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Q8K4B2

- IRAK3_MOUSE

UniProt

Q8K4B2 - IRAK3_MOUSE

Protein

Interleukin-1 receptor-associated kinase 3

Gene

Irak3

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 100 (01 Oct 2014)
      Sequence version 2 (25 Oct 2004)
      Previous versions | rss
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    Functioni

    Inhibits dissociation of IRAK1 and IRAK4 from the Toll-like receptor signaling complex by either inhibiting the phosphorylation of IRAK1 and IRAK4 or stabilizing the receptor complex.2 Publications1 Publication

    Catalytic activityi

    ATP + a protein = ADP + a phosphoprotein.1 Publication

    Cofactori

    Magnesium.1 Publication

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei205 – 2051ATPBy similarityPROSITE-ProRule annotation
    Binding sitei324 – 3241ATPPROSITE-ProRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi184 – 1929ATPBy similarityPROSITE-ProRule annotation
    Nucleotide bindingi308 – 3114ATPPROSITE-ProRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB
    2. magnesium ion binding Source: UniProtKB
    3. protein binding Source: IntAct
    4. protein kinase activity Source: MGI
    5. protein serine/threonine kinase activity Source: UniProtKB

    GO - Biological processi

    1. cytokine-mediated signaling pathway Source: UniProtKB
    2. interleukin-1-mediated signaling pathway Source: MGI
    3. negative regulation of cytokine-mediated signaling pathway Source: BHF-UCL
    4. negative regulation of innate immune response Source: BHF-UCL
    5. negative regulation of interleukin-12 production Source: BHF-UCL
    6. negative regulation of interleukin-6 production Source: BHF-UCL
    7. negative regulation of macrophage cytokine production Source: BHF-UCL
    8. negative regulation of MAP kinase activity Source: BHF-UCL
    9. negative regulation of NF-kappaB transcription factor activity Source: Ensembl
    10. negative regulation of protein catabolic process Source: BHF-UCL
    11. negative regulation of protein complex disassembly Source: Ensembl
    12. negative regulation of toll-like receptor signaling pathway Source: BHF-UCL
    13. negative regulation of tumor necrosis factor production Source: BHF-UCL
    14. positive regulation of macrophage tolerance induction Source: BHF-UCL
    15. positive regulation of NF-kappaB transcription factor activity Source: MGI
    16. protein autophosphorylation Source: MGI
    17. protein phosphorylation Source: UniProtKB
    18. regulation of protein complex disassembly Source: UniProtKB
    19. response to exogenous dsRNA Source: BHF-UCL
    20. response to lipopolysaccharide Source: BHF-UCL
    21. response to peptidoglycan Source: BHF-UCL
    22. response to virus Source: BHF-UCL

    Keywords - Molecular functioni

    Kinase, Serine/threonine-protein kinase, Transferase

    Keywords - Ligandi

    ATP-binding, Magnesium, Nucleotide-binding

    Enzyme and pathway databases

    ReactomeiREACT_224208. Interleukin-1 signaling.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Interleukin-1 receptor-associated kinase 3 (EC:2.7.11.1)
    Short name:
    IRAK-3
    Alternative name(s):
    IL-1 receptor-associated kinase M
    Short name:
    IRAK-M
    Gene namesi
    Name:Irak3Imported
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 10

    Organism-specific databases

    MGIiMGI:1921164. Irak3.

    Subcellular locationi

    GO - Cellular componenti

    1. cytoplasm Source: BHF-UCL
    2. nucleus Source: BHF-UCL

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 609609Interleukin-1 receptor-associated kinase 3PRO_0000086034Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei480 – 4801PhosphoserineBy similarity

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    PRIDEiQ8K4B2.

    PTM databases

    PhosphoSiteiQ8K4B2.

    Expressioni

    Tissue specificityi

    Highly expressed in liver and thymus and at lower levels in heart, brain, spleen and kidney.1 Publication

    Gene expression databases

    ArrayExpressiQ8K4B2.
    BgeeiQ8K4B2.
    CleanExiMM_IRAK3.
    GenevestigatoriQ8K4B2.

    Interactioni

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    IRAK4Q9NWZ34EBI-646179,EBI-448378From a different organism.
    Irak4Q8R4K27EBI-646179,EBI-3842721
    Traf6P701963EBI-646179,EBI-448028

    Protein-protein interaction databases

    BioGridi216351. 2 interactions.
    IntActiQ8K4B2. 6 interactions.

    Structurei

    3D structure databases

    ProteinModelPortaliQ8K4B2.
    SMRiQ8K4B2. Positions 184-457.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini41 – 10666DeathPROSITE-ProRule annotationAdd
    BLAST
    Domaini178 – 463286Protein kinasePROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Contains 1 death domain.PROSITE-ProRule annotation
    Contains 1 protein kinase domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG0515.
    GeneTreeiENSGT00530000063073.
    HOGENOMiHOG000060319.
    HOVERGENiHBG052146.
    InParanoidiQ8K4B2.
    KOiK04732.
    OMAiFKQEKKM.
    OrthoDBiEOG7KQ21F.
    PhylomeDBiQ8K4B2.
    TreeFamiTF328924.

    Family and domain databases

    Gene3Di1.10.533.10. 1 hit.
    InterProiIPR011029. DEATH-like_dom.
    IPR000488. Death_domain.
    IPR011009. Kinase-like_dom.
    IPR000719. Prot_kinase_dom.
    [Graphical view]
    PfamiPF00531. Death. 1 hit.
    PF00069. Pkinase. 1 hit.
    [Graphical view]
    SUPFAMiSSF47986. SSF47986. 1 hit.
    SSF56112. SSF56112. 1 hit.
    PROSITEiPS50017. DEATH_DOMAIN. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q8K4B2-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAGRCGARGA LSPQLLLFDL PPALLGELCG ILDSCDGPLG WRGLAERLSN    50
    SWLDVRHIEK YVNQGKSGTR ELLWSWAQKN KTIGDLLEVL QDMGHQRAIH 100
    LIINYGVSWT PSVQTHHELP FPSFPPEVKH ACRENDPGPL EPANVTVDNV 150
    LVPEHNEKGT LQKTPISFQS ILEGTKHFHK DFLIGEGEIF EVYRVDIRNQ 200
    AYAVKLFKQE KKMQLKKHWK RFLSELEVLL LFRHPHILEL AAYFTETEKL 250
    CLVYPYMSNG TLFDRLQCTN GTTPLSWHVR ISVLIGIAKA IQYLHNTQPC 300
    AVICGNVSSA NILLDDQLQP KLTDFAAAHF RPNLEQQSST INMTGGGRKH 350
    LWYMPEEYIR QGRLSVKTDV YSFGIVIMEV LTGCKVVLDD PKHVQLRDLL 400
    MELMEKRGLD SCLSFLDRKI PPCPRNFSAK LFSLAGRCVA TKAKLRPTMD 450
    EVLSSLESTQ PSLYFAEDPP TSLKSFRCPS PLFLDNVPSI PVEDDENQNN 500
    HSVPPKEVLG TDRVTQKTPF ECSQSEVTFL GLDRNRGNRG SEADCNVPSS 550
    SHEECWSPEL VAPSQDLSPT VISLGSSWEV PGHSYGSKPM EKRCSSGLFC 600
    SEHEQSKKQ 609
    Length:609
    Mass (Da):68,455
    Last modified:October 25, 2004 - v2
    Checksum:iA63E010C9F29D856
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti35 – 351C → W in AAM83393. (PubMed:12150927)Curated
    Sequence conflicti42 – 421R → W in AAM83393. (PubMed:12150927)Curated
    Sequence conflicti62 – 621V → L in AAM83393. (PubMed:12150927)Curated
    Sequence conflicti126 – 1261P → L in AAM83393. (PubMed:12150927)Curated
    Sequence conflicti147 – 1471V → M in AAM83393. (PubMed:12150927)Curated
    Sequence conflicti282 – 2821S → G in BAC33612. (PubMed:16141072)Curated
    Sequence conflicti282 – 2821S → N in AAM83393. (PubMed:12150927)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF461763 mRNA. Translation: AAM83393.1.
    AJ440757 mRNA. Translation: CAD29448.2.
    AK029057 mRNA. Translation: BAC26270.1.
    AK049210 mRNA. Translation: BAC33612.1.
    CCDSiCCDS24204.1.
    RefSeqiNP_082955.2. NM_028679.3.
    UniGeneiMm.146194.

