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Q8K4R9

- DLGP5_MOUSE

UniProt

Q8K4R9 - DLGP5_MOUSE

Protein

Disks large-associated protein 5

Gene

Dlgap5

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 87 (01 Oct 2014)
      Sequence version 2 (13 Sep 2005)
      Previous versions | rss
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    Functioni

    Potential cell cycle regulator that may play a role in carcinogenesis of cancer cells. Mitotic phosphoprotein regulated by the ubiquitin-proteasome pathway. Key regulator of adherens junction integrity and differentiation that may be involved in CDH1-mediated adhesion and signaling in epithelial cells By similarity.By similarity

    GO - Molecular functioni

    1. phosphoprotein phosphatase activity Source: UniProtKB

    GO - Biological processi

    1. cell-cell signaling Source: InterPro
    2. dephosphorylation Source: GOC
    3. mitotic M phase Source: UniProtKB

    Keywords - Biological processi

    Cell cycle

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Disks large-associated protein 5
    Short name:
    DAP-5
    Alternative name(s):
    Discs large homolog 7
    Disks large-associated protein DLG7
    Hepatoma up-regulated protein homolog
    Short name:
    HURP
    Gene namesi
    Name:Dlgap5
    Synonyms:Dlg7, Kiaa0008
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 14

    Organism-specific databases

    MGIiMGI:2183453. Dlgap5.

    Subcellular locationi

    Nucleus By similarity. Cytoplasm By similarity. Cytoplasmcytoskeletonspindle By similarity
    Note: Localizes to the spindle in mitotic cells. Colocalizes with CDH1 at sites of cell-cell contact in intestinal epithelial cells By similarity.By similarity

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell
    2. nucleus Source: UniProtKB
    3. spindle pole centrosome Source: UniProtKB

    Keywords - Cellular componenti

    Cytoplasm, Cytoskeleton, Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 808808Disks large-associated protein 5PRO_0000174300Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei66 – 661PhosphoserineBy similarity
    Modified residuei328 – 3281Phosphoserine1 Publication
    Modified residuei337 – 3371PhosphothreonineBy similarity
    Modified residuei386 – 3861PhosphothreonineBy similarity
    Modified residuei598 – 5981PhosphoserineBy similarity
    Modified residuei607 – 6071Phosphoserine; by AURKABy similarity
    Modified residuei617 – 6171PhosphothreonineBy similarity
    Modified residuei620 – 6201PhosphoserineBy similarity
    Modified residuei728 – 7281PhosphothreonineBy similarity
    Modified residuei797 – 7971Phosphoserine; by AURKABy similarity
    Modified residuei806 – 8061PhosphoserineBy similarity

    Post-translational modificationi

    Ubiquitinated, leading to its degradation.By similarity
    Decreased phosphorylation levels are associated with the differentiation of intestinal epithelial cells.By similarity

    Keywords - PTMi

    Phosphoprotein, Ubl conjugation

    Proteomic databases

    PaxDbiQ8K4R9.
    PRIDEiQ8K4R9.

    PTM databases

    PhosphoSiteiQ8K4R9.

    Expressioni

    Tissue specificityi

    Expressed at low levels in normal resting liver. Up-regulated in regenerating liver after partial hepatectomy.1 Publication

    Gene expression databases

    ArrayExpressiQ8K4R9.
    BgeeiQ8K4R9.
    GenevestigatoriQ8K4R9.

    Interactioni

    Subunit structurei

    Interacts with CDC2. Interacts with the C-terminal proline-rich region of FBXO7. Recruited by FBXO7 to a SCF (SKP1-CUL1-F-box) protein complex in a CDC2/Cyclin B-phosphorylation dependent manner. Interacts with CDH1 By similarity.By similarity

    Protein-protein interaction databases

    STRINGi10090.ENSMUSP00000040416.

    Family & Domainsi

    Coiled coil

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Coiled coili88 – 11932Sequence AnalysisAdd
    BLAST

    Sequence similaritiesi

    Belongs to the SAPAP family.Curated

    Keywords - Domaini

    Coiled coil

    Phylogenomic databases

    eggNOGiNOG270787.
    GeneTreeiENSGT00550000074473.
    HOGENOMiHOG000054220.
    HOVERGENiHBG062172.
    InParanoidiQ8K4R9.
    KOiK16804.
    OrthoDBiEOG75QR37.
    PhylomeDBiQ8K4R9.
    TreeFamiTF321382.

    Family and domain databases

    InterProiIPR005026. GKAP.
    [Graphical view]
    PfamiPF03359. GKAP. 1 hit.
    [Graphical view]

    Sequences (3)i

    Sequence statusi: Complete.

