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Protein

Latent-transforming growth factor beta-binding protein 4

Gene

Ltbp4

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

May be involved in the assembly, secretion and targeting of TGFB1 to sites at which it is stored and/or activated. May play critical roles in controlling and directing the activity of TGFB1. May have a structural role in the extracellular matrix (ECM) (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

  • hormone secretion Source: MGI
  • regulation of proteolysis Source: UniProtKB
  • transforming growth factor beta receptor signaling pathway Source: MGI

Keywordsi

Molecular functionGrowth factor binding

Enzyme and pathway databases

ReactomeiR-MMU-2129379. Molecules associated with elastic fibres.

Names & Taxonomyi

Protein namesi
Recommended name:
Latent-transforming growth factor beta-binding protein 4
Short name:
LTBP-4
Gene namesi
Name:Ltbp4
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 7

Organism-specific databases

MGIiMGI:1321395. Ltbp4.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Extracellular matrix, Secreted

Pathology & Biotechi

Disruption phenotypei

Mice reveal significant disruption of elastic fibers in multiple tissues. They develop pulmonary septation defects, rectal prolapse, colorectal adenomas, and dilated cardiomyopathy. They survive up to six months (mid-adult age) without major clinical symptoms.2 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 24Sequence analysisAdd BLAST24
ChainiPRO_000000764825 – 1666Latent-transforming growth factor beta-binding protein 4Add BLAST1642

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi152 ↔ 162PROSITE-ProRule annotation
Disulfide bondi156 ↔ 168PROSITE-ProRule annotation
Disulfide bondi170 ↔ 179PROSITE-ProRule annotation
Disulfide bondi288 ↔ 310PROSITE-ProRule annotation
Disulfide bondi297 ↔ 323PROSITE-ProRule annotation
Disulfide bondi311 ↔ 326PROSITE-ProRule annotation
Glycosylationi351N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi360 ↔ 371PROSITE-ProRule annotation
Disulfide bondi366 ↔ 380PROSITE-ProRule annotation
Disulfide bondi382 ↔ 395PROSITE-ProRule annotation
Disulfide bondi408 ↔ 430PROSITE-ProRule annotation
Disulfide bondi417 ↔ 443PROSITE-ProRule annotation
Glycosylationi424N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi431 ↔ 446PROSITE-ProRule annotation
Disulfide bondi432 ↔ 458PROSITE-ProRule annotation
Disulfide bondi592 ↔ 604PROSITE-ProRule annotation
Disulfide bondi599 ↔ 613PROSITE-ProRule annotation
Disulfide bondi615 ↔ 628PROSITE-ProRule annotation
Disulfide bondi634 ↔ 646PROSITE-ProRule annotation
