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Protein

m7GpppX diphosphatase

Gene

Dcps

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Decapping scavenger enzyme that catalyzes the cleavage of a residual cap structure following the degradation of mRNAs by the 3'->5' exosome-mediated mRNA decay pathway. Hydrolyzes cap analog structures like 7-methylguanosine nucleoside triphosphate (m7GpppG) with up to 10 nucleotide substrates (small capped oligoribonucleotides) and specifically releases 5'-phosphorylated RNA fragments and 7-methylguanosine monophosphate (m7GMP). Cleaves cap analog structures like tri-methyl guanosine nucleoside triphosphate (m3(2,2,7)GpppG) with very poor efficiency. Does not hydrolyze unmethylated cap analog (GpppG) and shows no decapping activity on intact m7GpppG-capped mRNA molecules longer than 25 nucleotides. Does not hydrolyze 7-methylguanosine diphosphate (m7GDP) to m7GMP. May also play a role in the 5'->3 mRNA decay pathway; m7GDP, the downstream product released by the 5'->3' mRNA mediated decapping activity, may be also converted by DCPS to m7GMP. Binds to m7GpppG and strongly to m7GDP. Plays a role in first intron splicing of pre-mRNAs. Inhibits activation-induced cell death.By similarity

Catalytic activityi

A 5'-(N7-methyl 5'-triphosphoguanosine)-[mRNA] + H2O = N7-methylguanosine 5'-phosphate + a 5'-diphospho-[mRNA].

Enzyme regulationi

The hydrolytic product 7-methylguanosine diphosphate (m7GDP) efficiently inhibits the decapping scavenger activity and acts as a competitive inhibitor in vitro. Inhibited by 2,4-diaminoquinazoline.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei174SubstrateBy similarity1
Binding sitei184SubstrateBy similarity1
Binding sitei204SubstrateBy similarity1
Binding sitei206SubstrateBy similarity1
Active sitei276NucleophileBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase
Biological processmRNA processing, mRNA splicing

Names & Taxonomyi

Protein namesi
Recommended name:
m7GpppX diphosphatase (EC:3.6.1.59)
Alternative name(s):
DCS-1
Decapping scavenger enzyme
Hint-related 7meGMP-directed hydrolase
Histidine triad nucleotide-binding protein 5
Histidine triad protein member 5
Short name:
HINT-5
Scavenger mRNA-decapping enzyme DcpS
Gene namesi
Name:Dcps
Synonyms:Dcs1, Hint5
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi

Organism-specific databases

RGDi628887 Dcps

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00001097972 – 336m7GpppX diphosphataseAdd BLAST335

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineBy similarity1
Modified residuei23PhosphoserineBy similarity1
Modified residuei137N6-acetyllysineBy similarity1
Modified residuei141N6-acetyllysineBy similarity1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiQ8K4F7
PRIDEiQ8K4F7

PTM databases

iPTMnetiQ8K4F7
PhosphoSitePlusiQ8K4F7

Interactioni

Subunit structurei

Homodimer. Associates with components of the exosome multienzyme ribonuclease complex, such as EXOSC3 and EXOSC4. Interacts with NDOR1.By similarity

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000013763

Structurei

3D structure databases

ProteinModelPortaliQ8K4F7
SMRiQ8K4F7
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni267 – 278Substrate bindingBy similarityAdd BLAST12

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi9 – 12nuclear localization signal (NLS)By similarity4
Motifi141 – 153nuclear export sequence (NES)By similarityAdd BLAST13
Motifi274 – 278Histidine triad motifBy similarity5

Domaini

The C-terminal histidine triad (HIT) motif and the N-terminal domain are required for the decapping activity. The N-terminus is necessary but not sufficient for binding cap structures.By similarity

Sequence similaritiesi

Belongs to the HIT family.Curated

Phylogenomic databases

eggNOGiKOG3969 Eukaryota
COG5075 LUCA
HOGENOMiHOG000182411
HOVERGENiHBG051322
InParanoidiQ8K4F7
KOiK12584
PhylomeDBiQ8K4F7

Family and domain databases

Gene3Di3.30.200.40, 1 hit
3.30.428.10, 1 hit
InterProiView protein in InterPro
IPR008594 DcpS/DCS2
IPR019808 Histidine_triad_CS
IPR036265 HIT-like_sf
IPR011145 Scavenger_mRNA_decap_enz_N
PANTHERiPTHR12978 PTHR12978, 1 hit
PfamiView protein in Pfam
PF05652 DcpS, 1 hit
PIRSFiPIRSF028973 Scavenger_mRNA_decap_enz, 1 hit
SUPFAMiSSF102860 SSF102860, 1 hit
SSF54197 SSF54197, 1 hit
PROSITEiView protein in PROSITE
PS00892 HIT_1, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q8K4F7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MADTAPQLKR KREQEAEEAE TPSTEEKEAG VGNGTSAPVR LPFSGFRVQK
60 70 80 90 100
VLRESARDKI IFLHGKVNED SGDTHGEDAV VILEKTPFQV EHVAQLLTGN
110 120 130 140 150
PELKLQFSND IYSTYNLFPP RHLSDIKTTV VYPASEKHLQ KYMRQDLRLI
160 170 180 190 200
RETGDDYRSL TLPYLESQSL SIQWVYNILD KKAEADRIVF ENPDPSDGFV
210 220 230 240 250
LIPDLKWNQQ QLDDLYLIAI CHRRGIRSLR DLTPEHLPLL RNILREGQEA
260 270 280 290 300
ILKRYQVTGD RLRVYLHYLP SYYHLHVHFT ALGFEAPGSG VERAHLLAEV
310 320 330
IENLECDPKH YQRRTLTFAL RTDDPLLQLL QKAQQP
Length:336
Mass (Da):38,714
Last modified:October 1, 2002 - v1
Checksum:iAEFA4D106FC5B6AE
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF411249 mRNA Translation: AAN03664.1
RefSeqiNP_695214.1, NM_153302.1
UniGeneiRn.162991

Genome annotation databases

GeneIDi266605
KEGGirno:266605
UCSCiRGD:628887 rat

Entry informationi

Entry nameiDCPS_RAT
AccessioniPrimary (citable) accession number: Q8K4F7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 1, 2005
Last sequence update: October 1, 2002
Last modified: March 28, 2018
This is version 96 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

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