Q8K4J6 (MKL1_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
MKL/myocardin-like protein 1

Alternative name(s):
Myocardin-related transcription factor A
Short name=MRTF-A
Megakaryoblastic leukemia 1 protein homolog
Basic SAP coiled-coil transcription activator

Gene names

Name:	Mkl1
Synonyms:	Bsac

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	964 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Transcriptional coactivator of serum response factor (SRF) with the potential to modulate SRF target genes. Suppresses TNF-induced cell death by inhibiting activation of caspases; its transcriptional activity is indispensable for the antiapoptotic function. It may up-regulate antiapoptotic molecules, which in turn inhibit caspase activation. Ref.5
Subunit structure	Forms with SCAI and SRF a nuclear ternary complex which binds the CArG consensus motif (CArG box) on DNA via SRF. Some authors (Ref.1) have found contradictory results, they could not demonstrate that it forms a complex with the CArG boxes, even in the presence of SRF. Interacts with ACTB; interaction with ACTB prevents interaction with SCAI. Interacts with MKL2. Ref.5
Subcellular location	Nucleus Ref.5.
Tissue specificity	Expressed in heart, brain, spleen, lung, liver, muscle, kidney and testis.
Developmental stage	Detected throughout the embryo at 10.5 dpc; higher expression is found at 13.5 dpc in neural mesenchymal cells, skeletal muscle of the tongue, and epithelial cells of the colon and small intestine; at 15.5 dpc, expression in epithelial cells of lung, kidney, bladder, and colon is also detected.
Domain	The N-terminal region is required for nuclear localization and the C-terminal region mediates transcriptional activity.
Sequence similarities	Contains 3 RPEL repeats. Contains 1 SAP domain.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Nucleus
Coding sequence diversity	Alternative splicing
Domain	Coiled coil Repeat
PTM	Phosphoprotein
Technical term	3D-structure
Gene Ontology (GO)
Biological process	anti-apoptosis Ref.1 Inferred from direct assay. Source: MGI transcription Ref.1 Inferred from direct assay. Source: MGI
Cellular component	cytoplasm Inferred from direct assay. Source: MGI nucleus Ref.1 Inferred from direct assay. Source: MGI
Molecular function	actin monomer binding Ref.5 Inferred from direct assay. Source: UniProtKB nucleic acid binding Inferred from electronic annotation. Source: InterPro transcription coactivator activity Ref.5 Inferred from direct assay. Source: UniProtKB
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q8K4J6-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q8K4J6-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-35: Missing.

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 964

964

MKL/myocardin-like protein 1

PRO_0000126626

Regions

Repeat

15 – 40

26

RPEL 1

Repeat

59 – 84

26

RPEL 2

Repeat

103 – 128

26

RPEL 3

Domain

385 – 419

35

SAP

Region

1 – 291

291

Mediates interaction with SCAI and ACTB

Coiled coil

552 – 600

49

Potential

Compositional bias

299 – 325

27

Gln-rich

Compositional bias

712 – 844

133

Pro-rich

Amino acid modifications

Modified residue

41

1

Phosphoserine By similarity

Modified residue

488

1

Phosphothreonine By similarity

Modified residue

492

1

Phosphoserine By similarity

Natural variations

Alternative sequence

1 – 35

35

Missing in isoform 2.

VSP_007652

Experimental info

Sequence conflict

53

1

E → Q in BAC31809. Ref.3

Sequence conflict

724

1

S → D in BAC40873. Ref.3

Sequence conflict

728

1

S → F in AAM94258. Ref.1

Secondary structure

1

.

.

.

.

.

964

Helix Strand Turn

Details...

Helix

59 – 66

8

Helix

71 – 76

6

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified June 27, 2003. Version 2. Checksum: AFAEA328A1860CE5	FASTA	964	102,546
	10 20 30 40 50 60 MTLLEPEMLM MAVQSVLQLK LQQRRTREEL VSQGIMPPLK SPAAFHEQRR SLERARTEDY 70 80 90 100 110 120 LKRKIRSRPE RAELVRMHIL EETSAEPSLQ AKQLKLKRAR LADDLNEKIA QRPGPMELVE 130 140 150 160 170 180 KNILPVESSL KEAIIVGQVN YPKVADSSSF DEDSSDALSP EQPASHESQG SVPSPLESRV 190 200 210 220 230 240 SDPLPSATSI SPTQVLSQLP MAPDPGETLF LAEQPPLPPA PLLPPSLANG SIVPTAKPAP 250 260 270 280 290 300 TLIKQSQPKS ASEKSQRSKK AKELKPKVKK LKYHQYIPPD QKQDKGAPAM DSSYAKILQQ 310 320 330 340 350 360 QQLFLQLQIL NQQQQQQQQQ HYNYQAILPA PPKPSAETPG SSAPTPSRSL STSSSPSSGT 370 380 390 400 410 420 PGPSGLARQS STALAAKPGA LPANLDDMKV AELKQELKLR SLPVSGTKTE LIERLRAYQD 430 440 450 460 470 480 QVSPAPGAPK APATTSVLSK AGEVVVAFPA ALLSTGSALV TAGLAPAEMV VATVTSNGMV 490 500 510 520 530 540 KFGSTGSTPP VSPTPSERSL LSTGDENSTP GDAFGEMVTS PLTQLTLQAS PLQIVKEEGA 550 560 570 580 590 600 RAASCCLSPG ARAELEGLDK DQMLQEKDKQ IEELTRMLQQ KQQLVELLRL QLEQQKRAQQ 610 620 630 640 650 660 PAPASSPVKR ESGFSSCQLS CQPQGSAHAF GSGLVVPTTN HGDTQAPAPE SPPVVVKQEA 670 680 690 700 710 720 GPPEPDLAPS SQLLLGSQGT SFLKRVSPPT LVTDSTGTHL ILTVTNKSAD GPGLPAGSPQ 730 740 750 760 770 780 QPLSQPGSPA PGPPAQMDLE HPPQPPFATP TSLLKKEPPG YEETVTQQPK QQENGSSSQH 790 800 810 820 830 840 MDDLFDILIQ SGEISADFKE PPSLPGKEKS PPAAAAYGPP LTPQPSPLSE LPQAAPPPGS 850 860 870 880 890 900 PTLPGRLEDF LESSTGLPLL TSGHEGPEPL SLIDDLHSQM LSSSAILDHP PSPMDTSELH 910 920 930 940 950 960 FAPEPSSGMG LDLAVGHLDS MDWLELSSGG PVLSLAPLST AAPSLFSMDF LDGHDLQLHW DSCL
	Isoform 2. Checksum: B13D0985013B6E49	FASTA	929	98,463
	10 20 30 40 50 60 MPPLKSPAAF HEQRRSLERA RTEDYLKRKI RSRPERAELV RMHILEETSA EPSLQAKQLK 70 80 90 100 110 120 LKRARLADDL NEKIAQRPGP MELVEKNILP VESSLKEAII VGQVNYPKVA DSSSFDEDSS 130 140 150 160 170 180 DALSPEQPAS HESQGSVPSP LESRVSDPLP SATSISPTQV LSQLPMAPDP GETLFLAEQP 190 200 210 220 230 240 PLPPAPLLPP SLANGSIVPT AKPAPTLIKQ SQPKSASEKS QRSKKAKELK PKVKKLKYHQ 250 260 270 280 290 300 YIPPDQKQDK GAPAMDSSYA KILQQQQLFL QLQILNQQQQ QQQQQHYNYQ AILPAPPKPS 310 320 330 340 350 360 AETPGSSAPT PSRSLSTSSS PSSGTPGPSG LARQSSTALA AKPGALPANL DDMKVAELKQ 370 380 390 400 410 420 ELKLRSLPVS GTKTELIERL RAYQDQVSPA PGAPKAPATT SVLSKAGEVV VAFPAALLST 430 440 450 460 470 480 GSALVTAGLA PAEMVVATVT SNGMVKFGST GSTPPVSPTP SERSLLSTGD ENSTPGDAFG 490 500 510 520 530 540 EMVTSPLTQL TLQASPLQIV KEEGARAASC CLSPGARAEL EGLDKDQMLQ EKDKQIEELT 550 560 570 580 590 600 RMLQQKQQLV ELLRLQLEQQ KRAQQPAPAS SPVKRESGFS SCQLSCQPQG SAHAFGSGLV 610 620 630 640 650 660 VPTTNHGDTQ APAPESPPVV VKQEAGPPEP DLAPSSQLLL GSQGTSFLKR VSPPTLVTDS 670 680 690 700 710 720 TGTHLILTVT NKSADGPGLP AGSPQQPLSQ PGSPAPGPPA QMDLEHPPQP PFATPTSLLK 730 740 750 760 770 780 KEPPGYEETV TQQPKQQENG SSSQHMDDLF DILIQSGEIS ADFKEPPSLP GKEKSPPAAA 790 800 810 820 830 840 AYGPPLTPQP SPLSELPQAA PPPGSPTLPG RLEDFLESST GLPLLTSGHE GPEPLSLIDD 850 860 870 880 890 900 LHSQMLSSSA ILDHPPSPMD TSELHFAPEP SSGMGLDLAV GHLDSMDWLE LSSGGPVLSL 910 920 APLSTAAPSL FSMDFLDGHD LQLHWDSCL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Identification of a novel transcriptional activator, BSAC, by a functional cloning to inhibit tumor necrosis factor-induced cell death." Sasazuki T., Sawada Y., Sakon S., Kitamura T., Kishi T., Okazaki T., Katano M., Tanaka M., Watanabe M., Yagita H., Okumura K., Nakano H. J. Biol. Chem. 277:28853-28860(2002) [PubMed: 12019265] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1). Tissue: Spleen.
[2]	"Potentiation of serum response factor activity by a family of myocardin-related transcription factors." Wang D.-Z., Li S., Hockemeyer D., Sutherland L., Wang Z., Schratt G., Richardson J.A., Nordheim A., Olson E.N. Proc. Natl. Acad. Sci. U.S.A. 99:14855-14860(2002) [PubMed: 12397177] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2). Strain: C57BL/6.
[3]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed: 16141072] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Strain: C57BL/6J. Tissue: Brain cortex.
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Strain: C57BL/6. Tissue: Brain.
[5]	"SCAI acts as a suppressor of cancer cell invasion through the transcriptional control of beta1-integrin." Brandt D.T., Baarlink C., Kitzing T.M., Kremmer E., Ivaska J., Nollau P., Grosse R. Nat. Cell Biol. 11:557-568(2009) [PubMed: 19350017] [Abstract] Cited for: FUNCTION, INTERACTION WITH ACTB; SCAI AND SRF, SUBCELLULAR LOCATION.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AF385582 mRNA. Translation: AAM94258.1.
AF532597 mRNA. Translation: AAN33041.1.
AK044188 mRNA. Translation: BAC31809.1.
AK089416 mRNA. Translation: BAC40873.1.
BC050941 mRNA. Translation: AAH50941.1.

IPI

IPI00276393.
IPI00331708.

RefSeq

NP_001076005.1.
NP_694629.2.

UniGene

Mm.439814.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2V51	X-ray	2.35	E/F	16-41	[»]
2V52	X-ray	1.45	M	54-85	[»]

ProteinModelPortal

Q8K4J6.

SMR

Q8K4J6. Positions 377-423.

ModBase

Search...

Protein-protein interaction databases

STRING

Q8K4J6.

PTM databases

PhosphoSite

Q8K4J6.

Proteomic databases

PRIDE

Q8K4J6.

Genome annotation databases

Ensembl

ENSMUST00000109579; ENSMUSP00000105207; ENSMUSG00000042292; Mus musculus. [Genome view]

GeneID

223701.

KEGG

mmu:223701.

UCSC

uc007wwc.1. mouse.
uc007wwd.1. mouse.

Organism-specific databases

CTD

223701.

MGI

MGI:2384495. Mkl1.

Phylogenomic databases

HOGENOM

HBG443837.

HOVERGEN

HBG036493.

InParanoid

Q8K4J6.

OMA

SADFKEP.

PhylomeDB

Q8K4J6.

Gene expression databases

ArrayExpress

Q8K4J6.

Bgee

Q8K4J6.

CleanEx

MM_MKL1.

Genevestigator

Q8K4J6.

GermOnline

ENSMUSG00000042292. Mus musculus.

Family and domain databases

InterPro

IPR004018. RPEL_repeat.
IPR003034. SAP_DNA-bd.
[Graphical view]

Pfam

PF02755. RPEL. 3 hits.
PF02037. SAP. 1 hit.
[Graphical view]

SMART

SM00707. RPEL. 3 hits.
SM00513. SAP. 1 hit.
[Graphical view]

PROSITE

PS51073. RPEL. 3 hits.
PS50800. SAP. 1 hit.
[Graphical view]

ProtoNet

Search...

Other Resources

NextBio

376834.

SOURCE

Search...

Entry information

Entry name

MKL1_MOUSE

Accession

Primary (citable) accession number: Q8K4J6
Secondary accession number(s): Q642U1

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 27, 2003
Last sequence update:	June 27, 2003
Last modified:	August 10, 2010
This is version 79 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)