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Protein

Rho guanine nucleotide exchange factor 6

Gene

Arhgef6

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Acts as a RAC1 guanine nucleotide exchange factor (GEF).By similarity

GO - Molecular functioni

GO - Biological processi

  • intracellular signal transduction Source: InterPro
  • lamellipodium assembly Source: UniProtKB
  • regulation of Rho protein signal transduction Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Guanine-nucleotide releasing factor

Names & Taxonomyi

Protein namesi
Recommended name:
Rho guanine nucleotide exchange factor 6
Alternative name(s):
Alpha-PIX
Rac/Cdc42 guanine nucleotide exchange factor 6
Gene namesi
Name:Arhgef6
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:1920591. Arhgef6.

Subcellular locationi

GO - Cellular componenti

  • cell-cell junction Source: MGI
  • intracellular Source: GOC
  • lamellipodium Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cell projection

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000809181 – 771Rho guanine nucleotide exchange factor 6Add BLAST771

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei126PhosphoserineBy similarity1
Modified residuei133PhosphothreonineBy similarity1
Modified residuei224PhosphoserineBy similarity1
Modified residuei487PhosphoserineCombined sources1
Modified residuei639PhosphoserineCombined sources1
Modified residuei679PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ8K4I3.
MaxQBiQ8K4I3.
PaxDbiQ8K4I3.
PRIDEiQ8K4I3.

PTM databases

iPTMnetiQ8K4I3.
PhosphoSitePlusiQ8K4I3.

Expressioni

Tissue specificityi

Detected in adult heart, spleen, lung, skeletal muscle, kidney and testis. Detected throughout embryogenesis.1 Publication

Gene expression databases

BgeeiENSMUSG00000031133.

Interactioni

Subunit structurei

Interacts with PAK kinases through the SH3 domain. Interacts with GIT1. Component of cytoplasmic complexes, which also contain PXN, GIT1 and PAK1. Interacts with BIN2. Identified in a complex with BIN2 and GIT2 (By similarity). Interacts with PARVB.By similarity1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
PARVBQ9HBI13EBI-6272809,EBI-1047679From a different organism.

Protein-protein interaction databases

BioGridi215941. 7 interactors.
IntActiQ8K4I3. 4 interactors.
MINTiMINT-4117855.
STRINGi10090.ENSMUSP00000033468.

Structurei

Secondary structure

1771
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Turni430 – 432Combined sources3
Turni444 – 446Combined sources3
Beta strandi452 – 457Combined sources6
Beta strandi460 – 463Combined sources4
Beta strandi467 – 472Combined sources6
Beta strandi477 – 481Combined sources5
Beta strandi483 – 487Combined sources5
Beta strandi490 – 495Combined sources6
Turni497 – 499Combined sources3
Beta strandi507 – 511Combined sources5
Beta strandi515 – 518Combined sources4
Beta strandi520 – 522Combined sources3
Beta strandi524 – 527Combined sources4
Helixi533 – 546Combined sources14

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1V61NMR-A429-547[»]
ProteinModelPortaliQ8K4I3.
SMRiQ8K4I3.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ8K4I3.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini1 – 110CHPROSITE-ProRule annotationAdd BLAST110
Domaini159 – 218SH3PROSITE-ProRule annotationAdd BLAST60
Domaini240 – 420DHPROSITE-ProRule annotationAdd BLAST181
Domaini442 – 547PHPROSITE-ProRule annotationAdd BLAST106

Sequence similaritiesi

Contains 1 CH (calponin-homology) domain.PROSITE-ProRule annotation
Contains 1 DH (DBL-homology) domain.PROSITE-ProRule annotation
Contains 1 PH domain.PROSITE-ProRule annotation
Contains 1 SH3 domain.PROSITE-ProRule annotation

Keywords - Domaini

SH3 domain

Phylogenomic databases

eggNOGiKOG2070. Eukaryota.
ENOG410XNNP. LUCA.
HOGENOMiHOG000231381.
HOVERGENiHBG050569.
InParanoidiQ8K4I3.
KOiK05729.
PhylomeDBiQ8K4I3.