    This entry describes 3 isoformsi produced by alternative splicing. Align

    Isoform 11 Publication (identifier: Q8K4R9-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MLVSRFASRF RKDSSTEMVR TNLAHRKSLS QKENRHRVYE RNRHFGLKDV    50
    NIPLEGRELG NIHETSQDLS PEKASSKTRS VKMVLSDQRK QLLQKYKEEK 100
    QLQKLKEQRE KAKRGVFKVG LYRPAAPGFL VTDQRGAKAE PEKAFPHTGR 150
    ITRSKTKEYM EQTKIGSRNV PKATQSDQRQ TSEKQPLDRE RKVMQPVLFT 200
    SGKGTESAAT QRAKLMARTV SSTTRKPVTR ATNEKGSERM RPSGGRPAKK 250
    PEGKPDKVIP SKVERDEKHL DSQTRETSEM GPLGVFREVE SLPATAPAQG 300
    KERKSFAPKH CVFQPPCGLK SYQVAPLSPR SANAFLTPNC DWNQLRPEVF 350
    STTTQDKANE ILVQQGLESL TDRSKEHVLN QKGASTSDSN HASVKGVPCS 400
    EGSEGQTSQP PHDVPYFRKI LQSETDRLTS HCLEWEGKLD LDISDEAKGL 450
    IRTTVGQTRL LIKERFRQFE GLVDNCEYKR GEKETTCTDL DGFWDMVSFQ 500
    VDDVNQKFNN LIKLEASGWK DSNNPSKKVL RKKIVPGRTS KAKQDDDGRA 550
    AARSRLAAIK NAMKGRPQQE VQAHAAAPET TKEVDKIVFD AGFFRIESPV 600
    KSFSVLSSER RSQRFGTPLS ASKVVPEGRA AGDLLRQKMP LKKPDPQSSK 650
    SEHVDRTFSD GLESRCHVED TPCPGEQDSS DIEHDVNKIN VKMDCFSVET 700
    NLPLPAGDAN TNQKEAISAV EGASTAVTSQ DLLMSNPETN TSSQSNTSQE 750
    EAEASQSVLL HKSLTSECHL LEPPGLSCTS PCTREETRQP DRSRQFSFGG 800
    DLILFSPL 808
    Length:808
    Mass (Da):90,196
    Last modified:September 13, 2005 - v2
    Checksum:i9F9B0EBA3CCF705C
    GO
    Isoform 2Curated (identifier: Q8K4R9-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         758-758: V → G
         759-808: Missing.

    Note: No experimental confirmation available.Curated

    Show »
    Length:758
    Mass (Da):84,571
    Checksum:iB2EE5A031E04019F
    GO
    Isoform 3Curated (identifier: Q8K4R9-3) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         606-627: LSSERRSQRFGTPLSASKVVPE → CSFAETEERCLGQLGAGVNGGE
         628-808: Missing.

    Note: No experimental confirmation available.Curated

    Show »
    Length:627
    Mass (Da):70,380
    Checksum:i53C9D9F7F78EBD00
    GO

    Sequence cautioni

    The sequence BAC97842.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti165 – 1651I → M in BAC97842. (PubMed:14621295)Curated
    Sequence conflicti282 – 2821P → L in BAB97377. (PubMed:12527899)Curated
    Sequence conflicti359 – 3591N → D in AAH43924. (PubMed:15489334)Curated
    Sequence conflicti402 – 4021G → A in BAC29405. (PubMed:16141072)Curated
    Sequence conflicti402 – 4021G → A in BAC39560. (PubMed:16141072)Curated
    Sequence conflicti402 – 4021G → A in AAH58087. (PubMed:15489334)Curated
    Sequence conflicti433 – 4331L → Q in BAC29405. (PubMed:16141072)Curated
    Sequence conflicti433 – 4331L → Q in BAC39560. (PubMed:16141072)Curated
    Sequence conflicti433 – 4331L → Q in AAH58087. (PubMed:15489334)Curated
    Sequence conflicti439 – 4391L → Q in BAC29405. (PubMed:16141072)Curated
    Sequence conflicti444 – 4441S → P in BAC29405. (PubMed:16141072)Curated
    Sequence conflicti444 – 4441S → P in BAC39560. (PubMed:16141072)Curated
    Sequence conflicti444 – 4441S → P in AAH58087. (PubMed:15489334)Curated
    Sequence conflicti546 – 5461D → E in BAC97842. (PubMed:14621295)Curated
    Sequence conflicti546 – 5461D → E in AAH43924. (PubMed:15489334)Curated
    Sequence conflicti580 – 5801T → N in BAC29405. (PubMed:16141072)Curated
    Sequence conflicti580 – 5801T → N in BAC39560. (PubMed:16141072)Curated
    Sequence conflicti580 – 5801T → N in AAH58087. (PubMed:15489334)Curated
    Sequence conflicti639 – 6391M → N in BAC97842. (PubMed:14621295)Curated
    Sequence conflicti639 – 6391M → N in AAH43924. (PubMed:15489334)Curated
    Sequence conflicti725 – 7251T → S in BAC39560. (PubMed:16141072)Curated
    Sequence conflicti725 – 7251T → S in AAH58087. (PubMed:15489334)Curated
    Sequence conflicti808 – 8081L → LRP in AAH43924. (PubMed:15489334)Curated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei606 – 62722LSSER…KVVPE → CSFAETEERCLGQLGAGVNG GE in isoform 3. 1 PublicationVSP_051825Add
    BLAST
    Alternative sequencei628 – 808181Missing in isoform 3. 1 PublicationVSP_051826Add
    BLAST
    Alternative sequencei758 – 7581V → G in isoform 2. 1 PublicationVSP_051827
    Alternative sequencei759 – 80850Missing in isoform 2. 1 PublicationVSP_051828Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB076696 mRNA. Translation: BAB97377.1.
    AK036384 mRNA. Translation: BAC29405.1.
    AK085882 mRNA. Translation: BAC39560.1.
    AK129032 mRNA. Translation: BAC97842.1. Different initiation.
    BC043924 mRNA. Translation: AAH43924.1.
    BC058087 mRNA. Translation: AAH58087.1.
    CCDSiCCDS26987.1. [Q8K4R9-1]
    RefSeqiNP_653136.2. NM_144553.2.
    UniGeneiMm.240543.

