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Protein

Podocin

Gene

Nphs2

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Plays a role in the regulation of glomerular permeability, acting probably as a linker between the plasma membrane and the cytoskeleton.By similarity

GO - Biological processi

Complete GO annotation...

Enzyme and pathway databases

ReactomeiR-RNO-373753. Nephrin interactions.

Names & Taxonomyi

Protein namesi
Recommended name:
Podocin
Gene namesi
Name:Nphs2
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 13

Organism-specific databases

RGDi620461. Nphs2.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 102102CytoplasmicSequence analysisAdd
BLAST
Transmembranei103 – 12321HelicalSequence analysisAdd
BLAST
Topological domaini124 – 383260ExtracellularSequence analysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 383383PodocinPRO_0000094037Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Lipidationi101 – 1011S-palmitoyl cysteineBy similarity

Keywords - PTMi

Lipoprotein, Palmitate

Proteomic databases

PaxDbiQ8K4G9.

Interactioni

Subunit structurei

Interacts with nephrin/NPHS1, KIRRL1 and CD2AP (By similarity). Interacts with DDN.By similarity1 Publication

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000005425.

Structurei

3D structure databases

ProteinModelPortaliQ8K4G9.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the band 7/mec-2 family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG2621. Eukaryota.
COG0330. LUCA.
GeneTreeiENSGT00550000074454.
HOGENOMiHOG000217040.
HOVERGENiHBG004815.
InParanoidiQ8K4G9.
KOiK18268.
OMAiWFCIKVV.
OrthoDBiEOG78D7KJ.
PhylomeDBiQ8K4G9.
TreeFamiTF105750.

Family and domain databases

InterProiIPR001107. Band_7.
IPR028509. Podocin.
IPR001972. Stomatin_fam.
[Graphical view]
PANTHERiPTHR10264. PTHR10264. 1 hit.
PTHR10264:SF23. PTHR10264:SF23. 1 hit.
PfamiPF01145. Band_7. 1 hit.
[Graphical view]
PRINTSiPR00721. STOMATIN.
SMARTiSM00244. PHB. 1 hit.
[Graphical view]
SUPFAMiSSF117892. SSF117892. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8K4G9-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MDSRARSSSR KTHGRGSRSS SRDDKKSKAG RGNRGRARPD AGAERQSAGR
60 70 80 90 100
TGTREEHRAP AATVVNVDEV RSPGEEGTEV VALLESERPE EGIKPSGLGA
110 120 130 140 150
CEWLLVLSSL IFIIVTFPFS IWFCIKVVQE YERVIIFRLG HLLPGRAKGP
160 170 180 190 200
GLFFFLPCLD TYHKVDLRLQ TLEIPFHEVV TKDMFIMEID AVCYYRMENA
210 220 230 240 250
SLLLSSLAHV SKAIQFLVQT TMKRLLAHRS LTEILLERKS IAQDVKVALD
260 270 280 290 300
SVTCVWGIKV ERTEIKDVRL PAGLQHSLAV EAEAQRQAKV RVIAAEGEKA
310 320 330 340 350
ASESLRMAAE ILSGTPAAVQ LRYLHTLQSL STDKPSTVVL PLPFDMLNLL
360 370 380
SSPSNRAQGS INYPSSPKPV EPLNPKRKDS PML
Length:383
Mass (Da):42,387
Last modified:April 23, 2003 - v2
Checksum:i4B4D577A810EC21D
GO
Isoform 2 (identifier: Q8K4G9-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     248-292: Missing.

Show »
Length:338
Mass (Da):37,433
Checksum:i6033EB8359562DE4
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti43 – 431A → V in AAK71880 (PubMed:12506137).Curated
Sequence conflicti51 – 511T → M in AAK71880 (PubMed:12506137).Curated
Sequence conflicti78 – 792TE → RK in AAK71880 (PubMed:12506137).Curated
Sequence conflicti177 – 1771H → L in AAM90639 (Ref. 4) Curated
Sequence conflicti350 – 3501L → F in AAK71880 (PubMed:12506137).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei248 – 29245Missing in isoform 2. 1 PublicationVSP_007234Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB091379 mRNA. Translation: BAC22515.1.
AY039651 mRNA. Translation: AAK71880.1.
BC098649 mRNA. Translation: AAH98649.1.
AF400653 mRNA. Translation: AAM90639.1.
AF309631 mRNA. Translation: AAL09446.1.
RefSeqiNP_570841.2. NM_130828.2.
UniGeneiRn.86433.

