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Q8K4L3 (SVIL_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 102. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Supervillin
Alternative name(s):
Archvillin
p205/p250
Gene names
Name:Svil
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length2170 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Forms a high-affinity link between the actin cytoskeleton and the membrane. Isoform 1 (archvillin) is among the first costameric proteins to assemble during myogenesis and it contributes to myogenic membrane structure and differentiation. Appears to be involved in myosin II assembly. May modulate myosin II regulation through MLCK during cell spreading, an initial step in cell migration. May play a role in invadopodial function. Isoform 2 may be involved in modulation of focal adhesions. Supervillin-mediated down-regulation of focal adhesions involves binding to TRIP6 By similarity.

Subunit structure

Associates with F-actin. Interacts with NEB. Interacts with MYH9. Interacts with MYLK. Isoform 2 interacts with TRIP6. Isoform 2 interacts with DYNLT1 By similarity.

Subcellular location

Cell membrane; Peripheral membrane protein; Cytoplasmic side. Cytoplasmcytoskeleton. Cell projectioninvadopodium By similarity. Cell projectionpodosome By similarity. Note: Tightly associated with both actin filaments and plasma membranes.

Domain

As opposed to other villin-type headpiece domains, supervillin HP (SVHP) doesn't bind F-actin due to the absence of a conformationally flexible region (V-loop) By similarity.

Sequence similarities

Belongs to the villin/gelsolin family.

Contains 5 gelsolin-like repeats.

Contains 1 HP (headpiece) domain.

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Select]
Isoform 1 (identifier: Q8K4L3-1)

Also known as: Archvillin;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q8K4L3-2)

Also known as: Supervillin;

The sequence of this isoform is not available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 21702170Supervillin
PRO_0000218741

Regions

Repeat1397 – 1496100Gelsolin-like 1
Repeat1516 – 1638123Gelsolin-like 2
Repeat1708 – 1818111Gelsolin-like 3
Repeat1837 – 1938102Gelsolin-like 4
Repeat1971 – 2078108Gelsolin-like 5
Domain2107 – 217064HP
Region1 – 167167Interaction with MYLK By similarity
Region1375 – 1643269Interaction with NEB By similarity

Amino acid modifications

Modified residue501Phosphoserine By similarity
Modified residue2271Phosphoserine Ref.4
Modified residue6661Phosphoserine By similarity
Modified residue8111Phosphothreonine By similarity
Modified residue8571Phosphoserine Ref.2
Modified residue8771Phosphoserine By similarity
Modified residue8811Phosphoserine By similarity
Modified residue9601Phosphoserine Ref.2 Ref.3
Modified residue10771Phosphoserine By similarity
Modified residue11811Phosphoserine By similarity
Modified residue11861Phosphothreonine By similarity
Modified residue12781Phosphoserine By similarity

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 (Archvillin) [UniParc].

Last modified October 1, 2002. Version 1.
Checksum: AEAB710184FB71C6

FASTA2,170243,162
        10         20         30         40         50         60 
MKRKERIARR LEGIENDSQP ILLQSCTGLV THRLLEEDTP RYMRATDPAS PHIGRSKEEE 

        70         80         90        100        110        120 
DTPGSSLEKQ TPSKYCIETS GIHSSGSMDT HSLESKAERI ARYKAERRRQ LAEKYGLTLD 

       130        140        150        160        170        180 
PEADSEYLSR YAKSRKDPDV TERRGKSDKQ EEQSKDANSR HSRTESGPRT SLVASQDCTP 

       190        200        210        220        230        240 
LGSNMSDQEQ LLNVENQRRV QDPPLGEDGS SAFFSERSIS FPEVPRSPKQ IPSSPLQQPA 

       250        260        270        280        290        300 
SPNHPGDSPL PTEARASTGK PTHEWFLQRD SEGDTPSLIN WPSRVKVREK LVKEESARSS 

       310        320        330        340        350        360 
PELTSESLTQ RRQQPAPAHF LPIQSESSTF DRVTSKAVSS LQPSQSGVLP TDPVHAIKLV 

       370        380        390        400        410        420 
TMDTPESTSE FSWVGSATPK VIKSTTLKIL EGGSRDAPVL HICESKAEDW LSPEPLERSP 

       430        440        450        460        470        480 
KSLLTSEDDR LVRGHKDPSG NKDLDKAIIC SIDVESERER QVQHLPTQRT GRSEMLLYVQ 

       490        500        510        520        530        540 
SGPVSQDATL TSHTKEASPK KRKVLARSLS DYTGPPQLQV PRHKDEAPSQ ELELQSSRAE 

       550        560        570        580        590        600 
GPGAEASVLD TRVSVAQLRN IFMESTRASK KPELQSRVER SAEGIGLPME RERGSRKPRR 

