Q8K4Q6 (NEIL1_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Endonuclease 8-like 1
EC=3.2.2.-
EC=4.2.99.18

Alternative name(s):
DNA glycosylase/AP lyase Neil1
DNA-(apurinic or apyrimidinic site) lyase Neil1
Endonuclease VIII-like 1
Nei homolog 1
Short name=NEH1
Nei-like protein 1

Gene names

Name:	Neil1
Synonyms:	Nei1

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	389 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Involved in base excision repair of DNA damaged by oxidation or by mutagenic agents. Acts as DNA glycosylase that recognizes and removes damaged bases. Has a preference for oxidized pyrimidines, such as thymine glycol, formamidopyrimidine (Fapy) and 5-hydroxyuracil. Has marginal activity towards 8-oxoguanine. Has AP (apurinic/apyrimidinic) lyase activity and introduces nicks in the DNA strand. Cleaves the DNA backbone by beta-delta elimination to generate a single-strand break at the site of the removed base with both 3'- and 5'-phosphates. Has DNA glycosylase/lyase activity towards mismatched uracil and thymine, in particular in U:C and T:C mismatches By similarity. Ref.1
Catalytic activity	Removes damaged bases from DNA, leaving an abasic site. The C-O-P bond 3' to the apurinic or apyrimidinic site in DNA is broken by a beta-elimination reaction, leaving a 3'-terminal unsaturated sugar and a product with a terminal 5'-phosphate.
Subcellular location	Cytoplasm › cytoskeleton › centrosome. Nucleus. Chromosome. Note: During mitosis, associates with centrosomes and condensed chromatin By similarity.
Tissue specificity	Detected in heart, spleen and lung. Ref.1
Induction	Up-regulated during S-phase By similarity.
Sequence similarities	Belongs to the FPG family.
Sequence caution	The sequence BAB28790.1 differs from that shown. Reason: Frameshift at position 344.

Ontologies

Keywords
Biological process	DNA damage DNA repair
Cellular component	Chromosome Cytoplasm Cytoskeleton Nucleus
Coding sequence diversity	Alternative splicing
Ligand	DNA-binding
Molecular function	Glycosidase Hydrolase Lyase
Technical term	Complete proteome Multifunctional enzyme Reference proteome
Gene Ontology (GO)
Biological_process	DNA repair Inferred from direct assay Ref.1. Source: MGI base-excision repair Inferred from electronic annotation. Source: InterPro negative regulation of nuclease activity Inferred from electronic annotation. Source: Compara nucleotide-excision repair Inferred from electronic annotation. Source: InterPro response to oxidative stress Inferred from electronic annotation. Source: Compara
Cellular_component	chromosome Inferred from electronic annotation. Source: UniProtKB-SubCell cytoplasm Inferred from electronic annotation. Source: UniProtKB-KW microtubule organizing center Inferred from electronic annotation. Source: UniProtKB-SubCell nucleus Inferred from direct assay Ref.1. Source: MGI
Molecular_function	DNA N-glycosylase activity Inferred from direct assay Ref.1. Source: MGI DNA-(apurinic or apyrimidinic site) lyase activity Inferred from electronic annotation. Source: EC damaged DNA binding Inferred from electronic annotation. Source: InterPro lyase activity Inferred from direct assay Ref.1. Source: MGI zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q8K4Q6-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q8K4Q6-2)

The sequence of this isoform differs from the canonical sequence as follows:
     241-389: GKGYGPERGE...PREAGESSAS → EAWGGQDGRRPLP

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed By similarity
	Chain	2 – 389	388	Endonuclease 8-like 1		PRO_0000170906
Sites
	Active site	2	1	Schiff-base intermediate with DNA Probable
	Active site	3	1	Proton donor Probable
	Active site	54	1	Proton donor; for beta-elimination activity Probable
	Binding site	176	1	DNA By similarity
Natural variations
	Alternative sequence	241 – 389	149	GKGYG…ESSAS → EAWGGQDGRRPLP in isoform 2.		VSP_012207

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified January 23, 2007. Version 3. Checksum: E3F1A21DDE2CB5AD	FASTA	389	43,586
	10 20 30 40 50 60 MPEGPELHLA SHFVNETCKG LVFGGCVEKS SVSRNPEVPF ESSAYHISAL ARGKELRLTL 70 80 90 100 110 120 SPLPGSQPPQ KPLSLVFRFG MSGSFQLVPA EALPRHAHLR FYTAPPAPRL ALCFVDIRRF 130 140 150 160 170 180 GHWDPGGEWQ PGRGPCVLLE YERFRENVLR NLSDKAFDRP ICEALLDQRF FNGIGNYLRA 190 200 210 220 230 240 EILYRLKIPP FEKARTVLEA LQQCRPSPEL TLSQKIKAKL QNPDLLELCH LVPKEVVQLG 250 260 270 280 290 300 GKGYGPERGE EDFAAFRAWL RCYGVPGMSS LRDRHGRTIW FQGDPGPLAP KGGRSQKKKS 310 320 330 340 350 360 QETQLGAEDR KEDLPLSSKS VSRMRRARKH PPKRIAQQSE GAGLQQNQET PTAPEKGKRR 370 380 GQRASTGHRR RPKTIPDTRP REAGESSAS
	Isoform 2 [UniParc]. Checksum: 8BE7DE61651A166A	FASTA	253	28,453
	10 20 30 40 50 60 MPEGPELHLA SHFVNETCKG LVFGGCVEKS SVSRNPEVPF ESSAYHISAL ARGKELRLTL 70 80 90 100 110 120 SPLPGSQPPQ KPLSLVFRFG MSGSFQLVPA EALPRHAHLR FYTAPPAPRL ALCFVDIRRF 130 140 150 160 170 180 GHWDPGGEWQ PGRGPCVLLE YERFRENVLR NLSDKAFDRP ICEALLDQRF FNGIGNYLRA 190 200 210 220 230 240 EILYRLKIPP FEKARTVLEA LQQCRPSPEL TLSQKIKAKL QNPDLLELCH LVPKEVVQLG 250 EAWGGQDGRR PLP

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A back-up glycosylase in Nth1 knock-out mice is a functional Nei (endonuclease VIII) homologue." Takao M., Kanno S., Kobayashi K., Zhang Q.-M., Yonei S., van der Horst G.T.J., Yasui A. J. Biol. Chem. 277:42205-42213(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, TISSUE SPECIFICITY. Tissue: Liver.
[2]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Strain: C57BL/6J. Tissue: Embryo.
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Strain: FVB/N. Tissue: Mammary gland.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB079069 mRNA. Translation: BAC06477.1. AK013322 mRNA. Translation: BAB28790.1. Frameshift. BC043297 mRNA. Translation: AAH43297.1.
IPI	IPI00170254. IPI00480434.
RefSeq	NP_082623.1. NM_028347.2.
UniGene	Mm.35749. Mm.488747.
3D structure databases
ProteinModelPortal	Q8K4Q6.
SMR	Q8K4Q6. Positions 2-290.
ModBase	Search...
Protein-protein interaction databases
STRING	10090.ENSMUSP00000034842.
PTM databases
PhosphoSite	Q8K4Q6.
Proteomic databases
PRIDE	Q8K4Q6.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000034842; ENSMUSP00000034842; ENSMUSG00000032298.
GeneID	72774.
KEGG	mmu:72774.
UCSC	uc009puh.1. mouse. uc009puk.1. mouse.
Organism-specific databases
CTD	79661.
MGI	MGI:1920024. Neil1.
Phylogenomic databases
eggNOG	NOG75119.
GeneTree	ENSGT00390000016671.
HOGENOM	HOG000067872.
HOVERGEN	HBG052592.
InParanoid	Q8K4Q6.
KO	K10567.
OMA	GTSLQQD.
Gene expression databases
Bgee	Q8K4Q6.
CleanEx	MM_NEIL1.
Genevestigator	Q8K4Q6.
GermOnline	ENSMUSG00000032298. Mus musculus.
Family and domain databases
InterPro	IPR015886. DNA_glyclase/AP_lyase_DNA-bd. IPR012319. DNA_glycosylase/AP_lyase_cat. IPR015371. Endonuclease-VIII_DNA-bd. IPR010979. Ribosomal_S13-like_H2TH. [Graphical view]
Pfam	PF01149. Fapy_DNA_glyco. 1 hit. PF06831. H2TH. 1 hit. PF09292. Neil1-DNA_bind. 1 hit. [Graphical view]
SMART	SM00898. Fapy_DNA_glyco. 1 hit. [Graphical view]
SUPFAM	SSF81624. Form_DNAglyc_cat. 1 hit. SSF46946. Ribosomal_H2TH. 1 hit.
PROSITE	PS51068. FPG_CAT. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	336897.
SOURCE	Search...

Entry information

Entry name

NEIL1_MOUSE

Accession

Primary (citable) accession number: Q8K4Q6
Secondary accession number(s): Q80V58, Q9CYT9

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 7, 2004
Last sequence update:	January 23, 2007
Last modified:	April 3, 2013
This is version 90 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program