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Protein

E3 ubiquitin-protein ligase HECW1

Gene

Hecw1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

E3 ubiquitin-protein ligase that mediates ubiquitination and subsequent degradation of DVL1.By similarity1 Publication

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei1572 – 15721Glycyl thioester intermediatePROSITE-ProRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Ubl conjugation pathway

Enzyme and pathway databases

ReactomeiR-MMU-4641258. Degradation of DVL.
UniPathwayiUPA00143.

Names & Taxonomyi

Protein namesi
Recommended name:
E3 ubiquitin-protein ligase HECW1 (EC:6.3.2.-)
Alternative name(s):
HECT, C2 and WW domain-containing protein 1
NEDD4-like E3 ubiquitin-protein ligase 1
Short name:
mNEDL1
Gene namesi
Name:Hecw1
Synonyms:Kiaa0322, Nedl1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 13

Organism-specific databases

MGIiMGI:2444115. Hecw1.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 16041604E3 ubiquitin-protein ligase HECW1PRO_0000277666Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei871 – 8711PhosphoserineCombined sources
Modified residuei935 – 9351PhosphoserineCombined sources
Modified residuei937 – 9371PhosphoserineCombined sources

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ8K4P8.
PaxDbiQ8K4P8.
PeptideAtlasiQ8K4P8.
PRIDEiQ8K4P8.

PTM databases

iPTMnetiQ8K4P8.
PhosphoSiteiQ8K4P8.

Expressioni

Tissue specificityi

Predominantly expressed in neurons of the spinal cord.

Gene expression databases

BgeeiENSMUSG00000021301.
GenevisibleiQ8K4P8. MM.

Interactioni

Subunit structurei

Interacts with DVL1 and SSR3.By similarity

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000106145.

Structurei

3D structure databases

ProteinModelPortaliQ8K4P8.
SMRiQ8K4P8. Positions 77-181, 828-858, 946-1054.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini192 – 302111C2PROSITE-ProRule annotationAdd
BLAST
Domaini826 – 85934WW 1PROSITE-ProRule annotationAdd
BLAST
Domaini1016 – 104934WW 2PROSITE-ProRule annotationAdd
BLAST
Domaini1269 – 1604336HECTPROSITE-ProRule annotationAdd
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili871 – 89828Sequence analysisAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi407 – 524118Glu-richAdd
BLAST
Compositional biasi671 – 70333Cys-richAdd
BLAST

Sequence similaritiesi

Contains 1 C2 domain.PROSITE-ProRule annotation
Contains 1 HECT (E6AP-type E3 ubiquitin-protein ligase) domain.PROSITE-ProRule annotation
Contains 2 WW domains.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil, Repeat

Phylogenomic databases

eggNOGiKOG0940. Eukaryota.
COG5021. LUCA.
GeneTreeiENSGT00760000118966.
HOVERGENiHBG057414.
InParanoidiQ8K4P8.
KOiK12167.
OMAiTHYIRIH.
OrthoDBiEOG091G0A54.
TreeFamiTF313938.

Family and domain databases

Gene3Di2.60.40.150. 1 hit.
InterProiIPR000008. C2_dom.
IPR000569. HECT_dom.
IPR032348. HECW_N.
IPR001202. WW_dom.
[Graphical view]
PfamiPF00168. C2. 1 hit.
PF00632. HECT. 1 hit.
PF16562. HECW_N. 1 hit.
PF00397. WW. 1 hit.
[Graphical view]
SMARTiSM00239. C2. 1 hit.
SM00119. HECTc. 1 hit.
SM00456. WW. 2 hits.
[Graphical view]
SUPFAMiSSF49562. SSF49562. 1 hit.
SSF51045. SSF51045. 2 hits.
SSF56204. SSF56204. 1 hit.
PROSITEiPS50004. C2. 1 hit.
PS50237. HECT. 1 hit.
PS01159. WW_DOMAIN_1. 2 hits.
PS50020. WW_DOMAIN_2. 2 hits.
[Graphical view]

Sequences (4)i

Sequence statusi: Complete.

