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Q8K4H4

- APTX_RAT

UniProt

Q8K4H4 - APTX_RAT

Protein

Aprataxin

Gene

Aptx

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 85 (01 Oct 2014)
      Sequence version 1 (01 Oct 2002)
      Previous versions | rss
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    Functioni

    DNA-binding protein involved in single-strand DNA break repair, double-strand DNA break repair and base excision repair. Resolves abortive DNA ligation intermediates formed either at base excision sites, or when DNA ligases attempt to repair non-ligatable breaks induced by reactive oxygen species. Catalyzes the release of adenylate groups covalently linked to 5'-phosphate termini, resulting in the production of 5'-phosphate termini that can be efficiently rejoined. Also able to hydrolyze adenosine 5'-monophosphoramidate (AMP-NH2) and diadenosine tetraphosphate (AppppA), but with lower catalytic activity By similarity.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei247 – 2471Tele-AMP-histidine intermediateBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri304 – 32623C2H2-typePROSITE-ProRule annotationAdd
    BLAST

    GO - Molecular functioni

    1. chromatin binding Source: Ensembl
    2. damaged DNA binding Source: Ensembl
    3. DNA 5'-adenosine monophosphate hydrolase activity Source: Ensembl
    4. double-stranded DNA binding Source: Ensembl
    5. double-stranded RNA binding Source: Ensembl
    6. metal ion binding Source: UniProtKB-KW
    7. phosphoglycolate phosphatase activity Source: Ensembl
    8. polynucleotide 3'-phosphatase activity Source: Ensembl

    GO - Biological processi

    1. DNA ligation Source: Ensembl
    2. double-strand break repair Source: Ensembl
    3. regulation of protein stability Source: Ensembl
    4. response to hydrogen peroxide Source: Ensembl
    5. single strand break repair Source: Ensembl

    Keywords - Molecular functioni

    Hydrolase

    Keywords - Biological processi

    DNA damage, DNA repair

    Keywords - Ligandi

    DNA-binding, Metal-binding, Zinc

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Aprataxin (EC:3.-.-.-)
    Alternative name(s):
    Forkhead-associated domain histidine triad-like protein
    Short name:
    FHA-HIT
    Gene namesi
    Name:Aptx
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Chromosome 5

    Organism-specific databases

    RGDi628740. Aptx.

    Subcellular locationi

    Nucleusnucleoplasm By similarity. Nucleusnucleolus By similarity
    Note: Upon genotoxic stress, colocalizes with XRCC1 at sites of DNA damage. Colocalizes with MDC1 at sites of DNA double-strand breaks. Interaction with NCL is required for nucleolar localization By similarity.By similarity

    GO - Cellular componenti

    1. nuclear chromatin Source: Ensembl
    2. nucleolus Source: UniProtKB-SubCell
    3. nucleoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 329329AprataxinPRO_0000109842Add
    BLAST

    Proteomic databases

    PRIDEiQ8K4H4.

    Expressioni

    Gene expression databases

    GenevestigatoriQ8K4H4.

    Interactioni

    Subunit structurei

    Interacts with single-strand break repair proteins XRCC1, XRCC4, ADPRT and p53/TP53. Interacts with NCL. Interacts (via FHA-like domain) with MDC1 (phosphorylated) By similarity.By similarity

    Protein-protein interaction databases

    STRINGi10116.ENSRNOP00000009176.

    Structurei

    3D structure databases

    ProteinModelPortaliQ8K4H4.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini23 – 7250FHA-likeAdd
    BLAST
    Domaini155 – 260106HITPROSITE-ProRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni1 – 9595Interactions with ADPRT and NCLBy similarityAdd
    BLAST

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi111 – 1155Nuclear localization signalBy similarity
    Motifi245 – 2495Histidine triad motif

    Domaini

    The histidine triad, also called HIT motif, forms part of the binding loop for the alpha-phosphate of purine mononucleotide.By similarity
    The FHA-like domain mediates interaction with NCL; XRCC1 and XRCC4.By similarity
    The HIT domain is required for enzymatic activity.By similarity
    The C2H2-type zinc finger mediates DNA-binding.By similarity

    Sequence similaritiesi

    Contains 1 C2H2-type zinc finger.PROSITE-ProRule annotation
    Contains 1 FHA-like domain.Curated
    Contains 1 HIT domain.PROSITE-ProRule annotation

    Zinc finger

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri304 – 32623C2H2-typePROSITE-ProRule annotationAdd
    BLAST

    Keywords - Domaini

    Zinc-finger

    Phylogenomic databases

    eggNOGiNOG278510.
    GeneTreeiENSGT00570000079163.
    HOGENOMiHOG000248858.
    HOVERGENiHBG050555.
    InParanoidiQ8K4H4.
    KOiK10863.
    OMAiPGQVLHM.
    OrthoDBiEOG786H3J.
    PhylomeDBiQ8K4H4.

