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Protein

Arachidonate 15-lipoxygenase B

Gene

Alox15b

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Non-heme iron-containing dioxygenase that catalyzes the stereo-specific peroxidation of free and esterified polyunsaturated fatty acids generating a spectrum of bioactive lipid mediators. Converts arachidonic acid to 15S-hydroperoxyeicosatetraenoic acid/(15S)-HPETE. Also acts on linoleic acid to produce 13-hydroxyoctadecadienoic acid/13-HPODE. Has no detectable 8S-lipoxygenase activity but reacts with (8S)-HPETE to produce (8S,15S)-diHPETE. May regulate progression through the cell cycle and cell proliferation. May also regulate cytokine secretion by macrophages and therefore play a role in the immune response. May also regulate macrophage differentiation into proatherogenic foam cells.1 Publication

Catalytic activityi

Arachidonate + O2 = (5Z,8Z,11Z,13E)-(15S)-15-hydroperoxyicosa-5,8,11,13-tetraenoate.1 Publication
Linoleate + O2 = (9Z,11E)-13-hydroxyoctadeca-9,11-dienoate.1 Publication

Cofactori

Fe cationPROSITE-ProRule annotationNote: Binds 1 Fe cation per subunit.PROSITE-ProRule annotation

Pathwayi: hydroperoxy eicosatetraenoic acid biosynthesis

This protein is involved in the pathway hydroperoxy eicosatetraenoic acid biosynthesis, which is part of Lipid metabolism.
View all proteins of this organism that are known to be involved in the pathway hydroperoxy eicosatetraenoic acid biosynthesis and in Lipid metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi15Calcium 1; via carbonyl oxygenBy similarity1
Metal bindingi17Calcium 1; via carbonyl oxygenBy similarity1
Metal bindingi39Calcium 2By similarity1
Metal bindingi40Calcium 2; via carbonyl oxygenBy similarity1
Metal bindingi42Calcium 2; via carbonyl oxygenBy similarity1
Metal bindingi44Calcium 2By similarity1
Metal bindingi86Calcium 1By similarity1
Metal bindingi87Calcium 1; via carbonyl oxygenBy similarity1
Metal bindingi374Iron; catalyticPROSITE-ProRule annotation1
Metal bindingi379Iron; catalyticPROSITE-ProRule annotation1
Metal bindingi554Iron; catalyticPROSITE-ProRule annotation1
Metal bindingi677Iron; via carboxylate; catalyticPROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

  • arachidonic acid metabolic process Source: UniProtKB
  • hepoxilin biosynthetic process Source: UniProtKB
  • lipoxygenase pathway Source: UniProtKB

Keywordsi

Molecular functionDioxygenase, Oxidoreductase
Biological processLipid metabolism
LigandCalcium, Iron, Lipid-binding, Metal-binding

Enzyme and pathway databases

BRENDAi1.13.11.33 5301
UniPathwayiUPA00881

Names & Taxonomyi

Protein namesi
Recommended name:
Arachidonate 15-lipoxygenase B (EC:1.13.11.33)
Short name:
15-LOX-B
Alternative name(s):
15-lipoxygenase 2
Short name:
15-LOX-2
Arachidonate 15-lipoxygenase type II
Linoleate 13-lipoxygenase 15-LOb (EC:1.13.11.-)
Gene namesi
Name:Alox15b
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi628809 Alox15b

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Cytoskeleton, Membrane, Nucleus

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL3289

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002207021 – 677Arachidonate 15-lipoxygenase BAdd BLAST677

Proteomic databases

PaxDbiQ8K4F2
PRIDEiQ8K4F2

PTM databases

PhosphoSitePlusiQ8K4F2

Interactioni

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000010416

Chemistry databases

BindingDBiQ8K4F2

Structurei

3D structure databases

ProteinModelPortaliQ8K4F2
SMRiQ8K4F2
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini2 – 125PLATPROSITE-ProRule annotationAdd BLAST124
Domaini126 – 677LipoxygenasePROSITE-ProRule annotationAdd BLAST552

Domaini

The PLAT domain can bind calcium ions; this promotes association with membranes.By similarity

Sequence similaritiesi

Belongs to the lipoxygenase family.Curated

Phylogenomic databases

eggNOGiENOG410IF0U Eukaryota
ENOG410YN4N LUCA
HOGENOMiHOG000234358
HOVERGENiHBG005150
InParanoidiQ8K4F2
KOiK08022
PhylomeDBiQ8K4F2

Family and domain databases

InterProiView protein in InterPro
IPR000907 LipOase
IPR013819 LipOase_C
IPR036226 LipOase_C_sf
IPR020834 LipOase_CS
IPR020833 LipOase_Fe_BS
IPR001885 LipOase_mml
IPR001024 PLAT/LH2_dom
IPR036392 PLAT/LH2_dom_sf
PANTHERiPTHR11771 PTHR11771, 1 hit
PfamiView protein in Pfam
PF00305 Lipoxygenase, 1 hit
PF01477 PLAT, 1 hit
PRINTSiPR00087 LIPOXYGENASE
PR00467 MAMLPOXGNASE
SMARTiView protein in SMART
SM00308 LH2, 1 hit
SUPFAMiSSF48484 SSF48484, 1 hit
SSF49723 SSF49723, 1 hit
PROSITEiView protein in PROSITE
PS00711 LIPOXYGENASE_1, 1 hit
PS00081 LIPOXYGENASE_2, 1 hit
PS51393 LIPOXYGENASE_3, 1 hit
PS50095 PLAT, 1 hit

Sequencei

Sequence statusi: Complete.

Q8K4F2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAKFRVRVST GEACGAGTWD KVSVSIVGTH GESPLVPLDH LGKEFSAGAE
60 70 80 90 100
EDFEVTLPQD VGTVLMLRIH KAPPEAPLPL LSFPPDAWYC RWFELEWLPG
110 120 130 140 150
AALRFPCYQW LEGAGELVLR EGAAKVSWQD HHRTLQDQRQ KELESRKDMY
160 170 180 190 200
SWKTYIEGWP HCLDHETVKD LDLNIKYSAM KNAKFFFKAQ SAFTELKFKG
210 220 230 240 250
LLDRTGLWRS LREMKRMFNF HNTPAAEYVF AHWQEDAFFA SQFLNGLNPV
260 270 280 290 300
LIRRCRRLPE NFPVTDEMVA PVLGPGTSLQ AELEKGSLFL VDHGILSGVQ
310 320 330 340 350
TNVINGKPQF SAAPMTLLYQ SPGSGPLLPI AIQLKQTPGP DNPIFLPSDD
360 370 380 390 400
KWDWLLAKTW VRNAEFSIHE ALTHLLHAHL IPEVFALATL RQLPHCHPLF
410 420 430 440 450
KLLIPHTRYT LHINTLAREL LIAPGKVVDK STGLGIGGFS DLIKRNMEQL
460 470 480 490 500
SYSVLCLPED IRARDVGDLP GYYYRDDGMQ IWSAIRSFVS EIVDIYYPSD
510 520 530 540 550
ASVRDDQELQ AWVGEIFSEG FLSQESSGMP SLLDTQEALV QYVTMVIFTC
560 570 580 590 600
SAKHAAVSAS QFDSCVWMPN LPPSMQLPPP TSKGQASPEG FIATLPAVNA
610 620 630 640 650
TCDVIIALWL LSKEPGDRRP LGHYPDEHFT EEVPRRSIAA FQRKLIQISS
660 670
GIRKRNQSLA LPYTYLDPPL IENSVSI
Length:677
Mass (Da):76,145
Last modified:October 1, 2002 - v1
Checksum:i49A47B47C491B8B1
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF415240 mRNA Translation: AAN03708.1
RefSeqiNP_695213.1, NM_153301.2
UniGeneiRn.86437

Genome annotation databases

GeneIDi266604
KEGGirno:266604
UCSCiRGD:628809 rat

Similar proteinsi

Entry informationi

Entry nameiLX15B_RAT
AccessioniPrimary (citable) accession number: Q8K4F2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 1, 2005
Last sequence update: October 1, 2002
Last modified: May 23, 2018
This is version 101 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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