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Protein

Ceramide kinase

Gene

Cerk

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Catalyzes specifically the phosphorylation of ceramide to form ceramide 1-phosphate. Acts efficiently on natural and analog ceramides (C6, C8, C16 ceramides, and C8-dihydroceramide), to a lesser extent on C2-ceramide and C6-dihydroceramide, but not on other lipids, such as various sphingosines (By similarity).By similarity

Catalytic activityi

ATP + ceramide = ADP + ceramide 1-phosphate.

Cofactori

Protein has several cofactor binding sites:

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei197 – 1971Proton donor/acceptorBy similarity
Binding sitei202 – 2021ATPPROSITE-ProRule annotation
Binding sitei304 – 3041ATPPROSITE-ProRule annotation
Binding sitei310 – 3101ATPPROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi138 – 1403ATPPROSITE-ProRule annotation
Nucleotide bindingi170 – 1745ATPPROSITE-ProRule annotation
Nucleotide bindingi239 – 2413ATPPROSITE-ProRule annotation
Nucleotide bindingi502 – 5043ATPPROSITE-ProRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Ligandi

ATP-binding, Calcium, Magnesium, Nucleotide-binding

Enzyme and pathway databases

BRENDAi2.7.1.138. 3474.
ReactomeiR-MMU-1660662. Glycosphingolipid metabolism.

Names & Taxonomyi

Protein namesi
Recommended name:
Ceramide kinase (EC:2.7.1.138)
Short name:
mCERK
Alternative name(s):
Acylsphingosine kinase
Gene namesi
Name:Cerk
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 15

Organism-specific databases

MGIiMGI:2386052. Cerk.

Subcellular locationi

GO - Cellular componenti

  • integral component of membrane Source: UniProtKB
  • membrane Source: MGI
  • mitochondrion Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 531531Ceramide kinasePRO_0000181355Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei340 – 3401PhosphoserineBy similarity
Modified residuei408 – 4081PhosphoserineBy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ8K4Q7.
PaxDbiQ8K4Q7.
PRIDEiQ8K4Q7.

PTM databases

PhosphoSiteiQ8K4Q7.

Expressioni

Tissue specificityi

High level expression in heart, brain and testis; low expression in spleen, liver and lung; not detected in skeletal muscle.

Developmental stagei

Highly expressed at 7 dpc and decreases rapidly thereafter.

Gene expression databases

BgeeiQ8K4Q7.
CleanExiMM_CERK.
ExpressionAtlasiQ8K4Q7. baseline and differential.
GenevisibleiQ8K4Q7. MM.

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000038203.

Structurei

3D structure databases

ProteinModelPortaliQ8K4Q7.
SMRiQ8K4Q7. Positions 120-333.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini128 – 278151DAGKcPROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 125125Required for binding to sulfatide and phosphoinositidesBy similarityAdd
BLAST
Regioni195 – 1984Substrate bindingBy similarity

Sequence similaritiesi

Contains 1 DAGKc domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG1115. Eukaryota.
COG1597. LUCA.
GeneTreeiENSGT00690000101761.
HOGENOMiHOG000111551.
HOVERGENiHBG050903.
InParanoidiQ8K4Q7.
KOiK04715.
OMAiINGLIFR.
OrthoDBiEOG738048.
TreeFamiTF314514.

