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Protein

Supervillin

Gene

Svil

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Forms a high-affinity link between the actin cytoskeleton and the membrane. Isoform 1 (archvillin) is among the first costameric proteins to assemble during myogenesis and it contributes to myogenic membrane structure and differentiation. Appears to be involved in myosin II assembly. May modulate myosin II regulation through MLCK during cell spreading, an initial step in cell migration. May play a role in invadopodial function. Isoform 2 may be involved in modulation of focal adhesions. Supervillin-mediated down-regulation of focal adhesions involves binding to TRIP6. Plays a role in cytokinesis through KIF14 interaction (By similarity).By similarity

GO - Molecular functioni

  1. actin filament binding Source: MGI

GO - Biological processi

  1. cytoskeleton organization Source: InterPro
  2. positive regulation of cytokinesis Source: UniProtKB
  3. skeletal muscle tissue development Source: MGI
Complete GO annotation...

Keywords - Ligandi

Actin-binding, Calcium

Names & Taxonomyi

Protein namesi
Recommended name:
Supervillin
Alternative name(s):
Archvillin
p205/p250
Gene namesi
Name:Svil
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 18

Organism-specific databases

MGIiMGI:2147319. Svil.

Subcellular locationi

Cell membrane; Peripheral membrane protein; Cytoplasmic side. Cytoplasmcytoskeleton. Cell projectioninvadopodium By similarity. Cell projectionpodosome By similarity. Midbody By similarity. Cleavage furrow By similarity
Note: Tightly associated with both actin filaments and plasma membranes.

GO - Cellular componenti

  1. actin cytoskeleton Source: MGI
  2. cleavage furrow Source: UniProtKB
  3. costamere Source: MGI
  4. cytoplasm Source: MGI
  5. focal adhesion Source: MGI
  6. invadopodium Source: UniProtKB-SubCell
  7. microtubule minus-end Source: UniProtKB
  8. midbody Source: UniProtKB
  9. nucleus Source: MGI
  10. plasma membrane Source: MGI
  11. podosome Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cell membrane, Cell projection, Cytoplasm, Cytoskeleton, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 21702170SupervillinPRO_0000218741Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei50 – 501PhosphoserineBy similarity
Modified residuei227 – 2271Phosphoserine1 Publication
Modified residuei666 – 6661PhosphoserineBy similarity
Modified residuei811 – 8111PhosphothreonineBy similarity
Modified residuei857 – 8571Phosphoserine1 Publication
Modified residuei877 – 8771PhosphoserineBy similarity
Modified residuei881 – 8811PhosphoserineBy similarity
Modified residuei960 – 9601Phosphoserine2 Publications
Modified residuei1077 – 10771PhosphoserineBy similarity
Modified residuei1181 – 11811PhosphoserineBy similarity
Modified residuei1186 – 11861PhosphothreonineBy similarity
Modified residuei1278 – 12781PhosphoserineBy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ8K4L3.
PaxDbiQ8K4L3.
PRIDEiQ8K4L3.

PTM databases

PhosphoSiteiQ8K4L3.

Expressioni

Gene expression databases

BgeeiQ8K4L3.
CleanExiMM_SVIL.
ExpressionAtlasiQ8K4L3. baseline and differential.
GenevestigatoriQ8K4L3.

Interactioni

Subunit structurei

Associates with F-actin. Interacts with NEB. Interacts with MYH9. Interacts with MYLK. Isoform 2 interacts with TRIP6. Isoform 2 interacts with DYNLT1. Interacts with KIF14; at midbody during cytokinesis (By similarity).By similarity

Protein-protein interaction databases

BioGridi230358. 1 interaction.
IntActiQ8K4L3. 1 interaction.
MINTiMINT-4136191.

Structurei

3D structure databases

ProteinModelPortaliQ8K4L3.
SMRiQ8K4L3. Positions 1398-1640, 1712-2079, 2104-2170.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati1397 – 1496100Gelsolin-like 1Add
BLAST
Repeati1516 – 1638123Gelsolin-like 2Add
BLAST
Repeati1708 – 1818111Gelsolin-like 3Add
BLAST
Repeati1837 – 1938102Gelsolin-like 4Add
BLAST
Repeati1971 – 2078108Gelsolin-like 5Add
BLAST
Domaini2107 – 217064HPPROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 167167Interaction with MYLKBy similarityAdd
BLAST
Regioni1375 – 1643269Interaction with NEBBy similarityAdd
BLAST

