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Q8K4R9

- DLGP5_MOUSE

UniProt

Q8K4R9 - DLGP5_MOUSE

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Protein

Disks large-associated protein 5

Gene

Dlgap5

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

Potential cell cycle regulator that may play a role in carcinogenesis of cancer cells. Mitotic phosphoprotein regulated by the ubiquitin-proteasome pathway. Key regulator of adherens junction integrity and differentiation that may be involved in CDH1-mediated adhesion and signaling in epithelial cells (By similarity).By similarity

GO - Molecular functioni

  1. phosphoprotein phosphatase activity Source: UniProtKB

GO - Biological processi

  1. cell-cell signaling Source: InterPro
  2. cell cycle Source: UniProtKB-KW
  3. dephosphorylation Source: GOC
Complete GO annotation...

Keywords - Biological processi

Cell cycle

Names & Taxonomyi

Protein namesi
Recommended name:
Disks large-associated protein 5
Short name:
DAP-5
Alternative name(s):
Discs large homolog 7
Disks large-associated protein DLG7
Hepatoma up-regulated protein homolog
Short name:
HURP
Gene namesi
Name:Dlgap5
Synonyms:Dlg7, Kiaa0008
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 14

Organism-specific databases

MGIiMGI:2183453. Dlgap5.

Subcellular locationi

Nucleus By similarity. Cytoplasm By similarity. Cytoplasmcytoskeletonspindle By similarity
Note: Localizes to the spindle in mitotic cells. Colocalizes with CDH1 at sites of cell-cell contact in intestinal epithelial cells (By similarity).By similarity

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-KW
  2. nucleus Source: UniProtKB
  3. spindle pole centrosome Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 808808Disks large-associated protein 5PRO_0000174300Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei66 – 661PhosphoserineBy similarity
Modified residuei328 – 3281Phosphoserine1 Publication
Modified residuei337 – 3371PhosphothreonineBy similarity
Modified residuei386 – 3861PhosphothreonineBy similarity
Modified residuei598 – 5981PhosphoserineBy similarity
Modified residuei607 – 6071Phosphoserine; by AURKABy similarity
Modified residuei617 – 6171PhosphothreonineBy similarity
Modified residuei620 – 6201PhosphoserineBy similarity
Modified residuei728 – 7281PhosphothreonineBy similarity
Modified residuei797 – 7971Phosphoserine; by AURKABy similarity
Modified residuei806 – 8061PhosphoserineBy similarity

Post-translational modificationi

Ubiquitinated, leading to its degradation.By similarity
Decreased phosphorylation levels are associated with the differentiation of intestinal epithelial cells.By similarity

Keywords - PTMi

Phosphoprotein, Ubl conjugation

Proteomic databases

MaxQBiQ8K4R9.
PaxDbiQ8K4R9.
PRIDEiQ8K4R9.

PTM databases

PhosphoSiteiQ8K4R9.

Expressioni

Tissue specificityi

Expressed at low levels in normal resting liver. Up-regulated in regenerating liver after partial hepatectomy.1 Publication

Gene expression databases

BgeeiQ8K4R9.
GenevestigatoriQ8K4R9.

Interactioni

Subunit structurei

Interacts with CDC2. Interacts with the C-terminal proline-rich region of FBXO7. Recruited by FBXO7 to a SCF (SKP1-CUL1-F-box) protein complex in a CDC2/Cyclin B-phosphorylation dependent manner. Interacts with CDH1 (By similarity).By similarity

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000040416.

Family & Domainsi

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili88 – 11932Sequence AnalysisAdd
BLAST

Sequence similaritiesi

Belongs to the SAPAP family.Curated

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiNOG270787.
GeneTreeiENSGT00550000074473.
HOGENOMiHOG000054220.
HOVERGENiHBG062172.
InParanoidiQ8K4R9.
KOiK16804.
OrthoDBiEOG75QR37.
PhylomeDBiQ8K4R9.
TreeFamiTF321382.

