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Protein

Alpha-2,8-sialyltransferase 8F

Gene

St8sia6

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Prefers O-glycans to N-glycans or glycolipids as acceptor substrates. The minimal acceptor substrate is the NeuAc-alpha-2,36-Gal sequence at the non-reducing end of their carbohydrate groups.

Pathwayi: protein glycosylation

This protein is involved in the pathway protein glycosylation, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein glycosylation and in Protein modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei214SubstrateBy similarity1
Active sitei370Proton donor/acceptorBy similarity1

GO - Molecular functioni

  • sialyltransferase activity Source: MGI

GO - Biological processi

  • carbohydrate biosynthetic process Source: MGI
  • ganglioside biosynthetic process Source: BHF-UCL
  • glycolipid biosynthetic process Source: MGI
  • glycoprotein metabolic process Source: BHF-UCL
  • oligosaccharide metabolic process Source: BHF-UCL
  • protein O-linked glycosylation Source: MGI

Keywordsi

Molecular functionGlycosyltransferase, Transferase

Enzyme and pathway databases

BRENDAi2.4.99.8 3474
ReactomeiR-MMU-4085001 Sialic acid metabolism
R-MMU-975577 N-Glycan antennae elongation
UniPathwayiUPA00378

Protein family/group databases

CAZyiGT29 Glycosyltransferase Family 29

Chemistry databases

SwissLipidsiSLP:000001419

Names & Taxonomyi

Protein namesi
Recommended name:
Alpha-2,8-sialyltransferase 8F (EC:2.4.99.-)
Alternative name(s):
Sialyltransferase 8F
Short name:
SIAT8-F
Sialyltransferase St8Sia VI
Short name:
ST8SiaVI
Gene namesi
Name:St8sia6
Synonyms:Siat8f
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 2

Organism-specific databases

MGIiMGI:2386797 St8sia6

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 3CytoplasmicSequence analysis3
Transmembranei4 – 24Helical; Signal-anchor for type II membrane proteinSequence analysisAdd BLAST21
Topological domaini25 – 398LumenalSequence analysisAdd BLAST374

Keywords - Cellular componenti

Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001493001 – 398Alpha-2,8-sialyltransferase 8FAdd BLAST398

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi66N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi93N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi151N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi186 ↔ 335By similarity
Glycosylationi196N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi200 ↔ 395By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiQ8K4T1
PRIDEiQ8K4T1

PTM databases

PhosphoSitePlusiQ8K4T1

Expressioni

Gene expression databases

BgeeiENSMUSG00000003418
ExpressionAtlasiQ8K4T1 baseline and differential
GenevisibleiQ8K4T1 MM

Interactioni

Protein-protein interaction databases

BioGridi232295, 1 interactor
STRINGi10090.ENSMUSP00000003509

Structurei

3D structure databases

ProteinModelPortaliQ8K4T1
SMRiQ8K4T1
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni236 – 238Substrate bindingBy similarity3
Regioni322 – 324Substrate bindingBy similarity3

Sequence similaritiesi

Belongs to the glycosyltransferase 29 family.Curated

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG2692 Eukaryota
ENOG410XT8P LUCA
GeneTreeiENSGT00550000074407
HOVERGENiHBG106106
InParanoidiQ8K4T1
KOiK06615
OMAiFHPKYLK
OrthoDBiEOG091G0CER
TreeFamiTF323961

Family and domain databases

Gene3Di3.90.1480.20, 1 hit
InterProiView protein in InterPro
IPR001675 Glyco_trans_29
IPR038578 GT29-like_sf
IPR012163 Sialyl_trans
PfamiView protein in Pfam
PF00777 Glyco_transf_29, 1 hit
PIRSFiPIRSF005557 Sialyl_trans, 1 hit

Sequencei

Sequence statusi: Complete.

Q8K4T1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRSGGTLFAL IGSLMLLLLL RMLWCPADAP ARSRLLMEGS REDTSGTSAA
60 70 80 90 100
LKTLWSPTTP VPRTRNSTYL DEKTTQITEK CKDLQYSLNS LSNKTRRYSE
110 120 130 140 150
DDYLQTITNI QRCPWNRQAE EYDNFRAKLA SCCDAIQDFV VSQNNTPVGT
160 170 180 190 200
NMSYEVESKK HIPIRENIFH MFPVSQPFVD YPYNQCAVVG NGGILNKSLC
210 220 230 240 250
GAEIDKSDFV FRCNLPPITG SASKDVGSKT NLVTVNPSII TLKYQNLKEK
260 270 280 290 300
KAQFLEDIST YGDAFLLLPA FSYRANTGIS FKVYQTLKES KMRQKVLFFH
310 320 330 340 350
PRYLRHLALF WRTKGVTAYR LSTGLMIASV AVELCENVKL YGFWPFSKTI
360 370 380 390
EDTPLSHHYY DNMLPKHGFH QMPKEYSQML QLHMRGILKL QFSKCETA
Length:398
Mass (Da):45,428
Last modified:October 1, 2002 - v1
Checksum:iA73A7A29640D4917
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB059554 mRNA Translation: BAC01265.1
CCDSiCCDS15697.1
RefSeqiNP_665837.1, NM_145838.1
UniGeneiMm.330004
Mm.393036

Genome annotation databases

EnsembliENSMUST00000003509; ENSMUSP00000003509; ENSMUSG00000003418
GeneIDi241230
KEGGimmu:241230
UCSCiuc008ikc.2 mouse

Similar proteinsi

Entry informationi

Entry nameiSIA8F_MOUSE
AccessioniPrimary (citable) accession number: Q8K4T1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 7, 2004
Last sequence update: October 1, 2002
Last modified: March 28, 2018
This is version 114 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

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