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Protein

Collectin-12

Gene

Colec12

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Scavenger receptor that displays several functions associated with host defense. Promotes binding and phagocytosis of Gram-positive, Gram-negative bacteria and yeast. Binds also to sialyl Lewis X or a trisaccharide and asialo-orosomucoid (ASOR). Mediates the recognition, internalization and degradation of oxidatively modified low density lipoprotein (oxLDL) by vascular endothelial cells (By similarity). Binds to several carbohydrates including Gal-type ligands, D-galactose, L- and D-fucose, GalNAc, T and Tn antigens in a calcium-dependent manner and internalizes specifically GalNAc in nurse-like cells (By similarity).By similarity1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi644Calcium 1; via carbonyl oxygen1
Metal bindingi646Calcium 11
Metal bindingi650Calcium 11
Metal bindingi670Calcium 21
Metal bindingi674Calcium 21
Binding sitei691Carbohydrate1
Metal bindingi694Calcium 31
Binding sitei694Carbohydrate1
Metal bindingi696Calcium 31
Binding sitei696Carbohydrate1
Metal bindingi697Calcium 21
Metal bindingi706Calcium 2; via carbonyl oxygen1
Metal bindingi706Calcium 31
Binding sitei706Carbohydrate1
Metal bindingi707Calcium 21
Metal bindingi718Calcium 31
Binding sitei718Carbohydrate1
Metal bindingi719Calcium 31
Binding sitei719Carbohydrate; via carbonyl oxygen1
Metal bindingi731Calcium 11

GO - Molecular functioni

  • carbohydrate binding Source: MGI
  • low-density lipoprotein particle binding Source: UniProtKB
  • metal ion binding Source: UniProtKB-KW
  • signaling pattern recognition receptor activity Source: UniProtKB

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Receptor

Keywords - Ligandi

Calcium, Lectin, Metal-binding

Enzyme and pathway databases

ReactomeiR-MMU-3000480. Scavenging by Class A Receptors.

Names & Taxonomyi

Protein namesi
Recommended name:
Collectin-12
Alternative name(s):
Collectin placenta protein 1
Short name:
CL-P1
Scavenger receptor with C-type lectin
Gene namesi
Name:Colec12
Synonyms:Clp1, Srcl
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 18

Organism-specific databases

MGIiMGI:2152907. Colec12.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 37CytoplasmicSequence analysisAdd BLAST37
Transmembranei38 – 58Helical; Signal-anchor for type II membrane proteinSequence analysisAdd BLAST21
Topological domaini59 – 742ExtracellularSequence analysisAdd BLAST684

GO - Cellular componenti

  • collagen trimer Source: UniProtKB-KW
  • extracellular exosome Source: MGI
  • integral component of membrane Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003186821 – 742Collectin-12Add BLAST742

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi67N-linked (GlcNAc...)1 Publication1
Glycosylationi159N-linked (GlcNAc...)1 Publication1
Glycosylationi160N-linked (GlcNAc...); atypical1 Publication1
Glycosylationi168N-linked (GlcNAc...)1 Publication1
Glycosylationi271N-linked (GlcNAc...)1 Publication1
Disulfide bondi607 ↔ 618PROSITE-ProRule annotation1 Publication
Disulfide bondi635 ↔ 730PROSITE-ProRule annotation1 Publication
Disulfide bondi708 ↔ 722PROSITE-ProRule annotation1 Publication

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

MaxQBiQ8K4Q8.
PaxDbiQ8K4Q8.
PeptideAtlasiQ8K4Q8.
PRIDEiQ8K4Q8.

PTM databases

iPTMnetiQ8K4Q8.
PhosphoSitePlusiQ8K4Q8.

Expressioni

Tissue specificityi

Expressed in vascular endothelial cells in the heart, in perivascular macrophage and smooth muscle cells. Expressed in plaques-surrounding reactive astrocytes located in cerebral cortex and hippocampus and in leptomeningeal vessels showing characteristics of cerebral amyloid angiopathy (CAA) in a double transgenic mouse model of Alzheimer disease (at protein level). Strongly expressed in lung. Moderately expressed in heart, skeletal muscle, spleen, liver, brain, colon, testis, stomach and kidney. Expressed in neonatal astrocytes. Expressed in reactive astrocytes and vascular/perivascular cells in the brain of a double transgenic mouse model of Alzheimer disease.3 Publications

Developmental stagei

Expressed in embryo at 9 dpc, increases progressively to a peak at 14 dpc and gradually decreases until 19 dpc.1 Publication

Inductioni

Up-regulated in activated microglia and by fibrillar beta amyloid peptide in activated astrocytes.1 Publication

Gene expression databases

BgeeiENSMUSG00000036103.
CleanExiMM_COLEC12.
GenevisibleiQ8K4Q8. MM.

