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Protein

Protein artemis

Gene

Dclre1c

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Required for V(D)J recombination, the process by which exons encoding the antigen-binding domains of immunoglobulins and T-cell receptor proteins are assembled from individual V, (D), and J gene segments. V(D)J recombination is initiated by the lymphoid specific RAG endonuclease complex, which generates site specific DNA double strand breaks (DSBs). These DSBs present two types of DNA end structures: hairpin sealed coding ends and phosphorylated blunt signal ends. These ends are independently repaired by the non homologous end joining (NHEJ) pathway to form coding and signal joints respectively. This protein likely exhibits single-strand specific 5'-3' exonuclease activity in isolation, and may acquire endonucleolytic activity on 5' and 3' hairpins and overhangs when in a complex with PRKDC. The latter activity may be required specifically for the resolution of closed hairpins prior to the formation of the coding joint. May also be required for the repair of complex DSBs induced by ionizing radiation, which require substantial end-processing prior to religation by NHEJ.3 Publications

GO - Molecular functioni

GO - Biological processi

  • adaptive immune response Source: UniProtKB-KW
  • B cell differentiation Source: MGI
  • chromosome organization Source: MGI
  • DNA recombination Source: MGI
  • double-strand break repair Source: MGI
  • nucleic acid phosphodiester bond hydrolysis Source: MGI
  • response to ionizing radiation Source: MGI
  • telomere maintenance Source: MGI
  • V(D)J recombination Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Endonuclease, Exonuclease, Hydrolase, Nuclease

Keywords - Biological processi

Adaptive immunity, DNA damage, DNA recombination, DNA repair, Immunity

Keywords - Ligandi

Magnesium

Enzyme and pathway databases

ReactomeiR-MMU-5693571. Nonhomologous End-Joining (NHEJ).

Names & Taxonomyi

Protein namesi
Recommended name:
Protein artemis (EC:3.1.-.-)
Short name:
mArt
Alternative name(s):
DNA cross-link repair 1C protein
SNM1-like protein
Gene namesi
Name:Dclre1c
Synonyms:Art, Snm1l
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 2

Organism-specific databases

MGIiMGI:2441769. Dclre1c.

Subcellular locationi

GO - Cellular componenti

  • nonhomologous end joining complex Source: UniProtKB
  • nucleus Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Keywords - Diseasei

SCID

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 705705Protein artemisPRO_0000209124Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei380 – 3801PhosphothreonineCombined sources
Modified residuei385 – 3851PhosphoserineCombined sources
Modified residuei658 – 6581Phosphoserine; by ATMBy similarity

Post-translational modificationi

Phosphorylation on undefined residues by PRKDC may stimulate endonucleolytic activity on 5' and 3' hairpins and overhangs. PRKDC must remain present, even after phosphorylation, for efficient hairpin opening. Also phosphorylated by ATM in response to ionizing radiation (IR) and by ATR in response to ultraviolet (UV) radiation (By similarity).By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ8K4J0.
PRIDEiQ8K4J0.

PTM databases

iPTMnetiQ8K4J0.
PhosphoSiteiQ8K4J0.

Expressioni

Gene expression databases

BgeeiQ8K4J0.
ExpressionAtlasiQ8K4J0. baseline and differential.
GenevisibleiQ8K4J0. MM.

Interactioni

Subunit structurei

Interacts with ATM, BRCA1, PRKDC and TP53BP1. Also exhibits ATM- and phosphorylation-dependent interaction with the MRN complex, composed of MRE11A/MRE11, RAD50, and NBN (By similarity).By similarity

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000100053.

Structurei

3D structure databases

ProteinModelPortaliQ8K4J0.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG1361. Eukaryota.
COG1236. LUCA.
GeneTreeiENSGT00530000063183.
HOGENOMiHOG000231568.
HOVERGENiHBG081421.
InParanoidiQ8K4J0.
KOiK10887.
OrthoDBiEOG70CR8F.
PhylomeDBiQ8K4J0.
TreeFamiTF329572.

Family and domain databases

Gene3Di3.60.15.10. 1 hit.
InterProiIPR011084. DRMBL.
IPR001279. Metallo-B-lactamas.
[Graphical view]
PfamiPF07522. DRMBL. 1 hit.
[Graphical view]
SUPFAMiSSF56281. SSF56281. 1 hit.

