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Protein

Actin-binding LIM protein 1

Gene

Ablim1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

May act as scaffold protein (By similarity). May play a role in the development of the retina. Has been suggested to play a role in axon guidance.By similarity2 Publications

GO - Molecular functioni

  • actin binding Source: MGI
  • zinc ion binding Source: InterPro

GO - Biological processi

  • axon guidance Source: MGI
  • cilium assembly Source: MGI
  • cytoskeleton organization Source: InterPro
  • lamellipodium assembly Source: MGI
  • positive regulation of transcription from RNA polymerase II promoter Source: MGI
Complete GO annotation...

Keywords - Ligandi

Actin-binding, Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Actin-binding LIM protein 1
Short name:
abLIM-1
Alternative name(s):
Actin-binding LIM protein family member 1
Gene namesi
Name:Ablim1
Synonyms:Ablim, Kiaa0059
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 19

Organism-specific databases

MGIiMGI:1194500. Ablim1.

Subcellular locationi

  • Cytoplasm 1 Publication
  • Cytoplasmcytoskeleton 1 Publication

  • Note: In a striped pattern along the myofibril axis in cardiac myocytes. Associated with the cytoskeleton.

GO - Cellular componenti

  • actin cytoskeleton Source: MGI
  • cytoplasm Source: UniProtKB-SubCell
  • lamellipodium Source: MGI
  • postsynaptic density Source: MGI
  • stress fiber Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000756981 – 861Actin-binding LIM protein 1Add BLAST861

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei216PhosphoserineCombined sources1
Modified residuei411PhosphoserineCombined sources1
Modified residuei417PhosphotyrosineBy similarity1
Modified residuei440PhosphotyrosineCombined sources1
Modified residuei466PhosphoserineCombined sources1
Modified residuei470PhosphoserineCombined sources1
Modified residuei475PhosphoserineCombined sources1
Modified residuei477PhosphothreonineBy similarity1
Modified residuei479PhosphoserineCombined sources1
Modified residuei483PhosphotyrosineBy similarity1
Modified residuei496PhosphoserineBy similarity1
Modified residuei499PhosphoserineCombined sources1
Modified residuei502PhosphoserineCombined sources1
Modified residuei582PhosphoserineCombined sources1
Modified residuei671PhosphoserineCombined sources1
Modified residuei723PhosphoserineBy similarity1
Modified residuei738PhosphoserineBy similarity1
Modified residuei760PhosphoserineCombined sources1
Modified residuei789PhosphoserineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ8K4G5.
PeptideAtlasiQ8K4G5.
PRIDEiQ8K4G5.

PTM databases

iPTMnetiQ8K4G5.
PhosphoSitePlusiQ8K4G5.

Expressioni

Tissue specificityi

Isoform 1 is detected in adult retina, where it is highly expressed in the ganglion layer. Detected in rod inner segment. Isoform 2 is highly expressed in adult retina, brain, kidney and heart. Isoform 3 is highly expressed in adult retina, brain, kidney, liver, skeletal muscle, spleen and heart. Detected in embryonic retina, brain, spinal cord, peripheral sensory ganglia and thymus.3 Publications

Developmental stagei

Isoform 1 is detected at low levels starting from E12 and remains constant until birth. After this levels increase strongly and expression remains high in adults. Isoform 2 and isoform 3 are expressed at a constant high level throughout development.2 Publications

Gene expression databases

BgeeiENSMUSG00000025085.
CleanExiMM_ABLIM1.
ExpressionAtlasiQ8K4G5. baseline and differential.
GenevisibleiQ8K4G5. MM.

Interactioni

Subunit structurei

Binds F-actin. Interacts with ABRA (By similarity).By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
Dlg4Q621084EBI-2307994,EBI-300895

GO - Molecular functioni

  • actin binding Source: MGI

Protein-protein interaction databases

BioGridi230492. 1 interactor.
IntActiQ8K4G5. 2 interactors.
MINTiMINT-4086493.
STRINGi10090.ENSMUSP00000078336.

