Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

Q8K4R9 (DLGP5_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 86. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Disks large-associated protein 5

Short name=DAP-5
Alternative name(s):
Discs large homolog 7
Disks large-associated protein DLG7
Hepatoma up-regulated protein homolog
Short name=HURP
Gene names
Name:Dlgap5
Synonyms:Dlg7, Kiaa0008
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length808 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Potential cell cycle regulator that may play a role in carcinogenesis of cancer cells. Mitotic phosphoprotein regulated by the ubiquitin-proteasome pathway. Key regulator of adherens junction integrity and differentiation that may be involved in CDH1-mediated adhesion and signaling in epithelial cells By similarity. UniProtKB Q15398

Subunit structure

Interacts with CDC2. Interacts with the C-terminal proline-rich region of FBXO7. Recruited by FBXO7 to a SCF (SKP1-CUL1-F-box) protein complex in a CDC2/Cyclin B-phosphorylation dependent manner. Interacts with CDH1 By similarity. UniProtKB Q15398

Subcellular location

Nucleus By similarity. Cytoplasm By similarity. Cytoplasmcytoskeletonspindle By similarity. Note: Localizes to the spindle in mitotic cells. Colocalizes with CDH1 at sites of cell-cell contact in intestinal epithelial cells By similarity.

Tissue specificity

Expressed at low levels in normal resting liver. Up-regulated in regenerating liver after partial hepatectomy. Ref.1

Post-translational modification

Ubiquitinated, leading to its degradation By similarity.

Decreased phosphorylation levels are associated with the differentiation of intestinal epithelial cells By similarity. UniProtKB Q15398

Sequence similarities

Belongs to the SAPAP family.

Sequence caution

The sequence BAC97842.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 Ref.1 (identifier: Q8K4R9-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q8K4R9-2)

The sequence of this isoform differs from the canonical sequence as follows:
     758-758: V → G
     759-808: Missing.
Note: No experimental confirmation available.
Isoform 3 (identifier: Q8K4R9-3)

The sequence of this isoform differs from the canonical sequence as follows:
     606-627: LSSERRSQRFGTPLSASKVVPE → CSFAETEERCLGQLGAGVNGGE
     628-808: Missing.
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 808808Disks large-associated protein 5
PRO_0000174300

Regions

Coiled coil88 – 11932 Potential

Amino acid modifications

Modified residue661Phosphoserine By similarity UniProtKB Q15398
Modified residue3281Phosphoserine Ref.5 UniProtKB Q15398
Modified residue3371Phosphothreonine By similarity
Modified residue3861Phosphothreonine By similarity UniProtKB Q15398
Modified residue5981Phosphoserine By similarity UniProtKB Q15398
Modified residue6071Phosphoserine; by AURKA By similarity
Modified residue6171Phosphothreonine By similarity UniProtKB Q15398
Modified residue6201Phosphoserine By similarity UniProtKB Q15398
Modified residue7281Phosphothreonine By similarity UniProtKB Q15398
Modified residue7971Phosphoserine; by AURKA By similarity
Modified residue8061Phosphoserine By similarity UniProtKB Q15398

Natural variations

Alternative sequence606 – 62722LSSER…KVVPE → CSFAETEERCLGQLGAGVNG GE in isoform 3.
VSP_051825
Alternative sequence628 – 808181Missing in isoform 3.
VSP_051826
Alternative sequence7581V → G in isoform 2.
VSP_051827
Alternative sequence759 – 80850Missing in isoform 2.
VSP_051828

Experimental info

Sequence conflict1651I → M in BAC97842. Ref.3
Sequence conflict2821P → L in BAB97377. Ref.1
Sequence conflict3591N → D in AAH43924. Ref.4
Sequence conflict4021G → A in BAC29405. Ref.2
Sequence conflict4021G → A in BAC39560. Ref.2
Sequence conflict4021G → A in AAH58087. Ref.4
Sequence conflict4331L → Q in BAC29405. Ref.2
Sequence conflict4331L → Q in BAC39560. Ref.2
Sequence conflict4331L → Q in AAH58087. Ref.4
Sequence conflict4391L → Q in BAC29405. Ref.2
Sequence conflict4441S → P in BAC29405. Ref.2
Sequence conflict4441S → P in BAC39560. Ref.2
Sequence conflict4441S → P in AAH58087. Ref.4
Sequence conflict5461D → E in BAC97842. Ref.3
Sequence conflict5461D → E in AAH43924. Ref.4
Sequence conflict5801T → N in BAC29405. Ref.2
Sequence conflict5801T → N in BAC39560. Ref.2
Sequence conflict5801T → N in AAH58087. Ref.4
Sequence conflict6391M → N in BAC97842. Ref.3
Sequence conflict6391M → N in AAH43924. Ref.4
Sequence conflict7251T → S in BAC39560. Ref.2
Sequence conflict7251T → S in AAH58087. Ref.4
Sequence conflict8081L → LRP in AAH43924. Ref.4

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified September 13, 2005. Version 2.
Checksum: 9F9B0EBA3CCF705C

FASTA80890,196
        10         20         30         40         50         60 
MLVSRFASRF RKDSSTEMVR TNLAHRKSLS QKENRHRVYE RNRHFGLKDV NIPLEGRELG 

        70         80         90        100        110        120 
NIHETSQDLS PEKASSKTRS VKMVLSDQRK QLLQKYKEEK QLQKLKEQRE KAKRGVFKVG 