Family and domain databases

InterProiIPR001206. Diacylglycerol_kinase_cat_dom.
IPR016064. NAD/diacylglycerol_kinase.
[Graphical view]
PfamiPF00781. DAGK_cat. 1 hit.
[Graphical view]
SMARTiSM00046. DAGKc. 1 hit.
[Graphical view]
SUPFAMiSSF111331. SSF111331. 3 hits.
PROSITEiPS50146. DAGK. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8K4Q7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGAMGAAEPL HSVLWVKRRR CAVSLEPARA LLRWWRSPEP GPSAPGADAR
60 70 80 90 100
SVLVSEIIAV EEKDDCEKHA SSGRWHKMEN PFAFTVHRVK RVRHHRWKWA
110 120 130 140 150
RVTFWSADEQ LCHLWLQTLR GLLESLTSRP KHLLVFINPF GGKGQGKRIY
160 170 180 190 200
EKTVAPLFTL ASITTEIIIT EHANQAKETL YEINTDSYDG IVCVGGDGMF
210 220 230 240 250
SEVLHGVIGR TQQSAGIDPN HPRAVLVPST LRIGIIPAGS TDCVCYSTVG
260 270 280 290 300
TNDAETSALH IIIGDSLAID VSSVHYHNTL LRYSVSLLGY GFYGDLIKDS
310 320 330 340 350
EKKRWMGLVR YDFSGLKTFL SHQYYEGTLS FLPAQHTVGS PRDNKPCRAG
360 370 380 390 400
CFVCRQSKQQ LEEEEKKALY GLENAEEMEE WQVTCGKFLA INATNMSCAC
410 420 430 440 450
PRSPGGLSPF AHLGDGSSDL ILIRKCSRFN FLRFLIRHTN QEDQFDFTFV
460 470 480 490 500
EVYRVKKFHF TSKHVEDEDN DSKEQEKQKF GKICKDRPSC TCSASRSSWN
510 520 530
CDGEVMHSPA IEVRVHCQLV RLFARGIEEE S
Length:531
Mass (Da):59,843
Last modified:July 27, 2011 - v2
Checksum:iC5A030D422E3AA10
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti378 – 3781M → V in BAC01155 (PubMed:11956206).Curated
Sequence conflicti467 – 4671D → Y in BAC34908 (PubMed:16141072).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB079067 mRNA. Translation: BAC01155.1.
AK042077 mRNA. Translation: BAC31157.1.
AK052269 mRNA. Translation: BAC34908.1.
AK169528 mRNA. Translation: BAE41212.1.
BC094253 mRNA. Translation: AAH94253.1.
CCDSiCCDS27728.1.
RefSeqiNP_663450.3. NM_145475.4.
UniGeneiMm.222685.

Genome annotation databases

EnsembliENSMUST00000044332; ENSMUSP00000038203; ENSMUSG00000035891.
GeneIDi223753.
KEGGimmu:223753.
UCSCiuc007xdx.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB079067 mRNA. Translation: BAC01155.1.
AK042077 mRNA. Translation: BAC31157.1.
AK052269 mRNA. Translation: BAC34908.1.
AK169528 mRNA. Translation: BAE41212.1.
BC094253 mRNA. Translation: AAH94253.1.
CCDSiCCDS27728.1.
RefSeqiNP_663450.3. NM_145475.4.
UniGeneiMm.222685.

3D structure databases

ProteinModelPortaliQ8K4Q7.
SMRiQ8K4Q7. Positions 120-333.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000038203.

PTM databases

PhosphoSiteiQ8K4Q7.

Proteomic databases

MaxQBiQ8K4Q7.
PaxDbiQ8K4Q7.
PRIDEiQ8K4Q7.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000044332; ENSMUSP00000038203; ENSMUSG00000035891.
GeneIDi223753.
KEGGimmu:223753.
UCSCiuc007xdx.2. mouse.

Organism-specific databases

CTDi64781.
MGIiMGI:2386052. Cerk.

Phylogenomic databases

eggNOGiKOG1115. Eukaryota.
COG1597. LUCA.
GeneTreeiENSGT00690000101761.
HOGENOMiHOG000111551.
HOVERGENiHBG050903.
InParanoidiQ8K4Q7.
KOiK04715.
OMAiINGLIFR.
OrthoDBiEOG738048.
TreeFamiTF314514.

Enzyme and pathway databases

BRENDAi2.7.1.138. 3474.
ReactomeiR-MMU-1660662. Glycosphingolipid metabolism.

Miscellaneous databases

ChiTaRSiCerk. mouse.
NextBioi376874.
PROiQ8K4Q7.
SOURCEiSearch...

Gene expression databases

BgeeiQ8K4Q7.
CleanExiMM_CERK.
ExpressionAtlasiQ8K4Q7. baseline and differential.
GenevisibleiQ8K4Q7. MM.

Family and domain databases

InterProiIPR001206. Diacylglycerol_kinase_cat_dom.
IPR016064. NAD/diacylglycerol_kinase.
[Graphical view]
PfamiPF00781. DAGK_cat. 1 hit.
[Graphical view]
SMARTiSM00046. DAGKc. 1 hit.
[Graphical view]
SUPFAMiSSF111331. SSF111331. 3 hits.
PROSITEiPS50146. DAGK. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Ceramide kinase, a novel lipid kinase. Molecular cloning and functional characterization."
    Sugiura M., Kono K., Liu H., Shimizugawa T., Minekura H., Spiegel S., Kohama T.
    J. Biol. Chem. 277:23294-23300(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Brain.
  2. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Heart and Thymus.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Brain.

Entry informationi

Entry nameiCERK1_MOUSE
AccessioniPrimary (citable) accession number: Q8K4Q7
Secondary accession number(s): Q52KP2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 23, 2003
Last sequence update: July 27, 2011
Last modified: May 11, 2016
This is version 109 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.