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Q8K4L3

- SVIL_MOUSE

UniProt

Q8K4L3 - SVIL_MOUSE

Protein

Supervillin

Gene

Svil

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 103 (01 Oct 2014)
      Sequence version 1 (01 Oct 2002)
      Previous versions | rss
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    Functioni

    Forms a high-affinity link between the actin cytoskeleton and the membrane. Isoform 1 (archvillin) is among the first costameric proteins to assemble during myogenesis and it contributes to myogenic membrane structure and differentiation. Appears to be involved in myosin II assembly. May modulate myosin II regulation through MLCK during cell spreading, an initial step in cell migration. May play a role in invadopodial function. Isoform 2 may be involved in modulation of focal adhesions. Supervillin-mediated down-regulation of focal adhesions involves binding to TRIP6 By similarity.By similarity

    GO - Biological processi

    1. cytoskeleton organization Source: InterPro
    2. skeletal muscle tissue development Source: Ensembl

    Keywords - Ligandi

    Actin-binding, Calcium

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Supervillin
    Alternative name(s):
    Archvillin
    p205/p250
    Gene namesi
    Name:Svil
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 18

    Organism-specific databases

    MGIiMGI:2147319. Svil.

    Subcellular locationi

    Cell membrane; Peripheral membrane protein; Cytoplasmic side. Cytoplasmcytoskeleton. Cell projectioninvadopodium By similarity. Cell projectionpodosome By similarity
    Note: Tightly associated with both actin filaments and plasma membranes.

    GO - Cellular componenti

    1. cell junction Source: UniProtKB-KW
    2. costamere Source: Ensembl
    3. invadopodium Source: UniProtKB-SubCell
    4. nucleus Source: Ensembl
    5. plasma membrane Source: UniProtKB-SubCell
    6. podosome Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cell junction, Cell membrane, Cell projection, Cytoplasm, Cytoskeleton, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 21702170SupervillinPRO_0000218741Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei50 – 501PhosphoserineBy similarity
    Modified residuei227 – 2271Phosphoserine1 Publication
    Modified residuei666 – 6661PhosphoserineBy similarity
    Modified residuei811 – 8111PhosphothreonineBy similarity
    Modified residuei857 – 8571Phosphoserine1 Publication
    Modified residuei877 – 8771PhosphoserineBy similarity
    Modified residuei881 – 8811PhosphoserineBy similarity
    Modified residuei960 – 9601Phosphoserine2 Publications
    Modified residuei1077 – 10771PhosphoserineBy similarity
    Modified residuei1181 – 11811PhosphoserineBy similarity
    Modified residuei1186 – 11861PhosphothreonineBy similarity
    Modified residuei1278 – 12781PhosphoserineBy similarity

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiQ8K4L3.
    PaxDbiQ8K4L3.
    PRIDEiQ8K4L3.

    PTM databases

    PhosphoSiteiQ8K4L3.

    Expressioni

    Gene expression databases

    ArrayExpressiQ8K4L3.
    BgeeiQ8K4L3.
    CleanExiMM_SVIL.
    GenevestigatoriQ8K4L3.

    Interactioni

    Subunit structurei

    Associates with F-actin. Interacts with NEB. Interacts with MYH9. Interacts with MYLK. Isoform 2 interacts with TRIP6. Isoform 2 interacts with DYNLT1 By similarity.By similarity

    Protein-protein interaction databases

    BioGridi230358. 1 interaction.
    IntActiQ8K4L3. 1 interaction.
    MINTiMINT-4136191.

    Structurei

    3D structure databases

    ProteinModelPortaliQ8K4L3.
    SMRiQ8K4L3. Positions 1398-1640, 1712-2079, 2104-2170.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Repeati1397 – 1496100Gelsolin-like 1Add
    BLAST
    Repeati1516 – 1638123Gelsolin-like 2Add
    BLAST
    Repeati1708 – 1818111Gelsolin-like 3Add
    BLAST
    Repeati1837 – 1938102Gelsolin-like 4Add
    BLAST
    Repeati1971 – 2078108Gelsolin-like 5Add
    BLAST
    Domaini2107 – 217064HPPROSITE-ProRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni1 – 167167Interaction with MYLKBy similarityAdd
    BLAST
    Regioni1375 – 1643269Interaction with NEBBy similarityAdd
    BLAST

    Domaini

    As opposed to other villin-type headpiece domains, supervillin HP (SVHP) doesn't bind F-actin due to the absence of a conformationally flexible region (V-loop).By similarity

    Sequence similaritiesi

    Belongs to the villin/gelsolin family.Curated
    Contains 5 gelsolin-like repeats.Curated
    Contains 1 HP (headpiece) domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Repeat

    Phylogenomic databases

    eggNOGiNOG324767.
    GeneTreeiENSGT00660000095502.
    HOGENOMiHOG000006939.
    HOVERGENiHBG052980.
    InParanoidiQ8K4L3.
    KOiK10369.
    OMAiRSHTQPI.
    OrthoDBiEOG7WX07J.
    PhylomeDBiQ8K4L3.
    TreeFamiTF316081.

    Family and domain databases

    Gene3Di1.10.950.10. 1 hit.
    3.40.20.10. 5 hits.
    InterProiIPR029006. ADF-H/Gelsolin-like_dom.
    IPR007123. Gelsolin-like_dom.
    IPR015628. Supervillin.
    IPR007122. Villin/Gelsolin.
    IPR003128. Villin_headpiece.
    [Graphical view]
    PANTHERiPTHR11977. PTHR11977. 1 hit.
    PTHR11977:SF28. PTHR11977:SF28. 1 hit.
    PfamiPF00626. Gelsolin. 1 hit.
    PF02209. VHP. 1 hit.
    [Graphical view]
    PRINTSiPR00597. GELSOLIN.
    SMARTiSM00262. GEL. 5 hits.
    SM00153. VHP. 1 hit.
    [Graphical view]
    SUPFAMiSSF47050. SSF47050. 1 hit.
    PROSITEiPS51089. HP. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q8K4L3-1) [UniParc]FASTAAdd to Basket

