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UniProtKB - Q8K4J6 (MKL1_MOUSE)

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Protein

MKL/myocardin-like protein 1

Gene

Mkl1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Transcriptional coactivator of serum response factor (SRF) with the potential to modulate SRF target genes. Suppresses TNF-induced cell death by inhibiting activation of caspases; its transcriptional activity is indispensable for the antiapoptotic function. It may up-regulate antiapoptotic molecules, which in turn inhibit caspase activation.1 Publication

GO - Molecular functioni

  • actin binding Source: MGI
  • actin monomer binding Source: UniProtKB
  • leucine zipper domain binding Source: MGI
  • RNA polymerase II transcription coactivator activity Source: GO_Central
  • transcription coactivator activity Source: UniProtKB
  • transcription factor activity, sequence-specific DNA binding Source: MGI
  • transcription regulatory region sequence-specific DNA binding Source: MGI
Complete GO annotation...

GO - Biological processi

  • actin cytoskeleton organization Source: MGI
  • forebrain development Source: MGI
  • negative regulation of apoptotic signaling pathway Source: MGI
  • negative regulation of cysteine-type endopeptidase activity involved in apoptotic process Source: MGI
  • neuron migration Source: MGI
  • neuron projection development Source: MGI
  • positive regulation of transcription, DNA-templated Source: MGI
  • positive regulation of transcription from RNA polymerase II promoter Source: UniProtKB
  • positive regulation of transcription via serum response element binding Source: UniProtKB
  • smooth muscle cell differentiation Source: MGI
Complete GO annotation...

Keywordsi

Molecular functionActin-binding
Biological processTranscription, Transcription regulation

Enzyme and pathway databases

ReactomeiR-MMU-5663220. RHO GTPases Activate Formins.

Names & Taxonomyi

Protein namesi
Recommended name:
MKL/myocardin-like protein 1
Alternative name(s):
Basic SAP coiled-coil transcription activator
Megakaryoblastic leukemia 1 protein homolog
Myocardin-related transcription factor A
Short name:
MRTF-A
Gene namesi
Name:Mkl1
Synonyms:Bsac
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus
Proteomesi
  • UP000000589 Componenti: Chromosome 15

Organism-specific databases

MGIiMGI:2384495. Mkl1.

Subcellular locationi

  • Cytoplasm
  • Nucleus

    • Note: Binding to globular actin is required to maintain cytoplasmic localization. Nuclear localization is regulated by MICAL2, which mediates depolymerization of nuclear globular actin, promoting retention of MKL1 in the nucleus and subsequent formation of an active complex with SRF (By similarity).By similarity

GO - Cellular componenti

  • cytoplasm Source: MGI
  • cytosol Source: MGI
  • nucleoplasm Source: MGI
  • nucleus Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001266261 – 964MKL/myocardin-like protein 1Add BLAST964

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei41PhosphoserineBy similarity1
Modified residuei191PhosphoserineBy similarity1
Modified residuei371PhosphoserineBy similarity1
Modified residuei423PhosphoserineBy similarity1
Modified residuei488PhosphothreonineCombined sources1
Modified residuei492PhosphoserineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ8K4J6.
PaxDbiQ8K4J6.
PRIDEiQ8K4J6.

PTM databases

iPTMnetiQ8K4J6.
PhosphoSitePlusiQ8K4J6.

Expressioni

Tissue specificityi

Expressed in heart, brain, spleen, lung, liver, muscle, kidney and testis.

Developmental stagei

Detected throughout the embryo at 10.5 dpc; higher expression is found at 13.5 dpc in neural mesenchymal cells, skeletal muscle of the tongue, and epithelial cells of the colon and small intestine; at 15.5 dpc, expression in epithelial cells of lung, kidney, bladder, and colon is also detected.

Gene expression databases

BgeeiENSMUSG00000042292.
CleanExiMM_MKL1.
ExpressionAtlasiQ8K4J6. baseline and differential.
GenevisibleiQ8K4J6. MM.

