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Protein

Actin-binding Rho-activating protein

Gene

Abra

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Acts as an activator of serum response factor (SRF)-dependent transcription possibly by inducing nuclear translocation of MKL1 or MKL2 and through a mechanism requiring Rho-actin signaling.By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Activator

Keywords - Biological processi

Protein transport, Transcription, Transcription regulation, Translocation, Transport

Keywords - Ligandi

Actin-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Actin-binding Rho-activating protein
Alternative name(s):
MS1
Striated muscle activator of Rho-dependent signaling
Short name:
STARS
Gene namesi
Name:AbraImported
Synonyms:Ms1Imported
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 7

Organism-specific databases

RGDi708493. Abra.

Subcellular locationi

GO - Cellular componenti

  • actin cytoskeleton Source: HGNC
  • plasma membrane Source: Ensembl
  • sarcomere Source: HGNC
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 375375Actin-binding Rho-activating proteinPRO_0000247741Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei150 – 1501PhosphoserineCombined sources
Modified residuei182 – 1821PhosphoserineCombined sources

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ8K4K7.
PRIDEiQ8K4K7.

PTM databases

iPTMnetiQ8K4K7.
PhosphoSiteiQ8K4K7.

Expressioni

Tissue specificityi

Predominantly expressed in heart and skeletal muscle, and expressed at lower levels in adrenal gland, brain, kidney, liver, and testis.1 Publication

Developmental stagei

Expressed in the left ventricle of embryonic heart and is postnatally up-regulated through to adulthood.1 Publication

Inductioni

Up-regulated within 1 hour in the left ventricle following the application of pressure overload by aortic banding.1 Publication

Gene expression databases

GenevisibleiQ8K4K7. RN.

Interactioni

Subunit structurei

Binds F-actin and ABLIM1, ABLIM2 AND ABLIM3. Interaction with ABLIM2 AND ABLIM3 enhances activity (By similarity).By similarity

GO - Molecular functioni

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000010539.

Structurei

Secondary structure

1
375
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi297 – 2993Combined sources
Helixi305 – 31410Combined sources
Helixi316 – 3194Combined sources
Beta strandi324 – 3263Combined sources
Helixi327 – 3348Combined sources
Turni335 – 3384Combined sources
Helixi341 – 3499Combined sources
Turni363 – 3653Combined sources
Beta strandi370 – 3734Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2KRHNMR-A295-375[»]
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ8K4K7.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni193 – 293101Actin-binding 1Add
BLAST
Regioni234 – 27946Interaction with actinBy similarityAdd
BLAST
Regioni294 – 37582Actin-binding 2Add
BLAST
Regioni346 – 37530Interaction with actinBy similarityAdd
BLAST

Domaini

The actin-binding domain 1 (ABD1) is intrinsically disordered, and binds to F-actin with higher affinity than ABD2.

Phylogenomic databases

eggNOGiKOG3376. Eukaryota.
ENOG410XR7V. LUCA.
GeneTreeiENSGT00390000015984.
HOGENOMiHOG000033774.
HOVERGENiHBG060846.
InParanoidiQ8K4K7.
OMAiQQWANEN.
OrthoDBiEOG7PGDTD.
PhylomeDBiQ8K4K7.
TreeFamiTF328879.

Family and domain databases

InterProiIPR026111. Abra.
IPR027817. Costars_dom.
[Graphical view]
PANTHERiPTHR22739:SF7. PTHR22739:SF7. 1 hit.
PfamiPF14705. Costars. 1 hit.
[Graphical view]
SMARTiSM01283. Costars. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8K4K7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAPGETEREA GPAKSALQKV RRATLVINLA RGWQQWANEN STRQAQEPAG
60 70 80 90 100
WLPGATQDLP HTPKEPGPRQ HAPKPPSPKP DGDREGRGSE EATEVSHIKR
110 120 130 140 150
KEVTRTVVSK AYERGGDVNY LSHRYEHDGG VSEAVQPDND IDRILLSHDS
160 170 180 190 200
PTRRRKCTNL VSELTKGWKV MEQEEPKWKS DSIDTEDSGY GGDMEERPEQ
210 220 230 240 250
DVAQVAAARI KRPLHSQANR YSETLNCKAH RKYSQVDNLK GRWQQWADEH
260 270 280 290 300
IQSQKLNPFS DEFDYDLAMS TRLHKGDEGY GRPKEGSKTA ERAKRAEEHI
310 320 330 340 350
YREIMELCFV IRTMARHRRD GKIQVTFGEL FDRYVRISDK VVGILMRARK
360 370
HGLVHFEGEM LWQGKDDHVV ITLLE
Length:375
Mass (Da):42,901
Last modified:March 24, 2009 - v2
Checksum:i3DFEAEB9970B466F
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti7 – 71E → V in AAM94370 (PubMed:12067735).Curated
Sequence conflicti163 – 1631E → K in AAM94370 (PubMed:12067735).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF336113 mRNA. Translation: AAM94370.1.
BC158575 mRNA. Translation: AAI58576.1.
RefSeqiNP_787038.1. NM_175844.3.
UniGeneiRn.21198.