This entry describes 4 isoformsi produced by alternative splicing. AlignAdd to basket

Note: Named isoforms=2.
Isoform 1 (identifier: Q8K4P8-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MLLHLCSVKN LYQNRFLGLA AMASPSRNSQ SRRRCKEPLR YSYNPDQFHN
60 70 80 90 100
IDIRNGAHDA ITIPRSTSDT DLVTSDSRST LMVSSSYYSI GHSQDLVIHW
110 120 130 140 150
DIKEEVDAGD WIGMYLIGEV SSENFLDYKN RGVNGSHRGQ IIWKIDASSY
160 170 180 190 200
FVESETKICF KYYHGVSGAL RATTPSVTVK NSAAPIFKGI GSEETAQSQG
210 220 230 240 250
SRRLISFSLS DFQAMGLKKG MFFNPDPYLK ISIQPGKHSI FPALPHHGQE
260 270 280 290 300
RRSTIIGNTV NPIWQAEHFS FVSLPTDVLE IEVKDKFAKS RPIIKRFLGK
310 320 330 340 350
LSMPVQRLLE RHAIGDRVVS YTLGRRLPTD HVSGQLQFRF EITSSIHADD
360 370 380 390 400
EEISLSAEPE SSAETQDSIM NSMVGNSNGE PSGDATEFCK DAKPESPSEG
410 420 430 440 450
NGVNSSENQN QEHAGPVEEA AGAMEARDGS NVSEAPEEPG ELQDPEQHDT
460 470 480 490 500
QPTLSAEEVA EGLPLDEDSP SSLLPEENTA LGSKVEEETV PENGAREEEM
510 520 530 540 550
QKGKDEEEEE EDVSTLEQGE PGLELRVSVR KKSRPCSLPV SELETVIASA
560 570 580 590 600
CGDAETPRTH YIRIHTLLHS MPSAQRGSTT EEEDGLEEES TLKESSEKDG
610 620 630 640 650
LSEVDTIAAD PQSMEDGESD GATLCMAPSD CSGGHFSSLS KGIGAGQDGE
660 670 680 690 700
AHPSTGSESD SSPQQGADHS CEGCDASCCS PSCYSTSCYS SSCYSSSCYS
710 720 730 740 750
SSCYNGNNRF ASHTRFSSVD SAKISESTVF SSQEDEEEEN SAFESVPDSV
760 770 780 790 800
QSPELDPEST NGAGPWQDEL AAPGGNAARS TEGLESPMAG PSNRREGECP
810 820 830 840 850
ILHNSQPISQ LPSLRPEHHH YPTIDEPLPP NWEARIDSHG RVFYVDHINR
860 870 880 890 900
TTTWQRPSMA PTPDGMIRSG SVHQMEQLNR RYQNIQRTMA TERAEEDSGN
910 920 930 940 950
QNSEQIPDGG GGGGGGSDSE AESSQSSLDL RREGSLSPVN SQKVTLLLQS
960 970 980 990 1000
PAVKFITNPE FFTVLHANYS AYRVFTSSTC LKHMILKVRR DARNFERYQH
1010 1020 1030 1040 1050
NRDLVNFINM FADTRLELPR GWEIKTDHQG KSFFVDHNSR ATTFIDPRIP
1060 1070 1080 1090 1100
LQNGRLPNHL THRQHLQRLR SYSAGEASEV SRNRGASLLA RPGHSLIAAI
1110 1120 1130 1140 1150
RSQHQHESLP LAYNDKIVAF LRQPNIFEML QERQPSLARN HTLREKIHYI
1160 1170 1180 1190 1200
RTEGNHGLDK LSCDADLVIL LSLFEEEIMS YVPLQSAFHP GYSFSPRCSP
1210 1220 1230 1240 1250
CSSPQNSPGL QRASARAPSP YRRDFEAKLR NFYRKLEAKG FGQGPGKIKL
1260 1270 1280 1290 1300
IIRRDHLLEG TFNQVMAYSR KELQRNKLYI TFVGEEGLDY SGPSREFFFL
1310 1320 1330 1340 1350
LSQELFNPYY GLFEYSANDT YTVQISPMSA FVENYLEWFR FSGRILGLAL
1360 1370 1380 1390 1400
IHQYLLDAFF TRPFYKGLLK LPCDLSDLEY LDEEFHQSLQ WMKDNNITDI
1410 1420 1430 1440 1450
LDLTFTVNEE VFGQVTEREL KSGGANTQVT EKNKKEYIER MVKWRVERGV
1460 1470 1480 1490 1500
VQQTEALLRG FYEVVDSRLV SVFDARELEL VIAGTAEIDL NDWRNNTEYR
1510 1520 1530 1540 1550
GGYHDGHLVI RWFWAAVERF NNEQRLRLLQ FVTGTSSVPY EGFAALRGSN
1560 1570 1580 1590 1600
GLRRFCIEKW GKITSLPRAH TCFNRLDLPP YPSYSMLYEK LLTAVEETST

