Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Alpha-2,8-sialyltransferase 8F

Gene

St8sia6

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Prefers O-glycans to N-glycans or glycolipids as acceptor substrates. The minimal acceptor substrate is the NeuAc-alpha-2,36-Gal sequence at the non-reducing end of their carbohydrate groups.

Pathwayi

GO - Molecular functioni

  1. sialyltransferase activity Source: MGI

GO - Biological processi

  1. carbohydrate biosynthetic process Source: MGI
  2. ganglioside biosynthetic process Source: BHF-UCL
  3. glycolipid biosynthetic process Source: MGI
  4. glycoprotein metabolic process Source: BHF-UCL
  5. oligosaccharide metabolic process Source: BHF-UCL
  6. protein O-linked glycosylation Source: MGI
  7. sialylation Source: GOC
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Enzyme and pathway databases

BRENDAi2.4.99.8. 3474.
ReactomeiREACT_308816. N-Glycan antennae elongation.
UniPathwayiUPA00378.

Protein family/group databases

CAZyiGT29. Glycosyltransferase Family 29.

Names & Taxonomyi

Protein namesi
Recommended name:
Alpha-2,8-sialyltransferase 8F (EC:2.4.99.-)
Alternative name(s):
Sialyltransferase 8F
Short name:
SIAT8-F
Sialyltransferase St8Sia VI
Short name:
ST8SiaVI
Gene namesi
Name:St8sia6
Synonyms:Siat8f
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 2

Organism-specific databases

MGIiMGI:2386797. St8sia6.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 33CytoplasmicSequence Analysis
Transmembranei4 – 2421Helical; Signal-anchor for type II membrane proteinSequence AnalysisAdd
BLAST
Topological domaini25 – 398374LumenalSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. integral component of Golgi membrane Source: InterPro
Complete GO annotation...

Keywords - Cellular componenti

Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 398398Alpha-2,8-sialyltransferase 8FPRO_0000149300Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi66 – 661N-linked (GlcNAc...)Sequence Analysis
Glycosylationi93 – 931N-linked (GlcNAc...)Sequence Analysis
Glycosylationi151 – 1511N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi186 ↔ 335By similarity
Glycosylationi196 – 1961N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiQ8K4T1.
PRIDEiQ8K4T1.

PTM databases

PhosphoSiteiQ8K4T1.

Expressioni

Gene expression databases

BgeeiQ8K4T1.
ExpressionAtlasiQ8K4T1. baseline and differential.
GenevestigatoriQ8K4T1.

Structurei

3D structure databases

ProteinModelPortaliQ8K4T1.
SMRiQ8K4T1. Positions 182-358.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyltransferase 29 family.Curated

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG323775.
GeneTreeiENSGT00550000074407.
HOVERGENiHBG106106.
InParanoidiQ8K4T1.
KOiK06615.
OMAiNMSYEVE.
OrthoDBiEOG7SV0V9.
TreeFamiTF323961.

Family and domain databases

InterProiIPR001675. Glyco_trans_29.
IPR012163. Sialyl_trans.
[Graphical view]
PfamiPF00777. Glyco_transf_29. 1 hit.
[Graphical view]
PIRSFiPIRSF005557. Sialyl_trans. 1 hit.

Sequencei

Sequence statusi: Complete.

Q8K4T1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRSGGTLFAL IGSLMLLLLL RMLWCPADAP ARSRLLMEGS REDTSGTSAA
60 70 80 90 100
LKTLWSPTTP VPRTRNSTYL DEKTTQITEK CKDLQYSLNS LSNKTRRYSE
110 120 130 140 150
DDYLQTITNI QRCPWNRQAE EYDNFRAKLA SCCDAIQDFV VSQNNTPVGT
160 170 180 190 200
NMSYEVESKK HIPIRENIFH MFPVSQPFVD YPYNQCAVVG NGGILNKSLC
210 220 230 240 250
GAEIDKSDFV FRCNLPPITG SASKDVGSKT NLVTVNPSII TLKYQNLKEK
260 270 280 290 300
KAQFLEDIST YGDAFLLLPA FSYRANTGIS FKVYQTLKES KMRQKVLFFH
310 320 330 340 350
PRYLRHLALF WRTKGVTAYR LSTGLMIASV AVELCENVKL YGFWPFSKTI
360 370 380 390
EDTPLSHHYY DNMLPKHGFH QMPKEYSQML QLHMRGILKL QFSKCETA
Length:398
Mass (Da):45,428
Last modified:September 30, 2002 - v1
Checksum:iA73A7A29640D4917
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB059554 mRNA. Translation: BAC01265.1.
CCDSiCCDS15697.1.
RefSeqiNP_665837.1. NM_145838.1.
UniGeneiMm.330004.
Mm.393036.

Genome annotation databases

EnsembliENSMUST00000003509; ENSMUSP00000003509; ENSMUSG00000003418.
GeneIDi241230.
KEGGimmu:241230.
UCSCiuc008ikc.2. mouse.

Cross-referencesi

Web resourcesi

Functional Glycomics Gateway - GTase

ST8Sia VI

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB059554 mRNA. Translation: BAC01265.1.
CCDSiCCDS15697.1.
RefSeqiNP_665837.1. NM_145838.1.
UniGeneiMm.330004.
Mm.393036.

3D structure databases

ProteinModelPortaliQ8K4T1.
SMRiQ8K4T1. Positions 182-358.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

CAZyiGT29. Glycosyltransferase Family 29.

PTM databases

PhosphoSiteiQ8K4T1.

Proteomic databases

PaxDbiQ8K4T1.
PRIDEiQ8K4T1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000003509; ENSMUSP00000003509; ENSMUSG00000003418.
GeneIDi241230.
KEGGimmu:241230.
UCSCiuc008ikc.2. mouse.

Organism-specific databases

CTDi338596.
MGIiMGI:2386797. St8sia6.

Phylogenomic databases

eggNOGiNOG323775.
GeneTreeiENSGT00550000074407.
HOVERGENiHBG106106.
InParanoidiQ8K4T1.
KOiK06615.
OMAiNMSYEVE.
OrthoDBiEOG7SV0V9.
TreeFamiTF323961.

Enzyme and pathway databases

UniPathwayiUPA00378.
BRENDAi2.4.99.8. 3474.
ReactomeiREACT_308816. N-Glycan antennae elongation.

Miscellaneous databases

NextBioi384941.
PROiQ8K4T1.
SOURCEiSearch...

Gene expression databases

BgeeiQ8K4T1.
ExpressionAtlasiQ8K4T1. baseline and differential.
GenevestigatoriQ8K4T1.

Family and domain databases

InterProiIPR001675. Glyco_trans_29.
IPR012163. Sialyl_trans.
[Graphical view]
PfamiPF00777. Glyco_transf_29. 1 hit.
[Graphical view]
PIRSFiPIRSF005557. Sialyl_trans. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "Molecular cloning and expression of a sixth type of alpha 2,8-sialyltransferase (ST8Sia VI) that sialylates O-glycans."
    Takashima S., Ishida H.K., Inazu T., Ando T., Ishida H., Kiso M., Tsuji S., Tsujimoto M.
    J. Biol. Chem. 277:24030-24038(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], CHARACTERIZATION.

Entry informationi

Entry nameiSIA8F_MOUSE
AccessioniPrimary (citable) accession number: Q8K4T1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 6, 2004
Last sequence update: September 30, 2002
Last modified: March 31, 2015
This is version 95 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.