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8K4J0-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSSFQGQMAE YPTISIDRFD RENLKARAYF LSHCHKDHMK GLRAPSLKRR
60 70 80 90 100
LECSLKVFLY CSPVTKELLL TSPKYRFWEN RIITIEIETP TQISLVDEAS
110 120 130 140 150
GEKEEVVVTL LPAGHCPGSV MFLFQGSNGT VLYTGDFRLA KGEASRMELL
160 170 180 190 200
HSGGRVKDIQ SVYLDTTFCD PRFYQIPSRE QCLRGILELV RSWVTRSPHH
210 220 230 240 250
VVWLNCKAAY GYEYLFTNLS EELGVQVHVD KLDMFKNMPD ILHHLTTDRN
260 270 280 290 300
TQIHACRHPK AEECFQWNKL PCGITSQNKT ALHTISIKPS TMWFGERTRK
310 320 330 340 350
TNVIVRTGES SYRACFSFHS SFSEIKDFLS YICPVNVYPN VIPVGLTVDK
360 370 380 390 400
VMDVLKPLCR SPQSVEPKYK PLGKLKRART IHLDSEEDDD LFDDPLPTPL
410 420 430 440 450
RHKVPYQLTL QPELFSMKAL PLDQPELRQS PGGCKAESVW SPSLANFIDC
460 470 480 490 500
EESNSDSGEE LETPPPSLQG GLGPSTLVQQ NADPDVDIPQ WEVFFKRRDE
510 520 530 540 550
ITGECLEHLP SSIETGGSQS PKLCSDSPKL CSDSPKLCSD SDGDSTHISS
560 570 580 590 600
QNSSQSTHIT DQGSQGWDSQ CDTVLLSSQE KSGGDSTSLN KGAYKPKLKE
610 620 630 640 650
SISASQIEQD ALCPQDTHCD LKSRAEVNGA PCLVELDTLS GRKSPPEKTL
660 670 680 690 700
LSSTRADSQS SSDFEIPSTP EAELPTPEHL QCLYRKLATG QSIVVEKRKC

SLLDS
Length:705
Mass (Da):78,834
Last modified:July 19, 2005 - v2
Checksum:i7BEA71D61F0B5063
GO
Isoform 2 (identifier: Q8K4J0-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     503-603: GECLEHLPSS...YKPKLKESIS → VDTMIRTPRP...TFKKKTKTLL
     604-705: Missing.

Note: May be due to an intron retention. No experimental confirmation available.
Show »
Length:603
Mass (Da):68,796
Checksum:iBB2F533224E338EC
GO
Isoform 3 (identifier: Q8K4J0-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-130: Missing.
     131-154: VLYTGDFRLAKGEASRMELLHSGG → MRLRVRRLQTGKRRSFQNGASALW
     503-603: GECLEHLPSS...YKPKLKESIS → VDTMIRTPRP...TFKKKTKTLL
     604-705: Missing.

Note: No experimental confirmation available.
Show »
Length:473
Mass (Da):54,281
Checksum:i137F13282EAE9B98
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti103 – 1031K → Q in AAM89119 (PubMed:15699179).Curated
Sequence conflicti105 – 1051E → Q in AAM89119 (PubMed:15699179).Curated
Sequence conflicti107 – 1071V → G in AAM89119 (PubMed:15699179).Curated
Sequence conflicti113 – 1131A → P in AAM89119 (PubMed:15699179).Curated
Sequence conflicti118 – 1181G → R in AAM89119 (PubMed:15699179).Curated
Sequence conflicti121 – 1211M → V in AAM89119 (PubMed:15699179).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 130130Missing in isoform 3. 1 PublicationVSP_014893Add
BLAST
Alternative sequencei131 – 15424VLYTG…LHSGG → MRLRVRRLQTGKRRSFQNGA SALW in isoform 3. 1 PublicationVSP_014894Add
BLAST
Alternative sequencei503 – 603101GECLE…KESIS → VDTMIRTPRPRKMKGCGQWS LKMLLQNLEIQEEKHIFENR GWKMAGQVKGSCGLLEGQSS LPTFKLATSLASNSSFWPPW HLHSHAHTQIFTFKKKTKTL L in isoform 2 and isoform 3. 1 PublicationVSP_014895Add
BLAST
Alternative sequencei604 – 705102Missing in isoform 2 and isoform 3. 1 PublicationVSP_014896Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF387731 mRNA. Translation: AAM89119.1.
AK037126 mRNA. Translation: BAC29713.1.
AK052369 mRNA. Translation: BAC34960.1.
AK088810 mRNA. Translation: BAC40586.1.
AL732620 Genomic DNA. Translation: CAM16038.1.
AL732620 Genomic DNA. Translation: CAM16039.1.
AL732620 Genomic DNA. Translation: CAM16040.1.
CCDSiCCDS15649.1. [Q8K4J0-2]
CCDS15650.1. [Q8K4J0-1]
CCDS79728.1. [Q8K4J0-3]
RefSeqiNP_001103684.1. NM_001110214.1.
NP_001289603.1. NM_001302674.1. [Q8K4J0-3]
NP_001289613.1. NM_001302684.1.
NP_666226.2. NM_146114.3. [Q8K4J0-1]
NP_783614.1. NM_175683.4. [Q8K4J0-2]
XP_011237274.1. XM_011238972.1. [Q8K4J0-3]
UniGeneiMm.23483.
Mm.442710.

