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Protein

Kynurenine formamidase

Gene

Afmid

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the hydrolysis of N-formyl-L-kynurenine to L-kynurenine, the second step in the kynurenine pathway of tryptophan degradation. Kynurenine may be further oxidized to nicotinic acid, NAD(H) and NADP(H). Required for elimination of toxic metabolites.UniRule annotation1 Publication

Miscellaneous

The Afmid gene shares a bidirectional promoter region with Tk1 gene.

Catalytic activityi

N-formyl-L-kynurenine + H2O = formate + L-kynurenine.UniRule annotation

Kineticsi

  1. KM=0.18 mM for N-formyl-L-kynurenine1 Publication
  1. Vmax=42 µmol/min/mg enzyme1 Publication

Pathwayi: L-tryptophan degradation via kynurenine pathway

This protein is involved in step 2 of the subpathway that synthesizes L-kynurenine from L-tryptophan.UniRule annotation
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Tryptophan 2,3-dioxygenase (Tdo2), Indoleamine 2,3-dioxygenase 2 (Ido2), Tryptophan 2,3-dioxygenase (Tdo2), Tryptophan 2,3-dioxygenase (Tdo2)
  2. Kynurenine formamidase (Afmid)
This subpathway is part of the pathway L-tryptophan degradation via kynurenine pathway, which is itself part of Amino-acid degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-kynurenine from L-tryptophan, the pathway L-tryptophan degradation via kynurenine pathway and in Amino-acid degradation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei162NucleophileCurated1
Active sitei247Curated1
Active sitei279Curated1

GO - Molecular functioni

GO - Biological processi

  • tryptophan catabolic process to kynurenine Source: MGI

Keywordsi

Molecular functionHydrolase
Biological processTryptophan catabolism

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-15003
BRENDAi3.5.1.9 3474
SABIO-RKQ8K4H1
UniPathwayiUPA00333; UER00454

Protein family/group databases

ESTHERimouse-KFA Kynurenine-formamidase
MEROPSiS09.977

Names & Taxonomyi

Protein namesi
Recommended name:
Kynurenine formamidaseUniRule annotation (EC:3.5.1.9UniRule annotation)
Short name:
KFAUniRule annotation
Short name:
KFaseUniRule annotation
Alternative name(s):
ArylformamidaseUniRule annotation
N-formylkynurenine formamidaseUniRule annotation
Short name:
FKFUniRule annotation
Gene namesi
Name:Afmid
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 11

Organism-specific databases

MGIiMGI:2448704 Afmid

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Disruption phenotypei

Mice display sclerosis of kidney glomeruli, possibly due to failures in the elimination of toxic metabolites.1 Publication

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi162S → A: Loss of enzyme activity. 1 Publication1
Mutagenesisi247D → A: Loss of enzyme activity. 1 Publication1
Mutagenesisi279H → A: Loss of enzyme activity. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002483091 – 305Kynurenine formamidaseAdd BLAST305

Post-translational modificationi

The N-terminus is blocked.

Proteomic databases

PaxDbiQ8K4H1
PRIDEiQ8K4H1

PTM databases

iPTMnetiQ8K4H1
PhosphoSitePlusiQ8K4H1

Expressioni

Tissue specificityi

Highly expressed in liver. Expressed in kidney. Weakly or not expressed in other tissues.1 Publication

Inductioni

Down-regulated upon IL2-mediated activation. Transcriptional activation correlates with reduced histone acetylation.1 Publication

Gene expression databases

BgeeiENSMUSG00000017718
CleanExiMM_AFMID
ExpressionAtlasiQ8K4H1 baseline and differential
GenevisibleiQ8K4H1 MM

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

IntActiQ8K4H1, 2 interactors
MINTiQ8K4H1
STRINGi10090.ENSMUSP00000073102

Structurei

3D structure databases

ProteinModelPortaliQ8K4H1
SMRiQ8K4H1
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi93 – 97HGGXW5

Domaini

The main chain amide nitrogen atoms of the second glycine and its adjacent residue in the HGGXW motif define the oxyanion hole, and stabilize the oxyanion that forms during the nucleophilic attack by the catalytic serine during substrate cleavage.UniRule annotation

Sequence similaritiesi

Belongs to the kynurenine formamidase family.UniRule annotation

Phylogenomic databases

eggNOGiKOG4627 Eukaryota
COG0657 LUCA
GeneTreeiENSGT00390000011093
HOGENOMiHOG000260457
HOVERGENiHBG100436
InParanoidiQ8K4H1
KOiK01432
PhylomeDBiQ8K4H1
TreeFamiTF315112

Family and domain databases

Gene3Di3.40.50.1820, 1 hit
HAMAPiMF_03014 KFase, 1 hit
InterProiView protein in InterPro
IPR029058 AB_hydrolase
IPR013094 AB_hydrolase_3
IPR027519 KFase_ver/fungi-typ
PfamiView protein in Pfam
PF07859 Abhydrolase_3, 1 hit
SUPFAMiSSF53474 SSF53474, 1 hit

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8K4H1-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAFPSLSAGQ NPWRNLSSEE LEKQYSPSRW VIHTKPEEVV GNFVQIGSQA
60 70 80 90 100
TQKARATRRN QLDVPYGDGE GEKLDIYFPD EDSKAFPLFL FLHGGYWQSG
110 120 130 140 150
SKDDSAFMVN PLTAQGIVVV IVAYDIAPKG TLDQMVDQVT RSVVFLQRRY
160 170 180 190 200
PSNEGIYLCG HSAGAHLAAM VLLARWTKHG VTPNLQGFLL VSGIYDLEPL
210 220 230 240 250
IATSQNDPLR MTLEDAQRNS PQRHLDVVPA QPVAPACPVL VLVGQHDSPE
260 270 280 290 300
FHRQSKEFYE TLLRVGWKAS FQQLRGVDHF DIIENLTRED DVLTQIILKT

VFQKL
Length:305
Mass (Da):34,229
Last modified:October 1, 2002 - v1
Checksum:i898000A577132A52
GO
Isoform 2 (identifier: Q8K4H1-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     296-305: IILKTVFQKL → VGLTPTTVFL...ITLAGLGLKI

Note: No experimental confirmation available.
Show »
Length:371
Mass (Da):41,574
Checksum:i0F20521893042E06
GO

Sequence cautioni

The sequence AAH24452 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti33H → R in AAH43309 (PubMed:15489334).Curated1
Sequence conflicti33H → R in AAH24452 (PubMed:15489334).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_020237296 – 305IILKTVFQKL → VGLTPTTVFLNGQRGSHAHT WAWPGRQEQRERLAVPRWKS QSRLHRRACGECLLFIIVVA ETMSHCITLAGLGLKI in isoform 2. 1 Publication10

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF399717 mRNA Translation: AAM62284.1
AY099479 mRNA Translation: AAM44406.1
AL591433 Genomic DNA No translation available.
BC024452 mRNA Translation: AAH24452.1 Different initiation.
BC043309 mRNA Translation: AAH43309.1
BC066780 mRNA Translation: AAH66780.1
CCDSiCCDS25693.1 [Q8K4H1-1]
RefSeqiNP_082103.1, NM_027827.3 [Q8K4H1-1]
XP_006534309.1, XM_006534246.1 [Q8K4H1-2]
UniGeneiMm.273958

Genome annotation databases

EnsembliENSMUST00000073388; ENSMUSP00000073102; ENSMUSG00000017718 [Q8K4H1-1]
GeneIDi71562
KEGGimmu:71562
UCSCiuc007moa.2 mouse [Q8K4H1-2]
uc007mob.2 mouse [Q8K4H1-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiKFA_MOUSE
AccessioniPrimary (citable) accession number: Q8K4H1
Secondary accession number(s): B1AQK9, Q80XP3, Q8R1K6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 5, 2006
Last sequence update: October 1, 2002
Last modified: May 23, 2018
This is version 117 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome
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Main funding by: National Institutes of Health