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Protein

DNA polymerase beta

Gene

Polb

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Repair polymerase that plays a key role in base-excision repair. Has 5'-deoxyribose-5-phosphate lyase (dRP lyase) activity that removes the 5' sugar phosphate and also acts as a DNA polymerase that adds one nucleotide to the 3' end of the arising single-nucleotide gap. Conducts 'gap-filling' DNA synthesis in a stepwise distributive fashion rather than in a processive fashion as for other DNA polymerases (By similarity).By similarity

Catalytic activityi

Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1).

Cofactori

Mg2+By similarityNote: Binds 2 magnesium ions per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei72Schiff-base intermediate with DNABy similarity1
Metal bindingi101Sodium; via carbonyl oxygenBy similarity1
Metal bindingi103Sodium; via carbonyl oxygenBy similarity1
Metal bindingi106Sodium; via carbonyl oxygenBy similarity1
Metal bindingi190Magnesium 1By similarity1
Metal bindingi190Magnesium 2By similarity1
Metal bindingi192Magnesium 1By similarity1
Metal bindingi192Magnesium 2By similarity1
Metal bindingi256Magnesium 2By similarity1

GO - Molecular functioni

GO - Biological processi

  • aging Source: Ensembl
  • apoptotic process Source: MGI
  • base-excision repair Source: UniProtKB
  • base-excision repair, gap-filling Source: MGI
  • cellular response to DNA damage stimulus Source: UniProtKB
  • DNA replication Source: UniProtKB-KW
  • homeostasis of number of cells Source: MGI
  • immunoglobulin heavy chain V-D-J recombination Source: MGI
  • inflammatory response Source: MGI
  • intrinsic apoptotic signaling pathway in response to DNA damage Source: MGI
  • lymph node development Source: MGI
  • neuron apoptotic process Source: MGI
  • pyrimidine dimer repair Source: Ensembl
  • response to ethanol Source: Ensembl
  • response to gamma radiation Source: Ensembl
  • response to hyperoxia Source: Ensembl
  • salivary gland morphogenesis Source: MGI
  • somatic diversification of immunoglobulins Source: MGI
  • somatic hypermutation of immunoglobulin genes Source: MGI
  • spleen development Source: MGI

Keywordsi

Molecular functionDNA-binding, DNA-directed DNA polymerase, Lyase, Nucleotidyltransferase, Transferase
Biological processDNA damage, DNA repair, DNA replication, DNA synthesis
LigandMagnesium, Metal-binding, Sodium

Enzyme and pathway databases

ReactomeiR-MMU-110362. POLB-Dependent Long Patch Base Excision Repair.
R-MMU-110373. Resolution of AP sites via the multiple-nucleotide patch replacement pathway.
R-MMU-110381. Resolution of AP sites via the single-nucleotide replacement pathway.
R-MMU-5649702. APEX1-Independent Resolution of AP Sites via the Single Nucleotide Replacement Pathway.
R-MMU-5651801. PCNA-Dependent Long Patch Base Excision Repair.
R-MMU-5689880. Ub-specific processing proteases.
R-MMU-73930. Abasic sugar-phosphate removal via the single-nucleotide replacement pathway.

Names & Taxonomyi

Protein namesi
Recommended name:
DNA polymerase beta (EC:2.7.7.7, EC:4.2.99.-)
Gene namesi
Name:Polb
OrganismiMus musculus (Mouse)Imported
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 8

Organism-specific databases

MGIiMGI:97740. Polb.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL4565.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002187791 – 335DNA polymerase betaAdd BLAST335

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Cross-linki41Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity
Cross-linki61Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity
Modified residuei72N6-acetyllysineCombined sources1
Cross-linki81Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity
Modified residuei83Omega-N-methylarginine; by PRMT6By similarity1
Modified residuei152Omega-N-methylarginine; by PRMT6By similarity1

Post-translational modificationi

Methylation by PRMT6 stimulates the polymerase activity by enhancing DNA binding and processivity.By similarity
Ubiquitinated at Lys-41, Lys-61 and Lys-81: monoubiquitinated by HUWE1/ARF-BP1. Monoubiquitinated protein is then the target of STUB1/CHIP, which catalyzes polyubiquitination from monoubiquitin, leading to degradation by the proteasome. USP47 mediates the deubiquitination of monoubiquitinated protein, preventing polyubiquitination by STUB1/CHIP and its subsequent degradation (By similarity).By similarity

Keywords - PTMi

Acetylation, Isopeptide bond, Methylation, Ubl conjugation

Proteomic databases

EPDiQ8K409.
MaxQBiQ8K409.
PaxDbiQ8K409.
PeptideAtlasiQ8K409.
PRIDEiQ8K409.

PTM databases

iPTMnetiQ8K409.
PhosphoSitePlusiQ8K409.

Expressioni

Gene expression databases

BgeeiENSMUSG00000031536.
CleanExiMM_POLB.
ExpressionAtlasiQ8K409. baseline and differential.
GenevisibleiQ8K409. MM.

Interactioni

Subunit structurei

Monomer. Interacts with APEX1, HUWE1/ARF-BP1, STUB1/CHIP and USP47 (By similarity). Interacts with FAM168A (By similarity).By similarity

GO - Molecular functioni

Protein-protein interaction databases

BioGridi202289. 2 interactors.
IntActiQ8K409. 2 interactors.
MINTiMINT-4093472.
STRINGi10090.ENSMUSP00000033938.

Chemistry databases

BindingDBiQ8K409.

Structurei

3D structure databases

ProteinModelPortaliQ8K409.
SMRiQ8K409.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni183 – 192DNA bindingBy similarity10

Domaini

Residues 239-252 form a flexible loop which appears to affect the polymerase fidelity.By similarity

Sequence similaritiesi

Belongs to the DNA polymerase type-X family.Curated

Phylogenomic databases

eggNOGiKOG2534. Eukaryota.
COG1796. LUCA.
GeneTreeiENSGT00530000063002.
HOGENOMiHOG000007787.
HOVERGENiHBG002359.
InParanoidiQ8K409.
KOiK02330.
OMAiMGVCQLQ.
OrthoDBiEOG091G0HMG.
PhylomeDBiQ8K409.
TreeFamiTF103002.

Family and domain databases

CDDicd00141. NT_POLXc. 1 hit.
Gene3Di1.10.150.110. 1 hit.
3.30.210.10. 1 hit.
InterProiView protein in InterPro
IPR002054. DNA-dir_DNA_pol_X.
IPR019843. DNA_pol-X_BS.
IPR010996. DNA_pol_b-like_N.
IPR028207. DNA_pol_B_palm_palm.
IPR018944. DNA_pol_lambd_fingers_domain.
IPR027421. DNA_pol_lamdba_lyase_dom_sf.
IPR037160. DNA_Pol_thumb_sf.
IPR022312. DNA_pol_X.
IPR002008. DNA_pol_X_beta-like.
IPR003583. Hlx-hairpin-Hlx_DNA-bd_motif.
IPR029398. PolB_thumb.
PfamiView protein in Pfam
PF14792. DNA_pol_B_palm. 1 hit.
PF14791. DNA_pol_B_thumb. 1 hit.
PF10391. DNA_pol_lambd_f. 1 hit.
PF14716. HHH_8. 1 hit.
PRINTSiPR00869. DNAPOLX.
PR00870. DNAPOLXBETA.
SMARTiView protein in SMART
SM00278. HhH1. 2 hits.
SM00483. POLXc. 1 hit.
SUPFAMiSSF47802. SSF47802. 1 hit.
PROSITEiView protein in PROSITE
PS00522. DNA_POLYMERASE_X. 1 hit.

Sequencei

Sequence statusi: Complete.

Q8K409-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSKRKAPQET LNGGITDMLV ELANFEKNVS QAIHKYNAYR KAASVIAKYP
60 70 80 90 100
HKIKSGAEAK KLPGVGTKIA EKIDEFLATG KLRKLEKIRQ DDTSSSINFL
110 120 130 140 150
TRVTGIGPSA ARKFVDEGIK TLEDLRKNED KLNHHQRIGL KYFEDFEKRI
160 170 180 190 200
PREEMLQMQD IVLNEIKKVD SEYIATVCGS FRRGAESSGD MDVLLTHPNF
210 220 230 240 250
TSESSKQPKL LHRVVEQLQK VHFITDTLSK GETKFMGVCQ LPSEKDGKEY
260 270 280 290 300
PHRRIDIRLI PKDQYYCGVL YFTGSDIFNK NMRAHALEKG FTINEYTIRP
310 320 330
LGVTGVAGEP LPVDSEQDIF DYIQWRYREP KDRSE
Length:335
Mass (Da):38,288
Last modified:January 23, 2007 - v3
Checksum:i41E62348DC766A39
GO

Sequence cautioni

The sequence AAH06681 differs from that shown. Reason: Frameshift at several positions.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF513911 mRNA. Translation: AAM49616.1.
AK077127 mRNA. Translation: BAC36630.1.
AK146745 mRNA. Translation: BAE27405.1.
AK151436 mRNA. Translation: BAE30399.1.
BC006681 mRNA. Translation: AAH06681.1. Sequence problems.
BC060998 mRNA. Translation: AAH60998.1.
CCDSiCCDS22181.1.
RefSeqiNP_035260.1. NM_011130.2.
UniGeneiMm.123211.
Mm.473777.

Genome annotation databases

EnsembliENSMUST00000033938; ENSMUSP00000033938; ENSMUSG00000031536.
GeneIDi18970.
KEGGimmu:18970.
UCSCiuc009ldk.2. mouse.

Similar proteinsi

Entry informationi

Entry nameiDPOLB_MOUSE
AccessioniPrimary (citable) accession number: Q8K409
Secondary accession number(s): Q3UAB6, Q922Z7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 7, 2004
Last sequence update: January 23, 2007
Last modified: November 22, 2017
This is version 140 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families