       610        620        630        640        650        660 
YLSPGESRKT SERFRTQPIT SAERKESDRY PSGSEIPVVE DEEKVDERAK LSVAAKRLLF 

       670        680        690        700        710        720 
REMEKSFDEH TVPKRHSRNA AVEQRLRRLQ DRSHTQPITT EEVVIAATEP IPASCSGVTH 

       730        740        750        760        770        780 
PVTARLPSPT VARSSVQPAR LQASAHQKAL ARDQANEGRE SAEPGEPDSS TLSLAEKLAL 

       790        800        810        820        830        840 
FNKLSQPVSK AISTRNRIDV RQRRMNARYQ TQPVTLGEVE QVQSGKLISF SPTVNTSVSI 

       850        860        870        880        890        900 
MASAVAPTYA GDLRKLSVDN NTSATDYKSP PAENSDSPVR SILKPQAWRP LVEHSGSKGM 

       910        920        930        940        950        960 
PGESGKTESK NALTVAAEDS GVQTRGAFEE EEEPSYPILG RVREGDGQKE PKHVVLRRGS 

       970        980        990       1000       1010       1020 
LELGNPSAAH LGDELKEVST AKSSLQENLD LKDKQASEEN TDVETVMRKF SLKEFGETTS 

      1030       1040       1050       1060       1070       1080 
EQTEVAARKA SVQMATPGAW KQQESSEQLA EKLFKNPCAM FASGEVKVPV GDSFLDSPSK 

      1090       1100       1110       1120       1130       1140 
TMSIKERLAL LKKSGEEDWK NRLIRKQEYG KATGGLHTQE VEQSLKKKRV TESRESQMTI 

      1150       1160       1170       1180       1190       1200 
EERKHLITVR EEAWKTKGRG AANDSTQFTV AGRMVKKGLA SPTSITPISS PLCSKSRGTT 

      1210       1220       1230       1240       1250       1260 
PVSKPLEDIE ARPDMQLESD LKLDRLETFL RRLNNKVAGI QETVLTVTGK SVKEVMKLDD 

      1270       1280       1290       1300       1310       1320 
DETFAKFYRS VDHSIPRSPV ELEEDFDVIF DPYAPKLTSS VAEHKRQVRP KRRVQASKNP 

      1330       1340       1350       1360       1370       1380 
LKLLAARDDL LQEYTEQRLN VAFMESKRMK VEKMSSNSNF SEVTLAGLAS RENFSNINLR 

      1390       1400       1410       1420       1430       1440 
SVNLMEQNSN NSAMPYKKLM LLQIKGRRHV QTRLVEPRAS SLNSGDCFLL LSPQYCFLWV 

      1450       1460       1470       1480       1490       1500 
GEFSNVIEKA KASELATLIQ TKRELGCRAT YIQTIEEGIN THTHAAKDFW KLLGGQTSYQ 

      1510       1520       1530       1540       1550       1560 
SAGDPKEDEL YETAIIETNC VYRLTDDKLV PDDDYWGKIP KCSLLQSKEV LVFDFGSEVY 

      1570       1580       1590       1600       1610       1620 
VWHGKEVTLA QRKIAFQLAK HLWNGTFDYE NCDINPLDPG ECNPLIPRKG QGRPDWAIFG 

      1630       1640       1650       1660       1670       1680 
RVTEHNETIL FKEKFLDWTE LKRPTEKNSG EVVQQKDDPR ADVKPYDVTR MVATPQITAG 

      1690       1700       1710       1720       1730       1740 
TILDGVNVGR GYGLVEGDDR RQFEIATVSV DVWHILEFDY SRLPRQSIGQ FHEGDAYVVK 

      1750       1760       1770       1780       1790       1800 
WKYMASTAVG SRQKGEHLVR VAGKEKCVYF FWQGRHSTVS EKGTSALMTV ELDEERGAQV 

      1810       1820       1830       1840       1850       1860 
QVLQGKEPPC FLQCFQGGMV VHSGRREEEE ENVQSEWRLY CVRGEVPMEG NLLEVACHCS 

      1870       1880       1890       1900       1910       1920 
SLRSRTSMVV LNINKALIYL WHGCKAQGHT KEVGRTAANK IKEECPLEAG LHSSSNVTIH 

      1930       1940       1950       1960       1970       1980 
ECDEGSEPLG FWDALGRRDR KAYDCMLQDP GSFNFAPRLF ILSSSSGDFS ATEFVYPAQA 

      1990       2000       2010       2020       2030       2040 
PSAVSSMPFL QEDLYSAPQP ALFLVDNHHE VYLWQGWWPT ENKITGSARI RWASDRKSAM 

      2050       2060       2070       2080       2090       2100 
ETVLQYCRGK NLKRPPPKSY LIHAGLEPLT FTNMFPSWEH REDIAEITEM DTEVSNQITL 

      2110       2120       2130       2140       2150       2160 
VEDVLAKLCK TIYPLADLLA RPLPEGVDPL KLEIYLTDED FEFALDMSRD EFNALPTWKQ 

      2170 
VNLKKSKGLF 

« Hide

Isoform 2 (Supervillin) (Sequence not available).

