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Protein

Oxysterol-binding protein-related protein 1

Gene

Osbpl1a

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Binds phospholipids; exhibits strong binding to phosphatidic acid and weak binding to phosphatidylinositol 3-phosphate. Stabilizes GTP-bound RAB7A on late endosomes/lysosomes and alters functional properties of late endocytic compartments via its interaction with RAB7A. Binds 25-hydroxycholesterol and cholesterol.By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Lipid transport, Transport

Keywords - Ligandi

Lipid-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Oxysterol-binding protein-related protein 1
Short name:
ORP-1
Short name:
OSBP-related protein 1
Gene namesi
Name:Osbpl1a
Synonyms:Orp1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi628888. Osbpl1a.

Subcellular locationi

  • Late endosome By similarity

  • Note: Co-localizes with RAB7A, RAB9A and LAMP1 in late endosomes.By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Endosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 950950Oxysterol-binding protein-related protein 1PRO_0000228846Add
BLAST

Proteomic databases

PaxDbiQ8K4M9.
PRIDEiQ8K4M9.

PTM databases

PhosphoSiteiQ8K4M9.

Expressioni

Tissue specificityi

Detected in prostate and liver.1 Publication

Interactioni

Subunit structurei

Interacts with VAPA. Interacts with the GTP-bound form of RAB7A (By similarity). Interacts with OAS1B (By similarity).By similarity

Protein-protein interaction databases

DIPiDIP-48447N.
STRINGi10116.ENSRNOP00000065976.

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1Z9OX-ray1.90G/H/I/J/K/L472-481[»]
ProteinModelPortaliQ8K4M9.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ8K4M9.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati47 – 7630ANK 1Add
BLAST
Repeati80 – 10930ANK 2Add
BLAST
Repeati175 – 20430ANK 3Add
BLAST
Domaini235 – 334100PHPROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 237237Interaction with RAB7ABy similarityAdd
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili430 – 46334Sequence analysisAdd
BLAST
Coiled coili877 – 91337Sequence analysisAdd
BLAST

Sequence similaritiesi

Belongs to the OSBP family.Curated
Contains 3 ANK repeats.PROSITE-ProRule annotation
Contains 1 PH domain.PROSITE-ProRule annotation

Keywords - Domaini

ANK repeat, Coiled coil, Repeat

Phylogenomic databases

eggNOGiKOG1737. Eukaryota.
ENOG410XP9E. LUCA.
HOVERGENiHBG082087.
InParanoidiQ8K4M9.
KOiK20174.
PhylomeDBiQ8K4M9.

Family and domain databases

Gene3Di1.25.40.20. 1 hit.
2.30.29.30. 1 hit.
InterProiIPR002110. Ankyrin_rpt.
IPR020683. Ankyrin_rpt-contain_dom.
IPR000648. Oxysterol-bd.
IPR018494. Oxysterol-bd_CS.
IPR011993. PH_dom-like.
IPR001849. PH_domain.
[Graphical view]
PANTHERiPTHR10972. PTHR10972. 2 hits.
PfamiPF12796. Ank_2. 1 hit.
PF01237. Oxysterol_BP. 1 hit.
[Graphical view]
PRINTSiPR01415. ANKYRIN.
SMARTiSM00248. ANK. 3 hits.
SM00233. PH. 1 hit.
[Graphical view]
SUPFAMiSSF48403. SSF48403. 1 hit.
SSF50729. SSF50729. 1 hit.
PROSITEiPS50297. ANK_REP_REGION. 1 hit.
PS50088. ANK_REPEAT. 3 hits.
PS01013. OSBP. 1 hit.
PS50003. PH_DOMAIN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8K4M9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNTEAEQQLL HHARNGNAEE VRKLLEAMAR AEVVADIDCK GRSKSNLGWT
60 70 80 90 100
PLHLACYFGH KQVVQDLLKA GAKVNMLNDM GDTPLHRAAF TGRKELVMLL
110 120 130 140 150
LEYNADTTVV NGSGQTAKEA THDKEIRQML EAVERTQQRK LEELLLGAAR
160 170 180 190 200
EGRTAEVSAL LSRPNPPDVN CSDQLGNTPL HCAAYRAHKQ CVLKLLRSGA
210 220 230 240 250
DPSLKNKNDQ KPLDLAHGTE MKHILVGNKV VHKALKRFEG PLWKSSRFFG
260 270 280 290 300
WKLFWVVLEH GVLSWYRKQP DAVHNSYRQG CKHLTQAVCT VKPTDGCLFS
310 320 330 340 350
VRCFDDTVHG FRVPKNSLQQ SREKWLEAIE EHSAYSTHYC SQDQVTDDEE
360 370 380 390 400
EDVASAMDLK ESLARAQTCQ QRLDREIYNF LKMIKECDVA KDMLPSFLQK
410 420 430 440 450
ADVLSEASRE TCVALSDCLN LFTKQEGVRN FKLEQEQEKN KILSEALETL
460 470 480 490 500
ATEHHELERS LVEGSPPVSI LSEDEFYDAL SGSESEGSLT CLEAVTARAF
510 520 530 540 550
EENEVPANSG KHRMSEGKDC GGGDALSNGI KKHRTSLPSP MFSRNDFSIW
560 570 580 590 600
SILRKCIGME LSKITMPVIF NEPLSFLQRL TEYMEHTYLI HKASSFSDPV
610 620 630 640 650
ERMQCVAAFA VSAVASQWER TGKPFNPLLG ETYELVRDDL GFRLISEQVS
660 670 680 690 700
HHPPISAFHA EGLNNDFIFH GSIYPKLKFW GKSVEAEPKG TITLELLEHN
710 720 730 740 750
EAYTWTNPTC CVHNIIVGKL WIEQYGNVEI INHKTGDKCV LNFKPCGLFG
760 770 780 790 800
KELHKVEGYI QDKSKKKLCA LYGKWTECLY SVDPATFDAY KKNDKKNTEE
810 820 830 840 850
KKTSKQASTS EESDEMPVPD SESVFVIPGS ALLWRIAPRP PNSAQMYNFT
860 870 880 890 900
SFAMVLNEVD KEMETVIPKT DCRLRPDIRA MENGEIDQAS EEKKRLEEKQ
910 920 930 940 950
RAARKNRSKS EEDWKTRWFH QGPNPYSGAQ DWIYSGSYWD RNYFNLPDIY
Length:950
Mass (Da):107,765
Last modified:October 1, 2002 - v1
Checksum:iCA312E8C7326B61F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB089316 mRNA. Translation: BAC07227.1.
RefSeqiNP_742020.1. NM_172023.1.
UniGeneiRn.178664.

Genome annotation databases

GeneIDi259221.
KEGGirno:259221.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB089316 mRNA. Translation: BAC07227.1.
RefSeqiNP_742020.1. NM_172023.1.
UniGeneiRn.178664.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1Z9OX-ray1.90G/H/I/J/K/L472-481[»]
ProteinModelPortaliQ8K4M9.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-48447N.
STRINGi10116.ENSRNOP00000065976.

PTM databases

PhosphoSiteiQ8K4M9.

Proteomic databases

PaxDbiQ8K4M9.
PRIDEiQ8K4M9.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi259221.
KEGGirno:259221.

Organism-specific databases

CTDi114876.
RGDi628888. Osbpl1a.

Phylogenomic databases

eggNOGiKOG1737. Eukaryota.
ENOG410XP9E. LUCA.
HOVERGENiHBG082087.
InParanoidiQ8K4M9.
KOiK20174.
PhylomeDBiQ8K4M9.

Miscellaneous databases

EvolutionaryTraceiQ8K4M9.
PROiQ8K4M9.

Family and domain databases

Gene3Di1.25.40.20. 1 hit.
2.30.29.30. 1 hit.
InterProiIPR002110. Ankyrin_rpt.
IPR020683. Ankyrin_rpt-contain_dom.
IPR000648. Oxysterol-bd.
IPR018494. Oxysterol-bd_CS.
IPR011993. PH_dom-like.
IPR001849. PH_domain.
[Graphical view]
PANTHERiPTHR10972. PTHR10972. 2 hits.
PfamiPF12796. Ank_2. 1 hit.
PF01237. Oxysterol_BP. 1 hit.
[Graphical view]
PRINTSiPR01415. ANKYRIN.
SMARTiSM00248. ANK. 3 hits.
SM00233. PH. 1 hit.
[Graphical view]
SUPFAMiSSF48403. SSF48403. 1 hit.
SSF50729. SSF50729. 1 hit.
PROSITEiPS50297. ANK_REP_REGION. 1 hit.
PS50088. ANK_REPEAT. 3 hits.
PS01013. OSBP. 1 hit.
PS50003. PH_DOMAIN. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Androgen-regulated expression of a novel member of the aldo-keto reductase superfamily in regrowing rat prostate."
    Nishi N., Shoji H., Miyanaka H., Nakamura T.
    Endocrinology 141:3194-3199(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
    Tissue: Liver.
  2. Cited for: X-RAY CRYSTALLOGRAPHY (1.9 ANGSTROMS) OF 472-481 IN COMPLEX WITH VAPA.

Entry informationi

Entry nameiOSBL1_RAT
AccessioniPrimary (citable) accession number: Q8K4M9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 21, 2006
Last sequence update: October 1, 2002
Last modified: July 6, 2016
This is version 74 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.