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Protein

Proton-coupled amino acid transporter 2

Gene

Slc36a2

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in a pH-dependent electrogenic neuronal transport and sequestration of small amino acids amino acids such as glycine, alanine and proline. Inhibited by sarcosine. Inhibited by L-pipecolic acid, D-pipecolic acid and isonipecotic acid.1 Publication

Kineticsi

  1. KM=0.172 mM for proline1 Publication

    GO - Molecular functioni

    • glycine transmembrane transporter activity Source: RGD

    GO - Biological processi

    • amino acid export Source: RGD
    • glycine transport Source: GOC
    • positive regulation of glycine import Source: RGD
    • proline transmembrane transport Source: RGD
    • regulation of proline transport Source: RGD
    • regulation of proton transport Source: RGD
    Complete GO annotation...

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Proton-coupled amino acid transporter 2
    Short name:
    Proton/amino acid transporter 2
    Short name:
    rPAT2
    Alternative name(s):
    Solute carrier family 36 member 2
    Tramdorin-1
    Gene namesi
    Name:Slc36a2
    Synonyms:Pat2, Tramd1
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    Proteomesi
    • UP000002494 Componenti: Unplaced

    Organism-specific databases

    RGDi620492. Slc36a2.

    Subcellular locationi

    Topology

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 5656CytoplasmicSequence analysisAdd
    BLAST
    Transmembranei57 – 7721HelicalSequence analysisAdd
    BLAST
    Topological domaini78 – 792ExtracellularSequence analysis
    Transmembranei80 – 10021HelicalSequence analysisAdd
    BLAST
    Topological domaini101 – 14646CytoplasmicSequence analysisAdd
    BLAST
    Transmembranei147 – 16721HelicalSequence analysisAdd
    BLAST
    Topological domaini168 – 19528ExtracellularSequence analysisAdd
    BLAST
    Transmembranei196 – 21621HelicalSequence analysisAdd
    BLAST
    Topological domaini217 – 2204CytoplasmicSequence analysis
    Transmembranei221 – 24121HelicalSequence analysisAdd
    BLAST
    Topological domaini242 – 26221ExtracellularSequence analysisAdd
    BLAST
    Transmembranei263 – 28321HelicalSequence analysisAdd
    BLAST
    Topological domaini284 – 29512CytoplasmicSequence analysisAdd
    BLAST
    Transmembranei296 – 31621HelicalSequence analysisAdd
    BLAST
    Topological domaini317 – 34327ExtracellularSequence analysisAdd
    BLAST
    Transmembranei344 – 36421HelicalSequence analysisAdd
    BLAST
    Topological domaini365 – 37713CytoplasmicSequence analysisAdd
    BLAST
    Transmembranei378 – 39821HelicalSequence analysisAdd
    BLAST
    Topological domaini399 – 4024ExtracellularSequence analysis
    Transmembranei403 – 42321HelicalSequence analysisAdd
    BLAST
    Topological domaini424 – 44421CytoplasmicSequence analysisAdd
    BLAST
    Transmembranei445 – 46521HelicalSequence analysisAdd
    BLAST
    Topological domaini466 – 48116ExtracellularSequence analysisAdd
    BLAST

    GO - Cellular componenti

    Complete GO annotation...

    Keywords - Cellular componenti

    Cell membrane, Cytoplasm, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 481481Proton-coupled amino acid transporter 2PRO_0000324821Add
    BLAST

    Proteomic databases

    PaxDbiQ8K415.
    PRIDEiQ8K415.

    PTM databases

    iPTMnetiQ8K415.
    PhosphoSiteiQ8K415.

    Expressioni

    Tissue specificityi

    Expressed in lung and spleen, and to a lower extent in brain, heart, kidney and skeletal muscle.1 Publication

    Interactioni

    Protein-protein interaction databases

    STRINGi10116.ENSRNOP00000016440.

    Structurei

    3D structure databases

    ProteinModelPortaliQ8K415.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiKOG1304. Eukaryota.
    COG0814. LUCA.
    HOGENOMiHOG000006823.
    HOVERGENiHBG057183.
    InParanoidiQ8K415.
    KOiK14209.
    PhylomeDBiQ8K415.

    Family and domain databases

    InterProiIPR013057. AA_transpt_TM.
    [Graphical view]
    PfamiPF01490. Aa_trans. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q8K415-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MSVTKSAGSP QVAATVKLDL VSFPESAKKV QSQDPNPVNG SSSESSEKTK
    60 70 80 90 100
    GITGFQTLVH LVKGNMGTGI LGLPLAVKNA GILMGPLSLL VMGLIACHCM
    110 120 130 140 150
    HILVRCAQRF CHRLNKPFMD YGDTVMHGLA SSPNTWLQSH AHWGRHAVSF
    160 170 180 190 200
    FLIVTQLGFC CVYIVFLADN LKQVVEAVNS TTISCHKNET VVLTPTIDSR
    210 220 230 240 250
    LYMLAFLPVL GLLVFIRNLR VLTIFSLLAN VSMLVSLVII GQYIIQGIPD
    260 270 280 290 300
    PSQLPLVASW KTYPLFFGTA IFSFESIGVV LPLENKMKDA RRFPTILSLG
    310 320 330 340 350
    MSIITTLYIA IGALGYLRFG DDIKASITLN LPNCWLYQSV KLLYVVGILC
    360 370 380 390 400
    THALQFYVPA EIIIPLAVSQ VSKRWALPVD LSIRLALVCV TCMLAILIPR
    410 420 430 440 450
    LDLVLSLVGS VSSSALALII PPLLEVTTYY GEGMSPLTIT KDALISILGF
    460 470 480
    MGFVVGTYQA LDELIRSGNS LPLSNSTMFI Q
    Length:481
    Mass (Da):52,278
    Last modified:October 1, 2002 - v1
    Checksum:iFBCB4C32BCB0BB12
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AF512430 mRNA. Translation: AAM44855.1.
    RefSeqiNP_647555.1. NM_139339.1.
    UniGeneiRn.145405.

    Genome annotation databases

    GeneIDi246235.
    KEGGirno:246235.
    UCSCiRGD:620492. rat.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AF512430 mRNA. Translation: AAM44855.1.
    RefSeqiNP_647555.1. NM_139339.1.
    UniGeneiRn.145405.

    3D structure databases

    ProteinModelPortaliQ8K415.
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    STRINGi10116.ENSRNOP00000016440.

    PTM databases

    iPTMnetiQ8K415.
    PhosphoSiteiQ8K415.

    Proteomic databases

    PaxDbiQ8K415.
    PRIDEiQ8K415.

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    GeneIDi246235.
    KEGGirno:246235.
    UCSCiRGD:620492. rat.

    Organism-specific databases

    CTDi153201.
    RGDi620492. Slc36a2.

    Phylogenomic databases

    eggNOGiKOG1304. Eukaryota.
    COG0814. LUCA.
    HOGENOMiHOG000006823.
    HOVERGENiHBG057183.
    InParanoidiQ8K415.
    KOiK14209.
    PhylomeDBiQ8K415.

    Miscellaneous databases

    PROiQ8K415.

    Family and domain databases

    InterProiIPR013057. AA_transpt_TM.
    [Graphical view]
    PfamiPF01490. Aa_trans. 1 hit.
    [Graphical view]
    ProtoNetiSearch...

    Publicationsi

    1. "Identification of genes that are downregulated in the absence of the POU domain transcription factor pou3f1 (Oct-6, Tst-1, SCIP) in sciatic nerve."
      Bermingham J.R. Jr., Shumas S., Whisenhunt T., Sirkowski E.E., O'Connell S., Scherer S.S., Rosenfeld M.G.
      J. Neurosci. 22:10217-10231(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: Sprague-Dawley.
    2. "Structure, tissue expression pattern, and function of the amino acid transporter rat PAT2."
      Chen Z., Kennedy D.J., Wake K.A., Zhuang L., Ganapathy V., Thwaites D.T.
      Biochem. Biophys. Res. Commun. 304:747-754(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: TISSUE SPECIFICITY.
    3. "Substrate specificity and functional characterisation of the H+/amino acid transporter rat PAT2 (Slc36a2)."
      Kennedy D.J., Gatfield K.M., Winpenny J.P., Ganapathy V., Thwaites D.T.
      Br. J. Pharmacol. 144:28-41(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES.

    Entry informationi

    Entry nameiS36A2_RAT
    AccessioniPrimary (citable) accession number: Q8K415
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 18, 2008
    Last sequence update: October 1, 2002
    Last modified: June 8, 2016
    This is version 68 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.