References

« Hide 'large scale' references
[1]"Archvillin, a muscle-specific isoform of supervillin, is an early expressed component of the costameric membrane skeleton."
Oh S.W., Pope R.K., Smith K.P., Crowley J.L., Nebl T., Lawrence J.B., Luna E.J.
J. Cell Sci. 116:2261-2275(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Skeletal muscle.
[2]"Large-scale phosphorylation analysis of mouse liver."
Villen J., Beausoleil S.A., Gerber S.A., Gygi S.P.
Proc. Natl. Acad. Sci. U.S.A. 104:1488-1493(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-857 AND SER-960, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Liver.
[3]"The phagosomal proteome in interferon-gamma-activated macrophages."
Trost M., English L., Lemieux S., Courcelles M., Desjardins M., Thibault P.
Immunity 30:143-154(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-960, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[4]"Large scale localization of protein phosphorylation by use of electron capture dissociation mass spectrometry."
Sweet S.M., Bailey C.M., Cunningham D.L., Heath J.K., Cooper H.J.
Mol. Cell. Proteomics 8:904-912(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-227, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Embryonic fibroblast.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF317422 mRNA. Translation: AAM89518.1.
CCDSCCDS37720.1. [Q8K4L3-1]
RefSeqNP_694793.1. NM_153153.3. [Q8K4L3-1]
UniGeneMm.136791.

3D structure databases

ProteinModelPortalQ8K4L3.
SMRQ8K4L3. Positions 1398-1640, 1712-2079, 2104-2170.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid230358. 1 interaction.
IntActQ8K4L3. 1 interaction.
MINTMINT-4136191.

PTM databases

PhosphoSiteQ8K4L3.

Proteomic databases

MaxQBQ8K4L3.
PaxDbQ8K4L3.
PRIDEQ8K4L3.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000025079; ENSMUSP00000025079; ENSMUSG00000024236. [Q8K4L3-1]
ENSMUST00000126977; ENSMUSP00000115078; ENSMUSG00000024236. [Q8K4L3-1]
ENSMUST00000140448; ENSMUSP00000119803; ENSMUSG00000024236. [Q8K4L3-1]
GeneID225115.
KEGGmmu:225115.
UCSCuc008dyr.2. mouse. [Q8K4L3-1]

Organism-specific databases

CTD6840.
MGIMGI:2147319. Svil.

Phylogenomic databases

eggNOGNOG324767.
GeneTreeENSGT00660000095502.
HOGENOMHOG000006939.
HOVERGENHBG052980.
InParanoidQ8K4L3.
KOK10369.
OMARSHTQPI.
OrthoDBEOG7WX07J.
PhylomeDBQ8K4L3.
TreeFamTF316081.

Gene expression databases

ArrayExpressQ8K4L3.
BgeeQ8K4L3.
CleanExMM_SVIL.
GenevestigatorQ8K4L3.

Family and domain databases

Gene3D1.10.950.10. 1 hit.
3.40.20.10. 5 hits.
InterProIPR029006. ADF-H/Gelsolin-like_dom.
IPR007123. Gelsolin-like_dom.
IPR015628. Supervillin.
IPR007122. Villin/Gelsolin.
IPR003128. Villin_headpiece.
[Graphical view]
PANTHERPTHR11977. PTHR11977. 1 hit.
PTHR11977:SF28. PTHR11977:SF28. 1 hit.
PfamPF00626. Gelsolin. 1 hit.
PF02209. VHP. 1 hit.
[Graphical view]
PRINTSPR00597. GELSOLIN.
SMARTSM00262. GEL. 5 hits.
SM00153. VHP. 1 hit.
[Graphical view]
SUPFAMSSF47050. SSF47050. 1 hit.
PROSITEPS51089. HP. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSSVIL. mouse.
NextBio377534.
PROQ8K4L3.
SOURCESearch...

Entry information

Entry nameSVIL_MOUSE
AccessionPrimary (citable) accession number: Q8K4L3
Entry history
Integrated into UniProtKB/Swiss-Prot: January 4, 2005
Last sequence update: October 1, 2002
Last modified: July 9, 2014
This is version 102 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot