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Protein

Syntaxin-binding protein 5

Gene

Stxbp5

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Plays a regulatory role in calcium-dependent exocytosis and neurotransmitter release (By similarity). Inhibits membrane fusion between transport vesicles and the plasma membrane. May modulate the assembly of trans-SNARE complexes between transport vesicles and the plasma membrane. Competes with STXBP1 for STX1 binding. Inhibits translocation of GLUT4 from intracellular vesicles to the plasma membrane.By similarity1 Publication

GO - Molecular functioni

  • GTPase activator activity Source: GO_Central
  • Rab GTPase binding Source: GO_Central
  • syntaxin-1 binding Source: BHF-UCL
  • syntaxin binding Source: MGI

GO - Biological processi

  • axonogenesis Source: BHF-UCL
  • exocytosis Source: MGI
  • positive regulation of exocytosis Source: BHF-UCL
  • protein transport Source: UniProtKB-KW
  • regulation of blood coagulation Source: MGI
  • regulation of exocytosis Source: MGI
  • regulation of gene expression Source: MGI
  • regulation of protein secretion Source: GO_Central
Complete GO annotation...

Keywords - Biological processi

Exocytosis, Protein transport, Transport

Names & Taxonomyi

Protein namesi
Recommended name:
Syntaxin-binding protein 5
Alternative name(s):
Lethal(2) giant larvae protein homolog 3
Tomosyn-1
Gene namesi
Name:Stxbp5
Synonyms:Kiaa4253, Llgl3
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 10

Organism-specific databases

MGIiMGI:1926058. Stxbp5.

Subcellular locationi

  • Cytoplasm 1 Publication
  • Cell membrane 1 Publication; Peripheral membrane protein 1 Publication
  • Membrane 1 Publication; Peripheral membrane protein 1 Publication

  • Note: Cytoplasmic, and associated with vesicular membranes and the plasma membrane.

GO - Cellular componenti

  • acetylcholine-gated channel complex Source: MGI
  • cytoplasm Source: MGI
  • secretory granule Source: BHF-UCL
  • SNARE complex Source: GO_Central
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000512451 – 1152Syntaxin-binding protein 5Add BLAST1152

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei693PhosphoserineBy similarity1
Modified residuei724PhosphoserineCombined sources1
Modified residuei760PhosphoserineCombined sources1
Modified residuei763PhosphothreonineBy similarity1
Modified residuei783PhosphoserineCombined sources1
Modified residuei785PhosphothreonineCombined sources1
Modified residuei786PhosphoserineCombined sources1
Modified residuei901PhosphoserineCombined sources1
Modified residuei903PhosphoserineCombined sources1
Modified residuei1040PhosphothreonineBy similarity1
Modified residuei1059PhosphoserineCombined sources1
Modified residuei1132PhosphoserineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ8K400.
PaxDbiQ8K400.
PeptideAtlasiQ8K400.
PRIDEiQ8K400.

PTM databases

iPTMnetiQ8K400.
PhosphoSitePlusiQ8K400.
SwissPalmiQ8K400.

Expressioni

Tissue specificityi

Detected in heart, spleen, lung, skeletal muscle, liver and kidney (at protein level). Detected in brain, particularly in the olfactory bulb and in hippocampus. Detected in the tenia tecta and in the piriform layer of the brain cortex.2 Publications

Gene expression databases

BgeeiENSMUSG00000019790.
CleanExiMM_STXBP5.
ExpressionAtlasiQ8K400. baseline and differential.
GenevisibleiQ8K400. MM.

Interactioni

Subunit structurei

Part of a complex that contains STX1, STXBP5, SNAP25 and SYT1 (By similarity). Interacts with STX1A and STX4A via its v-SNARE homology domain. Part of a complex that contains STXBP5, STX4A and SNAP23.By similarity1 Publication

GO - Molecular functioni

  • Rab GTPase binding Source: GO_Central
  • syntaxin-1 binding Source: BHF-UCL
  • syntaxin binding Source: MGI

Protein-protein interaction databases

IntActiQ8K400. 3 interactors.
MINTiMINT-269206.
STRINGi10090.ENSMUSP00000123253.

Structurei

3D structure databases

ProteinModelPortaliQ8K400.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati62 – 95WD 1Add BLAST34
Repeati102 – 141WD 2Add BLAST40
Repeati146 – 182WD 3Add BLAST37
Repeati201 – 235WD 4Add BLAST35
Repeati241 – 273WD 5Add BLAST33
Repeati295 – 337WD 6Add BLAST43
Repeati345 – 379WD 7Add BLAST35
Repeati401 – 478WD 8Add BLAST78
Repeati506 – 620WD 9Add BLAST115
Repeati634 – 696WD 10Add BLAST63
Repeati795 – 852WD 11Add BLAST58
Repeati861 – 935WD 12Add BLAST75
Repeati940 – 984WD 13Add BLAST45
Repeati998 – 1021WD 14Add BLAST24
Domaini1087 – 1147v-SNARE coiled-coil homologyPROSITE-ProRule annotationAdd BLAST61