    Also known as: Archvillin

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MKRKERIARR LEGIENDSQP ILLQSCTGLV THRLLEEDTP RYMRATDPAS     50
    PHIGRSKEEE DTPGSSLEKQ TPSKYCIETS GIHSSGSMDT HSLESKAERI 100
    ARYKAERRRQ LAEKYGLTLD PEADSEYLSR YAKSRKDPDV TERRGKSDKQ 150
    EEQSKDANSR HSRTESGPRT SLVASQDCTP LGSNMSDQEQ LLNVENQRRV 200
    QDPPLGEDGS SAFFSERSIS FPEVPRSPKQ IPSSPLQQPA SPNHPGDSPL 250
    PTEARASTGK PTHEWFLQRD SEGDTPSLIN WPSRVKVREK LVKEESARSS 300
    PELTSESLTQ RRQQPAPAHF LPIQSESSTF DRVTSKAVSS LQPSQSGVLP 350
    TDPVHAIKLV TMDTPESTSE FSWVGSATPK VIKSTTLKIL EGGSRDAPVL 400
    HICESKAEDW LSPEPLERSP KSLLTSEDDR LVRGHKDPSG NKDLDKAIIC 450
    SIDVESERER QVQHLPTQRT GRSEMLLYVQ SGPVSQDATL TSHTKEASPK 500
    KRKVLARSLS DYTGPPQLQV PRHKDEAPSQ ELELQSSRAE GPGAEASVLD 550
    TRVSVAQLRN IFMESTRASK KPELQSRVER SAEGIGLPME RERGSRKPRR 600
    YLSPGESRKT SERFRTQPIT SAERKESDRY PSGSEIPVVE DEEKVDERAK 650
    LSVAAKRLLF REMEKSFDEH TVPKRHSRNA AVEQRLRRLQ DRSHTQPITT 700
    EEVVIAATEP IPASCSGVTH PVTARLPSPT VARSSVQPAR LQASAHQKAL 750
    ARDQANEGRE SAEPGEPDSS TLSLAEKLAL FNKLSQPVSK AISTRNRIDV 800
    RQRRMNARYQ TQPVTLGEVE QVQSGKLISF SPTVNTSVSI MASAVAPTYA 850
    GDLRKLSVDN NTSATDYKSP PAENSDSPVR SILKPQAWRP LVEHSGSKGM 900
    PGESGKTESK NALTVAAEDS GVQTRGAFEE EEEPSYPILG RVREGDGQKE 950
    PKHVVLRRGS LELGNPSAAH LGDELKEVST AKSSLQENLD LKDKQASEEN 1000
    TDVETVMRKF SLKEFGETTS EQTEVAARKA SVQMATPGAW KQQESSEQLA 1050
    EKLFKNPCAM FASGEVKVPV GDSFLDSPSK TMSIKERLAL LKKSGEEDWK 1100
    NRLIRKQEYG KATGGLHTQE VEQSLKKKRV TESRESQMTI EERKHLITVR 1150
    EEAWKTKGRG AANDSTQFTV AGRMVKKGLA SPTSITPISS PLCSKSRGTT 1200
    PVSKPLEDIE ARPDMQLESD LKLDRLETFL RRLNNKVAGI QETVLTVTGK 1250
    SVKEVMKLDD DETFAKFYRS VDHSIPRSPV ELEEDFDVIF DPYAPKLTSS 1300
    VAEHKRQVRP KRRVQASKNP LKLLAARDDL LQEYTEQRLN VAFMESKRMK 1350
    VEKMSSNSNF SEVTLAGLAS RENFSNINLR SVNLMEQNSN NSAMPYKKLM 1400
    LLQIKGRRHV QTRLVEPRAS SLNSGDCFLL LSPQYCFLWV GEFSNVIEKA 1450
    KASELATLIQ TKRELGCRAT YIQTIEEGIN THTHAAKDFW KLLGGQTSYQ 1500
    SAGDPKEDEL YETAIIETNC VYRLTDDKLV PDDDYWGKIP KCSLLQSKEV 1550
    LVFDFGSEVY VWHGKEVTLA QRKIAFQLAK HLWNGTFDYE NCDINPLDPG 1600
    ECNPLIPRKG QGRPDWAIFG RVTEHNETIL FKEKFLDWTE LKRPTEKNSG 1650
    EVVQQKDDPR ADVKPYDVTR MVATPQITAG TILDGVNVGR GYGLVEGDDR 1700
    RQFEIATVSV DVWHILEFDY SRLPRQSIGQ FHEGDAYVVK WKYMASTAVG 1750
    SRQKGEHLVR VAGKEKCVYF FWQGRHSTVS EKGTSALMTV ELDEERGAQV 1800
    QVLQGKEPPC FLQCFQGGMV VHSGRREEEE ENVQSEWRLY CVRGEVPMEG 1850
    NLLEVACHCS SLRSRTSMVV LNINKALIYL WHGCKAQGHT KEVGRTAANK 1900
    IKEECPLEAG LHSSSNVTIH ECDEGSEPLG FWDALGRRDR KAYDCMLQDP 1950
    GSFNFAPRLF ILSSSSGDFS ATEFVYPAQA PSAVSSMPFL QEDLYSAPQP 2000
    ALFLVDNHHE VYLWQGWWPT ENKITGSARI RWASDRKSAM ETVLQYCRGK 2050
    NLKRPPPKSY LIHAGLEPLT FTNMFPSWEH REDIAEITEM DTEVSNQITL 2100
    VEDVLAKLCK TIYPLADLLA RPLPEGVDPL KLEIYLTDED FEFALDMSRD 2150
    EFNALPTWKQ VNLKKSKGLF 2170
    Length:2,170
    Mass (Da):243,162
    Last modified:October 1, 2002 - v1
    Checksum:iAEAB710184FB71C6
    GO
    Isoform 2 (identifier: Q8K4L3-2)

    Also known as: Supervillin

    Sequence is not available
    Length:
    Mass (Da):

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF317422 mRNA. Translation: AAM89518.1.
    CCDSiCCDS37720.1. [Q8K4L3-1]
    RefSeqiNP_694793.1. NM_153153.3. [Q8K4L3-1]
    UniGeneiMm.136791.

    Genome annotation databases

    EnsembliENSMUST00000025079; ENSMUSP00000025079; ENSMUSG00000024236. [Q8K4L3-1]
    ENSMUST00000126977; ENSMUSP00000115078; ENSMUSG00000024236. [Q8K4L3-1]
    ENSMUST00000140448; ENSMUSP00000119803; ENSMUSG00000024236. [Q8K4L3-1]
    GeneIDi225115.
    KEGGimmu:225115.
    UCSCiuc008dyr.2. mouse. [Q8K4L3-1]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF317422 mRNA. Translation: AAM89518.1 .
    CCDSi CCDS37720.1. [Q8K4L3-1 ]
    RefSeqi NP_694793.1. NM_153153.3. [Q8K4L3-1 ]
    UniGenei Mm.136791.

