ID   LX15B_RAT               Reviewed;         677 AA.
AC   Q8K4F2;
DT   01-MAR-2005, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-2002, sequence version 1.
DT   16-JUN-2009, entry version 50.
DE   RecName: Full=Arachidonate 15-lipoxygenase type II;
DE            Short=15-LOX-2;
DE            EC=1.13.11.33;
GN   Name=Alox15b;
OS   Rattus norvegicus (Rat).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Sciurognathi;
OC   Muroidea; Muridae; Murinae; Rattus.
OX   NCBI_TaxID=10116;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RA   Boeglin W.E., Schneider C., Brash A.R.;
RT   "A 15-lipoxygenase in the rat, homolog of human 15-lipoxygenase-2 and
RT   mouse 8-lipoxygenase.";
RL   Submitted (AUG-2001) to the EMBL/GenBank/DDBJ databases.
CC   -!- CATALYTIC ACTIVITY: Arachidonate + O(2) = (5Z,8Z,11Z,13E)-(15S)-
CC       15-hydroperoxyicosa-5,8,11,13-tetraenoate.
CC   -!- COFACTOR: Binds 1 iron ion per subunit (By similarity).
CC   -!- PATHWAY: Lipid metabolism; leukotriene D4 biosynthesis.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm (By similarity).
CC   -!- SIMILARITY: Belongs to the lipoxygenase family.
CC   -!- SIMILARITY: Contains 1 lipoxygenase domain.
CC   -!- SIMILARITY: Contains 1 PLAT domain.
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DR   EMBL; AF415240; AAN03708.1; -; mRNA.
DR   IPI; IPI00325845; -.
DR   RefSeq; NP_695213.1; -.
DR   UniGene; Rn.86437; -.
DR   HSSP; P12530; 1LOX.
DR   Ensembl; ENSRNOG00000007778; Rattus norvegicus.
DR   GeneID; 266604; -.
DR   KEGG; rno:266604; -.
DR   RGD; 628809; Alox15b.
DR   HOVERGEN; Q8K4F2; -.
DR   BRENDA; 1.13.11.33; 248.
DR   NextBio; 624420; -.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0050473; F:arachidonate 15-lipoxygenase activity; ISS:UniProtKB.
DR   GO; GO:0005506; F:iron ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0016165; F:lipoxygenase activity; IEA:InterPro.
DR   GO; GO:0019370; P:leukotriene biosynthetic process; IEA:UniProtKB-KW.
DR   GO; GO:0045786; P:negative regulation of cell cycle; ISS:UniProtKB.
DR   GO; GO:0008285; P:negative regulation of cell proliferation; ISS:UniProtKB.
DR   GO; GO:0045926; P:negative regulation of growth; ISS:UniProtKB.
DR   GO; GO:0055114; P:oxidation reduction; IEA:UniProtKB-KW.
DR   InterPro; IPR000907; LipOase.
DR   InterPro; IPR013819; LipOase_C.
DR   InterPro; IPR001024; LipOase_LH2.
DR   InterPro; IPR001885; LipOase_mml.
DR   Gene3D; G3DSA:2.60.60.20; Lipase_LipOase; 1.
DR   PANTHER; PTHR11771; LipOase; 1.
DR   Pfam; PF00305; Lipoxygenase; 1.
DR   Pfam; PF01477; PLAT; 1.
DR   PRINTS; PR00087; LIPOXYGENASE.
DR   PRINTS; PR00467; MAMLPOXGNASE.
DR   SMART; SM00308; LH2; 1.
DR   PROSITE; PS00711; LIPOXYGENASE_1; 1.
DR   PROSITE; PS00081; LIPOXYGENASE_2; 1.
DR   PROSITE; PS51393; LIPOXYGENASE_3; 1.
DR   PROSITE; PS50095; PLAT; 1.
PE   2: Evidence at transcript level;
KW   Cytoplasm; Dioxygenase; Iron; Leukotriene biosynthesis; Metal-binding;
KW   Oxidoreductase.
FT   CHAIN         1    677       Arachidonate 15-lipoxygenase type II.
FT                                /FTId=PRO_0000220702.
FT   DOMAIN        2    125       PLAT.
FT   DOMAIN      126    677       Lipoxygenase.
FT   METAL       374    374       Iron; catalytic (By similarity).
FT   METAL       379    379       Iron; catalytic (By similarity).
FT   METAL       554    554       Iron; catalytic (By similarity).
FT   METAL       677    677       Iron; via carboxylate; catalytic (By
FT                                similarity).
SQ   SEQUENCE   677 AA;  76145 MW;  49A47B47C491B8B1 CRC64;
     MAKFRVRVST GEACGAGTWD KVSVSIVGTH GESPLVPLDH LGKEFSAGAE EDFEVTLPQD
     VGTVLMLRIH KAPPEAPLPL LSFPPDAWYC RWFELEWLPG AALRFPCYQW LEGAGELVLR
     EGAAKVSWQD HHRTLQDQRQ KELESRKDMY SWKTYIEGWP HCLDHETVKD LDLNIKYSAM
     KNAKFFFKAQ SAFTELKFKG LLDRTGLWRS LREMKRMFNF HNTPAAEYVF AHWQEDAFFA
     SQFLNGLNPV LIRRCRRLPE NFPVTDEMVA PVLGPGTSLQ AELEKGSLFL VDHGILSGVQ
     TNVINGKPQF SAAPMTLLYQ SPGSGPLLPI AIQLKQTPGP DNPIFLPSDD KWDWLLAKTW
     VRNAEFSIHE ALTHLLHAHL IPEVFALATL RQLPHCHPLF KLLIPHTRYT LHINTLAREL
     LIAPGKVVDK STGLGIGGFS DLIKRNMEQL SYSVLCLPED IRARDVGDLP GYYYRDDGMQ
     IWSAIRSFVS EIVDIYYPSD ASVRDDQELQ AWVGEIFSEG FLSQESSGMP SLLDTQEALV
     QYVTMVIFTC SAKHAAVSAS QFDSCVWMPN LPPSMQLPPP TSKGQASPEG FIATLPAVNA
     TCDVIIALWL LSKEPGDRRP LGHYPDEHFT EEVPRRSIAA FQRKLIQISS GIRKRNQSLA
     LPYTYLDPPL IENSVSI
//