       130        140        150        160        170        180 
LYRPAAPGFL VTDQRGAKAE PEKAFPHTGR ITRSKTKEYM EQTKIGSRNV PKATQSDQRQ 

       190        200        210        220        230        240 
TSEKQPLDRE RKVMQPVLFT SGKGTESAAT QRAKLMARTV SSTTRKPVTR ATNEKGSERM 

       250        260        270        280        290        300 
RPSGGRPAKK PEGKPDKVIP SKVERDEKHL DSQTRETSEM GPLGVFREVE SLPATAPAQG 

       310        320        330        340        350        360 
KERKSFAPKH CVFQPPCGLK SYQVAPLSPR SANAFLTPNC DWNQLRPEVF STTTQDKANE 

       370        380        390        400        410        420 
ILVQQGLESL TDRSKEHVLN QKGASTSDSN HASVKGVPCS EGSEGQTSQP PHDVPYFRKI 

       430        440        450        460        470        480 
LQSETDRLTS HCLEWEGKLD LDISDEAKGL IRTTVGQTRL LIKERFRQFE GLVDNCEYKR 

       490        500        510        520        530        540 
GEKETTCTDL DGFWDMVSFQ VDDVNQKFNN LIKLEASGWK DSNNPSKKVL RKKIVPGRTS 

       550        560        570        580        590        600 
KAKQDDDGRA AARSRLAAIK NAMKGRPQQE VQAHAAAPET TKEVDKIVFD AGFFRIESPV 

       610        620        630        640        650        660 
KSFSVLSSER RSQRFGTPLS ASKVVPEGRA AGDLLRQKMP LKKPDPQSSK SEHVDRTFSD 

       670        680        690        700        710        720 
GLESRCHVED TPCPGEQDSS DIEHDVNKIN VKMDCFSVET NLPLPAGDAN TNQKEAISAV 

       730        740        750        760        770        780 
EGASTAVTSQ DLLMSNPETN TSSQSNTSQE EAEASQSVLL HKSLTSECHL LEPPGLSCTS 

       790        800 
PCTREETRQP DRSRQFSFGG DLILFSPL 

« Hide

Isoform 2 [UniParc].

Checksum: B2EE5A031E04019F
Show »

FASTA75884,571
Isoform 3 [UniParc].

Checksum: 53C9D9F7F78EBD00
Show »

FASTA62770,380

References

« Hide 'large scale' references
[1]"Identification of a novel cell cycle regulated gene, HURP, overexpressed in human hepatocellular carcinoma."
Tsou A.-P., Yang C.-W., Huang C.-Y.F., Yu R.C.-T., Lee Y.-C.G., Chang C.-W., Chen B.-R., Chung Y.-F., Fann M.-J., Chi C.-W., Chiu J.-H., Chou C.-K.
Oncogene 22:298-307(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), TISSUE SPECIFICITY.
[2]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 3).
Strain: C57BL/6J.
Tissue: Bone and Heart.
[3]"Prediction of the coding sequences of mouse homologues of KIAA gene: III. The complete nucleotide sequences of 500 mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries."
Okazaki N., Kikuno R., Ohara R., Inamoto S., Koseki H., Hiraoka S., Saga Y., Nagase T., Ohara O., Koga H.
DNA Res. 10:167-180(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
Tissue: Embryonic tail.
[4]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Strain: C57BL/6.
Tissue: Brain and Mammary gland.
[5]"Large-scale phosphorylation analysis of mouse liver."
Villen J., Beausoleil S.A., Gerber S.A., Gygi S.P.
Proc. Natl. Acad. Sci. U.S.A. 104:1488-1493(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-328, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Liver.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AB076696 mRNA. Translation: BAB97377.1.
AK036384 mRNA. Translation: BAC29405.1.
AK085882 mRNA. Translation: BAC39560.1.
AK129032 mRNA. Translation: BAC97842.1. Different initiation.
BC043924 mRNA. Translation: AAH43924.1.
BC058087 mRNA. Translation: AAH58087.1.
CCDSCCDS26987.1. [Q8K4R9-1]
RefSeqNP_653136.2. NM_144553.2.
UniGeneMm.240543.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING10090.ENSMUSP00000040416.

PTM databases

PhosphoSiteQ8K4R9.

Proteomic databases

PaxDbQ8K4R9.
PRIDEQ8K4R9.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000043296; ENSMUSP00000040416; ENSMUSG00000037544.
GeneID218977.
KEGGmmu:218977.

Organism-specific databases

CTD9787.
MGIMGI:2183453. Dlgap5.
RougeSearch...

Phylogenomic databases

eggNOGNOG270787.
GeneTreeENSGT00550000074473.
HOGENOMHOG000054220.
HOVERGENHBG062172.
InParanoidQ8K4R9.
KOK16804.
OrthoDBEOG75QR37.
PhylomeDBQ8K4R9.
TreeFamTF321382.

Gene expression databases

ArrayExpressQ8K4R9.
BgeeQ8K4R9.
GenevestigatorQ8K4R9.

Family and domain databases

InterProIPR005026. GKAP.
[Graphical view]
PfamPF03359. GKAP. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio376509.
PROQ8K4R9.
SOURCESearch...

Entry information

Entry nameDLGP5_MOUSE
AccessionPrimary (citable) accession number: Q8K4R9
Secondary accession number(s): Q6ZQL3 expand/collapse secondary AC list , Q80VS7, Q8BUC8, Q8BZ79
Entry history
Integrated into UniProtKB/Swiss-Prot: September 13, 2005
Last sequence update: September 13, 2005
Last modified: July 9, 2014
This is version 86 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot