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Protein

Disintegrin and metalloproteinase domain-containing protein 32

Gene

Adam32

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

May play a role in sperm development and fertilization This is a non-catalytic metalloprotease-like protein.

GO - Molecular functioni

Protein family/group databases

MEROPSiM12.960.

Names & Taxonomyi

Protein namesi
Recommended name:
Disintegrin and metalloproteinase domain-containing protein 32
Short name:
ADAM 32
Gene namesi
Name:Adam32
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 8

Organism-specific databases

MGIiMGI:2653822. Adam32.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini177 – 689ExtracellularSequence analysisAdd BLAST513
Transmembranei690 – 710HelicalSequence analysisAdd BLAST21
Topological domaini711 – 754CytoplasmicSequence analysisAdd BLAST44

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 22Sequence analysisAdd BLAST22
PropeptideiPRO_000002914023 – 176Sequence analysisAdd BLAST154
ChainiPRO_0000029141177 – 754Disintegrin and metalloproteinase domain-containing protein 32Add BLAST578

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei23PhosphoserineBy similarity1
Glycosylationi126N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi296 ↔ 379By similarity
Disulfide bondi338 ↔ 363By similarity
Disulfide bondi340 ↔ 345By similarity
Glycosylationi362N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi454 ↔ 475By similarity
Glycosylationi469N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi570N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi571N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi632 ↔ 642By similarity
Disulfide bondi636 ↔ 648By similarity
Disulfide bondi650 ↔ 659By similarity

Keywords - PTMi

Cleavage on pair of basic residues, Disulfide bond, Glycoprotein, Phosphoprotein

Proteomic databases

PaxDbiQ8K410.
PRIDEiQ8K410.

PTM databases

iPTMnetiQ8K410.
PhosphoSitePlusiQ8K410.

Expressioni

Tissue specificityi

Highly expressed in the testis and weakly expressed in the epididymis, brain and heart.1 Publication

Developmental stagei

First detected in the testis at day 16 and was reached to the adult level by day 30 after birth.

Gene expression databases

BgeeiENSMUSG00000037437.
CleanExiMM_ADAM32.
ExpressionAtlasiQ8K410. baseline and differential.
GenevisibleiQ8K410. MM.

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000113627.

Structurei

3D structure databases

ProteinModelPortaliQ8K410.
SMRiQ8K410.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini187 – 384Peptidase M12BPROSITE-ProRule annotationAdd BLAST198
Domaini391 – 483DisintegrinPROSITE-ProRule annotationAdd BLAST93
Domaini628 – 660EGF-likePROSITE-ProRule annotationAdd BLAST33

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi484 – 506Cys-richAdd BLAST23

Keywords - Domaini

EGF-like domain, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3607. Eukaryota.
ENOG410XX2M. LUCA.
GeneTreeiENSGT00760000118888.
HOGENOMiHOG000230883.
HOVERGENiHBG103628.
InParanoidiQ8K410.
KOiK16070.
OMAiPLYLEMH.
OrthoDBiEOG091G02F0.
TreeFamiTF314733.

Family and domain databases

CDDicd04269. ZnMc_adamalysin_II_like. 1 hit.
Gene3Di3.40.390.10. 1 hit.
4.10.70.10. 1 hit.
InterProiView protein in InterPro
IPR006586. ADAM_Cys-rich.
IPR001762. Disintegrin_dom.
IPR036436. Disintegrin_dom_sf.
IPR013032. EGF-like_CS.
IPR000742. EGF-like_dom.
IPR024079. MetalloPept_cat_dom_sf.
IPR001590. Peptidase_M12B.
IPR002870. Peptidase_M12B_N.
IPR034027. Reprolysin_adamalysin.
PfamiView protein in Pfam
PF08516. ADAM_CR. 1 hit.
PF00200. Disintegrin. 1 hit.
PF01562. Pep_M12B_propep. 1 hit.
PF01421. Reprolysin. 1 hit.
SMARTiView protein in SMART
SM00608. ACR. 1 hit.
SM00050. DISIN. 1 hit.
SUPFAMiSSF57552. SSF57552. 1 hit.
PROSITEiView protein in PROSITE
PS50215. ADAM_MEPRO. 1 hit.
PS50214. DISINTEGRIN_2. 1 hit.
PS01186. EGF_2. 1 hit.
PS50026. EGF_3. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8K410-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MLGAMLHTLL LLLLAELGAL LASGPESQSS FLEIIFPEKI EDKTHSEEQI
60 70 80 90 100
SYIIPINKKQ YTVHLQKRYF LTNRFMVYMY NQGSTSFHSP NIPAQCYYQG
110 120 130 140 150
HIKGYPNSVA TLSTCSGLRG FLQFENVSYG IEPLQSAFTS QHIVYKLGNK
160 170 180 190 200
EKELIFNKNS RNIEMPTNYG ILINKKPKSP FKNLFPLYLE MSIVVDKALY
210 220 230 240 250
DYLGSDSNIV TNKIIEIISL INSVFAQLKV TIVLSSLELW SDKNKIPTVG
260 270 280 290 300
EADELLHKFL EWKQAYLTLR PHDVAYLFIY NEYPNYMGAT YPGKMCTAHY
310 320 330 340 350
SAGITMYPKD MTLEAFSVIL TQMLGLSLGI SYDEPEKCYC SESICIMNPR
360 370 380 390 400
AMQYGGVKSF SNCSLNDFEH FKSNEGAKCL QNKPQMQRTA AAVCGNGKVE
410 420 430 440 450
GDEICDCGSE AECGPDSCCE PNRCVLKAGR ACDSKSPSST CCKNCQFLPE
460 470 480 490 500
KHQCRPEKHL YCDIPEVCNG SSGNCPPDVT INNGHVCKES GTICYNGDCP
510 520 530 540 550
DLDRVCESIY GAGSVNAPFA CYEEIQGQND RFGNCGKDNR NRYVFCGWRN
560 570 580 590 600
LICGRLICTY PTRMPYNPPN NSTASVIYAF VRDKVCITVD FGSSVKEDPL
610 620 630 640 650
RVANGATCDL DRICLNGVCV ESRFLRDQSK TCSSKCHGNG VCNSHGVCHC
660 670 680 690 700
NAGYSPPNCQ YPTTKRSASL WSGKHDLPME RASKNQEKKW LLSLYIVLII
710 720 730 740 750
LASVFLIGTG WKGLKQCGSK EEESMSSESK SEDSTYTYVS RSTSETSSMT

STSS
Length:754
Mass (Da):83,985
Last modified:May 3, 2011 - v3
Checksum:i27092719C526934E
GO
Isoform 2 (identifier: Q8K410-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     641-754: VCNSHGVCHC...ETSSMTSTSS → KQIRRQHLHV...EHHNFQPINF

Note: No experimental confirmation available.
Show »
Length:677
Mass (Da):75,935
Checksum:i985CD38F26EDCF9F
GO

Sequence cautioni

The sequence AAH60983 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated
The sequence AAN03858 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti361S → I in AAN03858 (PubMed:12568724).Curated1
Sequence conflicti728E → K in AAH60983 (PubMed:15489334).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_012052641 – 754VCNSH…TSTSS → KQIRRQHLHVCQQVSSLKDG VKRHVENEHHNFQPINF in isoform 2. 1 PublicationAdd BLAST114

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK005759 mRNA. Translation: BAC25124.1.
BC060983 mRNA. Translation: AAH60983.1. Different initiation.
AF513715 mRNA. Translation: AAN03858.1. Different initiation.
CCDSiCCDS40300.2. [Q8K410-1]
CCDS80867.1. [Q8K410-2]
RefSeqiNP_001280622.1. NM_001293693.1. [Q8K410-2]
NP_700446.2. NM_153397.2. [Q8K410-1]
UniGeneiMm.269223.

Genome annotation databases

EnsembliENSMUST00000119720; ENSMUSP00000113076; ENSMUSG00000037437. [Q8K410-2]
ENSMUST00000121438; ENSMUSP00000113627; ENSMUSG00000037437. [Q8K410-1]
GeneIDi353188.
KEGGimmu:353188.
UCSCiuc009lfh.2. mouse. [Q8K410-1]
uc009lfi.2. mouse. [Q8K410-2]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiADA32_MOUSE
AccessioniPrimary (citable) accession number: Q8K410
Secondary accession number(s): Q6P901, Q8BJ80
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 7, 2004
Last sequence update: May 3, 2011
Last modified: November 22, 2017
This is version 121 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot