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Protein

Membrane frizzled-related protein

Gene

Mfrp

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

May play a role in eye development.

GO - Biological processi

  • eye photoreceptor cell development Source: MGI
  • retina development in camera-type eye Source: MGI
  • visual perception Source: MGI
Complete GO annotation...

Names & Taxonomyi

Protein namesi
Recommended name:
Membrane frizzled-related protein
Alternative name(s):
Membrane-type frizzled-related protein
Gene namesi
Name:Mfrp
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 9

Organism-specific databases

MGIiMGI:2385957. Mfrp.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 6969CytoplasmicSequence analysisAdd
BLAST
Transmembranei70 – 9021Helical; Signal-anchor for type II membrane proteinSequence analysisAdd
BLAST
Topological domaini91 – 584494ExtracellularSequence analysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Involvement in diseasei

Defects in Mfrp are the cause of retinal degeneration 6 (RD6). RD6 is an autosomal recessive degeneration of the photoreceptors causing dysfunction of both rods and cones.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 584584Membrane frizzled-related proteinPRO_0000228133Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi150 ↔ 176By similarity
Disulfide bondi203 ↔ 222By similarity
Glycosylationi233 – 2331N-linked (GlcNAc...)Sequence analysis
Disulfide bondi266 ↔ 278By similarity
Disulfide bondi273 ↔ 291By similarity
Disulfide bondi285 ↔ 300By similarity
Disulfide bondi307 ↔ 333By similarity
Disulfide bondi360 ↔ 383By similarity
Glycosylationi421 – 4211N-linked (GlcNAc...)Sequence analysis
Disulfide bondi433 ↔ 451By similarity
Disulfide bondi445 ↔ 459By similarity
Glycosylationi458 – 4581N-linked (GlcNAc...)Sequence analysis
Disulfide bondi471 ↔ 533By similarity
Disulfide bondi479 ↔ 526By similarity
Disulfide bondi517 ↔ 554By similarity
Disulfide bondi543 ↔ 581By similarity
Disulfide bondi547 ↔ 569By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiQ8K480.
PeptideAtlasiQ8K480.
PRIDEiQ8K480.

PTM databases

iPTMnetiQ8K480.
PhosphoSiteiQ8K480.

Expressioni

Tissue specificityi

Expressed in retinal pigment epithelium and ciliary epithelium of the eye.2 Publications

Gene expression databases

BgeeiENSMUSG00000034739.
CleanExiMM_C1QTNF5.
ExpressionAtlasiQ8K480. baseline.
GenevisibleiQ8K480. MM.

Interactioni

Subunit structurei

Interacts with C1QTNF5.1 Publication

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000034654.

Structurei

3D structure databases

ProteinModelPortaliQ8K480.
SMRiQ8K480. Positions 155-418, 471-573.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini150 – 259110CUB 1PROSITE-ProRule annotationAdd
BLAST
Domaini265 – 30137LDL-receptor class A 1PROSITE-ProRule annotationAdd
BLAST
Domaini307 – 420114CUB 2PROSITE-ProRule annotationAdd
BLAST
Domaini426 – 46035LDL-receptor class A 2PROSITE-ProRule annotationAdd
BLAST
Domaini466 – 584119FZPROSITE-ProRule annotationAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi95 – 14955Thr-richAdd
BLAST

Sequence similaritiesi

Contains 2 CUB domains.PROSITE-ProRule annotation
Contains 1 FZ (frizzled) domain.PROSITE-ProRule annotation
Contains 2 LDL-receptor class A domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3577. Eukaryota.
KOG4292. Eukaryota.
ENOG410XR68. LUCA.
GeneTreeiENSGT00760000119018.
HOGENOMiHOG000048709.
HOVERGENiHBG081968.
InParanoidiQ8K480.
OMAiGLEACAQ.
OrthoDBiEOG091G0CGM.
PhylomeDBiQ8K480.
TreeFamiTF316506.

Family and domain databases

Gene3Di1.10.2000.10. 1 hit.
2.60.120.290. 2 hits.
4.10.400.10. 1 hit.
InterProiIPR000859. CUB_dom.
IPR020067. Frizzled_dom.
IPR023415. LDLR_class-A_CS.
IPR002172. LDrepeatLR_classA_rpt.
[Graphical view]
PfamiPF00431. CUB. 2 hits.
PF01392. Fz. 1 hit.
PF00057. Ldl_recept_a. 1 hit.
[Graphical view]
SMARTiSM00042. CUB. 2 hits.
SM00063. FRI. 1 hit.
SM00192. LDLa. 1 hit.
[Graphical view]
SUPFAMiSSF49854. SSF49854. 2 hits.
SSF57424. SSF57424. 1 hit.
SSF63501. SSF63501. 1 hit.
PROSITEiPS01180. CUB. 2 hits.
PS50038. FZ. 1 hit.
PS01209. LDLRA_1. 1 hit.
PS50068. LDLRA_2. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8K480-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MKDYDDVILR PEASELSKTE FCNPAFDPEA GPSCPPPALQ RDVGSRLQAP
60 70 80 90 100
WHAQRLRGLQ PDCHFSWFCI LLLSGLLLLL LGLLVAVILA QLQATSLPRT
110 120 130 140 150
TKNPLLTRGL TPMGVIPSTT PNTTTTTTTT TPARTGQQEA AMSPTHQTTC
160 170 180 190 200
GGLLPGPSGF FSSPNYPDLY PPLSHCVWHI QVAAGQTIQL KIQALSIESM
210 220 230 240 250
LTCLFDRLEI ISEPTGPLLR VCGKTPPATL NTNTSHLRVS FVSDNDVEGS
260 270 280 290 300
GFQAWYQAVA PGHWSCAHNE FHCDLLLCLK RDSVCDGITE CADGSDEANC
310 320 330 340 350
SAKTLGCGGN LTGLYGVFST PNYPQHYPHQ QLCTWYIEVP VGYGIRLEFH
360 370 380 390 400
NFSLEAQAEC KFDYVEVYEA SNLGTFSFLG RFCGAEPPLN VVSSMHQLAV
410 420 430 440 450
IFKTDLGISS GGFLATYQAI NTTESGCPWA EFCQSGGYRD LQWMCDLWKD
460 470 480 490 500
CANDSNDNCS SHLSPQPDLT CEPVQVEMCL GLSYNTTAFP NIWVGLATQT
510 520 530 540 550
EVTDILRGYK SLTSLPCYQT FQRFLCGLLV PRCTSLGTIL PPCRSVCQAA
560 570 580
EQQCQSSLAL LGTPWPFNCN RLPVAASLEA CSQP
Length:584
Mass (Da):63,669
Last modified:October 1, 2002 - v1
Checksum:i0CF6A6403107E16C
GO
Isoform 2 (identifier: Q8K480-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     425-431: SGCPWAE → K

