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Q8K4T1

- SIA8F_MOUSE

UniProt

Q8K4T1 - SIA8F_MOUSE

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Protein

Alpha-2,8-sialyltransferase 8F

Gene

St8sia6

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Prefers O-glycans to N-glycans or glycolipids as acceptor substrates. The minimal acceptor substrate is the NeuAc-alpha-2,36-Gal sequence at the non-reducing end of their carbohydrate groups.

Pathwayi

GO - Molecular functioni

  1. sialyltransferase activity Source: MGI

GO - Biological processi

  1. carbohydrate biosynthetic process Source: MGI
  2. ganglioside biosynthetic process Source: BHF-UCL
  3. glycolipid biosynthetic process Source: MGI
  4. glycoprotein metabolic process Source: BHF-UCL
  5. oligosaccharide metabolic process Source: BHF-UCL
  6. protein O-linked glycosylation Source: MGI
  7. sialylation Source: GOC
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Enzyme and pathway databases

ReactomeiREACT_198565. N-Glycan antennae elongation.
UniPathwayiUPA00378.

Protein family/group databases

CAZyiGT29. Glycosyltransferase Family 29.

Names & Taxonomyi

Protein namesi
Recommended name:
Alpha-2,8-sialyltransferase 8F (EC:2.4.99.-)
Alternative name(s):
Sialyltransferase 8F
Short name:
SIAT8-F
Sialyltransferase St8Sia VI
Short name:
ST8SiaVI
Gene namesi
Name:St8sia6
Synonyms:Siat8f
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 2

Organism-specific databases

MGIiMGI:2386797. St8sia6.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 33CytoplasmicSequence Analysis
Transmembranei4 – 2421Helical; Signal-anchor for type II membrane proteinSequence AnalysisAdd
BLAST
Topological domaini25 – 398374LumenalSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. integral component of Golgi membrane Source: InterPro
Complete GO annotation...

Keywords - Cellular componenti

Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 398398Alpha-2,8-sialyltransferase 8FPRO_0000149300Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi66 – 661N-linked (GlcNAc...)Sequence Analysis
Glycosylationi93 – 931N-linked (GlcNAc...)Sequence Analysis
Glycosylationi151 – 1511N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi186 ↔ 335By similarity
Glycosylationi196 – 1961N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiQ8K4T1.
PRIDEiQ8K4T1.

PTM databases

PhosphoSiteiQ8K4T1.

Expressioni

Gene expression databases

BgeeiQ8K4T1.
ExpressionAtlasiQ8K4T1. baseline and differential.
GenevestigatoriQ8K4T1.

Structurei

3D structure databases

ProteinModelPortaliQ8K4T1.
SMRiQ8K4T1. Positions 182-358.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyltransferase 29 family.Curated

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG323775.
GeneTreeiENSGT00550000074407.
HOVERGENiHBG106106.
InParanoidiQ8K4T1.
KOiK06615.
OMAiNMSYEVE.
OrthoDBiEOG7SV0V9.
TreeFamiTF323961.

Family and domain databases

InterProiIPR001675. Glyco_trans_29.
IPR012163. Sialyl_trans.
[Graphical view]
PfamiPF00777. Glyco_transf_29. 1 hit.
[Graphical view]
PIRSFiPIRSF005557. Sialyl_trans. 1 hit.

Sequencei

Sequence statusi: Complete.

Q8K4T1-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MRSGGTLFAL IGSLMLLLLL RMLWCPADAP ARSRLLMEGS REDTSGTSAA
60 70 80 90 100
LKTLWSPTTP VPRTRNSTYL DEKTTQITEK CKDLQYSLNS LSNKTRRYSE
110 120 130 140 150
DDYLQTITNI QRCPWNRQAE EYDNFRAKLA SCCDAIQDFV VSQNNTPVGT
160 170 180 190 200
NMSYEVESKK HIPIRENIFH MFPVSQPFVD YPYNQCAVVG NGGILNKSLC
210 220 230 240 250
GAEIDKSDFV FRCNLPPITG SASKDVGSKT NLVTVNPSII TLKYQNLKEK
260 270 280 290 300
KAQFLEDIST YGDAFLLLPA FSYRANTGIS FKVYQTLKES KMRQKVLFFH
310 320 330 340 350
PRYLRHLALF WRTKGVTAYR LSTGLMIASV AVELCENVKL YGFWPFSKTI
360 370 380 390
EDTPLSHHYY DNMLPKHGFH QMPKEYSQML QLHMRGILKL QFSKCETA
Length:398
Mass (Da):45,428
Last modified:October 1, 2002 - v1
Checksum:iA73A7A29640D4917
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB059554 mRNA. Translation: BAC01265.1.
CCDSiCCDS15697.1.
RefSeqiNP_665837.1. NM_145838.1.
UniGeneiMm.330004.
Mm.393036.

Genome annotation databases

EnsembliENSMUST00000003509; ENSMUSP00000003509; ENSMUSG00000003418.
GeneIDi241230.
KEGGimmu:241230.
UCSCiuc008ikc.2. mouse.

Cross-referencesi

Web resourcesi

Functional Glycomics Gateway - GTase

ST8Sia VI

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB059554 mRNA. Translation: BAC01265.1 .
CCDSi CCDS15697.1.
RefSeqi NP_665837.1. NM_145838.1.
UniGenei Mm.330004.
Mm.393036.

3D structure databases

ProteinModelPortali Q8K4T1.
SMRi Q8K4T1. Positions 182-358.
ModBasei Search...
MobiDBi Search...

Protein family/group databases

CAZyi GT29. Glycosyltransferase Family 29.

PTM databases

PhosphoSitei Q8K4T1.

Proteomic databases

PaxDbi Q8K4T1.
PRIDEi Q8K4T1.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000003509 ; ENSMUSP00000003509 ; ENSMUSG00000003418 .
GeneIDi 241230.
KEGGi mmu:241230.
UCSCi uc008ikc.2. mouse.

Organism-specific databases

CTDi 338596.
MGIi MGI:2386797. St8sia6.

Phylogenomic databases

eggNOGi NOG323775.
GeneTreei ENSGT00550000074407.
HOVERGENi HBG106106.
InParanoidi Q8K4T1.
KOi K06615.
OMAi NMSYEVE.
OrthoDBi EOG7SV0V9.
TreeFami TF323961.

Enzyme and pathway databases

UniPathwayi UPA00378 .
Reactomei REACT_198565. N-Glycan antennae elongation.

Miscellaneous databases

NextBioi 384941.
PROi Q8K4T1.
SOURCEi Search...

Gene expression databases

Bgeei Q8K4T1.
ExpressionAtlasi Q8K4T1. baseline and differential.
Genevestigatori Q8K4T1.

Family and domain databases

InterProi IPR001675. Glyco_trans_29.
IPR012163. Sialyl_trans.
[Graphical view ]
Pfami PF00777. Glyco_transf_29. 1 hit.
[Graphical view ]
PIRSFi PIRSF005557. Sialyl_trans. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Molecular cloning and expression of a sixth type of alpha 2,8-sialyltransferase (ST8Sia VI) that sialylates O-glycans."
    Takashima S., Ishida H.K., Inazu T., Ando T., Ishida H., Kiso M., Tsuji S., Tsujimoto M.
    J. Biol. Chem. 277:24030-24038(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], CHARACTERIZATION.

Entry informationi

Entry nameiSIA8F_MOUSE
AccessioniPrimary (citable) accession number: Q8K4T1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 7, 2004
Last sequence update: October 1, 2002
Last modified: October 29, 2014
This is version 93 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3