Genome annotation databases

EnsembliENSMUST00000061852; ENSMUSP00000054300; ENSMUSG00000026648. [Q8K4J0-2]
ENSMUST00000102988; ENSMUSP00000100053; ENSMUSG00000026648. [Q8K4J0-1]
ENSMUST00000115066; ENSMUSP00000110718; ENSMUSG00000026648. [Q8K4J0-3]
GeneIDi227525.
KEGGimmu:227525.
UCSCiuc008idz.2. mouse. [Q8K4J0-1]
uc008iea.3. mouse. [Q8K4J0-2]
uc008ieb.3. mouse. [Q8K4J0-3]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF387731 mRNA. Translation: AAM89119.1.
AK037126 mRNA. Translation: BAC29713.1.
AK052369 mRNA. Translation: BAC34960.1.
AK088810 mRNA. Translation: BAC40586.1.
AL732620 Genomic DNA. Translation: CAM16038.1.
AL732620 Genomic DNA. Translation: CAM16039.1.
AL732620 Genomic DNA. Translation: CAM16040.1.
CCDSiCCDS15649.1. [Q8K4J0-2]
CCDS15650.1. [Q8K4J0-1]
CCDS79728.1. [Q8K4J0-3]
RefSeqiNP_001103684.1. NM_001110214.1.
NP_001289603.1. NM_001302674.1. [Q8K4J0-3]
NP_001289613.1. NM_001302684.1.
NP_666226.2. NM_146114.3. [Q8K4J0-1]
NP_783614.1. NM_175683.4. [Q8K4J0-2]
XP_011237274.1. XM_011238972.1. [Q8K4J0-3]
UniGeneiMm.23483.
Mm.442710.

3D structure databases

ProteinModelPortaliQ8K4J0.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000100053.

PTM databases

iPTMnetiQ8K4J0.
PhosphoSiteiQ8K4J0.

Proteomic databases

PaxDbiQ8K4J0.
PRIDEiQ8K4J0.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000061852; ENSMUSP00000054300; ENSMUSG00000026648. [Q8K4J0-2]
ENSMUST00000102988; ENSMUSP00000100053; ENSMUSG00000026648. [Q8K4J0-1]
ENSMUST00000115066; ENSMUSP00000110718; ENSMUSG00000026648. [Q8K4J0-3]
GeneIDi227525.
KEGGimmu:227525.
UCSCiuc008idz.2. mouse. [Q8K4J0-1]
uc008iea.3. mouse. [Q8K4J0-2]
uc008ieb.3. mouse. [Q8K4J0-3]

Organism-specific databases

CTDi64421.
MGIiMGI:2441769. Dclre1c.

Phylogenomic databases

eggNOGiKOG1361. Eukaryota.
COG1236. LUCA.
GeneTreeiENSGT00530000063183.
HOGENOMiHOG000231568.
HOVERGENiHBG081421.
InParanoidiQ8K4J0.
KOiK10887.
OrthoDBiEOG70CR8F.
PhylomeDBiQ8K4J0.
TreeFamiTF329572.

Enzyme and pathway databases

ReactomeiR-MMU-5693571. Nonhomologous End-Joining (NHEJ).

Miscellaneous databases

PROiQ8K4J0.
SOURCEiSearch...

Gene expression databases

BgeeiQ8K4J0.
ExpressionAtlasiQ8K4J0. baseline and differential.
GenevisibleiQ8K4J0. MM.

Family and domain databases

Gene3Di3.60.15.10. 1 hit.
InterProiIPR011084. DRMBL.
IPR001279. Metallo-B-lactamas.
[Graphical view]
PfamiPF07522. DRMBL. 1 hit.
[Graphical view]
SUPFAMiSSF56281. SSF56281. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Targeted disruption of the Artemis murine counterpart results in SCID and defective V(D)J recombination that is partially corrected with bone marrow transplantation."
    Li L., Salido E., Zhou Y., Bhattacharyya S., Yannone S.M., Dunn E., Meneses J., Feeney A.J., Cowan M.J.
    J. Immunol. 174:2420-2428(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION.
  2. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 2 AND 3).
    Strain: NOD.
    Tissue: Thymus.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  4. "Leaky Scid phenotype associated with defective V(D)J coding end processing in Artemis-deficient mice."
    Rooney S., Sekiguchi J., Zhu C., Cheng H.-L., Manis J., Whitlow S., DeVido J., Foy D., Chaudhuri J., Lombard D., Alt F.W.
    Mol. Cell 10:1379-1390(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  5. "Metallo-beta-lactamase fold within nucleic acids processing enzymes: the beta-CASP family."
    Callebaut I., Moshous D., Mornon J.-P., de Villartay J.-P.
    Nucleic Acids Res. 30:3592-3601(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: DNA REPAIR METALLO-BETA-LACTAMASE FAMILY.
  6. "Defective DNA repair and increased genomic instability in Artemis-deficient murine cells."
    Rooney S., Alt F.W., Lombard D., Whitlow S., Eckersdorff M., Fleming J., Fugmann S., Ferguson D.O., Schatz D.G., Sekiguchi J.
    J. Exp. Med. 197:553-565(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  7. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-380 AND SER-385, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Lung and Spleen.

Entry informationi

Entry nameiDCR1C_MOUSE
AccessioniPrimary (citable) accession number: Q8K4J0
Secondary accession number(s): A2AJG6
, A2AJG7, A2AJG8, Q8BG72, Q8BTT1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 19, 2005
Last sequence update: July 19, 2005
Last modified: June 8, 2016
This is version 100 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.