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Q8K4T1

- SIA8F_MOUSE

UniProt

Q8K4T1 - SIA8F_MOUSE

Protein

Alpha-2,8-sialyltransferase 8F

Gene

St8sia6

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 92 (01 Oct 2014)
      Sequence version 1 (01 Oct 2002)
      Previous versions | rss
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    Functioni

    Prefers O-glycans to N-glycans or glycolipids as acceptor substrates. The minimal acceptor substrate is the NeuAc-alpha-2,36-Gal sequence at the non-reducing end of their carbohydrate groups.

    Pathwayi

    GO - Molecular functioni

    1. sialyltransferase activity Source: MGI

    GO - Biological processi

    1. carbohydrate biosynthetic process Source: MGI
    2. ganglioside biosynthetic process Source: BHF-UCL
    3. glycolipid biosynthetic process Source: MGI
    4. glycoprotein metabolic process Source: BHF-UCL
    5. oligosaccharide metabolic process Source: BHF-UCL
    6. protein O-linked glycosylation Source: MGI
    7. sialylation Source: GOC

    Keywords - Molecular functioni

    Glycosyltransferase, Transferase

    Enzyme and pathway databases

    ReactomeiREACT_198565. N-Glycan antennae elongation.
    UniPathwayiUPA00378.

    Protein family/group databases

    CAZyiGT29. Glycosyltransferase Family 29.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Alpha-2,8-sialyltransferase 8F (EC:2.4.99.-)
    Alternative name(s):
    Sialyltransferase 8F
    Short name:
    SIAT8-F
    Sialyltransferase St8Sia VI
    Short name:
    ST8SiaVI
    Gene namesi
    Name:St8sia6
    Synonyms:Siat8f
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 2

    Organism-specific databases

    MGIiMGI:2386797. St8sia6.

    Subcellular locationi

    GO - Cellular componenti

    1. integral component of Golgi membrane Source: InterPro

    Keywords - Cellular componenti

    Golgi apparatus, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 398398Alpha-2,8-sialyltransferase 8FPRO_0000149300Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi66 – 661N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi93 – 931N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi151 – 1511N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi186 ↔ 335By similarity
    Glycosylationi196 – 1961N-linked (GlcNAc...)Sequence Analysis

    Keywords - PTMi

    Disulfide bond, Glycoprotein

    Proteomic databases

    PaxDbiQ8K4T1.
    PRIDEiQ8K4T1.

    PTM databases

    PhosphoSiteiQ8K4T1.

    Expressioni

    Gene expression databases

    ArrayExpressiQ8K4T1.
    BgeeiQ8K4T1.
    GenevestigatoriQ8K4T1.

    Structurei

    3D structure databases

    ProteinModelPortaliQ8K4T1.
    SMRiQ8K4T1. Positions 182-358.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 33CytoplasmicSequence Analysis
    Topological domaini25 – 398374LumenalSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei4 – 2421Helical; Signal-anchor for type II membrane proteinSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the glycosyltransferase 29 family.Curated

    Keywords - Domaini

    Signal-anchor, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG323775.
    GeneTreeiENSGT00550000074407.
    HOVERGENiHBG106106.
    InParanoidiQ8K4T1.
    KOiK06615.
    OMAiNMSYEVE.
    OrthoDBiEOG7SV0V9.
    TreeFamiTF323961.

    Family and domain databases

    InterProiIPR001675. Glyco_trans_29.
    IPR012163. Sialyl_trans.
    [Graphical view]
    PfamiPF00777. Glyco_transf_29. 1 hit.
    [Graphical view]
    PIRSFiPIRSF005557. Sialyl_trans. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q8K4T1-1 [UniParc]FASTAAdd to Basket

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    MRSGGTLFAL IGSLMLLLLL RMLWCPADAP ARSRLLMEGS REDTSGTSAA    50
    LKTLWSPTTP VPRTRNSTYL DEKTTQITEK CKDLQYSLNS LSNKTRRYSE 100
    DDYLQTITNI QRCPWNRQAE EYDNFRAKLA SCCDAIQDFV VSQNNTPVGT 150
    NMSYEVESKK HIPIRENIFH MFPVSQPFVD YPYNQCAVVG NGGILNKSLC 200
    GAEIDKSDFV FRCNLPPITG SASKDVGSKT NLVTVNPSII TLKYQNLKEK 250
    KAQFLEDIST YGDAFLLLPA FSYRANTGIS FKVYQTLKES KMRQKVLFFH 300
    PRYLRHLALF WRTKGVTAYR LSTGLMIASV AVELCENVKL YGFWPFSKTI 350
    EDTPLSHHYY DNMLPKHGFH QMPKEYSQML QLHMRGILKL QFSKCETA 398
    Length:398
    Mass (Da):45,428
    Last modified:October 1, 2002 - v1
    Checksum:iA73A7A29640D4917
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB059554 mRNA. Translation: BAC01265.1.
    CCDSiCCDS15697.1.
    RefSeqiNP_665837.1. NM_145838.1.
    UniGeneiMm.330004.
    Mm.393036.

    Genome annotation databases

    EnsembliENSMUST00000003509; ENSMUSP00000003509; ENSMUSG00000003418.
    GeneIDi241230.
    KEGGimmu:241230.
    UCSCiuc008ikc.2. mouse.

    Cross-referencesi

    Web resourcesi

    Functional Glycomics Gateway - GTase

    ST8Sia VI

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB059554 mRNA. Translation: BAC01265.1 .
    CCDSi CCDS15697.1.
    RefSeqi NP_665837.1. NM_145838.1.
    UniGenei Mm.330004.
    Mm.393036.

    3D structure databases

    ProteinModelPortali Q8K4T1.
    SMRi Q8K4T1. Positions 182-358.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    CAZyi GT29. Glycosyltransferase Family 29.

    PTM databases

    PhosphoSitei Q8K4T1.

    Proteomic databases

    PaxDbi Q8K4T1.
    PRIDEi Q8K4T1.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000003509 ; ENSMUSP00000003509 ; ENSMUSG00000003418 .
    GeneIDi 241230.
    KEGGi mmu:241230.
    UCSCi uc008ikc.2. mouse.

    Organism-specific databases

    CTDi 338596.
    MGIi MGI:2386797. St8sia6.

    Phylogenomic databases

    eggNOGi NOG323775.
    GeneTreei ENSGT00550000074407.
    HOVERGENi HBG106106.
    InParanoidi Q8K4T1.
    KOi K06615.
    OMAi NMSYEVE.
    OrthoDBi EOG7SV0V9.
    TreeFami TF323961.

    Enzyme and pathway databases

    UniPathwayi UPA00378 .
    Reactomei REACT_198565. N-Glycan antennae elongation.

    Miscellaneous databases

    NextBioi 384941.
    PROi Q8K4T1.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q8K4T1.
    Bgeei Q8K4T1.
    Genevestigatori Q8K4T1.

    Family and domain databases

    InterProi IPR001675. Glyco_trans_29.
    IPR012163. Sialyl_trans.
    [Graphical view ]
    Pfami PF00777. Glyco_transf_29. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF005557. Sialyl_trans. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Molecular cloning and expression of a sixth type of alpha 2,8-sialyltransferase (ST8Sia VI) that sialylates O-glycans."
      Takashima S., Ishida H.K., Inazu T., Ando T., Ishida H., Kiso M., Tsuji S., Tsujimoto M.
      J. Biol. Chem. 277:24030-24038(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], CHARACTERIZATION.

    Entry informationi

    Entry nameiSIA8F_MOUSE
    AccessioniPrimary (citable) accession number: Q8K4T1
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: June 7, 2004
    Last sequence update: October 1, 2002
    Last modified: October 1, 2014
    This is version 92 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3