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Q8K4Q6

- NEIL1_MOUSE

UniProt

Q8K4Q6 - NEIL1_MOUSE

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Protein

Endonuclease 8-like 1

Gene
Neil1, Nei1
Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at transcript leveli

Functioni

Involved in base excision repair of DNA damaged by oxidation or by mutagenic agents. Acts as DNA glycosylase that recognizes and removes damaged bases. Has a preference for oxidized pyrimidines, such as thymine glycol, formamidopyrimidine (Fapy) and 5-hydroxyuracil. Has marginal activity towards 8-oxoguanine. Has AP (apurinic/apyrimidinic) lyase activity and introduces nicks in the DNA strand. Cleaves the DNA backbone by beta-delta elimination to generate a single-strand break at the site of the removed base with both 3'- and 5'-phosphates. Has DNA glycosylase/lyase activity towards mismatched uracil and thymine, in particular in U:C and T:C mismatches. Specifically binds 5-hydroxymethylcytosine (5hmC), suggesting that it acts as a specific reader of 5hmC.2 Publications

Catalytic activityi

Removes damaged bases from DNA, leaving an abasic site.
The C-O-P bond 3' to the apurinic or apyrimidinic site in DNA is broken by a beta-elimination reaction, leaving a 3'-terminal unsaturated sugar and a product with a terminal 5'-phosphate.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei2 – 21Schiff-base intermediate with DNA Inferred
Active sitei3 – 31Proton donor Inferred
Active sitei54 – 541Proton donor; for beta-elimination activity Inferred
Binding sitei176 – 1761DNA By similarity

GO - Molecular functioni

  1. damaged DNA binding Source: InterPro
  2. DNA-(apurinic or apyrimidinic site) lyase activity Source: UniProtKB-EC
  3. DNA N-glycosylase activity Source: MGI
  4. lyase activity Source: MGI
  5. zinc ion binding Source: InterPro

GO - Biological processi

  1. base-excision repair Source: InterPro
  2. DNA repair Source: MGI
  3. negative regulation of nuclease activity Source: Ensembl
  4. nucleotide-excision repair Source: InterPro
  5. response to oxidative stress Source: Ensembl
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase, Lyase

Keywords - Biological processi

DNA damage, DNA repair

Keywords - Ligandi

DNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Endonuclease 8-like 1 (EC:3.2.2.-, EC:4.2.99.18)
Alternative name(s):
DNA glycosylase/AP lyase Neil1
DNA-(apurinic or apyrimidinic site) lyase Neil1
Endonuclease VIII-like 1
Nei homolog 1
Short name:
NEH1
Nei-like protein 1
Gene namesi
Name:Neil1
Synonyms:Nei1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 9

Organism-specific databases

MGIiMGI:1920024. Neil1.

Subcellular locationi

Cytoplasmcytoskeletonmicrotubule organizing centercentrosome. Nucleus. Chromosome
Note: During mitosis, associates with centrosomes and condensed chromatin By similarity.

GO - Cellular componenti

  1. chromosome Source: UniProtKB-SubCell
  2. cytoplasm Source: UniProtKB-KW
  3. microtubule organizing center Source: UniProtKB-SubCell
  4. nucleus Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Chromosome, Cytoplasm, Cytoskeleton, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed By similarity
Chaini2 – 389388Endonuclease 8-like 1PRO_0000170906Add
BLAST

Proteomic databases

PRIDEiQ8K4Q6.

PTM databases

PhosphoSiteiQ8K4Q6.

Expressioni

Tissue specificityi

Detected in heart, spleen and lung.1 Publication

Inductioni

Up-regulated during S-phase By similarity.

Gene expression databases

BgeeiQ8K4Q6.
CleanExiMM_NEIL1.
GenevestigatoriQ8K4Q6.

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000034842.

Structurei

3D structure databases

ProteinModelPortaliQ8K4Q6.
SMRiQ8K4Q6. Positions 2-290.

Family & Domainsi

Sequence similaritiesi

Belongs to the FPG family.

Phylogenomic databases

eggNOGiNOG75119.
GeneTreeiENSGT00390000016671.
HOGENOMiHOG000067872.
HOVERGENiHBG052592.
InParanoidiQ8K4Q6.
KOiK10567.
OMAiHLASHFV.
OrthoDBiEOG7H1JM2.
PhylomeDBiQ8K4Q6.
TreeFamiTF333272.

Family and domain databases

InterProiIPR015886. DNA_glyclase/AP_lyase_DNA-bd.
IPR012319. DNA_glycosylase/AP_lyase_cat.
IPR015371. Endonuclease-VIII_DNA-bd.
IPR010979. Ribosomal_S13-like_H2TH.
[Graphical view]
PfamiPF01149. Fapy_DNA_glyco. 1 hit.
PF06831. H2TH. 1 hit.
PF09292. Neil1-DNA_bind. 1 hit.
[Graphical view]
SMARTiSM00898. Fapy_DNA_glyco. 1 hit.
[Graphical view]
SUPFAMiSSF46946. SSF46946. 1 hit.
SSF81624. SSF81624. 1 hit.
PROSITEiPS51068. FPG_CAT. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: Q8K4Q6-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

MPEGPELHLA SHFVNETCKG LVFGGCVEKS SVSRNPEVPF ESSAYHISAL    50
ARGKELRLTL SPLPGSQPPQ KPLSLVFRFG MSGSFQLVPA EALPRHAHLR 100
FYTAPPAPRL ALCFVDIRRF GHWDPGGEWQ PGRGPCVLLE YERFRENVLR 150
NLSDKAFDRP ICEALLDQRF FNGIGNYLRA EILYRLKIPP FEKARTVLEA 200
LQQCRPSPEL TLSQKIKAKL QNPDLLELCH LVPKEVVQLG GKGYGPERGE 250
EDFAAFRAWL RCYGVPGMSS LRDRHGRTIW FQGDPGPLAP KGGRSQKKKS 300
QETQLGAEDR KEDLPLSSKS VSRMRRARKH PPKRIAQQSE GAGLQQNQET 350
PTAPEKGKRR GQRASTGHRR RPKTIPDTRP REAGESSAS 389
Length:389
Mass (Da):43,586
Last modified:January 23, 2007 - v3
Checksum:iE3F1A21DDE2CB5AD
GO
Isoform 2 (identifier: Q8K4Q6-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     241-389: GKGYGPERGE...PREAGESSAS → EAWGGQDGRRPLP

Note: No experimental confirmation available.

Show »
Length:253
Mass (Da):28,453
Checksum:i8BE7DE61651A166A
GO

Sequence cautioni

The sequence BAB28790.1 differs from that shown. Reason: Frameshift at position 344.

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei241 – 389149GKGYG…ESSAS → EAWGGQDGRRPLP in isoform 2. VSP_012207Add
BLAST

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB079069 mRNA. Translation: BAC06477.1.
AK013322 mRNA. Translation: BAB28790.1. Frameshift.
BC043297 mRNA. Translation: AAH43297.1.
CCDSiCCDS23217.1. [Q8K4Q6-1]
RefSeqiNP_082623.1. NM_028347.2. [Q8K4Q6-1]
XP_006511547.1. XM_006511484.1. [Q8K4Q6-1]
XP_006511548.1. XM_006511485.1. [Q8K4Q6-1]
XP_006511549.1. XM_006511486.1. [Q8K4Q6-1]
UniGeneiMm.35749.
Mm.488747.

Genome annotation databases

EnsembliENSMUST00000034842; ENSMUSP00000034842; ENSMUSG00000032298. [Q8K4Q6-1]
GeneIDi72774.
KEGGimmu:72774.
UCSCiuc009puh.1. mouse. [Q8K4Q6-1]
uc009puk.1. mouse. [Q8K4Q6-2]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB079069 mRNA. Translation: BAC06477.1 .
AK013322 mRNA. Translation: BAB28790.1 . Frameshift.
BC043297 mRNA. Translation: AAH43297.1 .
CCDSi CCDS23217.1. [Q8K4Q6-1 ]
RefSeqi NP_082623.1. NM_028347.2. [Q8K4Q6-1 ]
XP_006511547.1. XM_006511484.1. [Q8K4Q6-1 ]
XP_006511548.1. XM_006511485.1. [Q8K4Q6-1 ]
XP_006511549.1. XM_006511486.1. [Q8K4Q6-1 ]
UniGenei Mm.35749.
Mm.488747.

3D structure databases

ProteinModelPortali Q8K4Q6.
SMRi Q8K4Q6. Positions 2-290.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 10090.ENSMUSP00000034842.

PTM databases

PhosphoSitei Q8K4Q6.

Proteomic databases

PRIDEi Q8K4Q6.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000034842 ; ENSMUSP00000034842 ; ENSMUSG00000032298 . [Q8K4Q6-1 ]
GeneIDi 72774.
KEGGi mmu:72774.
UCSCi uc009puh.1. mouse. [Q8K4Q6-1 ]
uc009puk.1. mouse. [Q8K4Q6-2 ]

Organism-specific databases

CTDi 79661.
MGIi MGI:1920024. Neil1.

Phylogenomic databases

eggNOGi NOG75119.
GeneTreei ENSGT00390000016671.
HOGENOMi HOG000067872.
HOVERGENi HBG052592.
InParanoidi Q8K4Q6.
KOi K10567.
OMAi HLASHFV.
OrthoDBi EOG7H1JM2.
PhylomeDBi Q8K4Q6.
TreeFami TF333272.

Miscellaneous databases

NextBioi 336897.
PROi Q8K4Q6.
SOURCEi Search...

Gene expression databases

Bgeei Q8K4Q6.
CleanExi MM_NEIL1.
Genevestigatori Q8K4Q6.

Family and domain databases

InterProi IPR015886. DNA_glyclase/AP_lyase_DNA-bd.
IPR012319. DNA_glycosylase/AP_lyase_cat.
IPR015371. Endonuclease-VIII_DNA-bd.
IPR010979. Ribosomal_S13-like_H2TH.
[Graphical view ]
Pfami PF01149. Fapy_DNA_glyco. 1 hit.
PF06831. H2TH. 1 hit.
PF09292. Neil1-DNA_bind. 1 hit.
[Graphical view ]
SMARTi SM00898. Fapy_DNA_glyco. 1 hit.
[Graphical view ]
SUPFAMi SSF46946. SSF46946. 1 hit.
SSF81624. SSF81624. 1 hit.
PROSITEi PS51068. FPG_CAT. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "A back-up glycosylase in Nth1 knock-out mice is a functional Nei (endonuclease VIII) homologue."
    Takao M., Kanno S., Kobayashi K., Zhang Q.-M., Yonei S., van der Horst G.T.J., Yasui A.
    J. Biol. Chem. 277:42205-42213(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, TISSUE SPECIFICITY.
    Tissue: Liver.
  2. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Strain: C57BL/6J.
    Tissue: Embryo.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    Strain: FVB/N.
    Tissue: Mammary gland.
  4. Cited for: FUNCTION.

Entry informationi

Entry nameiNEIL1_MOUSE
AccessioniPrimary (citable) accession number: Q8K4Q6
Secondary accession number(s): Q80V58, Q9CYT9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 7, 2004
Last sequence update: January 23, 2007
Last modified: July 9, 2014
This is version 100 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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