Interactioni

Subunit structurei

Forms with SCAI and SRF a nuclear ternary complex which binds the CArG consensus motif (CArG box) on DNA via SRF. Some authors (PubMed:12019265) have found contradictory results, they could not demonstrate that it forms a complex with the CArG boxes, even in the presence of SRF. Interacts with ACTB; interaction with ACTB prevents interaction with SCAI. Interacts with MKL2.3 Publications

Binary interactionsi

Show more details

GO - Molecular functioni

  • actin binding Source: MGI
  • actin monomer binding Source: UniProtKB
  • leucine zipper domain binding Source: MGI
Complete GO annotation...

Protein-protein interaction databases

BioGridi230180. 2 interactors.
DIPiDIP-60884N.
IntActiQ8K4J6. 5 interactors.
STRINGi10090.ENSMUSP00000105207.

Structurei

Secondary structure

1964
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi16 – 23Combined sources8
Helixi27 – 32Combined sources6
Beta strandi39 – 41Combined sources3
Helixi59 – 66Combined sources8
Helixi71 – 76Combined sources6
Helixi87 – 110Combined sources24
Helixi115 – 120Combined sources6

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2V51X-ray2.35E/F16-41[»]
2V52X-ray1.45M54-85[»]
2YJEX-ray3.10M16-142[»]
2YJFX-ray3.50M16-142[»]
ProteinModelPortaliQ8K4J6.
SMRiQ8K4J6.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ8K4J6.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati15 – 40RPEL 1Add BLAST26
Repeati59 – 84RPEL 2Add BLAST26
Repeati103 – 128RPEL 3Add BLAST26
Domaini385 – 419SAPPROSITE-ProRule annotationAdd BLAST35

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 291Mediates interaction with SCAI and ACTB1 PublicationAdd BLAST291

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili552 – 600Sequence analysisAdd BLAST49

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi299 – 325Gln-richAdd BLAST27
Compositional biasi712 – 844Pro-richAdd BLAST133

Domaini

The N-terminal region is required for nuclear localization and the C-terminal region mediates transcriptional activity.
The RPEL repeats mediate binding to globular actin.

Keywords - Domaini

Coiled coil, Repeat

Phylogenomic databases

eggNOGiENOG410IFJX. Eukaryota.
ENOG41101AM. LUCA.
GeneTreeiENSGT00530000063195.
HOGENOMiHOG000038001.
HOVERGENiHBG036493.
InParanoidiQ8K4J6.
OMAiAQMDLEH.
OrthoDBiEOG091G01ZY.
PhylomeDBiQ8K4J6.
TreeFamiTF326024.

Family and domain databases

Gene3Di1.10.720.30. 1 hit.
InterProiView protein in InterPro
IPR029992. MRTF-A.
IPR004018. RPEL_repeat.
IPR003034. SAP_dom.
PANTHERiPTHR22793:SF17. PTHR22793:SF17. 1 hit.
PfamiView protein in Pfam
PF02755. RPEL. 3 hits.
PF02037. SAP. 1 hit.
SMARTiView protein in SMART
SM00707. RPEL. 3 hits.
SM00513. SAP. 1 hit.
SUPFAMiSSF68906. SSF68906. 1 hit.
PROSITEiView protein in PROSITE
PS51073. RPEL. 3 hits.
PS50800. SAP. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8K4J6-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MTLLEPEMLM MAVQSVLQLK LQQRRTREEL VSQGIMPPLK SPAAFHEQRR 
        60         70         80         90        100
SLERARTEDY LKRKIRSRPE RAELVRMHIL EETSAEPSLQ AKQLKLKRAR 
       110        120        130        140        150
LADDLNEKIA QRPGPMELVE KNILPVESSL KEAIIVGQVN YPKVADSSSF 
       160        170        180        190        200
DEDSSDALSP EQPASHESQG SVPSPLESRV SDPLPSATSI SPTQVLSQLP 
       210        220        230        240        250
MAPDPGETLF LAEQPPLPPA PLLPPSLANG SIVPTAKPAP TLIKQSQPKS 
       260        270        280        290        300
ASEKSQRSKK AKELKPKVKK LKYHQYIPPD QKQDKGAPAM DSSYAKILQQ 
       310        320        330        340        350
QQLFLQLQIL NQQQQQQQQQ HYNYQAILPA PPKPSAETPG SSAPTPSRSL 
       360        370        380        390        400
STSSSPSSGT PGPSGLARQS STALAAKPGA LPANLDDMKV AELKQELKLR 
       410        420        430        440        450
SLPVSGTKTE LIERLRAYQD QVSPAPGAPK APATTSVLSK AGEVVVAFPA 
       460        470        480        490        500
ALLSTGSALV TAGLAPAEMV VATVTSNGMV KFGSTGSTPP VSPTPSERSL 
       510        520        530        540        550
LSTGDENSTP GDAFGEMVTS PLTQLTLQAS PLQIVKEEGA RAASCCLSPG 
       560        570        580        590        600
ARAELEGLDK DQMLQEKDKQ IEELTRMLQQ KQQLVELLRL QLEQQKRAQQ 
       610        620        630        640        650
PAPASSPVKR ESGFSSCQLS CQPQGSAHAF GSGLVVPTTN HGDTQAPAPE 
       660        670        680        690        700
SPPVVVKQEA GPPEPDLAPS SQLLLGSQGT SFLKRVSPPT LVTDSTGTHL 
       710        720        730        740        750
ILTVTNKSAD GPGLPAGSPQ QPLSQPGSPA PGPPAQMDLE HPPQPPFATP 
       760        770        780        790        800
TSLLKKEPPG YEETVTQQPK QQENGSSSQH MDDLFDILIQ SGEISADFKE 
       810        820        830        840        850
PPSLPGKEKS PPAAAAYGPP LTPQPSPLSE LPQAAPPPGS PTLPGRLEDF 
       860        870        880        890        900
LESSTGLPLL TSGHEGPEPL SLIDDLHSQM LSSSAILDHP PSPMDTSELH 
       910        920        930        940        950
FAPEPSSGMG LDLAVGHLDS MDWLELSSGG PVLSLAPLST AAPSLFSMDF 
       960 
LDGHDLQLHW DSCL
Length:964
Mass (Da):102,546
Last modified:June 27, 2003 - v2
Checksum:iAFAEA328A1860CE5
GO
Isoform 2 (identifier: Q8K4J6-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-35: Missing.

Show »
Length:929
Mass (Da):98,463
Checksum:iB13D0985013B6E49
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti53E → Q in BAC31809 (PubMed:16141072).Curated1
Sequence conflicti724S → D in BAC40873 (PubMed:16141072).Curated1
Sequence conflicti728S → F in AAM94258 (PubMed:12019265).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0076521 – 35Missing in isoform 2. 3 PublicationsAdd BLAST35

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF385582 mRNA. Translation: AAM94258.1.
AF532597 mRNA. Translation: AAN33041.1.
AK044188 mRNA. Translation: BAC31809.1.
AK089416 mRNA. Translation: BAC40873.1.
BC050941 mRNA. Translation: AAH50941.1.
CCDSiCCDS37147.1. [Q8K4J6-1]
CCDS49674.1. [Q8K4J6-2]
RefSeqiNP_001076005.1. NM_001082536.1. [Q8K4J6-2]
NP_694629.2. NM_153049.3. [Q8K4J6-1]
UniGeneiMm.439814.

Genome annotation databases

EnsembliENSMUST00000109579; ENSMUSP00000105207; ENSMUSG00000042292. [Q8K4J6-1]
ENSMUST00000134469; ENSMUSP00000119530; ENSMUSG00000042292. [Q8K4J6-2]
ENSMUST00000149582; ENSMUSP00000117745; ENSMUSG00000042292. [Q8K4J6-2]
GeneIDi223701.
KEGGimmu:223701.
UCSCiuc007wwc.2. mouse. [Q8K4J6-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiMKL1_MOUSE
AccessioniPrimary (citable) accession number: Q8K4J6
Secondary accession number(s): Q642U1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 27, 2003
Last sequence update: June 27, 2003
Last modified: June 7, 2017
This is version 136 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references