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Q8K4D3 (S36A1_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 85. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Proton-coupled amino acid transporter 1

Short name=Proton/amino acid transporter 1
Alternative name(s):
Solute carrier family 36 member 1
Gene names
Name:Slc36a1
Synonyms:Pat1
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length475 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Neutral amino acid/proton symporter. Has a pH-dependent electrogenic transport activity for small amino acids such as glycine, alanine and proline. Besides small apolar L-amino acids, it also recognize their D-enantiomers and selected amino acid derivatives such as gamma-aminobutyric acid. Ref.1

Subcellular location

Cell membrane; Multi-pass membrane protein By similarity. Lysosome membrane; Multi-pass membrane protein By similarity.

Tissue specificity

Highly expressed in small intestine, colon, kidney and brain. Ref.1

Sequence similarities

Belongs to the amino acid/polyamine transporter 2 family.

Ontologies

Keywords
   Biological processAmino-acid transport
Symport
Transport
   Cellular componentCell membrane
Lysosome
Membrane
   DomainTransmembrane
Transmembrane helix
   PTMDisulfide bond
Glycoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processL-alanine transport

Inferred from direct assay Ref.1. Source: MGI

glycine transport

Inferred from direct assay Ref.1. Source: MGI

proline transmembrane transport

Inferred from direct assay Ref.1. Source: GOC

proline transport

Inferred from direct assay Ref.1. Source: MGI

proton transport

Inferred from direct assay Ref.1. Source: MGI

   Cellular_componentendoplasmic reticulum

Inferred from electronic annotation. Source: Ensembl

integral component of membrane

Inferred from electronic annotation. Source: UniProtKB-KW

lysosomal membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

lysosome

Inferred from sequence alignment Ref.1. Source: MGI

plasma membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionL-alanine transmembrane transporter activity

Inferred from direct assay Ref.1. Source: MGI

L-proline transmembrane transporter activity

Inferred from direct assay Ref.1. Source: MGI

glycine transmembrane transporter activity

Inferred from direct assay Ref.1. Source: MGI

hydrogen ion transmembrane transporter activity

Inferred from electronic annotation. Source: Ensembl

symporter activity

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 475475Proton-coupled amino acid transporter 1
PRO_0000093826

Regions

Topological domain1 – 5050Cytoplasmic Potential
Transmembrane51 – 7121Helical; Potential
Topological domain72 – 776Extracellular Potential
Transmembrane78 – 9821Helical; Potential
Topological domain99 – 14042Cytoplasmic Potential
Transmembrane141 – 16121Helical; Potential
Topological domain162 – 18928Extracellular Potential
Transmembrane190 – 21021Helical; Potential
Topological domain211 – 2144Cytoplasmic Potential
Transmembrane215 – 23521Helical; Potential
Topological domain236 – 25621Extracellular Potential
Transmembrane257 – 27721Helical; Potential
Topological domain278 – 28811Cytoplasmic Potential
Transmembrane289 – 30921Helical; Potential
Topological domain310 – 34132Extracellular Potential
Transmembrane342 – 36221Helical; Potential
Topological domain363 – 3719Cytoplasmic Potential
Transmembrane372 – 39221Helical; Potential
Topological domain393 – 3964Extracellular Potential
Transmembrane397 – 41721Helical; Potential
Topological domain418 – 43821Cytoplasmic Potential
Transmembrane439 – 45921Helical; Potential
Topological domain460 – 47516Extracellular Potential

Amino acid modifications

Glycosylation1731N-linked (GlcNAc...) Potential
Glycosylation1821N-linked (GlcNAc...) Potential
Glycosylation4691N-linked (GlcNAc...) Potential
Disulfide bond179 ↔ 328 By similarity

Experimental info

Sequence conflict431N → S in AAO37090. Ref.2
Sequence conflict1981L → P in AAO37090. Ref.2

Sequences

Sequence LengthMass (Da)Tools
Q8K4D3 [UniParc].

Last modified October 1, 2002. Version 1.
Checksum: 113C23309F2B51F1

FASTA47552,466
        10         20         30         40         50         60 
MSTQRLRNED YHDYSSTDVS PEESPSEGLG SFSPGSYQRL GENSSMTWFQ TLIHLLKGNI 

        70         80         90        100        110        120 
GTGLLGLPLA VKNAGLLLGP LSLLVIGIVA VHCMGILVKC AHHLCRRLNK PFLDYGDTVM 

       130        140        150        160        170        180 
YGLECSPSTW VRNHSHWGRR IVDFFLIVTQ LGFCCVYFVF LADNFKQVIE AANGTTTNCN 

       190        200        210        220        230        240 
NNVTVIPTPT MDSRLYMLSF LPFLVLLSFI RNLRVLSIFS LLANISMFVS LIMIYQFIVQ 

       250        260        270        280        290        300 
RIPDPSHLPL VAPWKTYPLF FGTAIFAFEG IGVVLPLENK MKDSQKFPLI LYLGMAIITV 

       310        320        330        340        350        360 
LYISLGSLGY LQFGANIKGS ITLNLPNCWL YQSVKLLYSI GIFFTYALQF YVAAEIIIPA 

       370        380        390        400        410        420 
IVSRVPEHFE LMVDLCVRTA MVCVTCVLAI LIPRLDLVIS LVGSVSSSAL ALIIPPLLEV 

       430        440        450        460        470 
VTYYGEGISP LTVTKDALIS ILGFVGFVVG TYESLCELIQ PSHSDSSTNS TSAFI 

« Hide

References

[1]"Functional characterization of two novel mammalian electrogenic proton-dependent amino acid cotransporters."
Boll M., Foltz M., Rubio-Aliaga I., Kottra G., Daniel H.
J. Biol. Chem. 277:22966-22973(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY.
Strain: C57BL/6.
[2]"Organization and expression of the SLC36 cluster of amino acid transporter genes."
Bermingham J.R. Jr., Pennington J.
Mamm. Genome 15:114-125(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF453743 mRNA. Translation: AAM80480.1.
AY211262 mRNA. Translation: AAO37090.1.
CCDSCCDS36156.1.
RefSeqNP_694779.3. NM_153139.4.
UniGeneMm.35732.

3D structure databases

ProteinModelPortalQ8K4D3.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActQ8K4D3. 1 interaction.

Protein family/group databases

TCDB2.A.18.8.1. the amino acid/auxin permease (aaap) family.

PTM databases

PhosphoSiteQ8K4D3.

Proteomic databases

PaxDbQ8K4D3.
PRIDEQ8K4D3.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000020499; ENSMUSP00000020499; ENSMUSG00000020261.
ENSMUST00000108867; ENSMUSP00000104495; ENSMUSG00000020261.
ENSMUST00000108872; ENSMUSP00000104500; ENSMUSG00000020261.
GeneID215335.
KEGGmmu:215335.
UCSCuc007izd.1. mouse.

Organism-specific databases

CTD206358.
MGIMGI:2445299. Slc36a1.

Phylogenomic databases

eggNOGCOG0814.
GeneTreeENSGT00390000011245.
HOGENOMHOG000006823.
HOVERGENHBG057183.
InParanoidQ8K4D3.
KOK14209.
OMADCHNNET.
OrthoDBEOG73V6KC.
PhylomeDBQ8K4D3.
TreeFamTF314873.

Gene expression databases

ArrayExpressQ8K4D3.
BgeeQ8K4D3.
GenevestigatorQ8K4D3.

Family and domain databases

InterProIPR013057. AA_transpt_TM.
[Graphical view]
PfamPF01490. Aa_trans. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSSLC36A1. mouse.
NextBio374674.
PROQ8K4D3.
SOURCESearch...

Entry information

Entry nameS36A1_MOUSE
AccessionPrimary (citable) accession number: Q8K4D3
Secondary accession number(s): Q811N9
Entry history
Integrated into UniProtKB/Swiss-Prot: August 16, 2004
Last sequence update: October 1, 2002
Last modified: July 9, 2014
This is version 85 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot