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Q8K4Q6

- NEIL1_MOUSE

UniProt

Q8K4Q6 - NEIL1_MOUSE

Protein

Endonuclease 8-like 1

Gene

Neil1

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 101 (01 Oct 2014)
      Sequence version 3 (23 Jan 2007)
      Previous versions | rss
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    Functioni

    Involved in base excision repair of DNA damaged by oxidation or by mutagenic agents. Acts as DNA glycosylase that recognizes and removes damaged bases. Has a preference for oxidized pyrimidines, such as thymine glycol, formamidopyrimidine (Fapy) and 5-hydroxyuracil. Has marginal activity towards 8-oxoguanine. Has AP (apurinic/apyrimidinic) lyase activity and introduces nicks in the DNA strand. Cleaves the DNA backbone by beta-delta elimination to generate a single-strand break at the site of the removed base with both 3'- and 5'-phosphates. Has DNA glycosylase/lyase activity towards mismatched uracil and thymine, in particular in U:C and T:C mismatches. Specifically binds 5-hydroxymethylcytosine (5hmC), suggesting that it acts as a specific reader of 5hmC.2 Publications

    Catalytic activityi

    Removes damaged bases from DNA, leaving an abasic site.
    The C-O-P bond 3' to the apurinic or apyrimidinic site in DNA is broken by a beta-elimination reaction, leaving a 3'-terminal unsaturated sugar and a product with a terminal 5'-phosphate.PROSITE-ProRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei2 – 21Schiff-base intermediate with DNACurated
    Active sitei3 – 31Proton donorCurated
    Active sitei54 – 541Proton donor; for beta-elimination activityCurated
    Binding sitei176 – 1761DNABy similarity

    GO - Molecular functioni

    1. damaged DNA binding Source: InterPro
    2. DNA-(apurinic or apyrimidinic site) lyase activity Source: UniProtKB-EC
    3. DNA N-glycosylase activity Source: MGI
    4. lyase activity Source: MGI
    5. zinc ion binding Source: InterPro

    GO - Biological processi

    1. base-excision repair Source: InterPro
    2. DNA repair Source: MGI
    3. negative regulation of nuclease activity Source: Ensembl
    4. nucleotide-excision repair Source: InterPro
    5. response to oxidative stress Source: Ensembl

    Keywords - Molecular functioni

    Glycosidase, Hydrolase, Lyase

    Keywords - Biological processi

    DNA damage, DNA repair

    Keywords - Ligandi

    DNA-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Endonuclease 8-like 1 (EC:3.2.2.-, EC:4.2.99.18)
    Alternative name(s):
    DNA glycosylase/AP lyase Neil1
    DNA-(apurinic or apyrimidinic site) lyase Neil1
    Endonuclease VIII-like 1
    Nei homolog 1
    Short name:
    NEH1
    Nei-like protein 1
    Gene namesi
    Name:Neil1
    Synonyms:Nei1
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 9

    Organism-specific databases

    MGIiMGI:1920024. Neil1.

    Subcellular locationi

    Cytoplasmcytoskeletonmicrotubule organizing centercentrosome. Nucleus. Chromosome
    Note: During mitosis, associates with centrosomes and condensed chromatin.By similarity

    GO - Cellular componenti

    1. chromosome Source: UniProtKB-SubCell
    2. cytoplasm Source: UniProtKB-KW
    3. microtubule organizing center Source: UniProtKB-SubCell
    4. nucleus Source: MGI

    Keywords - Cellular componenti

    Chromosome, Cytoplasm, Cytoskeleton, Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11RemovedBy similarity
    Chaini2 – 389388Endonuclease 8-like 1PRO_0000170906Add
    BLAST

    Proteomic databases

    PRIDEiQ8K4Q6.

    PTM databases

    PhosphoSiteiQ8K4Q6.

    Expressioni

    Tissue specificityi

    Detected in heart, spleen and lung.1 Publication

    Inductioni

    Up-regulated during S-phase.By similarity

    Gene expression databases

    BgeeiQ8K4Q6.
    CleanExiMM_NEIL1.
    GenevestigatoriQ8K4Q6.

