcNAc...) Potential
	Disulfide bond	186 ↔ 335		By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8K4T1 [UniParc]. Last modified October 1, 2002. Version 1. Checksum: A73A7A29640D4917	FASTA	398	45,428
	10 20 30 40 50 60 MRSGGTLFAL IGSLMLLLLL RMLWCPADAP ARSRLLMEGS REDTSGTSAA LKTLWSPTTP 70 80 90 100 110 120 VPRTRNSTYL DEKTTQITEK CKDLQYSLNS LSNKTRRYSE DDYLQTITNI QRCPWNRQAE 130 140 150 160 170 180 EYDNFRAKLA SCCDAIQDFV VSQNNTPVGT NMSYEVESKK HIPIRENIFH MFPVSQPFVD 190 200 210 220 230 240 YPYNQCAVVG NGGILNKSLC GAEIDKSDFV FRCNLPPITG SASKDVGSKT NLVTVNPSII 250 260 270 280 290 300 TLKYQNLKEK KAQFLEDIST YGDAFLLLPA FSYRANTGIS FKVYQTLKES KMRQKVLFFH 310 320 330 340 350 360 PRYLRHLALF WRTKGVTAYR LSTGLMIASV AVELCENVKL YGFWPFSKTI EDTPLSHHYY 370 380 390 DNMLPKHGFH QMPKEYSQML QLHMRGILKL QFSKCETA

References

[1]	"Molecular cloning and expression of a sixth type of alpha 2,8-sialyltransferase (ST8Sia VI) that sialylates O-glycans." Takashima S., Ishida H.K., Inazu T., Ando T., Ishida H., Kiso M., Tsuji S., Tsujimoto M. J. Biol. Chem. 277:24030-24038(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], CHARACTERIZATION.
+	Additional computationally mapped references.

Web resources

Functional Glycomics Gateway - GTase

ST8Sia VI

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB059554 mRNA. Translation: BAC01265.1.
IPI	IPI00170272.
RefSeq	NP_665837.1. NM_145838.1.
UniGene	Mm.330004. Mm.393036.
3D structure databases
ProteinModelPortal	Q8K4T1.
SMR	Q8K4T1. Positions 99-394.
ModBase	Search...
Protein family/group databases
CAZy	GT29. Glycosyltransferase Family 29.
PTM databases
PhosphoSite	Q8K4T1.
Proteomic databases
PaxDb	Q8K4T1.
PRIDE	Q8K4T1.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000003509; ENSMUSP00000003509; ENSMUSG00000003418.
GeneID	241230.
KEGG	mmu:241230.
Organism-specific databases
CTD	338596.
MGI	MGI:2386797. St8sia6.
Phylogenomic databases
eggNOG	NOG323775.
GeneTree	ENSGT00550000074407.
HOVERGEN	HBG106106.
InParanoid	Q8K4T1.
KO	K06615.
OMA	NMSYEVE.
OrthoDB	EOG4HMJ9H.
Enzyme and pathway databases
UniPathway	UPA00378.
Gene expression databases
ArrayExpress	Q8K4T1.
Bgee	Q8K4T1.
Genevestigator	Q8K4T1.
GermOnline	ENSMUSG00000003418. Mus musculus.
Family and domain databases
InterPro	IPR001675. Glyco_trans_29. IPR012163. Sialyl_trans. [Graphical view]
Pfam	PF00777. Glyco_transf_29. 1 hit. [Graphical view]
PIRSF	PIRSF005557. Sialyl_trans. 1 hit.
ProtoNet	Search...
Other
NextBio	384941.
SOURCE	Search...

Entry information

Entry name

SIA8F_MOUSE

Accession

Primary (citable) accession number: Q8K4T1

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 7, 2004
Last sequence update:	October 1, 2002
Last modified:	April 3, 2013
This is version 81 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program