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Protein

Transcription factor COE4

Gene

Ebf4

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Seems to weakly activate transcription. Binds an Olf-1 consensus site in vitro.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri152 – 171C5-typeSequence analysisAdd BLAST20

GO - Molecular functioni

  • DNA binding Source: MGI
  • metal ion binding Source: UniProtKB-KW
  • protein dimerization activity Source: InterPro
  • RNA polymerase II regulatory region sequence-specific DNA binding Source: NTNU_SB
  • transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific DNA binding Source: NTNU_SB
  • transcription factor binding Source: MGI

GO - Biological processi

  • multicellular organism development Source: UniProtKB-KW
  • positive regulation of transcription by RNA polymerase II Source: NTNU_SB
  • regulation of transcription, DNA-templated Source: MGI

Keywordsi

Molecular functionActivator, Developmental protein, DNA-binding
Biological processTranscription, Transcription regulation
LigandMetal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Transcription factor COE4
Alternative name(s):
Early B-cell factor 4
Short name:
EBF-4
Olf-1/EBF-like 4
Short name:
O/E-4
Short name:
OE-4
Gene namesi
Name:Ebf4
Synonyms:Coe4
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 2

Organism-specific databases

MGIiMGI:2385972 Ebf4

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001078361 – 599Transcription factor COE4Add BLAST599

Proteomic databases

PaxDbiQ8K4J2
PRIDEiQ8K4J2

PTM databases

iPTMnetiQ8K4J2
PhosphoSitePlusiQ8K4J2

Expressioni

Tissue specificityi

Expressed in the neuronal and basal cell layers of olfactory epithelium. Absent in the vomeronasal organ.1 Publication

Gene expression databases

BgeeiENSMUSG00000053552
CleanExiMM_EBF4
ExpressionAtlasiQ8K4J2 baseline and differential
GenevisibleiQ8K4J2 MM

Interactioni

Subunit structurei

Forms either a homodimer or a heterodimer with a related family member.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei164Interaction with DNABy similarity1
Sitei173Interaction with DNABy similarity1

GO - Molecular functioni

  • protein dimerization activity Source: InterPro
  • transcription factor binding Source: MGI

Protein-protein interaction databases

BioGridi230746, 2 interactors
STRINGi10090.ENSMUSP00000105915

Structurei

3D structure databases

ProteinModelPortaliQ8K4J2
SMRiQ8K4J2
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini256 – 339IPT/TIGAdd BLAST84

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni64 – 67Interaction with DNABy similarity4
Regioni198 – 205Interaction with DNABy similarity8
Regioni237 – 240Interaction with DNABy similarity4

Sequence similaritiesi

Belongs to the COE family.Curated

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri152 – 171C5-typeSequence analysisAdd BLAST20

Keywords - Domaini

Zinc-finger

Phylogenomic databases

eggNOGiKOG3836 Eukaryota
ENOG410XQ9Z LUCA
GeneTreeiENSGT00390000014051
HOVERGENiHBG005108
InParanoidiQ8K4J2
KOiK09103
TreeFamiTF313391

Family and domain databases

CDDicd01175 IPT_COE, 1 hit
Gene3Di2.60.40.10, 1 hit
2.60.40.3180, 1 hit
4.10.280.10, 2 hits
InterProiView protein in InterPro
IPR032200 COE_DBD
IPR038173 COE_DBD_sf
IPR032201 COE_HLH
IPR038006 COE_IPT
IPR036638 HLH_DNA-bd_sf
IPR013783 Ig-like_fold
IPR014756 Ig_E-set
IPR002909 IPT_dom
IPR003523 Transcription_factor_COE
IPR018350 Transcription_factor_COE_CS
PANTHERiPTHR10747 PTHR10747, 1 hit
PfamiView protein in Pfam
PF16422 COE1_DBD, 1 hit
PF16423 COE1_HLH, 1 hit
PF01833 TIG, 1 hit
SMARTiView protein in SMART
SM00429 IPT, 1 hit
SUPFAMiSSF81296 SSF81296, 1 hit
PROSITEiView protein in PROSITE
PS01345 COE, 1 hit

Sequences (5)i

Sequence statusi: Complete.

This entry describes 5 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 3 (identifier: Q8K4J2-1) [UniParc]FASTAAdd to basket
Also known as: 4-23

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MFPAQDALPR GGLHLKEEPL LPSSLGSVRS WMQSAGILDS NTAAQSGVGL
60 70 80 90 100
ARAHFEKQPP SNLRKSNFFH FVLAMYDRQG QPVEVERTAF IDFVEKDREP
110 120 130 140 150
GTEKTNNGIH YRLRLVYNNG LRTEQDLYVR LIDSMSKQAI IYEGQDKNPE
160 170 180 190 200
MCRVLLTHEI MCSRCCDRKS CGNRNETPSD PVIIDRFFLK FFLKCNQNCL
210 220 230 240 250
KNAGNPRDMR RFQVVVSTTV SVDGHVLAVS DNMFVHNNSK HGRRARRLDP
260 270 280 290 300
SEAATPCIKA ISPGEGWTTG GATVIIIGDN FFDGLQVVFG NVLLWSELIT
310 320 330 340 350
PHAIRVQTPP RHIPGVVEVT LSYKSKQFCK GAPGRFVYTA LNEPTIDYGF
360 370 380 390 400
QRLQKVIPRH PGDPERLPKE VLLKRAADLA EALYGVPSSN QELLLKRAAD
410 420 430 440 450
VAEALYSAPR APAPLGPLAP SHPHPAVVGI NAFSSPLAIA VGDTTPEPGY
460 470 480 490 500
ARSCGSASPR FAPSPGSQQS SYGSGLGAGL GSYGAPGVTG LGVPGSPSFL
510 520 530 540 550
NGSTATSPFA IMPSSPPLAA ASSMSLPAAA PTTSVFSFSP VNMICAVKQR
560 570 580 590
SAFAPVLRPP SSPSQACPRA HREGLPDQPF EDTDKFHSAA RGLQGLAYS
Length:599
Mass (Da):64,624
Last modified:October 1, 2002 - v1
Checksum:i76748B3E04D42260
GO
Isoform 1 (identifier: Q8K4J2-2) [UniParc]FASTAAdd to basket
Also known as: 4-11

The sequence of this isoform differs from the canonical sequence as follows:
     577-582: DQPFED → AQRTGR
     583-599: Missing.

Show »
Length:582
Mass (Da):62,758
Checksum:i22EEC610A093175F
GO
Isoform 2 (identifier: Q8K4J2-3) [UniParc]FASTAAdd to basket
Also known as: 4-14

The sequence of this isoform differs from the canonical sequence as follows:
     481-508: GSYGAPGVTGLGVPGSPSFLNGSTATSP → APRWRLPPPCPFRPPPPPPASSPSRLST
     509-599: Missing.

Show »
Length:508
Mass (Da):55,639
Checksum:iAC940A674C5DE872
GO
Isoform 4 (identifier: Q8K4J2-4) [UniParc]FASTAAdd to basket
Also known as: 4-132

The sequence of this isoform differs from the canonical sequence as follows:
     511-541: IMPSSPPLAAASSMSLPAAAPTTSVFSFSPV → KERLRPCAAPTQFPIAGLPQSPQRGASRPAF
     542-599: Missing.

Note: May be produced at very low levels due to a premature stop codon in the mRNA, leading to nonsense-mediated mRNA decay.
Show »
Length:541
Mass (Da):58,661
Checksum:i18915B375F4C5E1A
GO
Isoform 5 (identifier: Q8K4J2-5) [UniParc]FASTAAdd to basket
Also known as: 4S

The sequence of this isoform differs from the canonical sequence as follows:
     392-426: ELLLKRAADVAEALYSAPRAPAPLGPLAPSHPHPA → VWRLCPPPSARGRGSDPAPAAAPAVPRSCLRRSSS
     427-599: Missing.

Show »
Length:426
Mass (Da):47,445
Checksum:i28C18C1A9B677A2B
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_001119392 – 426ELLLK…HPHPA → VWRLCPPPSARGRGSDPAPA AAPAVPRSCLRRSSS in isoform 5. 1 PublicationAdd BLAST35
Alternative sequenceiVSP_001120427 – 599Missing in isoform 5. 1 PublicationAdd BLAST173
Alternative sequenceiVSP_001121481 – 508GSYGA…TATSP → APRWRLPPPCPFRPPPPPPA SSPSRLST in isoform 2. 1 PublicationAdd BLAST28
Alternative sequenceiVSP_001122509 – 599Missing in isoform 2. 1 PublicationAdd BLAST91
Alternative sequenceiVSP_001123511 – 541IMPSS…SFSPV → KERLRPCAAPTQFPIAGLPQ SPQRGASRPAF in isoform 4. 1 PublicationAdd BLAST31
Alternative sequenceiVSP_001124542 – 599Missing in isoform 4. 1 PublicationAdd BLAST58
Alternative sequenceiVSP_001125577 – 582DQPFED → AQRTGR in isoform 1. 1 Publication6
Alternative sequenceiVSP_001126583 – 599Missing in isoform 1. 1 PublicationAdd BLAST17

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF387630 mRNA Translation: AAM97580.1
AF387631 mRNA Translation: AAM97581.1
AF387632 mRNA Translation: AAM97582.1
AF387633 mRNA Translation: AAM97583.1
AF387634 mRNA Translation: AAM97584.1
BX890605, BX936285 Genomic DNA Translation: CAM20114.1
BX936285, BX890605 Genomic DNA Translation: CAM15942.1
CCDSiCCDS50709.1 [Q8K4J2-1]
RefSeqiNP_001103983.1, NM_001110513.1 [Q8K4J2-1]
XP_011237773.1, XM_011239471.2 [Q8K4J2-2]
XP_011237774.1, XM_011239472.2 [Q8K4J2-3]
UniGeneiMm.32582

Genome annotation databases

EnsembliENSMUST00000110286; ENSMUSP00000105915; ENSMUSG00000053552 [Q8K4J2-1]
ENSMUST00000126740; ENSMUSP00000133528; ENSMUSG00000053552 [Q8K4J2-2]
ENSMUST00000140169; ENSMUSP00000134520; ENSMUSG00000053552 [Q8K4J2-4]
GeneIDi228598
KEGGimmu:228598
UCSCiuc008miq.2 mouse [Q8K4J2-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiCOE4_MOUSE
AccessioniPrimary (citable) accession number: Q8K4J2
Secondary accession number(s): A2BI82
, A2BI83, Q8K4J1, Q8K4J3, Q8K4J4, Q8K4J5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 28, 2002
Last sequence update: October 1, 2002
Last modified: March 28, 2018
This is version 115 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health