FGLE
Length:1,604
Mass (Da):179,467
Last modified:July 27, 2011 - v3
Checksum:i0643EE0129A08E0D
GO
Isoform 2 (identifier: Q8K4P8-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     782-795: Missing.

Note: No experimental confirmation available.
Show »
Length:1,590
Mass (Da):177,984
Checksum:i51C7D0DA9432CED2
GO
Isoform 3 (identifier: Q8K4P8-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-8: MLLHLCSV → MICAQLEAA
     316-1604: Missing.

Note: No experimental confirmation available.
Show »
Length:316
Mass (Da):35,504
Checksum:iE3336039B30263B4
GO
Isoform 4 (identifier: Q8K4P8-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     316-1604: Missing.

Note: No experimental confirmation available.
Show »
Length:315
Mass (Da):35,470
Checksum:i2D5E366B748ED3E2
GO

Sequence cautioni

The sequence BAC28516 differs from that shown. Reason: Erroneous initiation. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti167 – 1671S → R in BAB97389 (PubMed:14684739).Curated
Sequence conflicti823 – 8231T → A in BAB97389 (PubMed:14684739).Curated
Sequence conflicti1036 – 10361D → G in BAB97389 (PubMed:14684739).Curated
Sequence conflicti1248 – 12481I → V in BAB97389 (PubMed:14684739).Curated
Sequence conflicti1356 – 13561L → P in BAB97389 (PubMed:14684739).Curated
Sequence conflicti1538 – 15381V → M in BAB97389 (PubMed:14684739).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 88MLLHLCSV → MICAQLEAA in isoform 3. 1 PublicationVSP_023074
Alternative sequencei316 – 16041289Missing in isoform 3 and isoform 4. 1 PublicationVSP_023075Add
BLAST
Alternative sequencei782 – 79514Missing in isoform 2. 1 PublicationVSP_023076Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK033922 mRNA. Translation: BAC28516.1. Different initiation.
AK047678 mRNA. Translation: BAC33122.1.
AK051569 mRNA. Translation: BAC34677.1.
AK053694 mRNA. Translation: BAC35477.1.
AC092710 Genomic DNA. No translation available.
AC154511 Genomic DNA. No translation available.
AC154615 Genomic DNA. No translation available.
AC161249 Genomic DNA. No translation available.
CT025604 Genomic DNA. No translation available.
AB083710 mRNA. Translation: BAB97389.1.
AK172932 mRNA. Translation: BAD32210.1.
CCDSiCCDS49207.1. [Q8K4P8-1]
RefSeqiNP_001074817.3. NM_001081348.3. [Q8K4P8-1]
XP_006516873.1. XM_006516810.2. [Q8K4P8-1]
XP_006516874.1. XM_006516811.1. [Q8K4P8-1]
XP_006516875.1. XM_006516812.2. [Q8K4P8-1]
UniGeneiMm.125298.