Interactioni

Subunit structurei

The extracellular domain forms a stable trimer (By similarity). The extracellular domain interacts with fibrillar beta amyloid peptide.By similarity2 Publications

Protein-protein interaction databases

IntActiQ8K4Q8. 1 interactor.
MINTiMINT-4120297.
STRINGi10090.ENSMUSP00000043220.

Structurei

Secondary structure

1742
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi612 – 614Combined sources3
Beta strandi617 – 621Combined sources5
Helixi628 – 637Combined sources10
Helixi648 – 656Combined sources9
Beta strandi660 – 662Combined sources3
Beta strandi664 – 669Combined sources6
Beta strandi671 – 673Combined sources3
Turni698 – 702Combined sources5
Beta strandi708 – 711Combined sources4
Helixi713 – 715Combined sources3
Beta strandi717 – 720Combined sources4
Beta strandi726 – 733Combined sources8

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2OX9X-ray1.95A/B/C/D603-742[»]
ProteinModelPortaliQ8K4Q8.
SMRiQ8K4Q8.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ8K4Q8.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini452 – 511Collagen-like 1Add BLAST60
Domaini527 – 586Collagen-like 2Add BLAST60
Domaini614 – 731C-type lectinPROSITE-ProRule annotationAdd BLAST118

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili73 – 142Sequence analysisAdd BLAST70
Coiled coili205 – 254Sequence analysisAdd BLAST50

Sequence similaritiesi

Contains 1 C-type lectin domain.PROSITE-ProRule annotation
Contains 2 collagen-like domains.Curated

Keywords - Domaini

Coiled coil, Collagen, Repeat, Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IIUI. Eukaryota.
ENOG41101BT. LUCA.
GeneTreeiENSGT00820000126981.
HOGENOMiHOG000111886.
HOVERGENiHBG107745.
InParanoidiQ8K4Q8.
KOiK10062.
OMAiEQQWIKK.
OrthoDBiEOG091G0OLC.
PhylomeDBiQ8K4Q8.
TreeFamiTF332426.

Family and domain databases

Gene3Di3.10.100.10. 2 hits.
InterProiIPR001304. C-type_lectin-like.
IPR016186. C-type_lectin-like/link.
IPR018378. C-type_lectin_CS.
IPR008160. Collagen.
IPR016187. CTDL_fold.
[Graphical view]
PfamiPF01391. Collagen. 3 hits.
PF00059. Lectin_C. 1 hit.
[Graphical view]
SMARTiSM00034. CLECT. 1 hit.
[Graphical view]
SUPFAMiSSF56436. SSF56436. 1 hit.
PROSITEiPS00615. C_TYPE_LECTIN_1. 1 hit.
PS50041. C_TYPE_LECTIN_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8K4Q8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKDDFAEEEE VQSFGYKRFG IQEGTQCTKC KNNWALKFSI VLLYILCALL
60 70 80 90 100
TITVAILGYK VVEKMDNVTD GMETSHQTYD NKLTAVESDL KKLGDQAGKK
110 120 130 140 150
ALSTNSELST FRSDILDLRQ QLQEITEKTS KNKDTLEKLQ ANGDSLVDRQ
160 170 180 190 200
SQLKETLQNN SFLITTVNKT LQAYNGYVTN LQQDTSVLQG NLQSQMYSQS
210 220 230 240 250
VVIMNLNNLN LTQVQQRNLI SNLQQSVDDT SLAIQRIKND FQNLQQVFLQ
260 270 280 290 300
AKKDTDWLKE KVQSLQTLAA NNSALAKANN DTLEDMNSQL SSFTGQMDNI
310 320 330 340 350
TTISQANEQS LKDLQDLHKD TENRTAVKFS QLEERFQVFE TDIVNIISNI
360 370 380 390 400
SYTAHHLRTL TSNLNDVRTT CTDTLTRHTD DLTSLNNTLV NIRLDSISLR
410 420 430 440 450
MQQDMMRSKL DTEVANLSVV MEEMKLVDSK HGQLIKNFTI LQGPPGPRGP
460 470 480 490 500
KGDRGSQGPP GPTGNKGQKG EKGEPGPPGP AGERGTIGPV GPPGERGSKG
510 520 530 540 550
SKGSQGPKGS RGSPGKPGPQ GPSGDPGPPG PPGKDGLPGP QGPPGFQGLQ
560 570 580 590 600
GTVGEPGVPG PRGLPGLPGV PGMPGPKGPP GPPGPSGAME PLALQNEPTP
610 620 630 640 650
ASEVNGCPPH WKNFTDKCYY FSLEKEIFED AKLFCEDKSS HLVFINSREE
660 670 680 690 700
QQWIKKHTVG RESHWIGLTD SEQESEWKWL DGSPVDYKNW KAGQPDNWGS
710 720 730 740
GHGPGEDCAG LIYAGQWNDF QCDEINNFIC EKEREAVPSS IL
Length:742
Mass (Da):81,304
Last modified:October 1, 2002 - v1
Checksum:i1537C490E5911C45
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti22Q → H in BAB82497 (PubMed:11718900).Curated1
Sequence conflicti31K → I in BAB82497 (PubMed:11718900).Curated1
Sequence conflicti69T → S in BAB82497 (PubMed:11718900).Curated1
Sequence conflicti182Q → L in BAC31361 (PubMed:16141072).Curated1
Sequence conflicti186S → N in BAB82497 (PubMed:11718900).Curated1
Sequence conflicti368R → W in BAB82497 (PubMed:11718900).Curated1
Sequence conflicti628F → L in BAB82497 (PubMed:11718900).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB038519 mRNA. Translation: BAB82497.1.
AB078434 mRNA. Translation: BAC05523.1.
AK042772 mRNA. Translation: BAC31361.1.
AK158366 mRNA. Translation: BAE34474.1.
BC057936 mRNA. Translation: AAH57936.1.
CCDSiCCDS37732.1.
RefSeqiNP_569716.2. NM_130449.2.
UniGeneiMm.218571.

