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Protein

Ankyrin repeat and SAM domain-containing protein 4B

Gene

Anks4b

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

As part of the intermicrovillar adhesion complex/IMAC plays a role in epithelial brush border differentiation, controlling microvilli organization and length. Plays a role in assembly of the complex (By similarity). May play a role in cellular response to endoplasmic reticulum stress (PubMed:22589549).By similarity1 Publication

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Differentiation

Names & Taxonomyi

Protein namesi
Recommended name:
Ankyrin repeat and SAM domain-containing protein 4BCurated
Alternative name(s):
Harmonin-interacting ankyrin repeat-containing protein1 Publication
Short name:
Harp1 Publication
Gene namesi
Name:Anks4bImported
Synonyms:Harp1 Publication
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 7

Organism-specific databases

MGIiMGI:1919324. Anks4b.

Subcellular locationi

GO - Cellular componenti

  • brush border Source: UniProtKB
  • endoplasmic reticulum membrane Source: MGI
  • microvillus Source: UniProtKB
  • plasma membrane Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cell projection

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000669971 – 423Ankyrin repeat and SAM domain-containing protein 4BAdd BLAST423

Proteomic databases

MaxQBiQ8K3X6.
PaxDbiQ8K3X6.
PeptideAtlasiQ8K3X6.
PRIDEiQ8K3X6.

PTM databases

iPTMnetiQ8K3X6.
PhosphoSitePlusiQ8K3X6.

Expressioni

Tissue specificityi

Cochlea, kidney, lung, liver, pancreas, salivary gland and small intestine (at protein level). Expressed in kidney, small intestine, pancreas, liver and colon. Not detected in heart, spleen and brain.2 Publications

Gene expression databases

BgeeiENSMUSG00000030909.
GenevisibleiQ8K3X6. MM.

Interactioni

Subunit structurei

Part of the IMAC/intermicrovillar adhesion complex/intermicrovillar tip-link complex composed of ANKS4B, MYO7B, USH1C, CDHR2 and CDHR5 (By similarity). Interacts with USH1C; the interaction is direct and is required for ANKS4B localization to the tip of microvilli (PubMed:15461667, PubMed:26812017). Interacts with MYO7B; the interaction is direct (PubMed:26812017). May interact with HSPA5 (PubMed:22589549).By similarity3 Publications

Protein-protein interaction databases

BioGridi215131. 1 interactor.
IntActiQ8K3X6. 1 interactor.
STRINGi10090.ENSMUSP00000033201.

Structurei

Secondary structure

1423
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi265 – 267Combined sources3
Turni268 – 270Combined sources3
Helixi353 – 360Combined sources8
Helixi364 – 366Combined sources3
Helixi367 – 372Combined sources6
Helixi377 – 381Combined sources5
Helixi385 – 390Combined sources6
Helixi395 – 413Combined sources19
Beta strandi421 – 423Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5F3XX-ray2.65B/D345-423[»]
5F3YX-ray3.41B252-352[»]
ProteinModelPortaliQ8K3X6.
SMRiQ8K3X6.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati31 – 60ANK 1Add BLAST30
Repeati64 – 93ANK 2Add BLAST30
Repeati97 – 126ANK 3Add BLAST30
Domaini357 – 409SAMAdd BLAST53

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 251Mediates localization to microvilliBy similarityAdd BLAST251
Regioni253 – 352Mediates interaction with MYO7B1 PublicationAdd BLAST100

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili130 – 169Sequence analysisAdd BLAST40
Coiled coili301 – 335Sequence analysisAdd BLAST35

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi421 – 423PDZ-binding; mediates interaction with USH1C1 Publication3

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi312 – 346Glu-richAdd BLAST35

Sequence similaritiesi

Contains 3 ANK repeats.PROSITE-ProRule annotation

Keywords - Domaini

ANK repeat, Coiled coil, Repeat

Phylogenomic databases

eggNOGiKOG0504. Eukaryota.
COG0666. LUCA.
GeneTreeiENSGT00390000017548.
HOGENOMiHOG000007847.
HOVERGENiHBG051882.
InParanoidiQ8K3X6.
OMAiNGHAHCV.
OrthoDBiEOG091G0DIP.
PhylomeDBiQ8K3X6.
TreeFamiTF324946.