Genome annotation databases

EnsembliENSMUST00000110516; ENSMUSP00000106145; ENSMUSG00000021301. [Q8K4P8-1]
GeneIDi94253.
KEGGimmu:94253.
UCSCiuc007pnd.2. mouse. [Q8K4P8-1]
uc007png.2. mouse. [Q8K4P8-4]
uc007pnh.2. mouse. [Q8K4P8-3]
uc011ywk.1. mouse. [Q8K4P8-2]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK033922 mRNA. Translation: BAC28516.1. Different initiation.
AK047678 mRNA. Translation: BAC33122.1.
AK051569 mRNA. Translation: BAC34677.1.
AK053694 mRNA. Translation: BAC35477.1.
AC092710 Genomic DNA. No translation available.
AC154511 Genomic DNA. No translation available.
AC154615 Genomic DNA. No translation available.
AC161249 Genomic DNA. No translation available.
CT025604 Genomic DNA. No translation available.
AB083710 mRNA. Translation: BAB97389.1.
AK172932 mRNA. Translation: BAD32210.1.
CCDSiCCDS49207.1. [Q8K4P8-1]
RefSeqiNP_001074817.3. NM_001081348.3. [Q8K4P8-1]
XP_006516873.1. XM_006516810.2. [Q8K4P8-1]
XP_006516874.1. XM_006516811.1. [Q8K4P8-1]
XP_006516875.1. XM_006516812.2. [Q8K4P8-1]
UniGeneiMm.125298.

3D structure databases

ProteinModelPortaliQ8K4P8.
SMRiQ8K4P8. Positions 77-181, 828-858, 946-1054.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000106145.

PTM databases

iPTMnetiQ8K4P8.
PhosphoSiteiQ8K4P8.

Proteomic databases

MaxQBiQ8K4P8.
PaxDbiQ8K4P8.
PeptideAtlasiQ8K4P8.
PRIDEiQ8K4P8.

Protocols and materials databases

DNASUi94253.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000110516; ENSMUSP00000106145; ENSMUSG00000021301. [Q8K4P8-1]
GeneIDi94253.
KEGGimmu:94253.
UCSCiuc007pnd.2. mouse. [Q8K4P8-1]
uc007png.2. mouse. [Q8K4P8-4]
uc007pnh.2. mouse. [Q8K4P8-3]
uc011ywk.1. mouse. [Q8K4P8-2]

Organism-specific databases

CTDi23072.
MGIiMGI:2444115. Hecw1.
RougeiSearch...

Phylogenomic databases

eggNOGiKOG0940. Eukaryota.
COG5021. LUCA.
GeneTreeiENSGT00760000118966.
HOVERGENiHBG057414.
InParanoidiQ8K4P8.
KOiK12167.
OMAiTHYIRIH.
OrthoDBiEOG091G0A54.
TreeFamiTF313938.

Enzyme and pathway databases

UniPathwayiUPA00143.
ReactomeiR-MMU-4641258. Degradation of DVL.

Miscellaneous databases

PROiQ8K4P8.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000021301.
GenevisibleiQ8K4P8. MM.

Family and domain databases

Gene3Di2.60.40.150. 1 hit.
InterProiIPR000008. C2_dom.
IPR000569. HECT_dom.
IPR032348. HECW_N.
IPR001202. WW_dom.
[Graphical view]
PfamiPF00168. C2. 1 hit.
PF00632. HECT. 1 hit.
PF16562. HECW_N. 1 hit.
PF00397. WW. 1 hit.
[Graphical view]
SMARTiSM00239. C2. 1 hit.
SM00119. HECTc. 1 hit.
SM00456. WW. 2 hits.
[Graphical view]
SUPFAMiSSF49562. SSF49562. 1 hit.
SSF51045. SSF51045. 2 hits.
SSF56204. SSF56204. 1 hit.
PROSITEiPS50004. C2. 1 hit.
PS50237. HECT. 1 hit.
PS01159. WW_DOMAIN_1. 2 hits.
PS50020. WW_DOMAIN_2. 2 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiHECW1_MOUSE
AccessioniPrimary (citable) accession number: Q8K4P8
Secondary accession number(s): D3YZ67
, Q6A086, Q8BIA6, Q8BKC2, Q8BKL3, Q8BKZ8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 20, 2007
Last sequence update: July 27, 2011
Last modified: September 7, 2016
This is version 106 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.