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Q8K400

- STXB5_MOUSE

UniProt

Q8K400 - STXB5_MOUSE

Protein

Syntaxin-binding protein 5

Gene

Stxbp5

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 107 (01 Oct 2014)
      Sequence version 3 (27 Jul 2011)
      Previous versions | rss
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    Functioni

    Plays a regulatory role in calcium-dependent exocytosis and neurotransmitter release By similarity. Inhibits membrane fusion between transport vesicles and the plasma membrane. May modulate the assembly of trans-SNARE complexes between transport vesicles and the plasma membrane. Competes with STXBP1 for STX1 binding. Inhibits translocation of GLUT4 from intracellular vesicles to the plasma membrane.By similarity1 Publication

    GO - Molecular functioni

    1. protein binding Source: MGI
    2. syntaxin-1 binding Source: BHF-UCL
    3. syntaxin binding Source: MGI

    GO - Biological processi

    1. axonogenesis Source: BHF-UCL
    2. exocytosis Source: MGI
    3. positive regulation of exocytosis Source: BHF-UCL
    4. protein transport Source: UniProtKB-KW
    5. regulation of exocytosis Source: MGI

    Keywords - Biological processi

    Exocytosis, Protein transport, Transport

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Syntaxin-binding protein 5
    Alternative name(s):
    Lethal(2) giant larvae protein homolog 3
    Tomosyn-1
    Gene namesi
    Name:Stxbp5
    Synonyms:Kiaa4253, Llgl3
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 10

    Organism-specific databases

    MGIiMGI:1926058. Stxbp5.

    Subcellular locationi

    Cytoplasm 1 Publication. Cell membrane 1 Publication; Peripheral membrane protein 1 Publication. Membrane 1 Publication; Peripheral membrane protein 1 Publication
    Note: Cytoplasmic, and associated with vesicular membranes and the plasma membrane.

    GO - Cellular componenti

    1. acetylcholine-gated channel complex Source: MGI
    2. secretory granule Source: BHF-UCL

    Keywords - Cellular componenti

    Cell membrane, Cytoplasm, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 11521152Syntaxin-binding protein 5PRO_0000051245Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei724 – 7241Phosphoserine; by PKABy similarity
    Modified residuei760 – 7601Phosphoserine1 Publication
    Modified residuei763 – 7631PhosphothreonineBy similarity
    Modified residuei783 – 7831Phosphoserine2 Publications
    Modified residuei786 – 7861Phosphoserine2 Publications

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiQ8K400.
    PaxDbiQ8K400.
    PRIDEiQ8K400.

    PTM databases

    PhosphoSiteiQ8K400.

    Expressioni

    Tissue specificityi

    Detected in heart, spleen, lung, skeletal muscle, liver and kidney (at protein level). Detected in brain, particularly in the olfactory bulb and in hippocampus. Detected in the tenia tecta and in the piriform layer of the brain cortex.2 Publications

    Gene expression databases

    ArrayExpressiQ8K400.
    BgeeiQ8K400.
    CleanExiMM_STXBP5.
    GenevestigatoriQ8K400.

    Interactioni

    Subunit structurei

    Part of a complex that contains STX1, STXBP5, SNAP25 and SYT1 By similarity. Interacts with STX1A and STX4A via its v-SNARE homology domain. Part of a complex that contains STXBP5, STX4A and SNAP23.By similarity1 Publication

    Protein-protein interaction databases

    IntActiQ8K400. 3 interactions.
    MINTiMINT-269206.

    Structurei

    3D structure databases

    ProteinModelPortaliQ8K400.
    SMRiQ8K400. Positions 440-472, 1088-1144.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Repeati62 – 9534WD 1Add
    BLAST
    Repeati102 – 14140WD 2Add
    BLAST
    Repeati146 – 18237WD 3Add
    BLAST
    Repeati201 – 23535WD 4Add
    BLAST
    Repeati241 – 27333WD 5Add
    BLAST
    Repeati295 – 33743WD 6Add
    BLAST
    Repeati345 – 37935WD 7Add
    BLAST
    Repeati401 – 47878WD 8Add
    BLAST
    Repeati506 – 620115WD 9Add
    BLAST
    Repeati634 – 69663WD 10Add
    BLAST
    Repeati795 – 85258WD 11Add
    BLAST
    Repeati861 – 93575WD 12Add
    BLAST
    Repeati940 – 98445WD 13Add
    BLAST
    Repeati998 – 102124WD 14Add
    BLAST
    Domaini1087 – 114761v-SNARE coiled-coil homologyPROSITE-ProRule annotationAdd
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi22 – 287Poly-Gln
    Compositional biasi585 – 5917Poly-Ser