    Genome annotation databases

    EnsembliENSMUST00000020448; ENSMUSP00000020448; ENSMUSG00000020227.
    GeneIDi73914.
    KEGGimmu:73914.
    UCSCiuc007het.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF461763 mRNA. Translation: AAM83393.1 .
    AJ440757 mRNA. Translation: CAD29448.2 .
    AK029057 mRNA. Translation: BAC26270.1 .
    AK049210 mRNA. Translation: BAC33612.1 .
    CCDSi CCDS24204.1.
    RefSeqi NP_082955.2. NM_028679.3.
    UniGenei Mm.146194.

    3D structure databases

    ProteinModelPortali Q8K4B2.
    SMRi Q8K4B2. Positions 184-457.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 216351. 2 interactions.
    IntActi Q8K4B2. 6 interactions.

    PTM databases

    PhosphoSitei Q8K4B2.

    Proteomic databases

    PRIDEi Q8K4B2.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000020448 ; ENSMUSP00000020448 ; ENSMUSG00000020227 .
    GeneIDi 73914.
    KEGGi mmu:73914.
    UCSCi uc007het.1. mouse.

    Organism-specific databases

    CTDi 11213.
    MGIi MGI:1921164. Irak3.

    Phylogenomic databases

    eggNOGi COG0515.
    GeneTreei ENSGT00530000063073.
    HOGENOMi HOG000060319.
    HOVERGENi HBG052146.
    InParanoidi Q8K4B2.
    KOi K04732.
    OMAi FKQEKKM.
    OrthoDBi EOG7KQ21F.
    PhylomeDBi Q8K4B2.
    TreeFami TF328924.

    Enzyme and pathway databases

    Reactomei REACT_224208. Interleukin-1 signaling.

    Miscellaneous databases

    NextBioi 339332.
    PROi Q8K4B2.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q8K4B2.
    Bgeei Q8K4B2.
    CleanExi MM_IRAK3.
    Genevestigatori Q8K4B2.

    Family and domain databases

    Gene3Di 1.10.533.10. 1 hit.
    InterProi IPR011029. DEATH-like_dom.
    IPR000488. Death_domain.
    IPR011009. Kinase-like_dom.
    IPR000719. Prot_kinase_dom.
    [Graphical view ]
    Pfami PF00531. Death. 1 hit.
    PF00069. Pkinase. 1 hit.
    [Graphical view ]
    SUPFAMi SSF47986. SSF47986. 1 hit.
    SSF56112. SSF56112. 1 hit.
    PROSITEi PS50017. DEATH_DOMAIN. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "IRAK-M is a negative regulator of Toll-like receptor signaling."
      Kobayashi K., Hernandez L.D., Galan J.E., Janeway C.A. Jr., Medzhitov R., Flavell R.A.
      Cell 110:191-202(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION.
      Strain: C57BL/6Imported.
      Tissue: Macrophage1 Publication.
    2. "Identification and characterization of murine IRAK-M."
      Rosati O., Martin M.U.
      Biochem. Biophys. Res. Commun. 293:1472-1477(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, COFACTOR, TISSUE SPECIFICITY.
      Strain: BALB/cImported.
      Tissue: Macrophage1 Publication.
    3. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6J.
      Tissue: Skin.

    Entry informationi

    Entry nameiIRAK3_MOUSE
    AccessioniPrimary (citable) accession number: Q8K4B2
    Secondary accession number(s): Q8C7U8, Q8CE40, Q8K1S8
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 9, 2004
    Last sequence update: October 25, 2004
    Last modified: October 1, 2014
    This is version 100 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Caution

    Asn-306 is present instead of the conserved Asp which is expected to be an active site residue. Low level autophosphorylation activity has been reported in PubMed:12054681, while other authors describe this as an inactive kinase.Curated

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. Human and mouse protein kinases
      Human and mouse protein kinases: classification and index
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3