Reviewed, UniProtKB/Swiss-Prot Q8K4D8 (AL1A3_RAT)

Names and origin

Protein names

Recommended name:
    Aldehyde dehydrogenase family 1 member A3
    EC=1.2.1.5
Alternative name(s):
    Aldehyde dehydrogenase 6
    Retinaldehyde dehydrogenase 3
      Short name=RALDH-3
      Short name=RalDH3

Gene names

Name:	Aldh1a3
Synonyms:	Aldh6, Raldh3

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	512 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Recognizes as substrates free retinal and cellular retinol-binding protein-bound retinal. Seems to be the key enzyme in the formation of an RA gradient along the dorso-ventral axis during the early eye development and also in the development of the olfactory system By similarity.
Catalytic activity	An aldehyde + NAD(P)+ + H2O = an acid + NAD(P)H.
Pathway	Cofactor metabolism; retinol metabolism.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the aldehyde dehydrogenase family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	NAD
Molecular function	Oxidoreductase
PTM	Acetylation
Gene Ontology (GO)
Biological process	kidney development Inferred from expression pattern. Source: RGD oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW pituitary gland development Inferred from expression pattern. Source: RGD response to drug Inferred from expression pattern. Source: RGD
Cellular component	cytosol Non-traceable author statement. Source: RGD
Molecular function	aldehyde dehydrogenase [NAD(P)+] activity Inferred from direct assay. Source: RGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed By similarity
	Chain	2 – 512	511	Aldehyde dehydrogenase family 1 member A3		PRO_0000056480
Regions
	Nucleotide binding	257 – 262	6	NAD By similarity
Sites
	Active site	280	1	Proton acceptor By similarity
	Active site	314	1	Nucleophile By similarity
	Site	181	1	Transition state stabilizer By similarity
Amino acid modifications
	Modified residue	2	1	N-acetylalanine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8K4D8-1 [UniParc]. Last modified October 1, 2002. Version 1. Checksum: 2A818D7472F67A5E	FASTA	512	56,171
	10 20 30 40 50 60 MATANGAVEN GQPDGKPPAL PRPIRNLEVK FTKIFINNDW HEPKSGRKFA TYNPSTLEKI 70 80 90 100 110 120 CEVEEGDKPD VDKAVEAAQA AFQRGSPWRR LDALSRGQLL HQLADLIERD RAILATLETM 130 140 150 160 170 180 DTGKPFLHAF FVDLEGCIKT FRYFAGWADK IQGRTIPTDD NVMCFTRHEP IGVCGAITPW 190 200 210 220 230 240 NFPLLMLAWK LAPALCCGNT VVLKPAEQTP LTALYLASLI KEVGFPPGVV NIVPGFGPTV 250 260 270 280 290 300 GAAISSHPQI NKIAFTGSTE VGKLVKEAAS RSNLKRVTLE LGGRNPCIVC ADADLDLAVE 310 320 330 340 350 360 CAHQGVFFNQ GQCCTAASRV FVEEQVYGEF VRRSVEFAKK RPVGDPFDAK TEQGPQIDQK 370 380 390 400 410 420 QFDKILELIE SGKKEGAKLE CGGSAMEDRG LFIKPTVFSD VTDNMRIAKE EIFGPVQPIL 430 440 450 460 470 480 KFKNLEEVIK RANSTDYGLT AAVFTKNLDK ALKLASALES GTVWVNCYNA FYAQAPFGGF 490 500 510 KMSGNGRELG EYALAEYTEV KTVTIKLDEK NP

References

[1]	"A novel short-chain alcohol dehydrogenase from rats with retinol dehydrogenase activity, cyclically expressed in uterine epithelium." Rexer B.N., Ong D.E. Biol. Reprod. 67:1555-1564(2002) [PubMed: 12390888] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: Sprague-Dawley.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF434845 mRNA. Translation: AAN03711.1.
IPI	IPI00203138.
RefSeq	NP_695212.1.
UniGene	Rn.8297
3D structure databases
SMR	Q8K4D8. Positions 20-511.
ModBase	Search...
Protein-protein interaction databases
STRING	Q8K4D8.
Genome annotation databases
Ensembl	ENSRNOT00000043019; ENSRNOP00000045261; ENSRNOG00000013028; Rattus norvegicus. [Genome view]
GeneID	266603.
KEGG	rno:266603.
Organism-specific databases
CTD	266603.
RGD	628662. Aldh1a3.
Phylogenomic databases
eggNOG	maNOG06232.
HOVERGEN	Q8K4D8.
InParanoid	Q8K4D8.
PhylomeDB	Q8K4D8.
Enzyme and pathway databases
BRENDA	1.2.1.36. 248. 1.2.1.5. 248.
Gene expression databases
Genevestigator	Q8K4D8.
GermOnline	ENSRNOG00000013028. Rattus norvegicus.
Family and domain databases
InterPro	IPR016161. Ald_DH/histidinol_DH. IPR016160. Ald_DH_CS. IPR016162. Ald_DH_N. IPR015590. Aldehyde_DH. [Graphical view]
Gene3D	G3DSA:3.40.605.10. Aldehyde_dehydrogenase_N. 1 hit.
PANTHER	PTHR11699. Aldehyde_dehyd. 1 hit.
Pfam	PF00171. Aldedh. 1 hit. [Graphical view]
PROSITE	PS00070. ALDEHYDE_DEHYDR_CYS. 1 hit. PS00687. ALDEHYDE_DEHYDR_GLU. False negative. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	624416.

Entry information

Entry name

AL1A3_RAT

Accession

Primary (citable) accession number: Q8K4D8

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 27, 2004
Last sequence update:	October 1, 2002
Last modified:	January 19, 2010
This is version 54 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)