    Sequence similaritiesi

    Belongs to the WD repeat L(2)GL family.Curated
    Contains 1 v-SNARE coiled-coil homology domain.PROSITE-ProRule annotation
    Contains 14 WD repeats.PROSITE-ProRule annotation

    Keywords - Domaini

    Coiled coil, Repeat, WD repeat

    Phylogenomic databases

    eggNOGiCOG2319.
    GeneTreeiENSGT00390000000018.
    HOGENOMiHOG000110929.
    HOVERGENiHBG083064.
    KOiK08518.
    OMAiRYTHDEA.
    OrthoDBiEOG7DVD96.

    Family and domain databases

    Gene3Di2.130.10.10. 3 hits.
    InterProiIPR000664. Lethal2_giant.
    IPR013905. Lgl_C_dom.
    IPR013577. LLGL2.
    IPR001388. Synaptobrevin.
    IPR015943. WD40/YVTN_repeat-like_dom.
    IPR001680. WD40_repeat.
    IPR019775. WD40_repeat_CS.
    IPR017986. WD40_repeat_dom.
    [Graphical view]
    PfamiPF08596. Lgl_C. 1 hit.
    PF08366. LLGL. 1 hit.
    PF00400. WD40. 2 hits.
    [Graphical view]
    PRINTSiPR00962. LETHAL2GIANT.
    SMARTiSM00320. WD40. 7 hits.
    [Graphical view]
    SUPFAMiSSF50978. SSF50978. 7 hits.
    PROSITEiPS50892. V_SNARE. 1 hit.
    PS00678. WD_REPEATS_1. 2 hits.
    PS50082. WD_REPEATS_2. 1 hit.
    PS50294. WD_REPEATS_REGION. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q8K400-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MRKFNIRKVL DGLTAGSSSA SQQQQQQQHP PGNREPEIQE TLQSEHFQLC     50
    KTVRHGFPYQ PSALAFDPVQ KILAVGTQTG ALRLFGRPGV ECYCQHDSGA 100
    AVIQLQFLIN EGALVSALAD DTLHLWNLRQ KRPAVLHSLK FCRERVTFCH 150
    LPFQSKWLYV GTERGNIHIV NVESFTLSGY VIMWNKAIEL SSKAHPGPVV 200
    HISDNPMDEG KLLIGFESGT VVLWDLKSKK ADYRYTYDEA IHSVAWHHEG 250
    KQFICSHSDG TLTIWNVRSP AKPVQTITPH GKQLKDGKKP EPCKPILKVE 300
    LKTTRSGEPF IILSGGLSYD TVGRRPCLTV MHGKSTAVLE MDYSIVDFLT 350
    LCETPYPNDF QEPYAVVVLL EKDLVLIDLA QNGYPIFENP YPLSIHESPV 400
    TCCEYFADCP VDLIPALYSV GARQKRQGYS KKEWPINGGN WGLGAQSYPE 450
    IIITGHADGS VKFWDASAIT LQVLYKLKTS KVFEKSRNKD DRQNTDIVDE 500
    DPYAIQIISW CPESRMLCIA GVSAHVIIYR FSKQEVLTEV IPMLEVRLLY 550
    EINDVDTPEG EQPPPLSTPV GSSNPQPIPP QSHPSTSSSS SDGLRDNVPC 600
    LKVKNSPLKQ SPGYQTELVI QLVWVGGEPP QQITSLALNS SYGLVVFGNC 650
    NGIAMVDYLQ KAVLLNLSTI ELYGSNDPYR REPRSPRKSR QPSGAGLCDI 700
    TEGTVVPEDR CKSPTSGSSS PHNSDDEQKV NNFIEKVKTQ SRKFSKMVAN 750
    DLAKMSRKLS LPTDLKPDLD VKDNSFSRSR SSSVTSIDKE SRETISALHF 800
    CETLTRKADS SPSPCLWVGT TVGTAFVITL NLPPGPEQRL LQPVIVSPSG 850
    TILRLKGAIL RMAFLDATGC LMSPAYEPWK EHNVAEEKDE KEKLKKRRPV 900
    SVSPSSSQEI SENQYAVICS EKQAKVMSLP TQSCAYKQNI TETSFVLRGD 950
    IVALSNSVCL ACFCANGHIM TFSLPSLRPL LDVYYLPLTN MRIARTFCFA 1000
    NNGQALYLVS PTEIQRLTYS QETCENLQEM LGELFTPVET PEAPNRGFFK 1050
    GLFGGGAQSL DREELFGESS SGKASRSLAQ HIPGPGGIEG VKGAASGVVG 1100
    ELARARLALD ERGQKLSDLE ERTAAMMSSA DSFSKHAHEM MLKYKDKKWY 1150
    QF 1152
    Length:1,152
    Mass (Da):127,651
    Last modified:July 27, 2011 - v3
    Checksum:iCB62642C7BE5F376
    GO
    Isoform 2 (identifier: Q8K400-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         1-754: Missing.

    Note: No experimental confirmation available.

    Show »
    Length:398
    Mass (Da):43,782
    Checksum:iED0F48D778207727
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti90 – 901V → A in AAM61882. (PubMed:12832401)Curated
    Sequence conflicti194 – 1941A → S in AAM61882. (PubMed:12832401)Curated
    Sequence conflicti213 – 2131L → P in AAM61882. (PubMed:12832401)Curated
    Sequence conflicti225 – 2251D → E in AAM61882. (PubMed:12832401)Curated
    Sequence conflicti238 – 2381D → G in AAM61881. (PubMed:12832401)Curated
    Sequence conflicti247 – 2471H → Y in AAM61881. (PubMed:12832401)Curated
    Sequence conflicti271 – 2711A → T in AAM61881. (PubMed:12832401)Curated
    Sequence conflicti301 – 3011L → F in AAM61882. (PubMed:12832401)Curated
    Sequence conflicti811 – 8111S → F in BAB31853. (PubMed:19468303)Curated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei1 – 754754Missing in isoform 2. 1 PublicationVSP_016206Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF516607 mRNA. Translation: AAM61881.1.
    AF516608 mRNA. Translation: AAM61882.1.
    AK019788 mRNA. Translation: BAB31853.1.
    AC122390 Genomic DNA. No translation available.
    AK122417 mRNA. Translation: BAC65699.1.
    RefSeqiXP_006512989.1. XM_006512926.1. [Q8K400-1]
    UniGeneiMm.331751.

    Genome annotation databases

    EnsembliENSMUST00000038213; ENSMUSP00000044535; ENSMUSG00000019790. [Q8K400-1]
    GeneIDi78808.
    KEGGimmu:78808.
    UCSCiuc007eiy.1. mouse. [Q8K400-2]
    uc007eja.1. mouse. [Q8K400-1]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF516607 mRNA. Translation: AAM61881.1 .
    AF516608 mRNA. Translation: AAM61882.1 .
    AK019788 mRNA. Translation: BAB31853.1 .
    AC122390 Genomic DNA. No translation available.
    AK122417 mRNA. Translation: BAC65699.1 .
    RefSeqi XP_006512989.1. XM_006512926.1. [Q8K400-1 ]
    UniGenei Mm.331751.

    3D structure databases

    ProteinModelPortali Q8K400.
    SMRi Q8K400. Positions 440-472, 1088-1144.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi Q8K400. 3 interactions.
    MINTi MINT-269206.

    PTM databases

    PhosphoSitei Q8K400.

