Reviewed, UniProtKB/Swiss-Prot Q8K4F2 (LX15B_RAT)

Names and origin

Protein names

Recommended name:
    Arachidonate 15-lipoxygenase type II
      Short name=15-LOX-2
    EC=1.13.11.33

Gene names

Name:

Alox15b

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	677 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	Arachidonate + O2 = (5Z,8Z,11Z,13E)-(15S)-15-hydroperoxyicosa-5,8,11,13-tetraenoate.
Cofactor	Binds 1 iron ion per subunit By similarity.
Pathway	Lipid metabolism; leukotriene D4 biosynthesis.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the lipoxygenase family. Contains 1 lipoxygenase domain. Contains 1 PLAT domain.

Ontologies

Keywords
Biological process	Leukotriene biosynthesis
Cellular component	Cytoplasm
Ligand	Iron Metal-binding
Molecular function	Dioxygenase Oxidoreductase
Gene Ontology (GO)
Biological process	leukotriene biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW negative regulation of cell cycle Inferred from sequence or structural similarity. Source: UniProtKB negative regulation of cell proliferation Inferred from sequence or structural similarity. Source: UniProtKB negative regulation of growth Inferred from sequence or structural similarity. Source: UniProtKB oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	arachidonate 15-lipoxygenase activity Inferred from sequence or structural similarity. Source: UniProtKB iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW lipoxygenase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 677	677	Arachidonate 15-lipoxygenase type II		PRO_0000220702
Regions
	Domain	2 – 125	124	PLAT
	Domain	126 – 677	552	Lipoxygenase
Sites
	Metal binding	374	1	Iron; catalytic By similarity
	Metal binding	379	1	Iron; catalytic By similarity
	Metal binding	554	1	Iron; catalytic By similarity
	Metal binding	677	1	Iron; via carboxylate; catalytic By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8K4F2-1 [UniParc]. Last modified October 1, 2002. Version 1. Checksum: 49A47B47C491B8B1	FASTA	677	76,145
	10 20 30 40 50 60 MAKFRVRVST GEACGAGTWD KVSVSIVGTH GESPLVPLDH LGKEFSAGAE EDFEVTLPQD 70 80 90 100 110 120 VGTVLMLRIH KAPPEAPLPL LSFPPDAWYC RWFELEWLPG AALRFPCYQW LEGAGELVLR 130 140 150 160 170 180 EGAAKVSWQD HHRTLQDQRQ KELESRKDMY SWKTYIEGWP HCLDHETVKD LDLNIKYSAM 190 200 210 220 230 240 KNAKFFFKAQ SAFTELKFKG LLDRTGLWRS LREMKRMFNF HNTPAAEYVF AHWQEDAFFA 250 260 270 280 290 300 SQFLNGLNPV LIRRCRRLPE NFPVTDEMVA PVLGPGTSLQ AELEKGSLFL VDHGILSGVQ 310 320 330 340 350 360 TNVINGKPQF SAAPMTLLYQ SPGSGPLLPI AIQLKQTPGP DNPIFLPSDD KWDWLLAKTW 370 380 390 400 410 420 VRNAEFSIHE ALTHLLHAHL IPEVFALATL RQLPHCHPLF KLLIPHTRYT LHINTLAREL 430 440 450 460 470 480 LIAPGKVVDK STGLGIGGFS DLIKRNMEQL SYSVLCLPED IRARDVGDLP GYYYRDDGMQ 490 500 510 520 530 540 IWSAIRSFVS EIVDIYYPSD ASVRDDQELQ AWVGEIFSEG FLSQESSGMP SLLDTQEALV 550 560 570 580 590 600 QYVTMVIFTC SAKHAAVSAS QFDSCVWMPN LPPSMQLPPP TSKGQASPEG FIATLPAVNA 610 620 630 640 650 660 TCDVIIALWL LSKEPGDRRP LGHYPDEHFT EEVPRRSIAA FQRKLIQISS GIRKRNQSLA 670 LPYTYLDPPL IENSVSI

References

[1]	"A 15-lipoxygenase in the rat, homolog of human 15-lipoxygenase-2 and mouse 8-lipoxygenase." Boeglin W.E., Schneider C., Brash A.R. Submitted (AUG-2001) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF415240 mRNA. Translation: AAN03708.1.
IPI	IPI00325845.
RefSeq	NP_695213.1.
UniGene	Rn.86437
3D structure databases
HSSP	HSSP built from PDB template 1LOX based on UniProtKB P12530.
ModBase	Search...
Genome annotation databases
Ensembl	ENSRNOG00000007778. Rattus norvegicus. [Contig view]
GeneID	266604.
KEGG	rno:266604.
Organism-specific databases
RGD	628809. Alox15b.
Phylogenomic databases
HOVERGEN	Q8K4F2.
Enzyme and pathway databases
BRENDA	1.13.11.33. 248.
Family and domain databases
InterPro	IPR000907. LipOase. IPR013819. LipOase_C. IPR001024. LipOase_LH2. IPR001885. LipOase_mml. [Graphical view]
Gene3D	G3DSA:2.60.60.20. Lipase_LipOase. 1 hit.
PANTHER	PTHR11771. LipOase. 1 hit.
Pfam	PF00305. Lipoxygenase. 1 hit. PF01477. PLAT. 1 hit. [Graphical view]
PRINTS	PR00087. LIPOXYGENASE. PR00467. MAMLPOXGNASE.
SMART	SM00308. LH2. 1 hit. [Graphical view]
PROSITE	PS00711. LIPOXYGENASE_1. 1 hit. PS00081. LIPOXYGENASE_2. 1 hit. PS51393. LIPOXYGENASE_3. 1 hit. PS50095. PLAT. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	624420.

Entry information

Entry name

LX15B_RAT

Accession

Primary (citable) accession number: Q8K4F2

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 1, 2005
Last sequence update:	October 1, 2002
Last modified:	June 16, 2009
This is version 50 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)