Family and domain databases

CDDicd00014. CH. 1 hit.
Gene3Di1.10.418.10. 1 hit.
1.20.900.10. 1 hit.
2.30.29.30. 1 hit.
InterProiIPR001715. CH-domain.
IPR000219. DH-domain.
IPR001331. GDS_CDC24_CS.
IPR032409. GEF_CC.
IPR011993. PH_dom-like.
IPR001849. PH_domain.
IPR001452. SH3_domain.
IPR003096. SM22_calponin.
[Graphical view]
PfamiPF16523. betaPIX_CC. 1 hit.
PF00307. CH. 1 hit.
PF00169. PH. 1 hit.
PF00621. RhoGEF. 1 hit.
PF14604. SH3_9. 1 hit.
[Graphical view]
PRINTSiPR00452. SH3DOMAIN.
PR00888. SM22CALPONIN.
SMARTiSM00033. CH. 1 hit.
SM00233. PH. 1 hit.
SM00325. RhoGEF. 1 hit.
SM00326. SH3. 1 hit.
[Graphical view]
SUPFAMiSSF47576. SSF47576. 1 hit.
SSF48065. SSF48065. 1 hit.
SSF50044. SSF50044. 1 hit.
SSF50729. SSF50729. 1 hit.
PROSITEiPS50021. CH. 1 hit.
PS00741. DH_1. 1 hit.
PS50010. DH_2. 1 hit.
PS50003. PH_DOMAIN. 1 hit.
PS50002. SH3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8K4I3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNPEERLVTW LISLGVLESP KKTVCDPEEF LKSSLKNGVV LCKLINRLLP
60 70 80 90 100
GSVEKYCLEP QTEADCIDNI NDFLKGCATL QVEVFEPDDL YSGANFSKVL
110 120 130 140 150
NTLLAVNKAT EDQLSERPCG RSSSLSAATS SQTNPQVAVP STAPEQHSEE
160 170 180 190 200
KAEMTENGSH QLIVKARFNF KQTNEDELSV CKGDIIYVTR VEEGGWWEGT
210 220 230 240 250
LNGRTGWFPS NYVREIKPSE RPLSPKAIKG FDTAPLTKNY YTVVLQNILD
260 270 280 290 300
TEKEYAKELQ SLLVTYLRPL QSNNNLSTVE FTCLLGNFEE VCTFQQTLCQ
310 320 330 340 350
ALEECSKFPE NQHKVGGCLL NLMPHFKSMY LAYCANHPSA VNVLTQHSDD
360 370 380 390 400
LERFMENQGA SSPGILILTT SLSKPFMRLE KYVTLLQELE RHMEDTHPDH
410 420 430 440 450
QDILKAIIAF KTLMGQCQDL RKRKQLELQI LSEPIQAWEG DDIKTLGNVI
460 470 480 490 500
FMSQVVMQHG ACEEKEERYF LLFSSVLIML SASPRMSGFM YQGKIPIAGM
510 520 530 540 550
VVNRLDEIEG SDCMFEITGS TVERIVVHCN NNQDFQEWME QLNRLTKGPT
560 570 580 590 600
SCGSLSKTSS SSCSTHSSFS STGQPRGPLE PPQIIKPWSL SCLRPAPPLR
610 620 630 640 650
PSAALGYKER MSYILKESSK SPKTMKKFLH KRKTERKASE EEYVIRKSTA
660 670 680 690 700
ALEEDAQILK VIEAYCTSAS FQQGTRKDSV PQVLLPEEEK LIIEETRSNG
710 720 730 740 750
QTIIEEKSLV DTVYALKDEV KELKQENKKM KQCLEEELKS RKDLEKLVRK
760 770
LLKQTDECIR SESSSKTSIL Q
Length:771
Mass (Da):87,051
Last modified:October 1, 2002 - v1
Checksum:i3A408B7D0D6FCEAE
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF393831 mRNA. Translation: AAM94903.1.
AK040468 mRNA. Translation: BAC30599.1.
RefSeqiNP_690014.2. NM_152801.2.
UniGeneiMm.261443.

Genome annotation databases

GeneIDi73341.
KEGGimmu:73341.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF393831 mRNA. Translation: AAM94903.1.
AK040468 mRNA. Translation: BAC30599.1.
RefSeqiNP_690014.2. NM_152801.2.
UniGeneiMm.261443.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1V61NMR-A429-547[»]
ProteinModelPortaliQ8K4I3.
SMRiQ8K4I3.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi215941. 7 interactors.
IntActiQ8K4I3. 4 interactors.
MINTiMINT-4117855.
STRINGi10090.ENSMUSP00000033468.

PTM databases

iPTMnetiQ8K4I3.
PhosphoSitePlusiQ8K4I3.

Proteomic databases

EPDiQ8K4I3.
MaxQBiQ8K4I3.
PaxDbiQ8K4I3.
PRIDEiQ8K4I3.

Protocols and materials databases

DNASUi73341.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi73341.
KEGGimmu:73341.

Organism-specific databases

CTDi9459.
MGIiMGI:1920591. Arhgef6.

Phylogenomic databases

eggNOGiKOG2070. Eukaryota.
ENOG410XNNP. LUCA.
HOGENOMiHOG000231381.
HOVERGENiHBG050569.
InParanoidiQ8K4I3.
KOiK05729.
PhylomeDBiQ8K4I3.

Miscellaneous databases

EvolutionaryTraceiQ8K4I3.
PROiQ8K4I3.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000031133.

Family and domain databases

CDDicd00014. CH. 1 hit.
Gene3Di1.10.418.10. 1 hit.
1.20.900.10. 1 hit.
2.30.29.30. 1 hit.
InterProiIPR001715. CH-domain.
IPR000219. DH-domain.
IPR001331. GDS_CDC24_CS.
IPR032409. GEF_CC.
IPR011993. PH_dom-like.
IPR001849. PH_domain.
IPR001452. SH3_domain.
IPR003096. SM22_calponin.
[Graphical view]
PfamiPF16523. betaPIX_CC. 1 hit.
PF00307. CH. 1 hit.
PF00169. PH. 1 hit.
PF00621. RhoGEF. 1 hit.
PF14604. SH3_9. 1 hit.
[Graphical view]
PRINTSiPR00452. SH3DOMAIN.
PR00888. SM22CALPONIN.
SMARTiSM00033. CH. 1 hit.
SM00233. PH. 1 hit.
SM00325. RhoGEF. 1 hit.
SM00326. SH3. 1 hit.
[Graphical view]
SUPFAMiSSF47576. SSF47576. 1 hit.
SSF48065. SSF48065. 1 hit.
SSF50044. SSF50044. 1 hit.
SSF50729. SSF50729. 1 hit.
PROSITEiPS50021. CH. 1 hit.
PS00741. DH_1. 1 hit.
PS50010. DH_2. 1 hit.
PS50003. PH_DOMAIN. 1 hit.
PS50002. SH3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiARHG6_MOUSE
AccessioniPrimary (citable) accession number: Q8K4I3
Secondary accession number(s): Q8C9V4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 27, 2005
Last sequence update: October 1, 2002
Last modified: November 30, 2016
This is version 117 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.