    Interactioni

    Protein-protein interaction databases

    STRINGi10090.ENSMUSP00000034842.

    Structurei

    3D structure databases

    ProteinModelPortaliQ8K4Q6.
    SMRiQ8K4Q6. Positions 2-290.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the FPG family.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiNOG75119.
    GeneTreeiENSGT00390000016671.
    HOGENOMiHOG000067872.
    HOVERGENiHBG052592.
    InParanoidiQ8K4Q6.
    KOiK10567.
    OMAiHLASHFV.
    OrthoDBiEOG7H1JM2.
    PhylomeDBiQ8K4Q6.
    TreeFamiTF333272.

    Family and domain databases

    InterProiIPR015886. DNA_glyclase/AP_lyase_DNA-bd.
    IPR012319. DNA_glycosylase/AP_lyase_cat.
    IPR015371. Endonuclease-VIII_DNA-bd.
    IPR010979. Ribosomal_S13-like_H2TH.
    [Graphical view]
    PfamiPF01149. Fapy_DNA_glyco. 1 hit.
    PF06831. H2TH. 1 hit.
    PF09292. Neil1-DNA_bind. 1 hit.
    [Graphical view]
    SMARTiSM00898. Fapy_DNA_glyco. 1 hit.
    [Graphical view]
    SUPFAMiSSF46946. SSF46946. 1 hit.
    SSF81624. SSF81624. 1 hit.
    PROSITEiPS51068. FPG_CAT. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q8K4Q6-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MPEGPELHLA SHFVNETCKG LVFGGCVEKS SVSRNPEVPF ESSAYHISAL    50
    ARGKELRLTL SPLPGSQPPQ KPLSLVFRFG MSGSFQLVPA EALPRHAHLR 100
    FYTAPPAPRL ALCFVDIRRF GHWDPGGEWQ PGRGPCVLLE YERFRENVLR 150
    NLSDKAFDRP ICEALLDQRF FNGIGNYLRA EILYRLKIPP FEKARTVLEA 200
    LQQCRPSPEL TLSQKIKAKL QNPDLLELCH LVPKEVVQLG GKGYGPERGE 250
    EDFAAFRAWL RCYGVPGMSS LRDRHGRTIW FQGDPGPLAP KGGRSQKKKS 300
    QETQLGAEDR KEDLPLSSKS VSRMRRARKH PPKRIAQQSE GAGLQQNQET 350
    PTAPEKGKRR GQRASTGHRR RPKTIPDTRP REAGESSAS 389
    Length:389
    Mass (Da):43,586
    Last modified:January 23, 2007 - v3
    Checksum:iE3F1A21DDE2CB5AD
    GO
    Isoform 2 (identifier: Q8K4Q6-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         241-389: GKGYGPERGE...PREAGESSAS → EAWGGQDGRRPLP

    Note: No experimental confirmation available.

    Show »
    Length:253
    Mass (Da):28,453
    Checksum:i8BE7DE61651A166A
    GO

    Sequence cautioni

    The sequence BAB28790.1 differs from that shown. Reason: Frameshift at position 344.

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei241 – 389149GKGYG…ESSAS → EAWGGQDGRRPLP in isoform 2. 1 PublicationVSP_012207Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB079069 mRNA. Translation: BAC06477.1.
    AK013322 mRNA. Translation: BAB28790.1. Frameshift.
    BC043297 mRNA. Translation: AAH43297.1.
    CCDSiCCDS23217.1. [Q8K4Q6-1]
    RefSeqiNP_082623.1. NM_028347.2. [Q8K4Q6-1]
    XP_006511547.1. XM_006511484.1. [Q8K4Q6-1]
    XP_006511548.1. XM_006511485.1. [Q8K4Q6-1]
    XP_006511549.1. XM_006511486.1. [Q8K4Q6-1]
    UniGeneiMm.35749.
    Mm.488747.