Family and domain databases

Gene3Di1.10.150.50. 1 hit.
1.25.40.20. 1 hit.
InterProiIPR002110. Ankyrin_rpt.
IPR020683. Ankyrin_rpt-contain_dom.
IPR001660. SAM.
IPR013761. SAM/pointed.
[Graphical view]
PfamiPF12796. Ank_2. 1 hit.
PF00536. SAM_1. 1 hit.
[Graphical view]
SMARTiSM00248. ANK. 3 hits.
SM00454. SAM. 1 hit.
[Graphical view]
SUPFAMiSSF47769. SSF47769. 1 hit.
SSF48403. SSF48403. 1 hit.
PROSITEiPS50297. ANK_REP_REGION. 1 hit.
PS50088. ANK_REPEAT. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8K3X6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSTRYHQAAS DSYLELLKEA TKRDLNLSDE DGMTPTLLAA YHGNLEALEI
60 70 80 90 100
ICSRGGDPDK CDIWGNTPLH YAASNGHTHC ISFLVNFGAN IFALDNDLKS
110 120 130 140 150
PLDAAASREQ KECVALLDKA ATVQNTMNPK RVTRLKEQAL KNARKQMKEC
160 170 180 190 200
ERLQERHQNK MARTYSKEDS GTISSSHSTL SRSFLSTTSA GRFGSLSKGI
210 220 230 240 250
KDTFKIKSKK NKDTTEQLEK DGRSGQRPVM EVFREEEEDS FPKDFKEKLH
260 270 280 290 300
FSVEEDDDVQ HESILNRPGL GSIVFSRNRV LDFEDISDSK RELGFKMPSE
310 320 330 340 350
LFQRQGAAGT VEEEEEEEEE EEEEKREANG TAGDLPWDEE EVEWEEDAVD
360 370 380 390 400
ATPLEVFLQS QHLEEFLPIF MREQIDLEAL LLCSDEDLQN IHMQLGPRKK
410 420
VLSAIDKRKQ VLQQPGQLVD TSL
Length:423
Mass (Da):47,975
Last modified:October 1, 2002 - v1
Checksum:i65886645CB15AB48
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti324E → G in BAB25545 (PubMed:16141072).Curated1
Sequence conflicti332A → E in BAB25545 (PubMed:16141072).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF524030 mRNA. Translation: AAM81375.1.
AK008229 mRNA. Translation: BAB25545.1.
BC115784 mRNA. Translation: AAI15785.1.
BC118013 mRNA. Translation: AAI18014.1.
CCDSiCCDS21793.1.
RefSeqiNP_082361.2. NM_028085.2.
UniGeneiMm.23165.

Genome annotation databases

EnsembliENSMUST00000033201; ENSMUSP00000033201; ENSMUSG00000030909.
GeneIDi72074.
KEGGimmu:72074.
UCSCiuc009jmj.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF524030 mRNA. Translation: AAM81375.1.
AK008229 mRNA. Translation: BAB25545.1.
BC115784 mRNA. Translation: AAI15785.1.
BC118013 mRNA. Translation: AAI18014.1.
CCDSiCCDS21793.1.
RefSeqiNP_082361.2. NM_028085.2.
UniGeneiMm.23165.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5F3XX-ray2.65B/D345-423[»]
5F3YX-ray3.41B252-352[»]
ProteinModelPortaliQ8K3X6.
SMRiQ8K3X6.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi215131. 1 interactor.
IntActiQ8K3X6. 1 interactor.
STRINGi10090.ENSMUSP00000033201.

PTM databases

iPTMnetiQ8K3X6.
PhosphoSitePlusiQ8K3X6.

Proteomic databases

MaxQBiQ8K3X6.
PaxDbiQ8K3X6.
PeptideAtlasiQ8K3X6.
PRIDEiQ8K3X6.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000033201; ENSMUSP00000033201; ENSMUSG00000030909.
GeneIDi72074.
KEGGimmu:72074.
UCSCiuc009jmj.2. mouse.

Organism-specific databases

CTDi257629.
MGIiMGI:1919324. Anks4b.

Phylogenomic databases

eggNOGiKOG0504. Eukaryota.
COG0666. LUCA.
GeneTreeiENSGT00390000017548.
HOGENOMiHOG000007847.
HOVERGENiHBG051882.
InParanoidiQ8K3X6.
OMAiNGHAHCV.
OrthoDBiEOG091G0DIP.
PhylomeDBiQ8K3X6.
TreeFamiTF324946.

Miscellaneous databases

PROiQ8K3X6.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000030909.
GenevisibleiQ8K3X6. MM.

Family and domain databases

Gene3Di1.10.150.50. 1 hit.
1.25.40.20. 1 hit.
InterProiIPR002110. Ankyrin_rpt.
IPR020683. Ankyrin_rpt-contain_dom.
IPR001660. SAM.
IPR013761. SAM/pointed.
[Graphical view]
PfamiPF12796. Ank_2. 1 hit.
PF00536. SAM_1. 1 hit.
[Graphical view]
SMARTiSM00248. ANK. 3 hits.
SM00454. SAM. 1 hit.
[Graphical view]
SUPFAMiSSF47769. SSF47769. 1 hit.
SSF48403. SSF48403. 1 hit.
PROSITEiPS50297. ANK_REP_REGION. 1 hit.
PS50088. ANK_REPEAT. 2 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiANS4B_MOUSE
AccessioniPrimary (citable) accession number: Q8K3X6
Secondary accession number(s): Q148R3, Q9D8A5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 9, 2004
Last sequence update: October 1, 2002
Last modified: November 2, 2016
This is version 110 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.