Q8K4K3 (TRIB2_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Tribbles homolog 2
Short name=TRB-2

Gene names

Name:

Trib2

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	343 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Interacts with MAPK kinases and regulates activation of MAP kinases. Does not display kinase activity By similarity. UniProtKB Q28283 UniProtKB Q96RU8
Subcellular location	Cytoplasm By similarity. Cytoplasm › cytoskeleton By similarity. Note: May associate with the cytoskeleton By similarity.
Domain	The protein kinase domain is predicted to be catalytically inactive.
Sequence similarities	Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. Tribbles subfamily. Contains 1 protein kinase domain.

Ontologies

Keywords
Cellular component	Cytoplasm Cytoskeleton
Molecular function	Protein kinase inhibitor
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	negative regulation of fat cell differentiation Inferred from mutant phenotype PubMed 17576771. Source: BHF-UCL negative regulation of interleukin-10 biosynthetic process Inferred from electronic annotation. Source: Compara negative regulation of protein kinase activity Inferred from electronic annotation. Source: GOC negative regulation of sequence-specific DNA binding transcription factor activity Inferred by curator PubMed 20410507. Source: BHF-UCL positive regulation of proteasomal ubiquitin-dependent protein catabolic process Inferred from direct assay PubMed 20410507. Source: BHF-UCL positive regulation of ubiquitin-protein ligase activity Inferred by curator PubMed 20410507. Source: BHF-UCL regulation of MAP kinase activity Inferred from sequence or structural similarity. Source: UniProtKB
Cellular_component	cytoplasm Inferred from sequence or structural similarity. Source: UniProtKB cytoskeleton Inferred from electronic annotation. Source: UniProtKB-SubCell nucleus Inferred from electronic annotation. Source: Compara
Molecular_function	protein kinase inhibitor activity Inferred from electronic annotation. Source: UniProtKB-KW transcription factor binding Inferred from direct assay PubMed 17576771PubMed 20410507. Source: BHF-UCL transferase activity, transferring phosphorus-containing groups Inferred from electronic annotation. Source: InterPro ubiquitin protein ligase binding Inferred from direct assay PubMed 20410507. Source: BHF-UCL ubiquitin-protein ligase regulator activity Inferred from direct assay PubMed 20410507. Source: BHF-UCL
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 343	343	Tribbles homolog 2		PRO_0000131864
Regions
	Domain	61 – 308	248	Protein kinase
Experimental info
	Sequence conflict	244	1	L → V in AAM45477. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q8K4K3 [UniParc]. Last modified December 6, 2005. Version 2. Checksum: 9418B7AC19FCC23F	FASTA	343	38,773
	10 20 30 40 50 60 MNIHRSTPIT IARYGRSRNK TQDFEELSSI RSAEPSQSFS PNLGSPSPPE TPNLSHCVSC 70 80 90 100 110 120 IGKYLLLEPL EGDHVFRAVH LHSGEELVCK VFEISCYQES LAPCFCLSAH SNINQITEIL 130 140 150 160 170 180 LGETKAYVFF ERSYGDMHSF VRTCKKLREE EAARLFYQIA SAVAHCHDGG LVLRDLKLRK 190 200 210 220 230 240 FIFKDEERTR VKLESLEDAY ILRGDDDSLS DKHGCPAYVS PEILNTSGSY SGKAADVWSL 250 260 270 280 290 300 GVMLYTMLVG RYPFHDIEPS SLFSKIRRGQ FNIPETLSPK AKCLIRSILR REPSERLTSQ 310 320 330 340 EILDHPWFST DFSVSNSGFG AKEACDQLVP DVNMEENLDP FFN

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Mammalian homologs of Drosophila tribbles (htrb) control mitogen activated protein kinase signaling." Kiss-Toth E., Dempsey C., Jozsa V., Caunt J., Oxley K.M., Bagstaff S.M., Wyllie D.H., Harte M., O'Neill L.A.J., Qwarnstrom E.E., Dower S.K. Submitted (MAR-2001) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: C57BL/6J. Tissue: Aorta, Cerebellum, Colon, Retina and Vein.
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: FVB/N. Tissue: Eye, Kidney and Mammary gland.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF358867 mRNA. Translation: AAM45477.1. AK044747 mRNA. Translation: BAC32063.1. AK080064 mRNA. Translation: BAC37820.1. AK082329 mRNA. Translation: BAC38467.1. AK144192 mRNA. Translation: BAE25758.1. BC027159 mRNA. Translation: AAH27159.1. BC034338 mRNA. Translation: AAH34338.1. BC037387 mRNA. Translation: AAH37387.1.
IPI	IPI00320961.
RefSeq	NP_653134.2. NM_144551.5.
UniGene	Mm.266679.
3D structure databases
ProteinModelPortal	Q8K4K3.
SMR	Q8K4K3. Positions 61-341.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q8K4K3. 1 interaction.
STRING	10090.ENSMUSP00000020922.
PTM databases
PhosphoSite	Q8K4K3.
Proteomic databases
PRIDE	Q8K4K3.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000020922; ENSMUSP00000020922; ENSMUSG00000020601.
GeneID	217410.
KEGG	mmu:217410.
UCSC	uc007nbq.1. mouse.
Organism-specific databases
CTD	28951.
MGI	MGI:2145021. Trib2.
Phylogenomic databases
eggNOG	COG0515.
GeneTree	ENSGT00700000104008.
HOGENOM	HOG000231872.
HOVERGEN	HBG067729.
InParanoid	Q8K4K3.
KO	K08814.
OMA	HCHDNGL.
OrthoDB	EOG4WSW9Z.
Gene expression databases
Bgee	Q8K4K3.
CleanEx	MM_TRIB2.
Genevestigator	Q8K4K3.
GermOnline	ENSMUSG00000020601. Mus musculus.
Family and domain databases
InterPro	IPR011009. Kinase-like_dom. IPR000719. Prot_kinase_cat_dom. IPR024104. Tribbles/Ser_Thr_kinase_40. [Graphical view]
PANTHER	PTHR22961. PTHR22961. 1 hit.
Pfam	PF00069. Pkinase. 1 hit. [Graphical view]
SUPFAM	SSF56112. Kinase_like. 1 hit.
PROSITE	PS50011. PROTEIN_KINASE_DOM. 1 hit. [Graphical view]
ProtoNet	Search...
Other
ChiTaRS	TRIB2. mouse.
NextBio	375849.
SOURCE	Search...

Entry information

Entry name

TRIB2_MOUSE

Accession

Primary (citable) accession number: Q8K4K3
Secondary accession number(s): Q8K017, Q8R2V8

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 6, 2005
Last sequence update:	December 6, 2005
Last modified:	May 1, 2013
This is version 91 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program