    Genome annotation databases

    EnsembliENSMUST00000034842; ENSMUSP00000034842; ENSMUSG00000032298. [Q8K4Q6-1]
    GeneIDi72774.
    KEGGimmu:72774.
    UCSCiuc009puh.1. mouse. [Q8K4Q6-1]
    uc009puk.1. mouse. [Q8K4Q6-2]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB079069 mRNA. Translation: BAC06477.1 .
    AK013322 mRNA. Translation: BAB28790.1 . Frameshift.
    BC043297 mRNA. Translation: AAH43297.1 .
    CCDSi CCDS23217.1. [Q8K4Q6-1 ]
    RefSeqi NP_082623.1. NM_028347.2. [Q8K4Q6-1 ]
    XP_006511547.1. XM_006511484.1. [Q8K4Q6-1 ]
    XP_006511548.1. XM_006511485.1. [Q8K4Q6-1 ]
    XP_006511549.1. XM_006511486.1. [Q8K4Q6-1 ]
    UniGenei Mm.35749.
    Mm.488747.

    3D structure databases

    ProteinModelPortali Q8K4Q6.
    SMRi Q8K4Q6. Positions 2-290.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 10090.ENSMUSP00000034842.

    PTM databases

    PhosphoSitei Q8K4Q6.

    Proteomic databases

    PRIDEi Q8K4Q6.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000034842 ; ENSMUSP00000034842 ; ENSMUSG00000032298 . [Q8K4Q6-1 ]
    GeneIDi 72774.
    KEGGi mmu:72774.
    UCSCi uc009puh.1. mouse. [Q8K4Q6-1 ]
    uc009puk.1. mouse. [Q8K4Q6-2 ]

    Organism-specific databases

    CTDi 79661.
    MGIi MGI:1920024. Neil1.

    Phylogenomic databases

    eggNOGi NOG75119.
    GeneTreei ENSGT00390000016671.
    HOGENOMi HOG000067872.
    HOVERGENi HBG052592.
    InParanoidi Q8K4Q6.
    KOi K10567.
    OMAi HLASHFV.
    OrthoDBi EOG7H1JM2.
    PhylomeDBi Q8K4Q6.
    TreeFami TF333272.

    Miscellaneous databases

    NextBioi 336897.
    PROi Q8K4Q6.
    SOURCEi Search...

    Gene expression databases

    Bgeei Q8K4Q6.
    CleanExi MM_NEIL1.
    Genevestigatori Q8K4Q6.

    Family and domain databases

    InterProi IPR015886. DNA_glyclase/AP_lyase_DNA-bd.
    IPR012319. DNA_glycosylase/AP_lyase_cat.
    IPR015371. Endonuclease-VIII_DNA-bd.
    IPR010979. Ribosomal_S13-like_H2TH.
    [Graphical view ]
    Pfami PF01149. Fapy_DNA_glyco. 1 hit.
    PF06831. H2TH. 1 hit.
    PF09292. Neil1-DNA_bind. 1 hit.
    [Graphical view ]
    SMARTi SM00898. Fapy_DNA_glyco. 1 hit.
    [Graphical view ]
    SUPFAMi SSF46946. SSF46946. 1 hit.
    SSF81624. SSF81624. 1 hit.
    PROSITEi PS51068. FPG_CAT. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "A back-up glycosylase in Nth1 knock-out mice is a functional Nei (endonuclease VIII) homologue."
      Takao M., Kanno S., Kobayashi K., Zhang Q.-M., Yonei S., van der Horst G.T.J., Yasui A.
      J. Biol. Chem. 277:42205-42213(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, TISSUE SPECIFICITY.
      Tissue: Liver.
    2. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
      Strain: C57BL/6J.
      Tissue: Embryo.
    3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
      Strain: FVB/N.
      Tissue: Mammary gland.
    4. Cited for: FUNCTION.

    Entry informationi

    Entry nameiNEIL1_MOUSE
    AccessioniPrimary (citable) accession number: Q8K4Q6
    Secondary accession number(s): Q80V58, Q9CYT9
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 7, 2004
    Last sequence update: January 23, 2007
    Last modified: October 1, 2014
    This is version 101 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Multifunctional enzyme, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3