Disulfide bondi641 ↔ 655PROSITE-ProRule annotation
Disulfide bondi657 ↔ 670PROSITE-ProRule annotation
Disulfide bondi676 ↔ 688PROSITE-ProRule annotation
Disulfide bondi683 ↔ 697PROSITE-ProRule annotation
Disulfide bondi699 ↔ 712PROSITE-ProRule annotation
Disulfide bondi718 ↔ 730PROSITE-ProRule annotation
Disulfide bondi725 ↔ 739PROSITE-ProRule annotation
Disulfide bondi741 ↔ 750PROSITE-ProRule annotation
Disulfide bondi757 ↔ 769PROSITE-ProRule annotation
Disulfide bondi764 ↔ 778PROSITE-ProRule annotation
Disulfide bondi780 ↔ 793PROSITE-ProRule annotation
Disulfide bondi799 ↔ 811PROSITE-ProRule annotation
Disulfide bondi806 ↔ 820PROSITE-ProRule annotation
Disulfide bondi822 ↔ 835PROSITE-ProRule annotation
Disulfide bondi881 ↔ 893PROSITE-ProRule annotation
Disulfide bondi887 ↔ 902PROSITE-ProRule annotation
Disulfide bondi904 ↔ 918PROSITE-ProRule annotation
Disulfide bondi924 ↔ 936PROSITE-ProRule annotation
Disulfide bondi930 ↔ 945PROSITE-ProRule annotation
Disulfide bondi947 ↔ 960PROSITE-ProRule annotation
Disulfide bondi966 ↔ 977PROSITE-ProRule annotation
Disulfide bondi972 ↔ 986PROSITE-ProRule annotation
Disulfide bondi988 ↔ 1001PROSITE-ProRule annotation
Disulfide bondi1095 ↔ 1107PROSITE-ProRule annotation
Glycosylationi1097N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi1101 ↔ 1116PROSITE-ProRule annotation
Disulfide bondi1118 ↔ 1131PROSITE-ProRule annotation
Disulfide bondi1225 ↔ 1248PROSITE-ProRule annotation
Disulfide bondi1235 ↔ 1260PROSITE-ProRule annotation
Glycosylationi1242N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi1249 ↔ 1265PROSITE-ProRule annotation
Disulfide bondi1250 ↔ 1277PROSITE-ProRule annotation
Disulfide bondi1299 ↔ 1312PROSITE-ProRule annotation
Disulfide bondi1307 ↔ 1321PROSITE-ProRule annotation
Disulfide bondi1323 ↔ 1336PROSITE-ProRule annotation
Disulfide bondi1342 ↔ 1354PROSITE-ProRule annotation
Disulfide bondi1349 ↔ 1363PROSITE-ProRule annotation
Disulfide bondi1365 ↔ 1378PROSITE-ProRule annotation
Glycosylationi1381N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi1393 ↔ 1417PROSITE-ProRule annotation
Disulfide bondi1403 ↔ 1429PROSITE-ProRule annotation
Disulfide bondi1418 ↔ 1432PROSITE-ProRule annotation
Disulfide bondi1419 ↔ 1444PROSITE-ProRule annotation
Modified residuei1564PhosphothreonineCombined sources1
Disulfide bondi1579 ↔ 1590PROSITE-ProRule annotation
Disulfide bondi1585 ↔ 1599PROSITE-ProRule annotation
Disulfide bondi1601 ↔ 1614PROSITE-ProRule annotation
Disulfide bondi1620 ↔ 1635PROSITE-ProRule annotation
Disulfide bondi1630 ↔ 1644PROSITE-ProRule annotation
Disulfide bondi1646 ↔ 1659PROSITE-ProRule annotation

Post-translational modificationi

Contains hydroxylated asparagine residues.By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein, Phosphoprotein

Proteomic databases

PaxDbiQ8K4G1.
PeptideAtlasiQ8K4G1.
PRIDEiQ8K4G1.

PTM databases

iPTMnetiQ8K4G1.
PhosphoSitePlusiQ8K4G1.

Expressioni

Gene expression databases

BgeeiENSMUSG00000040488.
ExpressionAtlasiQ8K4G1. baseline and differential.
GenevisibleiQ8K4G1. MM.

Interactioni

Subunit structurei

Forms part of the large latent transforming growth factor beta precursor complex; removal is essential for activation of complex. Interacts with LTBP1 AND TGFB1. Binds to FBN1 (By similarity).By similarity

GO - Molecular functioni

Protein-protein interaction databases

IntActiQ8K4G1. 1 interactor.
MINTiMINT-4100656.
STRINGi10090.ENSMUSP00000037536.

Structurei

3D structure databases

ProteinModelPortaliQ8K4G1.
SMRiQ8K4G1.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini148 – 180EGF-like 1PROSITE-ProRule annotationAdd BLAST33
Domaini286 – 338TB 1PROSITE-ProRule annotationAdd BLAST53
Domaini356 – 396EGF-like 2; calcium-bindingPROSITE-ProRule annotationAdd BLAST41
Domaini406 – 458TB 2PROSITE-ProRule annotationAdd BLAST53
Domaini588 – 629EGF-like 3PROSITE-ProRule annotationAdd BLAST42
Domaini630 – 671EGF-like 4; calcium-bindingPROSITE-ProRule annotationAdd BLAST42
Domaini672 – 713EGF-like 5; calcium-bindingPROSITE-ProRule annotationAdd BLAST42
Domaini714 – 751EGF-like 6; calcium-bindingPROSITE-ProRule annotationAdd BLAST38
Domaini753 – 794EGF-like 7; calcium-bindingPROSITE-ProRule annotationAdd BLAST42
Domaini795 – 836EGF-like 8; calcium-bindingPROSITE-ProRule annotationAdd BLAST42
Domaini877 – 919EGF-like 9; calcium-bindingPROSITE-ProRule annotationAdd BLAST43
Domaini920 – 961EGF-like 10; calcium-bindingPROSITE-ProRule annotationAdd BLAST42
Domaini962 – 1002EGF-like 11; calcium-bindingPROSITE-ProRule annotationAdd BLAST41
Domaini1091 – 1132EGF-like 12; calcium-bindingPROSITE-ProRule annotationAdd BLAST42
Domaini1223 – 1277TB 3PROSITE-ProRule annotationAdd BLAST55
Domaini1295 – 1337EGF-like 13; calcium-bindingPROSITE-ProRule annotationAdd BLAST43
Domaini1338 – 1379EGF-like 14; calcium-bindingPROSITE-ProRule annotationAdd BLAST42
Domaini1391 – 1444TB 4PROSITE-ProRule annotationAdd BLAST54
Domaini1575 – 1615EGF-like 15PROSITE-ProRule annotationAdd BLAST41
Domaini1616 – 1660EGF-like 16PROSITE-ProRule annotationAdd BLAST45

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi477 – 608Pro-richAdd BLAST132
Compositional biasi592 – 1160Cys-richAdd BLAST569
Compositional biasi1161 – 1217Pro-richAdd BLAST57
Compositional biasi1449 – 1534Pro-richAdd BLAST86

Sequence similaritiesi

Belongs to the LTBP family.Curated

Keywords - Domaini

EGF-like domain, Repeat, Signal

Phylogenomic databases

eggNOGiKOG1217. Eukaryota.
ENOG410YAPY. LUCA.
GeneTreeiENSGT00760000118806.
HOGENOMiHOG000293153.
HOVERGENiHBG052370.
InParanoidiQ8K4G1.
KOiK08023.
OMAiQVSLNWQ.
OrthoDBiEOG091G009A.
TreeFamiTF317514.

Family and domain databases

Gene3Di2.40.155.10. 1 hit.
3.90.290.10. 4 hits.
InterProiView protein in InterPro
IPR001881. EGF-like_Ca-bd_dom.
IPR013032. EGF-like_CS.
IPR000742. EGF-like_dom.
IPR000152. EGF-type_Asp/Asn_hydroxyl_site.
IPR018097. EGF_Ca-bd_CS.
IPR009017. GFP.
IPR009030. Growth_fac_rcpt_.
IPR017878. TB_dom.
IPR036773. TB_dom_sf.
PfamiView protein in Pfam
PF07645. EGF_CA. 17 hits.
PF00683. TB. 3 hits.
SMARTiView protein in SMART
SM00181. EGF. 20 hits.
SM00179. EGF_CA. 19 hits.
SUPFAMiSSF57184. SSF57184. 5 hits.
SSF57581. SSF57581. 4 hits.
PROSITEiView protein in PROSITE
PS00010. ASX_HYDROXYL. 14 hits.
PS00022. EGF_1. 2 hits.
PS01186. EGF_2. 12 hits.
PS50026. EGF_3. 16 hits.
PS01187. EGF_CA. 17 hits.
PS51364. TB. 4 hits.

Sequences (3)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8K4G1-1) [UniParc]FASTAAdd to basket
Also known as: LTBP4L

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MRRPGLGGPC PLLLLLLLPA ATSASGSSPS PSPSPIEKAV VPSHQAGVAA
60 70 80 90 100
CHCCLDQTPK SSRCTRASCR VRNCPPAKCT GLEGCLTPTP SVPSPSRSPV
110 120 130 140 150
EKSQVSLNWQ PLTLQEARAL LRQRRPRGPW ARALLKRRPP HRAPAGQARV
160 170 180 190 200
LCPLICHNGG VCVKPDRCLC PPDFAGKFCQ LHSSGARPPA PAMPGLTRSV
210 220 230 240 250
YTMPLANHRD DEHGVASMVS VHVEHPQEAS VVVHQVERVS GPWEEANPEA
260 270 280 290 300
LARAEAAARA EAAAPYTVLA QSAPREDGYS DASGFGYCFR ELRGSECASP
310 320 330 340 350
LPGLRTQEVC CRGEGLAWGV HDCHPCAEHL RNSNQVSGPN GPCPPGFERV
360 370 380 390 400
NGSCVDVDEC ATGGRCQHGE CANTRGGYTC VCPDGFLLDS SRSSCISQHV
410 420 430 440 450
ISEAKGPCYR VLHDGGCSLP ILRNITKQIC CCSRVGKAWG RGCQLCPPYG
460 470 480 490 500
SEGFREICPA GPGYHYSASD LRYNTRPLNQ DPPRVTFNQP RVPPATPRPP
510 520 530 540 550
TGFLPTRRPE PRPDPGPQPE PRPRPEPRPR PESRPRPEPR PRPEPRPQPE
560 570 580 590 600
SQPRPESRPR PESQPWPEFP LPSIPAWTGP EIPESGPSSS MCQRNPQVCG
610 620 630 640 650
PGRCVPRPSG YTCACDPGFR LGPQGTRCID IDECRRVPTP CAPGRCENTP
660 670 680 690 700
GSFRCVCGTG FQAGPRATEC LDVDECRRVP PPCDRGRCEN TPGSFLCVCP
710 720 730 740 750
AGYQAAPHGA SCQDVDECTQ SPGLCGRGVC ENLPGSFRCV CPAGFRGSAC
760 770 780 790 800
EEDVDECAQQ PPPCGPGRCD NTAGSFHCAC PAGFRSRGPG APCQDVDECS
810 820 830 840 850
RSPSPCAYGR CENTEGSFKC VCPTGFQPNA AGSECEDVDE CENRLACPGQ
860 870 880 890 900
ECVNSPGSFQ CRACPVGHHL HRGRCTDVDE CSSGTPCGLH GQCTNTKGSF
910 920 930 940 950
HCSCSTGYRA PSGQPGPCAD INECLEGDFC FPHGECLNTD GSFTCTCAPG
960 970 980 990 1000
YRPGPRGASC LDVDECSEED LCQSGICTNT DGSFECICPP GHRAGPDLAS
1010 1020 1030 1040 1050
CLDIDECRER GPALCGSQRC ENSPGSYRCV RDCDPGYHPG PEGTCDDIDE
1060 1070 1080 1090 1100
CREYGSAICG AQRCENTPGS YRCTPACDPG YQPTPGGGCQ DVDECRNRSF
1110 1120 1130 1140 1150
CGAHAMCQNL PGSFQCVCDQ GYEGARDGRH CVDVNECETL QGVCGSALCE
1160 1170 1180 1190 1200
NVEGSFLCVC PNSPEEFDPM TGRCVPPRAP AGTFPGSQPQ APASPSLPAR
1210 1220 1230 1240 1250
PPAPPPPRRP SPPRQGPVSS GRRECYFDTA APDACDNILA RNVTWQECCC
1260 1270 1280 1290 1300
TVGEGWGSGC RIQQCPGTET AEYQSLCPHG RGYLVPSGDL SARRDVDECQ
1310 1320 1330 1340 1350
LFQDQVCKSG VCVNTAPGYS CYCSNGFYYH AHRLECVDND ECADEEPACE
1360 1370 1380 1390 1400
GGRCVNTVGS YHCTCEPPLV LDGSRRRCVS NESQSLDDNL GVCWQEVGPD
1410 1420 1430 1440 1450
LVCSRPRLDR QATYTECCCL YGEAWGMDCA LCPAQDSDDF EALCNVLRPP
1460 1470 1480 1490 1500
AYGPPRPGGF GIPYEYGPDI GPPYQSLPYG PDLYPPPVLP YDPYPPPPGP
1510 1520 1530 1540 1550
FARREAPYGA PPFDMPDFED DGGPYGESET PDPPSRGTGW PYRSRDTRGS
1560 1570 1580 1590 1600
FPEPEESSER GSYTGALSEP YEGLEAEECG ILDGCPHGRC VRVPEGFTCD
1610 1620 1630 1640 1650
CFDGYRLDIT RMSCVDVNEC DEAEATSPLC VNARCVNTDG SFRCICRPGF
1660
APTHQPHHCA PARPRA
Length:1,666
Mass (Da):178,642
Last modified:July 27, 2011 - v2
Checksum:i3784110C2B714E7D
GO
Isoform 2 (identifier: Q8K4G1-2) [UniParc]FASTAAdd to basket
Also known as: LTBP4S

The sequence of this isoform differs from the canonical sequence as follows:
     1-66: Missing.
     67-150: ASCRVRNCPP...HRAPAGQARV → MAGGAQLLWV...APGGPGFRAF

Show »
Length:1,600
Mass (Da):171,304
Checksum:i988B6E43BA69E164
GO
Isoform 3 (identifier: Q8K4G1-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     60-150: KSSRCTRASC...HRAPAGQARV → RHEAALQPWR...APGGPGFRAF

Note: Sequence incomplete.
Show »
Length:1,666
Mass (Da):178,036
Checksum:i96DDC2CF571CC22D
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti361A → S in AAH59016 (PubMed:15489334).Curated1
Sequence conflicti625G → D in AAN04661 (PubMed:12208849).Curated1
Sequence conflicti1631V → I in AAN04661 (PubMed:12208849).Curated1
Sequence conflicti1631V → I in AAN04662 (PubMed:12208849).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0092481 – 66Missing in isoform 2. 1 PublicationAdd BLAST66
Alternative sequenceiVSP_00924960 – 150KSSRC…GQARV → RHEAALQPWRAARSCSGCRY WCCWRSWGRSPYWADPESVF ACASPPLCAACVASTGPLAP AVPRPARPATPPVDSGAPGG AAPGGPGFRAF in isoform 3. 1 PublicationAdd BLAST91
Alternative sequenceiVSP_00925067 – 150ASCRV…GQARV → MAGGAQLLWVSLLVLLAQLG PQPVLGRPRERLRVRFTPAV CGLRCIHGPTGSRCTPTCAP RNATSVDSGAPGGAAPGGPG FRAF in isoform 2. 1 PublicationAdd BLAST84

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF410798 mRNA. Translation: AAN04661.1.
AF410799 mRNA. Translation: AAN04662.1.
AC157561 Genomic DNA. No translation available.
BC059016 mRNA. Translation: AAH59016.1.
CCDSiCCDS21017.1. [Q8K4G1-1]
CCDS52158.1. [Q8K4G1-2]
RefSeqiNP_001107021.1. NM_001113549.1. [Q8K4G1-2]
NP_783572.2. NM_175641.2. [Q8K4G1-1]
UniGeneiMm.272251.

Genome annotation databases

EnsembliENSMUST00000038618; ENSMUSP00000037536; ENSMUSG00000040488. [Q8K4G1-1]
ENSMUST00000121175; ENSMUSP00000113674; ENSMUSG00000040488. [Q8K4G1-2]
GeneIDi108075.
KEGGimmu:108075.
UCSCiuc009fvs.2. mouse. [Q8K4G1-2]
uc009fvt.2. mouse. [Q8K4G1-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiLTBP4_MOUSE
AccessioniPrimary (citable) accession number: Q8K4G1
Secondary accession number(s): E9QPD9, Q8K4G0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 16, 2004
Last sequence update: July 27, 2011
Last modified: October 25, 2017
This is version 141 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families