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Protein

Probable 28S rRNA (cytosine-C(5))-methyltransferase

Gene

Nsun5

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

S-adenosyl-L-methionine-dependent methyltransferase that specifically methylates the C5 position of a cytosine in 28S rRNA.By similarity

Catalytic activityi

S-adenosyl-L-methionine + cytosine in 28S rRNA = S-adenosyl-L-homocysteine + C(5)-methylcytosine in 28S rRNA.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei258 – 2581S-adenosyl-L-methioninePROSITE-ProRule annotation
Binding sitei263 – 2631S-adenosyl-L-methioninePROSITE-ProRule annotation
Binding sitei305 – 3051S-adenosyl-L-methioninePROSITE-ProRule annotation
Active sitei359 – 3591NucleophilePROSITE-ProRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

rRNA processing

Keywords - Ligandi

RNA-binding, S-adenosyl-L-methionine

Names & Taxonomyi

Protein namesi
Recommended name:
Probable 28S rRNA (cytosine-C(5))-methyltransferase (EC:2.1.1.-)
Alternative name(s):
NOL1/NOP2/Sun domain family member 5
Williams-Beuren syndrome chromosomal region 20A protein homolog
Gene namesi
Name:Nsun5
Synonyms:Wbscr20, Wbscr20a
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 5

Organism-specific databases

MGIiMGI:2140844. Nsun5.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemovedBy similarity
Chaini2 – 465464Probable 28S rRNA (cytosine-C(5))-methyltransferasePRO_0000261670Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylglycineBy similarity
Modified residuei167 – 1671PhosphoserineBy similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ8K4F6.
MaxQBiQ8K4F6.
PaxDbiQ8K4F6.
PeptideAtlasiQ8K4F6.
PRIDEiQ8K4F6.

PTM databases

iPTMnetiQ8K4F6.
PhosphoSiteiQ8K4F6.

Expressioni

Gene expression databases

BgeeiENSMUSG00000000916.
CleanExiMM_NSUN5.
ExpressionAtlasiQ8K4F6. baseline and differential.
GenevisibleiQ8K4F6. MM.

Interactioni

Protein-protein interaction databases

BioGridi221497. 1 interaction.
IntActiQ8K4F6. 1 interaction.
MINTiMINT-4607734.
STRINGi10090.ENSMUSP00000000940.

Structurei

3D structure databases

ProteinModelPortaliQ8K4F6.
SMRiQ8K4F6. Positions 133-452.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni234 – 2407S-adenosyl-L-methionine bindingPROSITE-ProRule annotation

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. RsmB/NOP family.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG2360. Eukaryota.
COG0144. LUCA.
GeneTreeiENSGT00550000074709.
HOGENOMiHOG000203203.
HOVERGENiHBG062278.
InParanoidiQ8K4F6.
KOiK15264.
OMAiLVYDSKF.
OrthoDBiEOG091G0TW9.
PhylomeDBiQ8K4F6.
TreeFamiTF314285.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
InterProiIPR001678. MeTrfase_RsmB/NOP2.
IPR023267. RCMT.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PfamiPF01189. Methyltr_RsmB-F. 1 hit.
[Graphical view]
PRINTSiPR02008. RCMTFAMILY.
SUPFAMiSSF53335. SSF53335. 1 hit.
PROSITEiPS51686. SAM_MT_RSMB_NOP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q8K4F6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGLYAAAAAV LAGVESRQGS LKGLVYSSNF QNLKQLYALV CETQRYSAVL
60 70 80 90 100
DAVIASAGLL RAEKKLRPHL AKVLVYELLL GKGFRGGGGR WKALLGRHQA
110 120 130 140 150
RLKAELARLK VHRGVSRNED LLQESSRPGQ AYQVPRFVRV NTLKTRPEDA
160 170 180 190 200
IDYFKRQGFS YQGRASSLED LRALKGQHFL LDPLLPELLV FPAQTDLHEH
210 220 230 240 250
PLYRAGHLIL QDKASCLPAM LLSPPPGSHV IDACAAPGNK TSYIAALLKN
260 270 280 290 300
QGKIFAFDQD AKRLAAMATL VARAGVSCCE LAEKDFLTVS PSDQRYSQVQ
310 320 330 340 350
YILLDPSCSG SGMLSRQLEE HGEGTPSKER LQALAGFQQR ALCHALRFPS
360 370 380 390 400
LQRLVYSTCS LCQEENEDVV QEALQHNSGT FRLAPVLPTW PHRGLSTFPG
410 420 430 440 450
SEHCLRASPE TTLTGGFFIA VFERAEVVPT PAPQTDAMDP EPLSQVPKRK
460
RRRKAAVGAS MQPST
Length:465
Mass (Da):51,030
Last modified:November 28, 2006 - v2
Checksum:iE0545B5290D46D9A
GO

Sequence cautioni

The sequence AAH51209 differs from that shown. Reason: Erroneous initiation. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti40 – 401V → M in BAE33793 (PubMed:16141072).Curated
Sequence conflicti130 – 1301Q → H in AAM62311 (PubMed:12073013).Curated
Sequence conflicti396 – 3961S → N in BAE33793 (PubMed:16141072).Curated
Sequence conflicti443 – 4431L → F in AAH51209 (PubMed:15489334).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF412029 mRNA. Translation: AAM62311.1.
AK079400 mRNA. Translation: BAC37634.1.
AK149521 mRNA. Translation: BAE28935.1.
AK156651 mRNA. Translation: BAE33793.1.
BC051209 mRNA. Translation: AAH51209.1. Different initiation.
CCDSiCCDS19740.1.
RefSeqiNP_663389.2. NM_145414.2.
UniGeneiMm.281189.

Genome annotation databases

EnsembliENSMUST00000000940; ENSMUSP00000000940; ENSMUSG00000000916.
GeneIDi100609.
KEGGimmu:100609.
UCSCiuc008zyh.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF412029 mRNA. Translation: AAM62311.1.
AK079400 mRNA. Translation: BAC37634.1.
AK149521 mRNA. Translation: BAE28935.1.
AK156651 mRNA. Translation: BAE33793.1.
BC051209 mRNA. Translation: AAH51209.1. Different initiation.
CCDSiCCDS19740.1.
RefSeqiNP_663389.2. NM_145414.2.
UniGeneiMm.281189.

3D structure databases

ProteinModelPortaliQ8K4F6.
SMRiQ8K4F6. Positions 133-452.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi221497. 1 interaction.
IntActiQ8K4F6. 1 interaction.
MINTiMINT-4607734.
STRINGi10090.ENSMUSP00000000940.

PTM databases

iPTMnetiQ8K4F6.
PhosphoSiteiQ8K4F6.

Proteomic databases

EPDiQ8K4F6.
MaxQBiQ8K4F6.
PaxDbiQ8K4F6.
PeptideAtlasiQ8K4F6.
PRIDEiQ8K4F6.

Protocols and materials databases

DNASUi100609.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000000940; ENSMUSP00000000940; ENSMUSG00000000916.
GeneIDi100609.
KEGGimmu:100609.
UCSCiuc008zyh.1. mouse.

Organism-specific databases

CTDi55695.
MGIiMGI:2140844. Nsun5.

Phylogenomic databases

eggNOGiKOG2360. Eukaryota.
COG0144. LUCA.
GeneTreeiENSGT00550000074709.
HOGENOMiHOG000203203.
HOVERGENiHBG062278.
InParanoidiQ8K4F6.
KOiK15264.
OMAiLVYDSKF.
OrthoDBiEOG091G0TW9.
PhylomeDBiQ8K4F6.
TreeFamiTF314285.

Miscellaneous databases

ChiTaRSiNsun5. mouse.
PROiQ8K4F6.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000000916.
CleanExiMM_NSUN5.
ExpressionAtlasiQ8K4F6. baseline and differential.
GenevisibleiQ8K4F6. MM.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
InterProiIPR001678. MeTrfase_RsmB/NOP2.
IPR023267. RCMT.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PfamiPF01189. Methyltr_RsmB-F. 1 hit.
[Graphical view]
PRINTSiPR02008. RCMTFAMILY.
SUPFAMiSSF53335. SSF53335. 1 hit.
PROSITEiPS51686. SAM_MT_RSMB_NOP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiNSUN5_MOUSE
AccessioniPrimary (citable) accession number: Q8K4F6
Secondary accession number(s): Q3U0Q8, Q80WG3, Q8C568
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 28, 2006
Last sequence update: November 28, 2006
Last modified: September 7, 2016
This is version 98 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.