    Genome annotation databases

    EnsembliENSMUST00000043296; ENSMUSP00000040416; ENSMUSG00000037544.
    GeneIDi218977.
    KEGGimmu:218977.

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB076696 mRNA. Translation: BAB97377.1 .
    AK036384 mRNA. Translation: BAC29405.1 .
    AK085882 mRNA. Translation: BAC39560.1 .
    AK129032 mRNA. Translation: BAC97842.1 . Different initiation.
    BC043924 mRNA. Translation: AAH43924.1 .
    BC058087 mRNA. Translation: AAH58087.1 .
    CCDSi CCDS26987.1. [Q8K4R9-1 ]
    RefSeqi NP_653136.2. NM_144553.2.
    UniGenei Mm.240543.

    3D structure databases

    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 10090.ENSMUSP00000040416.

    PTM databases

    PhosphoSitei Q8K4R9.

    Proteomic databases

    PaxDbi Q8K4R9.
    PRIDEi Q8K4R9.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000043296 ; ENSMUSP00000040416 ; ENSMUSG00000037544 .
    GeneIDi 218977.
    KEGGi mmu:218977.

    Organism-specific databases

    CTDi 9787.
    MGIi MGI:2183453. Dlgap5.
    Rougei Search...

    Phylogenomic databases

    eggNOGi NOG270787.
    GeneTreei ENSGT00550000074473.
    HOGENOMi HOG000054220.
    HOVERGENi HBG062172.
    InParanoidi Q8K4R9.
    KOi K16804.
    OrthoDBi EOG75QR37.
    PhylomeDBi Q8K4R9.
    TreeFami TF321382.

    Miscellaneous databases

    NextBioi 376509.
    PROi Q8K4R9.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q8K4R9.
    Bgeei Q8K4R9.
    Genevestigatori Q8K4R9.

    Family and domain databases

    InterProi IPR005026. GKAP.
    [Graphical view ]
    Pfami PF03359. GKAP. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Identification of a novel cell cycle regulated gene, HURP, overexpressed in human hepatocellular carcinoma."
      Tsou A.-P., Yang C.-W., Huang C.-Y.F., Yu R.C.-T., Lee Y.-C.G., Chang C.-W., Chen B.-R., Chung Y.-F., Fann M.-J., Chi C.-W., Chiu J.-H., Chou C.-K.
      Oncogene 22:298-307(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), TISSUE SPECIFICITY.
    2. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 3).
      Strain: C57BL/6J.
      Tissue: Bone and Heart.
    3. "Prediction of the coding sequences of mouse homologues of KIAA gene: III. The complete nucleotide sequences of 500 mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries."
      Okazaki N., Kikuno R., Ohara R., Inamoto S., Koseki H., Hiraoka S., Saga Y., Nagase T., Ohara O., Koga H.
      DNA Res. 10:167-180(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
      Tissue: Embryonic tailImported.
    4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
      Strain: C57BL/6Imported.
      Tissue: BrainImported and Mammary glandImported.
    5. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-328, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Liver.

    Entry informationi

    Entry nameiDLGP5_MOUSE
    AccessioniPrimary (citable) accession number: Q8K4R9
    Secondary accession number(s): Q6ZQL3
    , Q80VS7, Q8BUC8, Q8BZ79
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: September 13, 2005
    Last sequence update: September 13, 2005
    Last modified: October 1, 2014
    This is version 87 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3