Structurei

3D structure databases

ProteinModelPortaliQ8K4G5.
SMRiQ8K4G5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini97 – 156LIM zinc-binding 1PROSITE-ProRule annotationAdd BLAST60
Domaini156 – 216LIM zinc-binding 2PROSITE-ProRule annotationAdd BLAST61
Domaini224 – 283LIM zinc-binding 3PROSITE-ProRule annotationAdd BLAST60
Domaini283 – 343LIM zinc-binding 4PROSITE-ProRule annotationAdd BLAST61
Domaini793 – 861HPPROSITE-ProRule annotationAdd BLAST69

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili673 – 723Sequence analysisAdd BLAST51

Sequence similaritiesi

Contains 1 HP (headpiece) domain.PROSITE-ProRule annotation
Contains 4 LIM zinc-binding domains.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil, LIM domain, Repeat

Phylogenomic databases

eggNOGiKOG1044. Eukaryota.
ENOG410XRPY. LUCA.
GeneTreeiENSGT00760000119039.
HOGENOMiHOG000285997.
HOVERGENiHBG031499.
InParanoidiQ8K4G5.
KOiK07520.
PhylomeDBiQ8K4G5.

Family and domain databases

Gene3Di1.10.950.10. 1 hit.
2.10.110.10. 4 hits.
InterProiIPR028448. ABLIM1.
IPR032402. AbLIM_anchor.
IPR003128. Villin_headpiece.
IPR001781. Znf_LIM.
[Graphical view]
PANTHERiPTHR24213:SF18. PTHR24213:SF18. 1 hit.
PfamiPF16182. AbLIM_anchor. 1 hit.
PF00412. LIM. 4 hits.
PF02209. VHP. 1 hit.
[Graphical view]
SMARTiSM00132. LIM. 4 hits.
SM00153. VHP. 1 hit.
[Graphical view]
SUPFAMiSSF47050. SSF47050. 1 hit.
PROSITEiPS51089. HP. 1 hit.
PS00478. LIM_DOMAIN_1. 4 hits.
PS50023. LIM_DOMAIN_2. 4 hits.
[Graphical view]

Sequences (5)i

Sequence statusi: Complete.

This entry describes 5 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8K4G5-1) [UniParc]FASTAAdd to basket
Also known as: AbLIM-L

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MPSLLGLKCL GKLCSSEIGK VPSPERASLR NSHRRLLIED LSVPETPDPA
60 70 80 90 100
HRRRGTVIHL VYLYSAGCGP PELRFSSYDP SVAHPQDPHH SSEKPVIHCH
110 120 130 140 150
KCGEPCKGEV LRVQTKHFHI KCFTCKVCGC DLAQGGFFIK NGDYLCTLDY
160 170 180 190 200
QRMYGTRCHG CGEFVEGEVV TALGKTYHPN CFACTICKRP FPPGDRVTFN
210 220 230 240 250
GRDCLCQLCA QPMSSSPKEA SCSSNCAGCG RDIKNGQALL ALDKQWHLGC
260 270 280 290 300
FKCKSCGKVL TGEYISKDGS PYCEKDYQGL FGVKCEACHQ FITGKVLEAG
310 320 330 340 350
DKHYHPSCAR CSRCNQMFTE GEEMYLQGST VWHPDCKQST KTEEKLRPPN
360 370 380 390 400
IPRSSSDFFY PKSLIRRTGR SPALQLLSPP CLTNSNKNPR QPTRTSSESI
410 420 430 440 450
YSRPGSSIPG SPGHTIYAKV DNEILDYKDL AAIPKVKAIY DIERPDLITY
460 470 480 490 500
EPFYTSGYED KQERQSLGES PRTLSPTPSA EGYQDVRDRM IHRSTSQGSI
510 520 530 540 550
NSPVYSRHSY TPTTSRSPQH FHRPELLSPG VHRWSPLRTS SFSSTHSDSR
560 570 580 590 600
PNPPFRHHFL PHVKGNEPSS GRNSPLPYRP DSRPLTPTYA QAPKHFHVPD
610 620 630 640 650
QGINIYRKPP IYKQHAALAA QSKASEDIIK FSKFPAAQAP DPNEIPKIET
660 670 680 690 700
DHWPGPPSLA AVGTDPRRRS SGREEDEEEL LRRRQLQEEQ LMKLNSGLGQ
710 720 730 740 750
LILKEEMEKE SRERASLASR YDSPLHSASH APSSKTSSLP GYGKNGLHRP
760 770 780 790 800
VSTDFAQYNS YGDISGGVRD YQTLPDGHMP AVRMDRGVSM PNMLEPKIFP
810 820 830 840 850
YEMLMVTNRG RNKILRDVDR TRLERHLAPE VFWEIFGMSI QEFDKLPLWR
860
RNDMKKKAKL F
Length:861
Mass (Da):96,805
Last modified:October 1, 2002 - v1
Checksum:i5E045F7CEF1D91EF
GO
Isoform 2 (identifier: Q8K4G5-2) [UniParc]FASTAAdd to basket
Also known as: AbLIM-M

The sequence of this isoform differs from the canonical sequence as follows:
     1-81: MPSLLGLKCL...ELRFSSYDPS → MVKEK
     348-391: Missing.
     525-564: Missing.

Show »
Length:701
Mass (Da):78,960
Checksum:i5B5B038DC13E8335
GO
Isoform 3 (identifier: Q8K4G5-3) [UniParc]FASTAAdd to basket
Also known as: AbLIM-S

The sequence of this isoform differs from the canonical sequence as follows:
     1-316: Missing.

Show »
Length:545
Mass (Da):62,004
Checksum:i4AC77999CAE0228B
GO
Isoform 4 (identifier: Q8K4G5-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-81: MPSLLGLKCL...ELRFSSYDPS → MVKEK
     348-391: Missing.
     525-564: Missing.
     616-662: Missing.

Note: No experimental confirmation available.
Show »
Length:654
Mass (Da):74,052
Checksum:i90BFFB081F2BF1D2
GO
Isoform 5 (identifier: Q8K4G5-5) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-77: Missing.
     78-81: YDPS → MSTR
     301-345: DKHYHPSCAR...CKQSTKTEEK → VTEASRTWSH...SAREWFALSA
     346-861: Missing.

Note: No experimental confirmation available.
Show »
Length:268
Mass (Da):29,911
Checksum:i08A6BFFDC99BB56B
GO

Sequence cautioni

The sequence BAC65478 differs from that shown. Reason: Erroneous initiation.Curated

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0121031 – 316Missing in isoform 3. 1 PublicationAdd BLAST316
Alternative sequenceiVSP_0121041 – 81MPSLL…SYDPS → MVKEK in isoform 2 and isoform 4. 2 PublicationsAdd BLAST81
Alternative sequenceiVSP_0121051 – 77Missing in isoform 5. 1 PublicationAdd BLAST77
Alternative sequenceiVSP_01210678 – 81YDPS → MSTR in isoform 5. 1 Publication4
Alternative sequenceiVSP_012107301 – 345DKHYH…KTEEK → VTEASRTWSHIDLRWQGRSE ELRAWRHSIQSLRHQSAREW FALSA in isoform 5. 1 PublicationAdd BLAST45
Alternative sequenceiVSP_012109346 – 861Missing in isoform 5. 1 PublicationAdd BLAST516
Alternative sequenceiVSP_012108348 – 391Missing in isoform 2 and isoform 4. 2 PublicationsAdd BLAST44
Alternative sequenceiVSP_012110525 – 564Missing in isoform 2 and isoform 4. 2 PublicationsAdd BLAST40
Alternative sequenceiVSP_012111616 – 662Missing in isoform 4. 1 PublicationAdd BLAST47

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF404774 mRNA. Translation: AAM73705.1.
AF404775 mRNA. Translation: AAM73706.1.
AF404776 mRNA. Translation: AAM73707.1.
AK122196 mRNA. Translation: BAC65478.1. Different initiation.
AK029371 mRNA. Translation: BAC26424.1.
CCDSiCCDS38027.1. [Q8K4G5-1]
CCDS50478.1. [Q8K4G5-3]
CCDS50479.1. [Q8K4G5-2]
CCDS70964.1. [Q8K4G5-4]
RefSeqiNP_001096647.1. NM_001103177.2. [Q8K4G5-2]
NP_001096648.1. NM_001103178.2.
NP_001277742.1. NM_001290813.1.
NP_001277744.1. NM_001290815.1. [Q8K4G5-4]
NP_848803.3. NM_178688.3.
UniGeneiMm.217161.
Mm.446592.

Genome annotation databases

EnsembliENSMUST00000099294; ENSMUSP00000096897; ENSMUSG00000025085. [Q8K4G5-4]
ENSMUST00000111524; ENSMUSP00000107149; ENSMUSG00000025085. [Q8K4G5-5]
ENSMUST00000111546; ENSMUSP00000107172; ENSMUSG00000025085. [Q8K4G5-2]
GeneIDi226251.
KEGGimmu:226251.
UCSCiuc008hzz.1. mouse. [Q8K4G5-5]
uc033hkz.2. mouse. [Q8K4G5-2]
uc033hld.2. mouse. [Q8K4G5-4]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF404774 mRNA. Translation: AAM73705.1.
AF404775 mRNA. Translation: AAM73706.1.
AF404776 mRNA. Translation: AAM73707.1.
AK122196 mRNA. Translation: BAC65478.1. Different initiation.
AK029371 mRNA. Translation: BAC26424.1.
CCDSiCCDS38027.1. [Q8K4G5-1]
CCDS50478.1. [Q8K4G5-3]
CCDS50479.1. [Q8K4G5-2]
CCDS70964.1. [Q8K4G5-4]
RefSeqiNP_001096647.1. NM_001103177.2. [Q8K4G5-2]
NP_001096648.1. NM_001103178.2.
NP_001277742.1. NM_001290813.1.
NP_001277744.1. NM_001290815.1. [Q8K4G5-4]
NP_848803.3. NM_178688.3.
UniGeneiMm.217161.
Mm.446592.

3D structure databases

ProteinModelPortaliQ8K4G5.
SMRiQ8K4G5.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi230492. 1 interactor.
IntActiQ8K4G5. 2 interactors.
MINTiMINT-4086493.
STRINGi10090.ENSMUSP00000078336.

PTM databases

iPTMnetiQ8K4G5.
PhosphoSitePlusiQ8K4G5.

Proteomic databases

PaxDbiQ8K4G5.
PeptideAtlasiQ8K4G5.
PRIDEiQ8K4G5.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000099294; ENSMUSP00000096897; ENSMUSG00000025085. [Q8K4G5-4]
ENSMUST00000111524; ENSMUSP00000107149; ENSMUSG00000025085. [Q8K4G5-5]
ENSMUST00000111546; ENSMUSP00000107172; ENSMUSG00000025085. [Q8K4G5-2]
GeneIDi226251.
KEGGimmu:226251.
UCSCiuc008hzz.1. mouse. [Q8K4G5-5]
uc033hkz.2. mouse. [Q8K4G5-2]
uc033hld.2. mouse. [Q8K4G5-4]

Organism-specific databases

CTDi3983.
MGIiMGI:1194500. Ablim1.
RougeiSearch...

Phylogenomic databases

eggNOGiKOG1044. Eukaryota.
ENOG410XRPY. LUCA.
GeneTreeiENSGT00760000119039.
HOGENOMiHOG000285997.
HOVERGENiHBG031499.
InParanoidiQ8K4G5.
KOiK07520.
PhylomeDBiQ8K4G5.

Miscellaneous databases

PROiQ8K4G5.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000025085.
CleanExiMM_ABLIM1.
ExpressionAtlasiQ8K4G5. baseline and differential.
GenevisibleiQ8K4G5. MM.

Family and domain databases

Gene3Di1.10.950.10. 1 hit.
2.10.110.10. 4 hits.
InterProiIPR028448. ABLIM1.
IPR032402. AbLIM_anchor.
IPR003128. Villin_headpiece.
IPR001781. Znf_LIM.
[Graphical view]
PANTHERiPTHR24213:SF18. PTHR24213:SF18. 1 hit.
PfamiPF16182. AbLIM_anchor. 1 hit.
PF00412. LIM. 4 hits.
PF02209. VHP. 1 hit.
[Graphical view]
SMARTiSM00132. LIM. 4 hits.
SM00153. VHP. 1 hit.
[Graphical view]
SUPFAMiSSF47050. SSF47050. 1 hit.
PROSITEiPS51089. HP. 1 hit.
PS00478. LIM_DOMAIN_1. 4 hits.
PS50023. LIM_DOMAIN_2. 4 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiABLM1_MOUSE
AccessioniPrimary (citable) accession number: Q8K4G5
Secondary accession number(s): Q80U86
, Q8BIR9, Q8K4G3, Q8K4G4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 7, 2004
Last sequence update: October 1, 2002
Last modified: November 2, 2016
This is version 130 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

Isoform 1 is not necessary for normal axon guidance.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.