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Q8K400

- STXB5_MOUSE

UniProt

Q8K400 - STXB5_MOUSE

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Protein

Syntaxin-binding protein 5

Gene

Stxbp5

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Plays a regulatory role in calcium-dependent exocytosis and neurotransmitter release (By similarity). Inhibits membrane fusion between transport vesicles and the plasma membrane. May modulate the assembly of trans-SNARE complexes between transport vesicles and the plasma membrane. Competes with STXBP1 for STX1 binding. Inhibits translocation of GLUT4 from intracellular vesicles to the plasma membrane.By similarity1 Publication

GO - Molecular functioni

  1. syntaxin-1 binding Source: BHF-UCL
  2. syntaxin binding Source: MGI

GO - Biological processi

  1. axonogenesis Source: BHF-UCL
  2. exocytosis Source: MGI
  3. positive regulation of exocytosis Source: BHF-UCL
  4. protein transport Source: UniProtKB-KW
  5. regulation of exocytosis Source: MGI
Complete GO annotation...

Keywords - Biological processi

Exocytosis, Protein transport, Transport

Names & Taxonomyi

Protein namesi
Recommended name:
Syntaxin-binding protein 5
Alternative name(s):
Lethal(2) giant larvae protein homolog 3
Tomosyn-1
Gene namesi
Name:Stxbp5
Synonyms:Kiaa4253, Llgl3
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 10

Organism-specific databases

MGIiMGI:1926058. Stxbp5.

Subcellular locationi

Cytoplasm 1 Publication. Cell membrane 1 Publication; Peripheral membrane protein 1 Publication. Membrane 1 Publication; Peripheral membrane protein 1 Publication
Note: Cytoplasmic, and associated with vesicular membranes and the plasma membrane.

GO - Cellular componenti

  1. acetylcholine-gated channel complex Source: MGI
  2. secretory granule Source: BHF-UCL
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 11521152Syntaxin-binding protein 5PRO_0000051245Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei724 – 7241Phosphoserine; by PKABy similarity
Modified residuei760 – 7601Phosphoserine1 Publication
Modified residuei763 – 7631PhosphothreonineBy similarity
Modified residuei783 – 7831Phosphoserine2 Publications
Modified residuei786 – 7861Phosphoserine2 Publications

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ8K400.
PaxDbiQ8K400.
PRIDEiQ8K400.

PTM databases

PhosphoSiteiQ8K400.

Expressioni

Tissue specificityi

Detected in heart, spleen, lung, skeletal muscle, liver and kidney (at protein level). Detected in brain, particularly in the olfactory bulb and in hippocampus. Detected in the tenia tecta and in the piriform layer of the brain cortex.2 Publications

Gene expression databases

BgeeiQ8K400.
CleanExiMM_STXBP5.
ExpressionAtlasiQ8K400. baseline and differential.
GenevestigatoriQ8K400.

Interactioni

Subunit structurei

Part of a complex that contains STX1, STXBP5, SNAP25 and SYT1 (By similarity). Interacts with STX1A and STX4A via its v-SNARE homology domain. Part of a complex that contains STXBP5, STX4A and SNAP23.By similarity1 Publication

Protein-protein interaction databases

IntActiQ8K400. 3 interactions.
MINTiMINT-269206.

Structurei

3D structure databases

ProteinModelPortaliQ8K400.
SMRiQ8K400. Positions 195-267, 505-531, 1088-1144.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati62 – 9534WD 1Add
BLAST
Repeati102 – 14140WD 2Add
BLAST
Repeati146 – 18237WD 3Add
BLAST
Repeati201 – 23535WD 4Add
BLAST
Repeati241 – 27333WD 5Add
BLAST
Repeati295 – 33743WD 6Add
BLAST
Repeati345 – 37935WD 7Add
BLAST
Repeati401 – 47878WD 8Add
BLAST
Repeati506 – 620115WD 9Add
BLAST
Repeati634 – 69663WD 10Add
BLAST
Repeati795 – 85258WD 11Add
BLAST
Repeati861 – 93575WD 12Add
BLAST
Repeati940 – 98445WD 13Add
BLAST
Repeati998 – 102124WD 14Add
BLAST
Domaini1087 – 114761v-SNARE coiled-coil homologyPROSITE-ProRule annotationAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi22 – 287Poly-Gln
Compositional biasi585 – 5917Poly-Ser

Sequence similaritiesi

Belongs to the WD repeat L(2)GL family.Curated
Contains 1 v-SNARE coiled-coil homology domain.PROSITE-ProRule annotation
Contains 14 WD repeats.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil, Repeat, WD repeat

Phylogenomic databases

eggNOGiCOG2319.
GeneTreeiENSGT00390000000018.
HOGENOMiHOG000110929.
HOVERGENiHBG083064.
InParanoidiQ8K400.
KOiK08518.
OMAiRYTHDEA.
OrthoDBiEOG7DVD96.

Family and domain databases

Gene3Di2.130.10.10. 3 hits.
InterProiIPR000664. Lethal2_giant.
IPR013905. Lgl_C_dom.
IPR013577. LLGL2.
IPR001388. Synaptobrevin.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR019775. WD40_repeat_CS.
IPR017986. WD40_repeat_dom.
[Graphical view]
PfamiPF08596. Lgl_C. 1 hit.
PF08366. LLGL. 1 hit.
PF00400. WD40. 2 hits.
[Graphical view]
PRINTSiPR00962. LETHAL2GIANT.
SMARTiSM00320. WD40. 7 hits.
[Graphical view]
SUPFAMiSSF50978. SSF50978. 7 hits.
PROSITEiPS50892. V_SNARE. 1 hit.
PS00678. WD_REPEATS_1. 2 hits.
PS50082. WD_REPEATS_2. 1 hit.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: Q8K400-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MRKFNIRKVL DGLTAGSSSA SQQQQQQQHP PGNREPEIQE TLQSEHFQLC
60 70 80 90 100
KTVRHGFPYQ PSALAFDPVQ KILAVGTQTG ALRLFGRPGV ECYCQHDSGA
110 120 130 140 150
AVIQLQFLIN EGALVSALAD DTLHLWNLRQ KRPAVLHSLK FCRERVTFCH
160 170 180 190 200
LPFQSKWLYV GTERGNIHIV NVESFTLSGY VIMWNKAIEL SSKAHPGPVV
210 220 230 240 250
HISDNPMDEG KLLIGFESGT VVLWDLKSKK ADYRYTYDEA IHSVAWHHEG
260 270 280 290 300
KQFICSHSDG TLTIWNVRSP AKPVQTITPH GKQLKDGKKP EPCKPILKVE
310 320 330 340 350
LKTTRSGEPF IILSGGLSYD TVGRRPCLTV MHGKSTAVLE MDYSIVDFLT
360 370 380 390 400
LCETPYPNDF QEPYAVVVLL EKDLVLIDLA QNGYPIFENP YPLSIHESPV
410 420 430 440 450
TCCEYFADCP VDLIPALYSV GARQKRQGYS KKEWPINGGN WGLGAQSYPE
460 470 480 490 500
IIITGHADGS VKFWDASAIT LQVLYKLKTS KVFEKSRNKD DRQNTDIVDE
510 520 530 540 550
DPYAIQIISW CPESRMLCIA GVSAHVIIYR FSKQEVLTEV IPMLEVRLLY
560 570 580 590 600
EINDVDTPEG EQPPPLSTPV GSSNPQPIPP QSHPSTSSSS SDGLRDNVPC
610 620 630 640 650
LKVKNSPLKQ SPGYQTELVI QLVWVGGEPP QQITSLALNS SYGLVVFGNC
660 670 680 690 700
NGIAMVDYLQ KAVLLNLSTI ELYGSNDPYR REPRSPRKSR QPSGAGLCDI
710 720 730 740 750
TEGTVVPEDR CKSPTSGSSS PHNSDDEQKV NNFIEKVKTQ SRKFSKMVAN
760 770 780 790 800
DLAKMSRKLS LPTDLKPDLD VKDNSFSRSR SSSVTSIDKE SRETISALHF
810 820 830 840 850
CETLTRKADS SPSPCLWVGT TVGTAFVITL NLPPGPEQRL LQPVIVSPSG
860 870 880 890 900
TILRLKGAIL RMAFLDATGC LMSPAYEPWK EHNVAEEKDE KEKLKKRRPV
910 920 930 940 950
SVSPSSSQEI SENQYAVICS EKQAKVMSLP TQSCAYKQNI TETSFVLRGD
960 970 980 990 1000
IVALSNSVCL ACFCANGHIM TFSLPSLRPL LDVYYLPLTN MRIARTFCFA
1010 1020 1030 1040 1050
NNGQALYLVS PTEIQRLTYS QETCENLQEM LGELFTPVET PEAPNRGFFK
1060 1070 1080 1090 1100
GLFGGGAQSL DREELFGESS SGKASRSLAQ HIPGPGGIEG VKGAASGVVG
1110 1120 1130 1140 1150
ELARARLALD ERGQKLSDLE ERTAAMMSSA DSFSKHAHEM MLKYKDKKWY

QF
Length:1,152
Mass (Da):127,651
Last modified:July 27, 2011 - v3
Checksum:iCB62642C7BE5F376
GO
Isoform 2 (identifier: Q8K400-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-754: Missing.

Note: No experimental confirmation available.

Show »
Length:398
Mass (Da):43,782
Checksum:iED0F48D778207727
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti90 – 901V → A in AAM61882. (PubMed:12832401)Curated
Sequence conflicti194 – 1941A → S in AAM61882. (PubMed:12832401)Curated
Sequence conflicti213 – 2131L → P in AAM61882. (PubMed:12832401)Curated
Sequence conflicti225 – 2251D → E in AAM61882. (PubMed:12832401)Curated
Sequence conflicti238 – 2381D → G in AAM61881. (PubMed:12832401)Curated
Sequence conflicti247 – 2471H → Y in AAM61881. (PubMed:12832401)Curated
Sequence conflicti271 – 2711A → T in AAM61881. (PubMed:12832401)Curated
Sequence conflicti301 – 3011L → F in AAM61882. (PubMed:12832401)Curated
Sequence conflicti811 – 8111S → F in BAB31853. (PubMed:19468303)Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 754754Missing in isoform 2. 1 PublicationVSP_016206Add
BLAST

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF516607 mRNA. Translation: AAM61881.1.
AF516608 mRNA. Translation: AAM61882.1.
AK019788 mRNA. Translation: BAB31853.1.
AC122390 Genomic DNA. No translation available.
AK122417 mRNA. Translation: BAC65699.1.
RefSeqiXP_006512989.1. XM_006512926.1. [Q8K400-1]
UniGeneiMm.331751.

Genome annotation databases

EnsembliENSMUST00000038213; ENSMUSP00000044535; ENSMUSG00000019790. [Q8K400-1]
GeneIDi78808.
KEGGimmu:78808.
UCSCiuc007eiy.1. mouse. [Q8K400-2]
uc007eja.1. mouse. [Q8K400-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF516607 mRNA. Translation: AAM61881.1 .
AF516608 mRNA. Translation: AAM61882.1 .
AK019788 mRNA. Translation: BAB31853.1 .
AC122390 Genomic DNA. No translation available.
AK122417 mRNA. Translation: BAC65699.1 .
RefSeqi XP_006512989.1. XM_006512926.1. [Q8K400-1 ]
UniGenei Mm.331751.

3D structure databases

ProteinModelPortali Q8K400.
SMRi Q8K400. Positions 195-267, 505-531, 1088-1144.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi Q8K400. 3 interactions.
MINTi MINT-269206.

PTM databases

PhosphoSitei Q8K400.

Proteomic databases

MaxQBi Q8K400.
PaxDbi Q8K400.
PRIDEi Q8K400.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000038213 ; ENSMUSP00000044535 ; ENSMUSG00000019790 . [Q8K400-1 ]
GeneIDi 78808.
KEGGi mmu:78808.
UCSCi uc007eiy.1. mouse. [Q8K400-2 ]
uc007eja.1. mouse. [Q8K400-1 ]

Organism-specific databases

CTDi 134957.
MGIi MGI:1926058. Stxbp5.
Rougei Search...

Phylogenomic databases

eggNOGi COG2319.
GeneTreei ENSGT00390000000018.
HOGENOMi HOG000110929.
HOVERGENi HBG083064.
InParanoidi Q8K400.
KOi K08518.
OMAi RYTHDEA.
OrthoDBi EOG7DVD96.

Miscellaneous databases

PROi Q8K400.
SOURCEi Search...

Gene expression databases

Bgeei Q8K400.
CleanExi MM_STXBP5.
ExpressionAtlasi Q8K400. baseline and differential.
Genevestigatori Q8K400.

Family and domain databases

Gene3Di 2.130.10.10. 3 hits.
InterProi IPR000664. Lethal2_giant.
IPR013905. Lgl_C_dom.
IPR013577. LLGL2.
IPR001388. Synaptobrevin.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR019775. WD40_repeat_CS.
IPR017986. WD40_repeat_dom.
[Graphical view ]
Pfami PF08596. Lgl_C. 1 hit.
PF08366. LLGL. 1 hit.
PF00400. WD40. 2 hits.
[Graphical view ]
PRINTSi PR00962. LETHAL2GIANT.
SMARTi SM00320. WD40. 7 hits.
[Graphical view ]
SUPFAMi SSF50978. SSF50978. 7 hits.
PROSITEi PS50892. V_SNARE. 1 hit.
PS00678. WD_REPEATS_1. 2 hits.
PS50082. WD_REPEATS_2. 1 hit.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Tomosyn interacts with the t-SNAREs syntaxin4 and SNAP23 and plays a role in insulin-stimulated GLUT4 translocation."
    Widberg C.H., Bryant N.J., Girotti M., Rea S., James D.E.
    J. Biol. Chem. 278:35093-35101(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, INTERACTION WITH STX1A AND STX4A, IDENTIFICATION IN A COMPLEX WITH STX4A AND SNAP23, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
    Strain: Swiss albino.
    Tissue: Adipocyte.
  2. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    Strain: C57BL/6J.
    Tissue: Testis.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  4. "Prediction of the coding sequences of mouse homologues of KIAA gene: II. The complete nucleotide sequences of 400 mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries."
    Okazaki N., Kikuno R., Ohara R., Inamoto S., Aizawa H., Yuasa S., Nakajima D., Nagase T., Ohara O., Koga H.
    DNA Res. 10:35-48(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 892-1152.
    Tissue: Brain.
  5. "Two distinct genes drive expression of seven tomosyn isoforms in the mammalian brain, sharing a conserved structure with a unique variable domain."
    Groffen A.J.A., Jacobsen L., Schut D., Verhage M.
    J. Neurochem. 92:554-568(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY, ALTERNATIVE SPLICING.
  6. "Comprehensive identification of phosphorylation sites in postsynaptic density preparations."
    Trinidad J.C., Specht C.G., Thalhammer A., Schoepfer R., Burlingame A.L.
    Mol. Cell. Proteomics 5:914-922(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brain.
  7. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-783 AND SER-786, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Liver.
  8. "The phagosomal proteome in interferon-gamma-activated macrophages."
    Trost M., English L., Lemieux S., Courcelles M., Desjardins M., Thibault P.
    Immunity 30:143-154(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-760; SER-783 AND SER-786, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiSTXB5_MOUSE
AccessioniPrimary (citable) accession number: Q8K400
Secondary accession number(s): E9QLY8
, Q80TM5, Q8K401, Q9D2F3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 22, 2005
Last sequence update: July 27, 2011
Last modified: October 29, 2014
This is version 108 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3