Family and domain databases

InterProiIPR005026. GKAP.
[Graphical view]
PfamiPF03359. GKAP. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. Align

Isoform 11 Publication (identifier: Q8K4R9-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MLVSRFASRF RKDSSTEMVR TNLAHRKSLS QKENRHRVYE RNRHFGLKDV
60 70 80 90 100
NIPLEGRELG NIHETSQDLS PEKASSKTRS VKMVLSDQRK QLLQKYKEEK
110 120 130 140 150
QLQKLKEQRE KAKRGVFKVG LYRPAAPGFL VTDQRGAKAE PEKAFPHTGR
160 170 180 190 200
ITRSKTKEYM EQTKIGSRNV PKATQSDQRQ TSEKQPLDRE RKVMQPVLFT
210 220 230 240 250
SGKGTESAAT QRAKLMARTV SSTTRKPVTR ATNEKGSERM RPSGGRPAKK
260 270 280 290 300
PEGKPDKVIP SKVERDEKHL DSQTRETSEM GPLGVFREVE SLPATAPAQG
310 320 330 340 350
KERKSFAPKH CVFQPPCGLK SYQVAPLSPR SANAFLTPNC DWNQLRPEVF
360 370 380 390 400
STTTQDKANE ILVQQGLESL TDRSKEHVLN QKGASTSDSN HASVKGVPCS
410 420 430 440 450
EGSEGQTSQP PHDVPYFRKI LQSETDRLTS HCLEWEGKLD LDISDEAKGL
460 470 480 490 500
IRTTVGQTRL LIKERFRQFE GLVDNCEYKR GEKETTCTDL DGFWDMVSFQ
510 520 530 540 550
VDDVNQKFNN LIKLEASGWK DSNNPSKKVL RKKIVPGRTS KAKQDDDGRA
560 570 580 590 600
AARSRLAAIK NAMKGRPQQE VQAHAAAPET TKEVDKIVFD AGFFRIESPV
610 620 630 640 650
KSFSVLSSER RSQRFGTPLS ASKVVPEGRA AGDLLRQKMP LKKPDPQSSK
660 670 680 690 700
SEHVDRTFSD GLESRCHVED TPCPGEQDSS DIEHDVNKIN VKMDCFSVET
710 720 730 740 750
NLPLPAGDAN TNQKEAISAV EGASTAVTSQ DLLMSNPETN TSSQSNTSQE
760 770 780 790 800
EAEASQSVLL HKSLTSECHL LEPPGLSCTS PCTREETRQP DRSRQFSFGG

DLILFSPL
Length:808
Mass (Da):90,196
Last modified:September 13, 2005 - v2
Checksum:i9F9B0EBA3CCF705C
GO
Isoform 2Curated (identifier: Q8K4R9-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     758-758: V → G
     759-808: Missing.

Note: No experimental confirmation available.Curated

Show »
Length:758
Mass (Da):84,571
Checksum:iB2EE5A031E04019F
GO
Isoform 3Curated (identifier: Q8K4R9-3) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     606-627: LSSERRSQRFGTPLSASKVVPE → CSFAETEERCLGQLGAGVNGGE
     628-808: Missing.

Note: No experimental confirmation available.Curated

Show »
Length:627
Mass (Da):70,380
Checksum:i53C9D9F7F78EBD00
GO

Sequence cautioni

The sequence BAC97842.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti165 – 1651I → M in BAC97842. (PubMed:14621295)Curated
Sequence conflicti282 – 2821P → L in BAB97377. (PubMed:12527899)Curated
Sequence conflicti359 – 3591N → D in AAH43924. (PubMed:15489334)Curated
Sequence conflicti402 – 4021G → A in BAC29405. (PubMed:16141072)Curated
Sequence conflicti402 – 4021G → A in BAC39560. (PubMed:16141072)Curated
Sequence conflicti402 – 4021G → A in AAH58087. (PubMed:15489334)Curated
Sequence conflicti433 – 4331L → Q in BAC29405. (PubMed:16141072)Curated
Sequence conflicti433 – 4331L → Q in BAC39560. (PubMed:16141072)Curated
Sequence conflicti433 – 4331L → Q in AAH58087. (PubMed:15489334)Curated
Sequence conflicti439 – 4391L → Q in BAC29405. (PubMed:16141072)Curated
Sequence conflicti444 – 4441S → P in BAC29405. (PubMed:16141072)Curated
Sequence conflicti444 – 4441S → P in BAC39560. (PubMed:16141072)Curated
Sequence conflicti444 – 4441S → P in AAH58087. (PubMed:15489334)Curated
Sequence conflicti546 – 5461D → E in BAC97842. (PubMed:14621295)Curated
Sequence conflicti546 – 5461D → E in AAH43924. (PubMed:15489334)Curated
Sequence conflicti580 – 5801T → N in BAC29405. (PubMed:16141072)Curated
Sequence conflicti580 – 5801T → N in BAC39560. (PubMed:16141072)Curated
Sequence conflicti580 – 5801T → N in AAH58087. (PubMed:15489334)Curated
Sequence conflicti639 – 6391M → N in BAC97842. (PubMed:14621295)Curated
Sequence conflicti639 – 6391M → N in AAH43924. (PubMed:15489334)Curated
Sequence conflicti725 – 7251T → S in BAC39560. (PubMed:16141072)Curated
Sequence conflicti725 – 7251T → S in AAH58087. (PubMed:15489334)Curated
Sequence conflicti808 – 8081L → LRP in AAH43924. (PubMed:15489334)Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei606 – 62722LSSER…KVVPE → CSFAETEERCLGQLGAGVNG GE in isoform 3. 1 PublicationVSP_051825Add
BLAST
Alternative sequencei628 – 808181Missing in isoform 3. 1 PublicationVSP_051826Add
BLAST
Alternative sequencei758 – 7581V → G in isoform 2. 1 PublicationVSP_051827
Alternative sequencei759 – 80850Missing in isoform 2. 1 PublicationVSP_051828Add
BLAST