Domaini

As opposed to other villin-type headpiece domains, supervillin HP (SVHP) doesn't bind F-actin due to the absence of a conformationally flexible region (V-loop).By similarity

Sequence similaritiesi

Belongs to the villin/gelsolin family.Curated
Contains 5 gelsolin-like repeats.Curated
Contains 1 HP (headpiece) domain.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiNOG324767.
GeneTreeiENSGT00760000119111.
HOGENOMiHOG000006939.
HOVERGENiHBG052980.
InParanoidiQ8K4L3.
KOiK10369.
OMAiRSHTQPI.
OrthoDBiEOG7WX07J.
PhylomeDBiQ8K4L3.
TreeFamiTF316081.

Family and domain databases

Gene3Di1.10.950.10. 1 hit.
3.40.20.10. 5 hits.
InterProiIPR029006. ADF-H/Gelsolin-like_dom.
IPR007123. Gelsolin-like_dom.
IPR015628. SV/p205.
IPR007122. Villin/Gelsolin.
IPR003128. Villin_headpiece.
[Graphical view]
PANTHERiPTHR11977. PTHR11977. 1 hit.
PTHR11977:SF28. PTHR11977:SF28. 1 hit.
PfamiPF00626. Gelsolin. 1 hit.
PF02209. VHP. 1 hit.
[Graphical view]
PRINTSiPR00597. GELSOLIN.
SMARTiSM00262. GEL. 5 hits.
SM00153. VHP. 1 hit.
[Graphical view]
SUPFAMiSSF47050. SSF47050. 1 hit.
PROSITEiPS51089. HP. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: Q8K4L3-1) [UniParc]FASTAAdd to Basket

Also known as: Archvillin

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MKRKERIARR LEGIENDSQP ILLQSCTGLV THRLLEEDTP RYMRATDPAS
60 70 80 90 100
PHIGRSKEEE DTPGSSLEKQ TPSKYCIETS GIHSSGSMDT HSLESKAERI
110 120 130 140 150
ARYKAERRRQ LAEKYGLTLD PEADSEYLSR YAKSRKDPDV TERRGKSDKQ
160 170 180 190 200
EEQSKDANSR HSRTESGPRT SLVASQDCTP LGSNMSDQEQ LLNVENQRRV
210 220 230 240 250
QDPPLGEDGS SAFFSERSIS FPEVPRSPKQ IPSSPLQQPA SPNHPGDSPL
260 270 280 290 300
PTEARASTGK PTHEWFLQRD SEGDTPSLIN WPSRVKVREK LVKEESARSS
310 320 330 340 350
PELTSESLTQ RRQQPAPAHF LPIQSESSTF DRVTSKAVSS LQPSQSGVLP
360 370 380 390 400
TDPVHAIKLV TMDTPESTSE FSWVGSATPK VIKSTTLKIL EGGSRDAPVL
410 420 430 440 450
HICESKAEDW LSPEPLERSP KSLLTSEDDR LVRGHKDPSG NKDLDKAIIC
460 470 480 490 500
SIDVESERER QVQHLPTQRT GRSEMLLYVQ SGPVSQDATL TSHTKEASPK
510 520 530 540 550
KRKVLARSLS DYTGPPQLQV PRHKDEAPSQ ELELQSSRAE GPGAEASVLD
560 570 580 590 600
TRVSVAQLRN IFMESTRASK KPELQSRVER SAEGIGLPME RERGSRKPRR
610 620 630 640 650
YLSPGESRKT SERFRTQPIT SAERKESDRY PSGSEIPVVE DEEKVDERAK
660 670 680 690 700
LSVAAKRLLF REMEKSFDEH TVPKRHSRNA AVEQRLRRLQ DRSHTQPITT
710 720 730 740 750
EEVVIAATEP IPASCSGVTH PVTARLPSPT VARSSVQPAR LQASAHQKAL
760 770 780 790 800
ARDQANEGRE SAEPGEPDSS TLSLAEKLAL FNKLSQPVSK AISTRNRIDV
810 820 830 840 850
RQRRMNARYQ TQPVTLGEVE QVQSGKLISF SPTVNTSVSI MASAVAPTYA
860 870 880 890 900
GDLRKLSVDN NTSATDYKSP PAENSDSPVR SILKPQAWRP LVEHSGSKGM
910 920 930 940 950
PGESGKTESK NALTVAAEDS GVQTRGAFEE EEEPSYPILG RVREGDGQKE
960 970 980 990 1000
PKHVVLRRGS LELGNPSAAH LGDELKEVST AKSSLQENLD LKDKQASEEN
1010 1020 1030 1040 1050
TDVETVMRKF SLKEFGETTS EQTEVAARKA SVQMATPGAW KQQESSEQLA
1060 1070 1080 1090 1100
EKLFKNPCAM FASGEVKVPV GDSFLDSPSK TMSIKERLAL LKKSGEEDWK
1110 1120 1130 1140 1150
NRLIRKQEYG KATGGLHTQE VEQSLKKKRV TESRESQMTI EERKHLITVR
1160 1170 1180 1190 1200
EEAWKTKGRG AANDSTQFTV AGRMVKKGLA SPTSITPISS PLCSKSRGTT
1210 1220 1230 1240 1250
PVSKPLEDIE ARPDMQLESD LKLDRLETFL RRLNNKVAGI QETVLTVTGK
1260 1270 1280 1290 1300
SVKEVMKLDD DETFAKFYRS VDHSIPRSPV ELEEDFDVIF DPYAPKLTSS
1310 1320 1330 1340 1350
VAEHKRQVRP KRRVQASKNP LKLLAARDDL LQEYTEQRLN VAFMESKRMK
1360 1370 1380 1390 1400
VEKMSSNSNF SEVTLAGLAS RENFSNINLR SVNLMEQNSN NSAMPYKKLM
1410 1420 1430 1440 1450
LLQIKGRRHV QTRLVEPRAS SLNSGDCFLL LSPQYCFLWV GEFSNVIEKA
1460 1470 1480 1490 1500
KASELATLIQ TKRELGCRAT YIQTIEEGIN THTHAAKDFW KLLGGQTSYQ
1510 1520 1530 1540 1550
SAGDPKEDEL YETAIIETNC VYRLTDDKLV PDDDYWGKIP KCSLLQSKEV
1560 1570 1580 1590 1600
LVFDFGSEVY VWHGKEVTLA QRKIAFQLAK HLWNGTFDYE NCDINPLDPG
1610 1620 1630 1640 1650
ECNPLIPRKG QGRPDWAIFG RVTEHNETIL FKEKFLDWTE LKRPTEKNSG
1660 1670 1680 1690 1700
EVVQQKDDPR ADVKPYDVTR MVATPQITAG TILDGVNVGR GYGLVEGDDR
1710 1720 1730 1740 1750
RQFEIATVSV DVWHILEFDY SRLPRQSIGQ FHEGDAYVVK WKYMASTAVG
1760 1770 1780 1790 1800
SRQKGEHLVR VAGKEKCVYF FWQGRHSTVS EKGTSALMTV ELDEERGAQV
1810 1820 1830 1840 1850
QVLQGKEPPC FLQCFQGGMV VHSGRREEEE ENVQSEWRLY CVRGEVPMEG
1860 1870 1880 1890 1900
NLLEVACHCS SLRSRTSMVV LNINKALIYL WHGCKAQGHT KEVGRTAANK
1910 1920 1930 1940 1950
IKEECPLEAG LHSSSNVTIH ECDEGSEPLG FWDALGRRDR KAYDCMLQDP
1960 1970 1980 1990 2000
GSFNFAPRLF ILSSSSGDFS ATEFVYPAQA PSAVSSMPFL QEDLYSAPQP
2010 2020 2030 2040 2050
ALFLVDNHHE VYLWQGWWPT ENKITGSARI RWASDRKSAM ETVLQYCRGK
2060 2070 2080 2090 2100
NLKRPPPKSY LIHAGLEPLT FTNMFPSWEH REDIAEITEM DTEVSNQITL
2110 2120 2130 2140 2150
VEDVLAKLCK TIYPLADLLA RPLPEGVDPL KLEIYLTDED FEFALDMSRD
2160 2170
EFNALPTWKQ VNLKKSKGLF
Length:2,170
Mass (Da):243,162
Last modified:October 1, 2002 - v1
Checksum:iAEAB710184FB71C6
GO
Isoform 2 (identifier: Q8K4L3-2)

Also known as: Supervillin

Sequence is not available
Length:
Mass (Da):

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF317422 mRNA. Translation: AAM89518.1.
CCDSiCCDS37720.1. [Q8K4L3-1]
RefSeqiNP_694793.1. NM_153153.3. [Q8K4L3-1]
UniGeneiMm.136791.

Genome annotation databases

EnsembliENSMUST00000025079; ENSMUSP00000025079; ENSMUSG00000024236. [Q8K4L3-1]
ENSMUST00000126977; ENSMUSP00000115078; ENSMUSG00000024236. [Q8K4L3-1]
ENSMUST00000140448; ENSMUSP00000119803; ENSMUSG00000024236. [Q8K4L3-1]
GeneIDi225115.
KEGGimmu:225115.
UCSCiuc008dyr.2. mouse. [Q8K4L3-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF317422 mRNA. Translation: AAM89518.1.
CCDSiCCDS37720.1. [Q8K4L3-1]
RefSeqiNP_694793.1. NM_153153.3. [Q8K4L3-1]
UniGeneiMm.136791.

3D structure databases

ProteinModelPortaliQ8K4L3.
SMRiQ8K4L3. Positions 1398-1640, 1712-2079, 2104-2170.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi230358. 1 interaction.
IntActiQ8K4L3. 1 interaction.
MINTiMINT-4136191.

PTM databases

PhosphoSiteiQ8K4L3.

Proteomic databases

MaxQBiQ8K4L3.
PaxDbiQ8K4L3.
PRIDEiQ8K4L3.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000025079; ENSMUSP00000025079; ENSMUSG00000024236. [Q8K4L3-1]
ENSMUST00000126977; ENSMUSP00000115078; ENSMUSG00000024236. [Q8K4L3-1]
ENSMUST00000140448; ENSMUSP00000119803; ENSMUSG00000024236. [Q8K4L3-1]
GeneIDi225115.
KEGGimmu:225115.
UCSCiuc008dyr.2. mouse. [Q8K4L3-1]

Organism-specific databases

CTDi6840.
MGIiMGI:2147319. Svil.

Phylogenomic databases

eggNOGiNOG324767.
GeneTreeiENSGT00760000119111.
HOGENOMiHOG000006939.
HOVERGENiHBG052980.
InParanoidiQ8K4L3.
KOiK10369.
OMAiRSHTQPI.
OrthoDBiEOG7WX07J.
PhylomeDBiQ8K4L3.
TreeFamiTF316081.

Miscellaneous databases

ChiTaRSiSvil. mouse.
NextBioi377534.
PROiQ8K4L3.
SOURCEiSearch...

Gene expression databases

BgeeiQ8K4L3.
CleanExiMM_SVIL.
ExpressionAtlasiQ8K4L3. baseline and differential.
GenevestigatoriQ8K4L3.

Family and domain databases

Gene3Di1.10.950.10. 1 hit.
3.40.20.10. 5 hits.
InterProiIPR029006. ADF-H/Gelsolin-like_dom.
IPR007123. Gelsolin-like_dom.
IPR015628. SV/p205.
IPR007122. Villin/Gelsolin.
IPR003128. Villin_headpiece.
[Graphical view]
PANTHERiPTHR11977. PTHR11977. 1 hit.
PTHR11977:SF28. PTHR11977:SF28. 1 hit.
PfamiPF00626. Gelsolin. 1 hit.
PF02209. VHP. 1 hit.
[Graphical view]
PRINTSiPR00597. GELSOLIN.
SMARTiSM00262. GEL. 5 hits.
SM00153. VHP. 1 hit.
[Graphical view]
SUPFAMiSSF47050. SSF47050. 1 hit.
PROSITEiPS51089. HP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Archvillin, a muscle-specific isoform of supervillin, is an early expressed component of the costameric membrane skeleton."
    Oh S.W., Pope R.K., Smith K.P., Crowley J.L., Nebl T., Lawrence J.B., Luna E.J.
    J. Cell Sci. 116:2261-2275(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Skeletal muscle.
  2. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-857 AND SER-960, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Liver.
  3. "The phagosomal proteome in interferon-gamma-activated macrophages."
    Trost M., English L., Lemieux S., Courcelles M., Desjardins M., Thibault P.
    Immunity 30:143-154(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-960, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  4. "Large scale localization of protein phosphorylation by use of electron capture dissociation mass spectrometry."
    Sweet S.M., Bailey C.M., Cunningham D.L., Heath J.K., Cooper H.J.
    Mol. Cell. Proteomics 8:904-912(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-227, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Embryonic fibroblast.

Entry informationi

Entry nameiSVIL_MOUSE
AccessioniPrimary (citable) accession number: Q8K4L3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 4, 2005
Last sequence update: October 1, 2002
Last modified: February 4, 2015
This is version 107 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.