Reviewed, UniProtKB/Swiss-Prot Q8K4B7 (FMO4_RAT)

Names and origin

Protein names

Recommended name:
    Dimethylaniline monooxygenase [N-oxide-forming] 4
    EC=1.14.13.8
Alternative name(s):
    Hepatic flavin-containing monooxygenase 4
      Short name=FMO 4
    Dimethylaniline oxidase 4

Gene names

Name:

Fmo4

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	560 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	This protein is involved in the oxidative metabolism of a variety of xenobiotics such as drugs and pesticides By similarity.
Catalytic activity	N,N-dimethylaniline + NADPH + O2 = N,N-dimethylaniline N-oxide + NADP+ + H2O.
Cofactor	FAD By similarity.
Subcellular location	Microsome membrane By similarity. Endoplasmic reticulum membrane By similarity.
Sequence similarities	Belongs to the FMO family.

Ontologies

Keywords
Cellular component	Endoplasmic reticulum Membrane Microsome
Domain	Transmembrane
Ligand	FAD Flavoprotein NADP
Molecular function	Monooxygenase Oxidoreductase
PTM	Acetylation
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW intrinsic to endoplasmic reticulum membrane Inferred from electronic annotation. Source: InterPro microsome Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	FAD binding Inferred from electronic annotation. Source: InterPro NADP or NADPH binding Inferred from electronic annotation. Source: InterPro flavin-containing monooxygenase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed By similarity
	Chain	2 – 560	559	Dimethylaniline monooxygenase [N-oxide-forming] 4		PRO_0000147663
Regions
	Transmembrane	516 – 530	15	Potential
	Nucleotide binding	9 – 14	6	FAD Potential
	Nucleotide binding	191 – 196	6	NADP Potential
Amino acid modifications
	Modified residue	2	1	N-acetylalanine By similarity
Experimental info
	Sequence conflict	244	1	Q → R in AAM46764. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q8K4B7-1 [UniParc]. Last modified January 23, 2007. Version 3. Checksum: 9A7A77C41A328EFB	FASTA	560	63,660
	10 20 30 40 50 60 MAKKVAVIGA GVSGLSSIKC CLDENLEPTC FERSSDFGGL WKFAEASEDG MTRVYRSLVT 70 80 90 100 110 120 NVCKEMSCYS DFPFHEDYPN FMSHEKFWDY LREFAEHFGL LKYIRFKTTV RSVTKRPDFS 130 140 150 160 170 180 ETGQWEVVTE TEGKQDRAVF DAVMVCTGQF LSPRLPLESF PGIHKFKGQI LHSQEYRIPD 190 200 210 220 230 240 AFRGKRILVV GLGNTGGDVA VELSGIAAQV FLSTRTGAWV RSRSSVGGYP LNMMQTRWRN 250 260 270 280 290 300 FLAQVLPSRF VSWNQERQMN KIFNHENYGL SIAKGKKPKF IVNDELPTCI LCGKITMKTS 310 320 330 340 350 360 VKDFTESSIV FEDGTIEANI DVVIFTTGYE FSFPFFEEPL KSLCTKKVIL YKRVFPPNLE 370 380 390 400 410 420 RSTLAIIGLI SLTGSILVGT EFQARWATRV FKGLCNIPPS QKLMAEAIKK EELIKRGVIK 430 440 450 460 470 480 DTSQDKLDFI SYMDELTQCI GAKPNIPLLF LKDPRLAWEV FFGPCTPYQY RLMGPGRWDG 490 500 510 520 530 540 ARNAILTQWD RTVKPLKTRT VPKSQEPASL SRYLKTWGAP VLIVSLLLIY KSSLFLELVQ 550 560 SKLQGRFSPS RILWYIPQNS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning, sequencing and tissue distribution of rat flavin-containing monooxygenase 4. Two different FMO4 are produced by tissue specific alternative splicing." Lattard V., Longin-Sauvageon C., Benoit E. Submitted (DEC-2001) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: Sprague-Dawley.
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Kidney.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF458416 mRNA. Translation: AAM46764.1. BC081721 mRNA. Translation: AAH81721.1.
IPI	IPI00325823.
RefSeq	NP_653147.1.
UniGene	Rn.216582
3D structure databases
SMR	Q8K4B7. Positions 3-447.
ModBase	Search...
Protein-protein interaction databases
STRING	Q8K4B7.
Genome annotation databases
Ensembl	ENSRNOT00000004599; ENSRNOP00000004599; ENSRNOG00000003400; Rattus norvegicus. [Genome view]
GeneID	246247.
KEGG	rno:246247.
UCSC	NM_144561. rat.
Organism-specific databases
CTD	246247.
RGD	628601. Fmo4.
Phylogenomic databases
eggNOG	maNOG04376.
HOVERGEN	Q8K4B7.
InParanoid	Q8K4B7.
OMA	PLKSLCT.
OrthoDB	EOG9GMXG7.
PhylomeDB	Q8K4B7.
Enzyme and pathway databases
BRENDA	1.14.13.8. 248.
Gene expression databases
ArrayExpress	Q8K4B7.
Genevestigator	Q8K4B7.
GermOnline	ENSRNOG00000003400. Rattus norvegicus.
Family and domain databases
InterPro	IPR012143. dManiline_mOase. IPR000960. Flavin_mOase. IPR020946. Flavin_mOase-like. IPR002256. Flavin_mOase_4. [Graphical view]
Pfam	PF00743. FMO-like. 1 hit. [Graphical view]
PIRSF	PIRSF000332. FMO. 1 hit.
PRINTS	PR00370. FMOXYGENASE. PR01124. FMOXYGENASE4.
ProtoNet	Search...
Other Resources
NextBio	623572.

Entry information

Entry name

FMO4_RAT

Accession

Primary (citable) accession number: Q8K4B7
Secondary accession number(s): Q66HR6

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 25, 2005
Last sequence update:	January 23, 2007
Last modified:	January 19, 2010
This is version 59 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)