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Protein

Alpha-2,8-sialyltransferase 8F

Gene

St8sia6

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Prefers O-glycans to N-glycans or glycolipids as acceptor substrates. The minimal acceptor substrate is the NeuAc-alpha-2,36-Gal sequence at the non-reducing end of their carbohydrate groups.

Pathwayi: protein glycosylation

This protein is involved in the pathway protein glycosylation, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein glycosylation and in Protein modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei214SubstrateBy similarity1
Active sitei370Proton donor/acceptorBy similarity1

GO - Molecular functioni

  • sialyltransferase activity Source: MGI

GO - Biological processi

  • carbohydrate biosynthetic process Source: MGI
  • ganglioside biosynthetic process Source: BHF-UCL
  • glycolipid biosynthetic process Source: MGI
  • glycoprotein metabolic process Source: BHF-UCL
  • oligosaccharide metabolic process Source: BHF-UCL
  • protein O-linked glycosylation Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Enzyme and pathway databases

BRENDAi2.4.99.8. 3474.
ReactomeiR-MMU-975577. N-Glycan antennae elongation.
UniPathwayiUPA00378.

Protein family/group databases

CAZyiGT29. Glycosyltransferase Family 29.

Chemistry databases

SwissLipidsiSLP:000001419.

Names & Taxonomyi

Protein namesi
Recommended name:
Alpha-2,8-sialyltransferase 8F (EC:2.4.99.-)
Alternative name(s):
Sialyltransferase 8F
Short name:
SIAT8-F
Sialyltransferase St8Sia VI
Short name:
ST8SiaVI
Gene namesi
Name:St8sia6
Synonyms:Siat8f
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 2

Organism-specific databases

MGIiMGI:2386797. St8sia6.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 3CytoplasmicSequence analysis3
Transmembranei4 – 24Helical; Signal-anchor for type II membrane proteinSequence analysisAdd BLAST21
Topological domaini25 – 398LumenalSequence analysisAdd BLAST374

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001493001 – 398Alpha-2,8-sialyltransferase 8FAdd BLAST398

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi66N-linked (GlcNAc...)Sequence analysis1
Glycosylationi93N-linked (GlcNAc...)Sequence analysis1
Glycosylationi151N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi186 ↔ 335By similarity
Glycosylationi196N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi200 ↔ 395By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiQ8K4T1.
PRIDEiQ8K4T1.

PTM databases

PhosphoSitePlusiQ8K4T1.

Expressioni

Gene expression databases

BgeeiENSMUSG00000003418.
ExpressionAtlasiQ8K4T1. baseline and differential.
GenevisibleiQ8K4T1. MM.

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000003509.

Structurei

3D structure databases

ProteinModelPortaliQ8K4T1.
SMRiQ8K4T1.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni236 – 238Substrate bindingBy similarity3
Regioni322 – 324Substrate bindingBy similarity3

Sequence similaritiesi

Belongs to the glycosyltransferase 29 family.Curated

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG2692. Eukaryota.
ENOG410XT8P. LUCA.
GeneTreeiENSGT00550000074407.
HOVERGENiHBG106106.
InParanoidiQ8K4T1.
KOiK06615.
OMAiNMSYEVE.
OrthoDBiEOG091G0CER.
TreeFamiTF323961.

Family and domain databases

InterProiIPR001675. Glyco_trans_29.
IPR012163. Sialyl_trans.
[Graphical view]
PfamiPF00777. Glyco_transf_29. 1 hit.
[Graphical view]
PIRSFiPIRSF005557. Sialyl_trans. 1 hit.

Sequencei

Sequence statusi: Complete.

Q8K4T1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRSGGTLFAL IGSLMLLLLL RMLWCPADAP ARSRLLMEGS REDTSGTSAA
60 70 80 90 100
LKTLWSPTTP VPRTRNSTYL DEKTTQITEK CKDLQYSLNS LSNKTRRYSE
110 120 130 140 150
DDYLQTITNI QRCPWNRQAE EYDNFRAKLA SCCDAIQDFV VSQNNTPVGT
160 170 180 190 200
NMSYEVESKK HIPIRENIFH MFPVSQPFVD YPYNQCAVVG NGGILNKSLC
210 220 230 240 250
GAEIDKSDFV FRCNLPPITG SASKDVGSKT NLVTVNPSII TLKYQNLKEK
260 270 280 290 300
KAQFLEDIST YGDAFLLLPA FSYRANTGIS FKVYQTLKES KMRQKVLFFH
310 320 330 340 350
PRYLRHLALF WRTKGVTAYR LSTGLMIASV AVELCENVKL YGFWPFSKTI
360 370 380 390
EDTPLSHHYY DNMLPKHGFH QMPKEYSQML QLHMRGILKL QFSKCETA
Length:398
Mass (Da):45,428
Last modified:October 1, 2002 - v1
Checksum:iA73A7A29640D4917
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB059554 mRNA. Translation: BAC01265.1.
CCDSiCCDS15697.1.
RefSeqiNP_665837.1. NM_145838.1.
UniGeneiMm.330004.
Mm.393036.

Genome annotation databases

EnsembliENSMUST00000003509; ENSMUSP00000003509; ENSMUSG00000003418.
GeneIDi241230.
KEGGimmu:241230.
UCSCiuc008ikc.2. mouse.

Cross-referencesi

Web resourcesi

Functional Glycomics Gateway - GTase

ST8Sia VI

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB059554 mRNA. Translation: BAC01265.1.
CCDSiCCDS15697.1.
RefSeqiNP_665837.1. NM_145838.1.
UniGeneiMm.330004.
Mm.393036.

3D structure databases

ProteinModelPortaliQ8K4T1.
SMRiQ8K4T1.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000003509.

Chemistry databases

SwissLipidsiSLP:000001419.

Protein family/group databases

CAZyiGT29. Glycosyltransferase Family 29.

PTM databases

PhosphoSitePlusiQ8K4T1.

Proteomic databases

PaxDbiQ8K4T1.
PRIDEiQ8K4T1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000003509; ENSMUSP00000003509; ENSMUSG00000003418.
GeneIDi241230.
KEGGimmu:241230.
UCSCiuc008ikc.2. mouse.

Organism-specific databases

CTDi338596.
MGIiMGI:2386797. St8sia6.

Phylogenomic databases

eggNOGiKOG2692. Eukaryota.
ENOG410XT8P. LUCA.
GeneTreeiENSGT00550000074407.
HOVERGENiHBG106106.
InParanoidiQ8K4T1.
KOiK06615.
OMAiNMSYEVE.
OrthoDBiEOG091G0CER.
TreeFamiTF323961.

Enzyme and pathway databases

UniPathwayiUPA00378.
BRENDAi2.4.99.8. 3474.
ReactomeiR-MMU-975577. N-Glycan antennae elongation.

Miscellaneous databases

PROiQ8K4T1.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000003418.
ExpressionAtlasiQ8K4T1. baseline and differential.
GenevisibleiQ8K4T1. MM.

Family and domain databases

InterProiIPR001675. Glyco_trans_29.
IPR012163. Sialyl_trans.
[Graphical view]
PfamiPF00777. Glyco_transf_29. 1 hit.
[Graphical view]
PIRSFiPIRSF005557. Sialyl_trans. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiSIA8F_MOUSE
AccessioniPrimary (citable) accession number: Q8K4T1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 7, 2004
Last sequence update: October 1, 2002
Last modified: November 2, 2016
This is version 106 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.