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi22 – 28Poly-Gln7
Compositional biasi585 – 591Poly-Ser7

Sequence similaritiesi

Belongs to the WD repeat L(2)GL family.Curated
Contains 1 v-SNARE coiled-coil homology domain.PROSITE-ProRule annotation
Contains 14 WD repeats.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil, Repeat, WD repeat

Phylogenomic databases

eggNOGiENOG410INFG. Eukaryota.
ENOG410XQV3. LUCA.
GeneTreeiENSGT00390000000018.
HOGENOMiHOG000110929.
HOVERGENiHBG083064.
InParanoidiQ8K400.
OMAiRYTHDEA.
OrthoDBiEOG091G0LY3.

Family and domain databases

Gene3Di2.130.10.10. 3 hits.
InterProiIPR000664. Lethal2_giant.
IPR013905. Lgl_C_dom.
IPR013577. LLGL2.
IPR001388. Synaptobrevin.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR019775. WD40_repeat_CS.
IPR017986. WD40_repeat_dom.
[Graphical view]
PfamiPF08596. Lgl_C. 1 hit.
PF08366. LLGL. 1 hit.
PF00400. WD40. 1 hit.
[Graphical view]
PRINTSiPR00962. LETHAL2GIANT.
SMARTiSM00320. WD40. 7 hits.
[Graphical view]
SUPFAMiSSF50978. SSF50978. 7 hits.
PROSITEiPS50892. V_SNARE. 1 hit.
PS00678. WD_REPEATS_1. 2 hits.
PS50082. WD_REPEATS_2. 1 hit.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8K400-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MRKFNIRKVL DGLTAGSSSA SQQQQQQQHP PGNREPEIQE TLQSEHFQLC
60 70 80 90 100
KTVRHGFPYQ PSALAFDPVQ KILAVGTQTG ALRLFGRPGV ECYCQHDSGA
110 120 130 140 150
AVIQLQFLIN EGALVSALAD DTLHLWNLRQ KRPAVLHSLK FCRERVTFCH
160 170 180 190 200
LPFQSKWLYV GTERGNIHIV NVESFTLSGY VIMWNKAIEL SSKAHPGPVV
210 220 230 240 250
HISDNPMDEG KLLIGFESGT VVLWDLKSKK ADYRYTYDEA IHSVAWHHEG
260 270 280 290 300
KQFICSHSDG TLTIWNVRSP AKPVQTITPH GKQLKDGKKP EPCKPILKVE
310 320 330 340 350
LKTTRSGEPF IILSGGLSYD TVGRRPCLTV MHGKSTAVLE MDYSIVDFLT
360 370 380 390 400
LCETPYPNDF QEPYAVVVLL EKDLVLIDLA QNGYPIFENP YPLSIHESPV
410 420 430 440 450
TCCEYFADCP VDLIPALYSV GARQKRQGYS KKEWPINGGN WGLGAQSYPE
460 470 480 490 500
IIITGHADGS VKFWDASAIT LQVLYKLKTS KVFEKSRNKD DRQNTDIVDE
510 520 530 540 550
DPYAIQIISW CPESRMLCIA GVSAHVIIYR FSKQEVLTEV IPMLEVRLLY
560 570 580 590 600
EINDVDTPEG EQPPPLSTPV GSSNPQPIPP QSHPSTSSSS SDGLRDNVPC
610 620 630 640 650
LKVKNSPLKQ SPGYQTELVI QLVWVGGEPP QQITSLALNS SYGLVVFGNC
660 670 680 690 700
NGIAMVDYLQ KAVLLNLSTI ELYGSNDPYR REPRSPRKSR QPSGAGLCDI
710 720 730 740 750
TEGTVVPEDR CKSPTSGSSS PHNSDDEQKV NNFIEKVKTQ SRKFSKMVAN
760 770 780 790 800
DLAKMSRKLS LPTDLKPDLD VKDNSFSRSR SSSVTSIDKE SRETISALHF
810 820 830 840 850
CETLTRKADS SPSPCLWVGT TVGTAFVITL NLPPGPEQRL LQPVIVSPSG
860 870 880 890 900
TILRLKGAIL RMAFLDATGC LMSPAYEPWK EHNVAEEKDE KEKLKKRRPV
910 920 930 940 950
SVSPSSSQEI SENQYAVICS EKQAKVMSLP TQSCAYKQNI TETSFVLRGD
960 970 980 990 1000
IVALSNSVCL ACFCANGHIM TFSLPSLRPL LDVYYLPLTN MRIARTFCFA
1010 1020 1030 1040 1050
NNGQALYLVS PTEIQRLTYS QETCENLQEM LGELFTPVET PEAPNRGFFK
1060 1070 1080 1090 1100
GLFGGGAQSL DREELFGESS SGKASRSLAQ HIPGPGGIEG VKGAASGVVG
1110 1120 1130 1140 1150
ELARARLALD ERGQKLSDLE ERTAAMMSSA DSFSKHAHEM MLKYKDKKWY

QF
Length:1,152
Mass (Da):127,651
Last modified:July 27, 2011 - v3
Checksum:iCB62642C7BE5F376
GO
Isoform 2 (identifier: Q8K400-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-754: Missing.

Note: No experimental confirmation available.
Show »
Length:398
Mass (Da):43,782
Checksum:iED0F48D778207727
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti90V → A in AAM61882 (PubMed:12832401).Curated1
Sequence conflicti194A → S in AAM61882 (PubMed:12832401).Curated1
Sequence conflicti213L → P in AAM61882 (PubMed:12832401).Curated1
Sequence conflicti225D → E in AAM61882 (PubMed:12832401).Curated1
Sequence conflicti238D → G in AAM61881 (PubMed:12832401).Curated1
Sequence conflicti247H → Y in AAM61881 (PubMed:12832401).Curated1
Sequence conflicti271A → T in AAM61881 (PubMed:12832401).Curated1
Sequence conflicti301L → F in AAM61882 (PubMed:12832401).Curated1
Sequence conflicti811S → F in BAB31853 (PubMed:19468303).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0162061 – 754Missing in isoform 2. 1 PublicationAdd BLAST754

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF516607 mRNA. Translation: AAM61881.1.
AF516608 mRNA. Translation: AAM61882.1.
AK019788 mRNA. Translation: BAB31853.1.
AC122390 Genomic DNA. No translation available.
AK122417 mRNA. Translation: BAC65699.1.
RefSeqiXP_006512989.1. XM_006512926.3. [Q8K400-1]
UniGeneiMm.331751.

Genome annotation databases

EnsembliENSMUST00000038213; ENSMUSP00000044535; ENSMUSG00000019790. [Q8K400-1]
GeneIDi78808.
UCSCiuc007eiy.1. mouse. [Q8K400-2]
uc007eja.1. mouse. [Q8K400-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF516607 mRNA. Translation: AAM61881.1.
AF516608 mRNA. Translation: AAM61882.1.
AK019788 mRNA. Translation: BAB31853.1.
AC122390 Genomic DNA. No translation available.
AK122417 mRNA. Translation: BAC65699.1.
RefSeqiXP_006512989.1. XM_006512926.3. [Q8K400-1]
UniGeneiMm.331751.

3D structure databases

ProteinModelPortaliQ8K400.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ8K400. 3 interactors.
MINTiMINT-269206.
STRINGi10090.ENSMUSP00000123253.

PTM databases

iPTMnetiQ8K400.
PhosphoSitePlusiQ8K400.
SwissPalmiQ8K400.

Proteomic databases

EPDiQ8K400.
PaxDbiQ8K400.
PeptideAtlasiQ8K400.
PRIDEiQ8K400.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000038213; ENSMUSP00000044535; ENSMUSG00000019790. [Q8K400-1]
GeneIDi78808.
UCSCiuc007eiy.1. mouse. [Q8K400-2]
uc007eja.1. mouse. [Q8K400-1]

Organism-specific databases

CTDi134957.
MGIiMGI:1926058. Stxbp5.
RougeiSearch...

Phylogenomic databases

eggNOGiENOG410INFG. Eukaryota.
ENOG410XQV3. LUCA.
GeneTreeiENSGT00390000000018.
HOGENOMiHOG000110929.
HOVERGENiHBG083064.
InParanoidiQ8K400.
OMAiRYTHDEA.
OrthoDBiEOG091G0LY3.

Miscellaneous databases

PROiQ8K400.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000019790.
CleanExiMM_STXBP5.
ExpressionAtlasiQ8K400. baseline and differential.
GenevisibleiQ8K400. MM.

Family and domain databases

Gene3Di2.130.10.10. 3 hits.
InterProiIPR000664. Lethal2_giant.
IPR013905. Lgl_C_dom.
IPR013577. LLGL2.
IPR001388. Synaptobrevin.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR019775. WD40_repeat_CS.
IPR017986. WD40_repeat_dom.
[Graphical view]
PfamiPF08596. Lgl_C. 1 hit.
PF08366. LLGL. 1 hit.
PF00400. WD40. 1 hit.
[Graphical view]
PRINTSiPR00962. LETHAL2GIANT.
SMARTiSM00320. WD40. 7 hits.
[Graphical view]
SUPFAMiSSF50978. SSF50978. 7 hits.
PROSITEiPS50892. V_SNARE. 1 hit.
PS00678. WD_REPEATS_1. 2 hits.
PS50082. WD_REPEATS_2. 1 hit.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSTXB5_MOUSE
AccessioniPrimary (citable) accession number: Q8K400
Secondary accession number(s): E9QLY8
, Q80TM5, Q8K401, Q9D2F3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 22, 2005
Last sequence update: July 27, 2011
Last modified: November 2, 2016
This is version 128 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.