    Proteomic databases

    MaxQBi Q8K400.
    PaxDbi Q8K400.
    PRIDEi Q8K400.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000038213 ; ENSMUSP00000044535 ; ENSMUSG00000019790 . [Q8K400-1 ]
    GeneIDi 78808.
    KEGGi mmu:78808.
    UCSCi uc007eiy.1. mouse. [Q8K400-2 ]
    uc007eja.1. mouse. [Q8K400-1 ]

    Organism-specific databases

    CTDi 134957.
    MGIi MGI:1926058. Stxbp5.
    Rougei Search...

    Phylogenomic databases

    eggNOGi COG2319.
    GeneTreei ENSGT00390000000018.
    HOGENOMi HOG000110929.
    HOVERGENi HBG083064.
    KOi K08518.
    OMAi RYTHDEA.
    OrthoDBi EOG7DVD96.

    Miscellaneous databases

    PROi Q8K400.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q8K400.
    Bgeei Q8K400.
    CleanExi MM_STXBP5.
    Genevestigatori Q8K400.

    Family and domain databases

    Gene3Di 2.130.10.10. 3 hits.
    InterProi IPR000664. Lethal2_giant.
    IPR013905. Lgl_C_dom.
    IPR013577. LLGL2.
    IPR001388. Synaptobrevin.
    IPR015943. WD40/YVTN_repeat-like_dom.
    IPR001680. WD40_repeat.
    IPR019775. WD40_repeat_CS.
    IPR017986. WD40_repeat_dom.
    [Graphical view ]
    Pfami PF08596. Lgl_C. 1 hit.
    PF08366. LLGL. 1 hit.
    PF00400. WD40. 2 hits.
    [Graphical view ]
    PRINTSi PR00962. LETHAL2GIANT.
    SMARTi SM00320. WD40. 7 hits.
    [Graphical view ]
    SUPFAMi SSF50978. SSF50978. 7 hits.
    PROSITEi PS50892. V_SNARE. 1 hit.
    PS00678. WD_REPEATS_1. 2 hits.
    PS50082. WD_REPEATS_2. 1 hit.
    PS50294. WD_REPEATS_REGION. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Tomosyn interacts with the t-SNAREs syntaxin4 and SNAP23 and plays a role in insulin-stimulated GLUT4 translocation."
      Widberg C.H., Bryant N.J., Girotti M., Rea S., James D.E.
      J. Biol. Chem. 278:35093-35101(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, INTERACTION WITH STX1A AND STX4A, IDENTIFICATION IN A COMPLEX WITH STX4A AND SNAP23, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
      Strain: Swiss albino.
      Tissue: Adipocyte.
    2. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
      Strain: C57BL/6J.
      Tissue: Testis.
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: C57BL/6J.
    4. "Prediction of the coding sequences of mouse homologues of KIAA gene: II. The complete nucleotide sequences of 400 mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries."
      Okazaki N., Kikuno R., Ohara R., Inamoto S., Aizawa H., Yuasa S., Nakajima D., Nagase T., Ohara O., Koga H.
      DNA Res. 10:35-48(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 892-1152.
      Tissue: Brain.
    5. "Two distinct genes drive expression of seven tomosyn isoforms in the mammalian brain, sharing a conserved structure with a unique variable domain."
      Groffen A.J.A., Jacobsen L., Schut D., Verhage M.
      J. Neurochem. 92:554-568(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: TISSUE SPECIFICITY, ALTERNATIVE SPLICING.
    6. "Comprehensive identification of phosphorylation sites in postsynaptic density preparations."
      Trinidad J.C., Specht C.G., Thalhammer A., Schoepfer R., Burlingame A.L.
      Mol. Cell. Proteomics 5:914-922(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Brain.
    7. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-783 AND SER-786, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Liver.
    8. "The phagosomal proteome in interferon-gamma-activated macrophages."
      Trost M., English L., Lemieux S., Courcelles M., Desjardins M., Thibault P.
      Immunity 30:143-154(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-760; SER-783 AND SER-786, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiSTXB5_MOUSE
    AccessioniPrimary (citable) accession number: Q8K400
    Secondary accession number(s): E9QLY8
    , Q80TM5, Q8K401, Q9D2F3
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 22, 2005
    Last sequence update: July 27, 2011
    Last modified: October 1, 2014
    This is version 107 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3