Genome annotation databases

EnsembliENSMUST00000040069; ENSMUSP00000043220; ENSMUSG00000036103.
GeneIDi140792.
KEGGimmu:140792.
UCSCiuc008eak.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB038519 mRNA. Translation: BAB82497.1.
AB078434 mRNA. Translation: BAC05523.1.
AK042772 mRNA. Translation: BAC31361.1.
AK158366 mRNA. Translation: BAE34474.1.
BC057936 mRNA. Translation: AAH57936.1.
CCDSiCCDS37732.1.
RefSeqiNP_569716.2. NM_130449.2.
UniGeneiMm.218571.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2OX9X-ray1.95A/B/C/D603-742[»]
ProteinModelPortaliQ8K4Q8.
SMRiQ8K4Q8.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ8K4Q8. 1 interactor.
MINTiMINT-4120297.
STRINGi10090.ENSMUSP00000043220.

PTM databases

iPTMnetiQ8K4Q8.
PhosphoSitePlusiQ8K4Q8.

Proteomic databases

MaxQBiQ8K4Q8.
PaxDbiQ8K4Q8.
PeptideAtlasiQ8K4Q8.
PRIDEiQ8K4Q8.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000040069; ENSMUSP00000043220; ENSMUSG00000036103.
GeneIDi140792.
KEGGimmu:140792.
UCSCiuc008eak.2. mouse.

Organism-specific databases

CTDi81035.
MGIiMGI:2152907. Colec12.

Phylogenomic databases

eggNOGiENOG410IIUI. Eukaryota.
ENOG41101BT. LUCA.
GeneTreeiENSGT00820000126981.
HOGENOMiHOG000111886.
HOVERGENiHBG107745.
InParanoidiQ8K4Q8.
KOiK10062.
OMAiEQQWIKK.
OrthoDBiEOG091G0OLC.
PhylomeDBiQ8K4Q8.
TreeFamiTF332426.

Enzyme and pathway databases

ReactomeiR-MMU-3000480. Scavenging by Class A Receptors.

Miscellaneous databases

ChiTaRSiColec12. mouse.
EvolutionaryTraceiQ8K4Q8.
PROiQ8K4Q8.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000036103.
CleanExiMM_COLEC12.
GenevisibleiQ8K4Q8. MM.

Family and domain databases

Gene3Di3.10.100.10. 2 hits.
InterProiIPR001304. C-type_lectin-like.
IPR016186. C-type_lectin-like/link.
IPR018378. C-type_lectin_CS.
IPR008160. Collagen.
IPR016187. CTDL_fold.
[Graphical view]
PfamiPF01391. Collagen. 3 hits.
PF00059. Lectin_C. 1 hit.
[Graphical view]
SMARTiSM00034. CLECT. 1 hit.
[Graphical view]
SUPFAMiSSF56436. SSF56436. 1 hit.
PROSITEiPS00615. C_TYPE_LECTIN_1. 1 hit.
PS50041. C_TYPE_LECTIN_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCOL12_MOUSE
AccessioniPrimary (citable) accession number: Q8K4Q8
Secondary accession number(s): Q3TYT8, Q8C979, Q8VIF6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 26, 2008
Last sequence update: October 1, 2002
Last modified: November 30, 2016
This is version 124 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.