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Protein

Pantothenate kinase 1

Gene

Pank1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Plays a role in the physiological regulation of the intracellular CoA concentration.

Catalytic activityi

ATP + (R)-pantothenate = ADP + (R)-4'-phosphopantothenate.

Enzyme regulationi

Regulated by feedback inhibition by CoA and its thioesters. Strongly inhibited by acetyl-CoA and by manyl-CoA and also inhibited by high concentration of non-esterified CoA (CoASH). Isoform 1 is inhibited by high concentration of non-esterified CoA (CoASH) and strongly inhibited by acetyl-CoA and by malonyl-CoA. Isoform 2 is stimulated by CoA and weakly inhibited by acetyl-CoA and malonyl-CoA.

Pathwayi: coenzyme A biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes CoA from (R)-pantothenate.
Proteins known to be involved in the 5 steps of the subpathway in this organism are:
  1. Pantothenate kinase 4 (Pank4), Pantothenate kinase 3 (Pank3), Pantothenate kinase 1 (Pank1)
  2. Phosphopantothenate--cysteine ligase (Ppcs)
  3. Phosphopantothenoylcysteine decarboxylase (Ppcdc)
  4. Bifunctional coenzyme A synthase (Coasy)
  5. Bifunctional coenzyme A synthase (Coasy)
This subpathway is part of the pathway coenzyme A biosynthesis, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes CoA from (R)-pantothenate, the pathway coenzyme A biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei367 – 3671Acetyl-CoABy similarity
Binding sitei370 – 3701Acetyl-CoABy similarity
Binding sitei382 – 3821Acetyl-CoABy similarity

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • pantothenate kinase activity Source: MGI

GO - Biological processi

  • coenzyme A biosynthetic process Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Coenzyme A biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi2.7.1.33. 3474.
ReactomeiR-MMU-196783. Coenzyme A biosynthesis.
SABIO-RKQ8K4K6.
UniPathwayiUPA00241; UER00352.

Names & Taxonomyi

Protein namesi
Recommended name:
Pantothenate kinase 1 (EC:2.7.1.33)
Short name:
mPank
Short name:
mPank1
Alternative name(s):
Pantothenic acid kinase 1
Gene namesi
Name:Pank1
Synonyms:Pank
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 19

Organism-specific databases

MGIiMGI:1922985. Pank1.

Subcellular locationi

GO - Cellular componenti

  • cell periphery Source: MGI
  • clathrin coat Source: MGI
  • cytosol Source: MGI
  • nucleus Source: MGI
  • recycling endosome Source: MGI
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 548548Pantothenate kinase 1PRO_0000161803Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei30 – 301PhosphoserineCombined sources
Modified residuei165 – 1651PhosphoserineCombined sources

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ8K4K6.
MaxQBiQ8K4K6.
PaxDbiQ8K4K6.
PRIDEiQ8K4K6.

PTM databases

iPTMnetiQ8K4K6.
PhosphoSiteiQ8K4K6.

Expressioni

Tissue specificityi

Expressed in liver and kidney. Isoform 1 is highly expressed in heart and skeletal muscle, whereas isoform 2 is expressed exclusively in testis.

Gene expression databases

BgeeiQ8K4K6.
CleanExiMM_PANK1.
ExpressionAtlasiQ8K4K6. baseline and differential.
GenevisibleiQ8K4K6. MM.

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000043562.

Structurei

3D structure databases

ProteinModelPortaliQ8K4K6.
SMRiQ8K4K6. Positions 182-544.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domaini

The N-terminal extension, present in isoform 1 may be the regulatory domain.

Sequence similaritiesi

Belongs to the type II pantothenate kinase family.Curated

Phylogenomic databases

eggNOGiKOG2201. Eukaryota.
COG5146. LUCA.
GeneTreeiENSGT00390000020719.
HOGENOMiHOG000194690.
HOVERGENiHBG053495.
InParanoidiQ8K4K6.
KOiK09680.
OMAiWGCAGIP.
OrthoDBiEOG7R2BJR.
PhylomeDBiQ8K4K6.
TreeFamiTF314866.

Family and domain databases

InterProiIPR004567. Type_II_PanK.
[Graphical view]
PfamiPF03630. Fumble. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00555. panK_eukar. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8K4K6-1) [UniParc]FASTAAdd to basket

Also known as: PanK1-alpha

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MRGRRAPRPG STEVPAAARD ADTLRARAAS PVRGAQLAED VGTPTGGGEE
60 70 80 90 100
RRGHQLPTAA PRLRESKPQG GSEDRGTADR DLQRGCQSRS PRTAPPVPGM
110 120 130 140 150
GDRGAQHERA ALQSPGAPEG AAAAVNGLLH NGFHPSAASS RDPPAPRFQL
160 170 180 190 200
PSELQPQPLF AQHDSPAKKC RLRRRMDSGR KNRPPFPWFG MDIGGTLVKL
210 220 230 240 250
VYFEPKDITA EEEQEEVENL KSIRKYLTSN TAYGKTGIRD VHLELKNLTM
260 270 280 290 300
CGRKGNLHFI RFPTCAMHLF IQMGSEKNFS SLHTTLCATG GGAFKFEEDF
310 320 330 340 350
RMIADLQLHK LDELDCLIQG LLYVDSVGFN GKPECYYFEN PTNPELCQKK
360 370 380 390 400
PYCLDNPYPM LLVNMGSGVS ILAVYSKDNY KRVTGTSLGG GTFLGLCCLL
410 420 430 440 450
TGCETFEEAL DMAAKGDSTN VDKLVKDIYG GDYERFGLQG SAVASSFGNM
460 470 480 490 500
MSKEKRESIS KEDLARATLV TITNNIGSIA RMCALNENID RVVFVGNFLR
510 520 530 540
INMVSMKLLA YAMDFWSKGQ LKALFLEHEG YFGAVGALLE LFKMTDTQ
Length:548
Mass (Da):60,091
Last modified:October 1, 2002 - v1
Checksum:iC916709D048E429B
GO
Isoform 2 (identifier: Q8K4K6-2) [UniParc]FASTAAdd to basket