    Family and domain databases

    Gene3Di2.60.200.20. 2 hits.
    3.30.428.10. 1 hit.
    InterProiIPR026963. Aprataxin.
    IPR000253. FHA_dom.
    IPR019808. Histidine_triad_CS.
    IPR001310. Histidine_triad_HIT.
    IPR011146. HIT-like.
    IPR008984. SMAD_FHA_domain.
    IPR007087. Znf_C2H2.
    IPR015880. Znf_C2H2-like.
    [Graphical view]
    PANTHERiPTHR12486. PTHR12486. 1 hit.
    PTHR12486:SF4. PTHR12486:SF4. 1 hit.
    SMARTiSM00355. ZnF_C2H2. 1 hit.
    [Graphical view]
    SUPFAMiSSF49879. SSF49879. 1 hit.
    SSF54197. SSF54197. 1 hit.
    PROSITEiPS00892. HIT_1. 1 hit.
    PS51084. HIT_2. 1 hit.
    PS00028. ZINC_FINGER_C2H2_1. 1 hit.
    PS50157. ZINC_FINGER_C2H2_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q8K4H4-1 [UniParc]FASTAAdd to Basket

    « Hide

    MRVCWLVRQD CWHQRIKLPH LEAVVIGRSP ETKITDKKCS RQQVQLKAEC    50
    NKRYVNVKQM GVNPTSIDEV VIGKDREMKL LPGQVLHMVN ELYPYMVEFE 100
    EVAESPNVTQ RKRKRPDCDS QEMEAEAGAS PSQCSVSPKT GKHGAAKEES 150
    LGHWSQGLKI SMKDPKMQVY KDDQVVVIKD KYPKARHHWL VLPWASISSL 200
    KVVTSEHLEL LKHMHAVGEK VIADFTGSSK LRFRLGYHAI PSMSHVHLHV 250
    ISQDFDSPCL KNKKHWNSFN TEYFLESQAV IKMVQEAGRV TVKDGTCELL 300
    KLPLRCHECQ QLLPSIPQLK EHLRKHWGG 329
    Length:329
    Mass (Da):37,720
    Last modified:October 1, 2002 - v1
    Checksum:i636B827F661B14FD
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF398235 mRNA. Translation: AAM90583.1.
    BC078716 mRNA. Translation: AAH78716.1.
    RefSeqiNP_683687.1. NM_148889.2.
    XP_006238089.1. XM_006238027.1.
    XP_006238090.1. XM_006238028.1.
    UniGeneiRn.50459.

    Genome annotation databases

    EnsembliENSRNOT00000046463; ENSRNOP00000046400; ENSRNOG00000006582.
    GeneIDi259271.
    KEGGirno:259271.
    UCSCiRGD:628740. rat.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF398235 mRNA. Translation: AAM90583.1 .
    BC078716 mRNA. Translation: AAH78716.1 .
    RefSeqi NP_683687.1. NM_148889.2.
    XP_006238089.1. XM_006238027.1.
    XP_006238090.1. XM_006238028.1.
    UniGenei Rn.50459.

    3D structure databases

    ProteinModelPortali Q8K4H4.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 10116.ENSRNOP00000009176.

    Proteomic databases

    PRIDEi Q8K4H4.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSRNOT00000046463 ; ENSRNOP00000046400 ; ENSRNOG00000006582 .
    GeneIDi 259271.
    KEGGi rno:259271.
    UCSCi RGD:628740. rat.

    Organism-specific databases

    CTDi 54840.
    RGDi 628740. Aptx.

    Phylogenomic databases

    eggNOGi NOG278510.
    GeneTreei ENSGT00570000079163.
    HOGENOMi HOG000248858.
    HOVERGENi HBG050555.
    InParanoidi Q8K4H4.
    KOi K10863.
    OMAi PGQVLHM.
    OrthoDBi EOG786H3J.
    PhylomeDBi Q8K4H4.

    Miscellaneous databases

    NextBioi 624321.

    Gene expression databases

    Genevestigatori Q8K4H4.

    Family and domain databases

    Gene3Di 2.60.200.20. 2 hits.
    3.30.428.10. 1 hit.
    InterProi IPR026963. Aprataxin.
    IPR000253. FHA_dom.
    IPR019808. Histidine_triad_CS.
    IPR001310. Histidine_triad_HIT.
    IPR011146. HIT-like.
    IPR008984. SMAD_FHA_domain.
    IPR007087. Znf_C2H2.
    IPR015880. Znf_C2H2-like.
    [Graphical view ]
    PANTHERi PTHR12486. PTHR12486. 1 hit.
    PTHR12486:SF4. PTHR12486:SF4. 1 hit.
    SMARTi SM00355. ZnF_C2H2. 1 hit.
    [Graphical view ]
    SUPFAMi SSF49879. SSF49879. 1 hit.
    SSF54197. SSF54197. 1 hit.
    PROSITEi PS00892. HIT_1. 1 hit.
    PS51084. HIT_2. 1 hit.
    PS00028. ZINC_FINGER_C2H2_1. 1 hit.
    PS50157. ZINC_FINGER_C2H2_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Identification of FHA-HIT as a novel nuclear protein involved in cell-cycle regulation."
      Huang C.-H., Chen H., Peng J., Chen Y.
      Submitted (JUL-2001) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Testis.

    Entry informationi

    Entry nameiAPTX_RAT
    AccessioniPrimary (citable) accession number: Q8K4H4
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: June 7, 2004
    Last sequence update: October 1, 2002
    Last modified: October 1, 2014
    This is version 85 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3