Genome annotation databases

EnsembliENSRNOT00000010539; ENSRNOP00000010539; ENSRNOG00000007999.
GeneIDi286965.
KEGGirno:286965.
UCSCiRGD:708493. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF336113 mRNA. Translation: AAM94370.1.
BC158575 mRNA. Translation: AAI58576.1.
RefSeqiNP_787038.1. NM_175844.3.
UniGeneiRn.21198.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2KRHNMR-A295-375[»]
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000010539.

PTM databases

iPTMnetiQ8K4K7.
PhosphoSiteiQ8K4K7.

Proteomic databases

PaxDbiQ8K4K7.
PRIDEiQ8K4K7.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000010539; ENSRNOP00000010539; ENSRNOG00000007999.
GeneIDi286965.
KEGGirno:286965.
UCSCiRGD:708493. rat.

Organism-specific databases

CTDi137735.
RGDi708493. Abra.

Phylogenomic databases

eggNOGiKOG3376. Eukaryota.
ENOG410XR7V. LUCA.
GeneTreeiENSGT00390000015984.
HOGENOMiHOG000033774.
HOVERGENiHBG060846.
InParanoidiQ8K4K7.
OMAiQQWANEN.
OrthoDBiEOG7PGDTD.
PhylomeDBiQ8K4K7.
TreeFamiTF328879.

Miscellaneous databases

EvolutionaryTraceiQ8K4K7.
PROiQ8K4K7.

Gene expression databases

GenevisibleiQ8K4K7. RN.

Family and domain databases

InterProiIPR026111. Abra.
IPR027817. Costars_dom.
[Graphical view]
PANTHERiPTHR22739:SF7. PTHR22739:SF7. 1 hit.
PfamiPF14705. Costars. 1 hit.
[Graphical view]
SMARTiSM01283. Costars. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "ms1, a novel stress-responsive, muscle-specific gene that is up-regulated in the early stages of pressure overload-induced left ventricular hypertrophy."
    Mahadeva H., Brooks G., Lodwick D., Chong N.W., Samani N.J.
    FEBS Lett. 521:100-104(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, INDUCTION.
    Strain: Wistar KyotoImported.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Heart.
  3. "STARS, a striated muscle activator of Rho signaling and serum response factor-dependent transcription."
    Arai A., Spencer J.A., Olson E.N.
    J. Biol. Chem. 277:24453-24459(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION.
  4. "Quantitative maps of protein phosphorylation sites across 14 different rat organs and tissues."
    Lundby A., Secher A., Lage K., Nordsborg N.B., Dmytriyev A., Lundby C., Olsen J.V.
    Nat. Commun. 3:876-876(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-150 AND SER-182, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  5. "A structural and functional dissection of the cardiac stress response factor MS1."
    Fogl C., Puckey L., Hinssen U., Zaleska M., El-Mezgueldi M., Croasdale R., Bowman A., Matsukawa A., Samani N.J., Savva R., Pfuhl M.
    Proteins 80:398-409(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: STRUCTURE BY NMR OF 295-375, SUBUNIT, ACTIN-BINDING DOMAINS.

Entry informationi

Entry nameiABRA_RAT
AccessioniPrimary (citable) accession number: Q8K4K7
Secondary accession number(s): B0BMV2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 25, 2006
Last sequence update: March 24, 2009
Last modified: June 8, 2016
This is version 78 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.