Note: No experimental confirmation available.
Show »
Length:578
Mass (Da):63,067
Checksum:i8FA0ABDE294DEF88
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti98 – 981P → H in BAC35452 (PubMed:16141072).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei425 – 4317SGCPWAE → K in isoform 2. 1 PublicationVSP_017664

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF469650 mRNA. Translation: AAM89216.1.
AK053629 mRNA. Translation: BAC35452.1.
CCDSiCCDS40597.1. [Q8K480-1]
CCDS52776.1. [Q8K480-2]
RefSeqiNP_001177242.1. NM_001190313.1.
NP_001177243.1. NM_001190314.1. [Q8K480-2]
NP_663588.2. NM_145613.4.
NP_667337.1. NM_147126.3. [Q8K480-1]
UniGeneiMm.227110.

Genome annotation databases

EnsembliENSMUST00000034654; ENSMUSP00000034654; ENSMUSG00000034739. [Q8K480-1]
ENSMUST00000161381; ENSMUSP00000124456; ENSMUSG00000034739. [Q8K480-2]
GeneIDi235312.
259172.
KEGGimmu:259172.
UCSCiuc009pbr.2. mouse. [Q8K480-1]
uc012grq.1. mouse. [Q8K480-2]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF469650 mRNA. Translation: AAM89216.1.
AK053629 mRNA. Translation: BAC35452.1.
CCDSiCCDS40597.1. [Q8K480-1]
CCDS52776.1. [Q8K480-2]
RefSeqiNP_001177242.1. NM_001190313.1.
NP_001177243.1. NM_001190314.1. [Q8K480-2]
NP_663588.2. NM_145613.4.
NP_667337.1. NM_147126.3. [Q8K480-1]
UniGeneiMm.227110.

3D structure databases

ProteinModelPortaliQ8K480.
SMRiQ8K480. Positions 155-418, 471-573.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000034654.

PTM databases

iPTMnetiQ8K480.
PhosphoSiteiQ8K480.

Proteomic databases

PaxDbiQ8K480.
PeptideAtlasiQ8K480.
PRIDEiQ8K480.

Protocols and materials databases

DNASUi259172.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000034654; ENSMUSP00000034654; ENSMUSG00000034739. [Q8K480-1]
ENSMUST00000161381; ENSMUSP00000124456; ENSMUSG00000034739. [Q8K480-2]
GeneIDi235312.
259172.
KEGGimmu:259172.
UCSCiuc009pbr.2. mouse. [Q8K480-1]
uc012grq.1. mouse. [Q8K480-2]

Organism-specific databases

CTDi114902.
83552.
MGIiMGI:2385957. Mfrp.

Phylogenomic databases

eggNOGiKOG3577. Eukaryota.
KOG4292. Eukaryota.
ENOG410XR68. LUCA.
GeneTreeiENSGT00760000119018.
HOGENOMiHOG000048709.
HOVERGENiHBG081968.
InParanoidiQ8K480.
OMAiGLEACAQ.
OrthoDBiEOG091G0CGM.
PhylomeDBiQ8K480.
TreeFamiTF316506.

Miscellaneous databases

PROiQ8K480.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000034739.
CleanExiMM_C1QTNF5.
ExpressionAtlasiQ8K480. baseline.
GenevisibleiQ8K480. MM.

Family and domain databases

Gene3Di1.10.2000.10. 1 hit.
2.60.120.290. 2 hits.
4.10.400.10. 1 hit.
InterProiIPR000859. CUB_dom.
IPR020067. Frizzled_dom.
IPR023415. LDLR_class-A_CS.
IPR002172. LDrepeatLR_classA_rpt.
[Graphical view]
PfamiPF00431. CUB. 2 hits.
PF01392. Fz. 1 hit.
PF00057. Ldl_recept_a. 1 hit.
[Graphical view]
SMARTiSM00042. CUB. 2 hits.
SM00063. FRI. 1 hit.
SM00192. LDLa. 1 hit.
[Graphical view]
SUPFAMiSSF49854. SSF49854. 2 hits.
SSF57424. SSF57424. 1 hit.
SSF63501. SSF63501. 1 hit.
PROSITEiPS01180. CUB. 2 hits.
PS50038. FZ. 1 hit.
PS01209. LDLRA_1. 1 hit.
PS50068. LDLRA_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiMFRP_MOUSE
AccessioniPrimary (citable) accession number: Q8K480
Secondary accession number(s): Q8BPP4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 21, 2006
Last sequence update: October 1, 2002
Last modified: September 7, 2016
This is version 105 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.