    3D structure databases

    ProteinModelPortali Q8K4L3.
    SMRi Q8K4L3. Positions 1398-1640, 1712-2079, 2104-2170.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 230358. 1 interaction.
    IntActi Q8K4L3. 1 interaction.
    MINTi MINT-4136191.

    PTM databases

    PhosphoSitei Q8K4L3.

    Proteomic databases

    MaxQBi Q8K4L3.
    PaxDbi Q8K4L3.
    PRIDEi Q8K4L3.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000025079 ; ENSMUSP00000025079 ; ENSMUSG00000024236 . [Q8K4L3-1 ]
    ENSMUST00000126977 ; ENSMUSP00000115078 ; ENSMUSG00000024236 . [Q8K4L3-1 ]
    ENSMUST00000140448 ; ENSMUSP00000119803 ; ENSMUSG00000024236 . [Q8K4L3-1 ]
    GeneIDi 225115.
    KEGGi mmu:225115.
    UCSCi uc008dyr.2. mouse. [Q8K4L3-1 ]

    Organism-specific databases

    CTDi 6840.
    MGIi MGI:2147319. Svil.

    Phylogenomic databases

    eggNOGi NOG324767.
    GeneTreei ENSGT00660000095502.
    HOGENOMi HOG000006939.
    HOVERGENi HBG052980.
    InParanoidi Q8K4L3.
    KOi K10369.
    OMAi RSHTQPI.
    OrthoDBi EOG7WX07J.
    PhylomeDBi Q8K4L3.
    TreeFami TF316081.

    Miscellaneous databases

    ChiTaRSi SVIL. mouse.
    NextBioi 377534.
    PROi Q8K4L3.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q8K4L3.
    Bgeei Q8K4L3.
    CleanExi MM_SVIL.
    Genevestigatori Q8K4L3.

    Family and domain databases

    Gene3Di 1.10.950.10. 1 hit.
    3.40.20.10. 5 hits.
    InterProi IPR029006. ADF-H/Gelsolin-like_dom.
    IPR007123. Gelsolin-like_dom.
    IPR015628. Supervillin.
    IPR007122. Villin/Gelsolin.
    IPR003128. Villin_headpiece.
    [Graphical view ]
    PANTHERi PTHR11977. PTHR11977. 1 hit.
    PTHR11977:SF28. PTHR11977:SF28. 1 hit.
    Pfami PF00626. Gelsolin. 1 hit.
    PF02209. VHP. 1 hit.
    [Graphical view ]
    PRINTSi PR00597. GELSOLIN.
    SMARTi SM00262. GEL. 5 hits.
    SM00153. VHP. 1 hit.
    [Graphical view ]
    SUPFAMi SSF47050. SSF47050. 1 hit.
    PROSITEi PS51089. HP. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Archvillin, a muscle-specific isoform of supervillin, is an early expressed component of the costameric membrane skeleton."
      Oh S.W., Pope R.K., Smith K.P., Crowley J.L., Nebl T., Lawrence J.B., Luna E.J.
      J. Cell Sci. 116:2261-2275(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Skeletal muscle.
    2. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-857 AND SER-960, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Liver.
    3. "The phagosomal proteome in interferon-gamma-activated macrophages."
      Trost M., English L., Lemieux S., Courcelles M., Desjardins M., Thibault P.
      Immunity 30:143-154(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-960, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    4. "Large scale localization of protein phosphorylation by use of electron capture dissociation mass spectrometry."
      Sweet S.M., Bailey C.M., Cunningham D.L., Heath J.K., Cooper H.J.
      Mol. Cell. Proteomics 8:904-912(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-227, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Embryonic fibroblast.

    Entry informationi

    Entry nameiSVIL_MOUSE
    AccessioniPrimary (citable) accession number: Q8K4L3
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 4, 2005
    Last sequence update: October 1, 2002
    Last modified: October 1, 2014
    This is version 103 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3