Also known as: PanK1-beta

The sequence of this isoform differs from the canonical sequence as follows:
     1-175: Missing.
     176-185: MDSGRKNRPP → MKLVNGRKQT

Show »
Length:373
Mass (Da):41,642
Checksum:i0BCDD8CE29000016
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 175175Missing in isoform 2. 2 PublicationsVSP_004522Add
BLAST
Alternative sequencei176 – 18510MDSGRKNRPP → MKLVNGRKQT in isoform 2. 2 PublicationsVSP_004523

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF200357 mRNA. Translation: AAF23952.1.
AF347700 mRNA. Translation: AAM77216.1.
AK017345 mRNA. Translation: BAB30700.1.
BC023496 mRNA. Translation: AAH23496.1.
CCDSiCCDS37966.1. [Q8K4K6-2]
CCDS50424.1. [Q8K4K6-1]
RefSeqiNP_001107811.1. NM_001114339.2. [Q8K4K6-1]
NP_076281.1. NM_023792.2. [Q8K4K6-2]
UniGeneiMm.24742.

Genome annotation databases

EnsembliENSMUST00000036584; ENSMUSP00000043562; ENSMUSG00000033610. [Q8K4K6-1]
ENSMUST00000112460; ENSMUSP00000108079; ENSMUSG00000033610. [Q8K4K6-2]
GeneIDi75735.
KEGGimmu:75735.
UCSCiuc008hgw.3. mouse. [Q8K4K6-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF200357 mRNA. Translation: AAF23952.1.
AF347700 mRNA. Translation: AAM77216.1.
AK017345 mRNA. Translation: BAB30700.1.
BC023496 mRNA. Translation: AAH23496.1.
CCDSiCCDS37966.1. [Q8K4K6-2]
CCDS50424.1. [Q8K4K6-1]
RefSeqiNP_001107811.1. NM_001114339.2. [Q8K4K6-1]
NP_076281.1. NM_023792.2. [Q8K4K6-2]
UniGeneiMm.24742.

3D structure databases

ProteinModelPortaliQ8K4K6.
SMRiQ8K4K6. Positions 182-544.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000043562.

PTM databases

iPTMnetiQ8K4K6.
PhosphoSiteiQ8K4K6.

Proteomic databases

EPDiQ8K4K6.
MaxQBiQ8K4K6.
PaxDbiQ8K4K6.
PRIDEiQ8K4K6.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000036584; ENSMUSP00000043562; ENSMUSG00000033610. [Q8K4K6-1]
ENSMUST00000112460; ENSMUSP00000108079; ENSMUSG00000033610. [Q8K4K6-2]
GeneIDi75735.
KEGGimmu:75735.
UCSCiuc008hgw.3. mouse. [Q8K4K6-1]

Organism-specific databases

CTDi53354.
MGIiMGI:1922985. Pank1.

Phylogenomic databases

eggNOGiKOG2201. Eukaryota.
COG5146. LUCA.
GeneTreeiENSGT00390000020719.
HOGENOMiHOG000194690.
HOVERGENiHBG053495.
InParanoidiQ8K4K6.
KOiK09680.
OMAiWGCAGIP.
OrthoDBiEOG7R2BJR.
PhylomeDBiQ8K4K6.
TreeFamiTF314866.

Enzyme and pathway databases

UniPathwayiUPA00241; UER00352.
BRENDAi2.7.1.33. 3474.
ReactomeiR-MMU-196783. Coenzyme A biosynthesis.
SABIO-RKQ8K4K6.

Miscellaneous databases

ChiTaRSiPank1. mouse.
PROiQ8K4K6.
SOURCEiSearch...

Gene expression databases

BgeeiQ8K4K6.
CleanExiMM_PANK1.
ExpressionAtlasiQ8K4K6. baseline and differential.
GenevisibleiQ8K4K6. MM.

Family and domain databases

InterProiIPR004567. Type_II_PanK.
[Graphical view]
PfamiPF03630. Fumble. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00555. panK_eukar. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Pantothenate kinase regulation of the intracellular concentration of coenzyme A."
    Rock C.O., Calder R.B., Karim M.A., Jackowski S.
    J. Biol. Chem. 275:1377-1383(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), CHARACTERIZATION.
  2. "The murine pantothenate kinase (Pank1) gene encodes two differentially regulated pantothenate kinase isozymes."
    Rock C.O., Karim M.A., Zhang Y.M., Jackowski S.
    Gene 291:35-43(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), CHARACTERIZATION, ALTERNATIVE SPLICING.
  3. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Strain: C57BL/6J.
    Tissue: Head.
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    Tissue: Liver and Skeletal muscle.
  5. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-30 AND SER-165, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brain, Brown adipose tissue, Kidney and Liver.

Entry informationi

Entry nameiPANK1_MOUSE
AccessioniPrimary (citable) accession number: Q8K4K6
Secondary accession number(s): Q9D3K1, Q9QXM8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 17, 2003
Last sequence update: October 1, 2002
Last modified: June 8, 2016
This is version 118 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.