Genome annotation databases

EnsembliENSRNOT00000005425; ENSRNOP00000005425; ENSRNOG00000004030. [Q8K4G9-1]
GeneIDi170672.
KEGGirno:170672.
UCSCiRGD:620461. rat. [Q8K4G9-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB091379 mRNA. Translation: BAC22515.1.
AY039651 mRNA. Translation: AAK71880.1.
BC098649 mRNA. Translation: AAH98649.1.
AF400653 mRNA. Translation: AAM90639.1.
AF309631 mRNA. Translation: AAL09446.1.
RefSeqiNP_570841.2. NM_130828.2.
UniGeneiRn.86433.

3D structure databases

ProteinModelPortaliQ8K4G9.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000005425.

Proteomic databases

PaxDbiQ8K4G9.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000005425; ENSRNOP00000005425; ENSRNOG00000004030. [Q8K4G9-1]
GeneIDi170672.
KEGGirno:170672.
UCSCiRGD:620461. rat. [Q8K4G9-1]

Organism-specific databases

CTDi7827.
RGDi620461. Nphs2.

Phylogenomic databases

eggNOGiKOG2621. Eukaryota.
COG0330. LUCA.
GeneTreeiENSGT00550000074454.
HOGENOMiHOG000217040.
HOVERGENiHBG004815.
InParanoidiQ8K4G9.
KOiK18268.
OMAiWFCIKVV.
OrthoDBiEOG78D7KJ.
PhylomeDBiQ8K4G9.
TreeFamiTF105750.

Enzyme and pathway databases

ReactomeiR-RNO-373753. Nephrin interactions.

Miscellaneous databases

NextBioi621175.
PROiQ8K4G9.

Family and domain databases

InterProiIPR001107. Band_7.
IPR028509. Podocin.
IPR001972. Stomatin_fam.
[Graphical view]
PANTHERiPTHR10264. PTHR10264. 1 hit.
PTHR10264:SF23. PTHR10264:SF23. 1 hit.
PfamiPF01145. Band_7. 1 hit.
[Graphical view]
PRINTSiPR00721. STOMATIN.
SMARTiSM00244. PHB. 1 hit.
[Graphical view]
SUPFAMiSSF117892. SSF117892. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Characterization of a proteinuria susceptibe gene, Pur1, locus on rat chromosome 13."
    Morita H., Yoshimura A., Makino R., Inui K., Nakao N., Usami T., Roselli S., Antignac C., Matsuyama M., Ideura T.
    Submitted (SEP-2002) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
  2. "Cloning of rat homologue of podocin: expression in proteinuric States and in developing glomeruli."
    Kawachi H., Koike H., Kurihara H., Sakai T., Shimizu F.
    J. Am. Soc. Nephrol. 14:46-56(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
    Strain: Wistar.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Tissue: Kidney.
  4. "Rat NPHS2, partial cDNA sequence."
    Kwon T., Kim M.J., Kim D.J.
    Submitted (JUL-2001) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 74-319 (ISOFORM 2).
  5. "NPHS2 mutations in FSGS."
    Tsukaguchi H., Pollak M.R.
    Submitted (SEP-2000) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 88-319 (ISOFORM 1).
  6. "Nuclear relocation of the nephrin and CD2AP-binding protein dendrin promotes apoptosis of podocytes."
    Asanuma K., Campbell K.N., Kim K., Faul C., Mundel P.
    Proc. Natl. Acad. Sci. U.S.A. 104:10134-10139(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH DDN.

Entry informationi

Entry nameiPODO_RAT
AccessioniPrimary (citable) accession number: Q8K4G9
Secondary accession number(s): Q4G090
, Q80WP2, Q8CJE4, Q920E2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 23, 2003
Last sequence update: April 23, 2003
Last modified: January 20, 2016
This is version 86 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.