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB076696 mRNA. Translation: BAB97377.1.
AK036384 mRNA. Translation: BAC29405.1.
AK085882 mRNA. Translation: BAC39560.1.
AK129032 mRNA. Translation: BAC97842.1. Different initiation.
BC043924 mRNA. Translation: AAH43924.1.
BC058087 mRNA. Translation: AAH58087.1.
CCDSiCCDS26987.1. [Q8K4R9-1]
RefSeqiNP_653136.2. NM_144553.2.
UniGeneiMm.240543.

Genome annotation databases

EnsembliENSMUST00000043296; ENSMUSP00000040416; ENSMUSG00000037544.
GeneIDi218977.
KEGGimmu:218977.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB076696 mRNA. Translation: BAB97377.1 .
AK036384 mRNA. Translation: BAC29405.1 .
AK085882 mRNA. Translation: BAC39560.1 .
AK129032 mRNA. Translation: BAC97842.1 . Different initiation.
BC043924 mRNA. Translation: AAH43924.1 .
BC058087 mRNA. Translation: AAH58087.1 .
CCDSi CCDS26987.1. [Q8K4R9-1 ]
RefSeqi NP_653136.2. NM_144553.2.
UniGenei Mm.240543.

3D structure databases

ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 10090.ENSMUSP00000040416.

PTM databases

PhosphoSitei Q8K4R9.

Proteomic databases

MaxQBi Q8K4R9.
PaxDbi Q8K4R9.
PRIDEi Q8K4R9.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000043296 ; ENSMUSP00000040416 ; ENSMUSG00000037544 .
GeneIDi 218977.
KEGGi mmu:218977.

Organism-specific databases

CTDi 9787.
MGIi MGI:2183453. Dlgap5.
Rougei Search...

Phylogenomic databases

eggNOGi NOG270787.
GeneTreei ENSGT00550000074473.
HOGENOMi HOG000054220.
HOVERGENi HBG062172.
InParanoidi Q8K4R9.
KOi K16804.
OrthoDBi EOG75QR37.
PhylomeDBi Q8K4R9.
TreeFami TF321382.

Miscellaneous databases

NextBioi 376509.
PROi Q8K4R9.
SOURCEi Search...

Gene expression databases

Bgeei Q8K4R9.
Genevestigatori Q8K4R9.

Family and domain databases

InterProi IPR005026. GKAP.
[Graphical view ]
Pfami PF03359. GKAP. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Identification of a novel cell cycle regulated gene, HURP, overexpressed in human hepatocellular carcinoma."
    Tsou A.-P., Yang C.-W., Huang C.-Y.F., Yu R.C.-T., Lee Y.-C.G., Chang C.-W., Chen B.-R., Chung Y.-F., Fann M.-J., Chi C.-W., Chiu J.-H., Chou C.-K.
    Oncogene 22:298-307(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), TISSUE SPECIFICITY.
  2. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 3).
    Strain: C57BL/6J.
    Tissue: Bone and Heart.
  3. "Prediction of the coding sequences of mouse homologues of KIAA gene: III. The complete nucleotide sequences of 500 mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries."
    Okazaki N., Kikuno R., Ohara R., Inamoto S., Koseki H., Hiraoka S., Saga Y., Nagase T., Ohara O., Koga H.
    DNA Res. 10:167-180(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    Tissue: Embryonic tailImported.
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Strain: C57BL/6Imported.
    Tissue: BrainImported and Mammary glandImported.
  5. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-328, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Liver.

Entry informationi

Entry nameiDLGP5_MOUSE
AccessioniPrimary (citable) accession number: Q8K4R9
Secondary accession number(s): Q6ZQL3
, Q80VS7, Q8BUC8, Q8BZ79
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 13, 2005
Last sequence update: September 13, 2005
Last modified: October 29, 2014
This is version 88 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3