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Protein

Ankyrin repeat and SAM domain-containing protein 4B

Gene

Anks4b

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

GO - Biological processi

  1. response to endoplasmic reticulum stress Source: MGI
Complete GO annotation...

Names & Taxonomyi

Protein namesi
Recommended name:
Ankyrin repeat and SAM domain-containing protein 4B
Alternative name(s):
Harmonin-interacting ankyrin repeat-containing protein
Short name:
Harp
Gene namesi
Name:Anks4b
Synonyms:Harp
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 7

Organism-specific databases

MGIiMGI:1919324. Anks4b.

Subcellular locationi

GO - Cellular componenti

  1. endoplasmic reticulum membrane Source: MGI
  2. plasma membrane Source: MGI
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 423423Ankyrin repeat and SAM domain-containing protein 4BPRO_0000066997Add
BLAST

Proteomic databases

MaxQBiQ8K3X6.
PaxDbiQ8K3X6.
PRIDEiQ8K3X6.

PTM databases

PhosphoSiteiQ8K3X6.

Expressioni

Tissue specificityi

Cochlea, kidney, lung, liver, pancreas, salivary gland and small intestine (at protein level). Expressed in kidney, small intestine, pancreas, liver and colon. Not detected in heart, spleen and brain.

Gene expression databases

BgeeiQ8K3X6.
GenevestigatoriQ8K3X6.

Interactioni

Subunit structurei

Interacts through its C-terminus with USH1C PDZ 1 domain.1 Publication

Protein-protein interaction databases

BioGridi215131. 1 interaction.
IntActiQ8K3X6. 1 interaction.
STRINGi10090.ENSMUSP00000033201.

Structurei

3D structure databases

ProteinModelPortaliQ8K3X6.
SMRiQ8K3X6. Positions 2-154, 350-423.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati31 – 6030ANK 1Add
BLAST
Repeati64 – 9330ANK 2Add
BLAST
Repeati97 – 12630ANK 3Add
BLAST
Domaini357 – 40953SAMAdd
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili130 – 16940Sequence AnalysisAdd
BLAST
Coiled coili301 – 33535Sequence AnalysisAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi421 – 4233PDZ-binding

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi312 – 34635Glu-richAdd
BLAST

Sequence similaritiesi

Contains 3 ANK repeats.PROSITE-ProRule annotation

Keywords - Domaini

ANK repeat, Coiled coil, Repeat

Phylogenomic databases

eggNOGiCOG0666.
GeneTreeiENSGT00390000017548.
HOGENOMiHOG000007847.
HOVERGENiHBG051882.
InParanoidiQ8K3X6.
OMAiNGHAHCV.
OrthoDBiEOG70GMFR.
PhylomeDBiQ8K3X6.
TreeFamiTF324946.

Family and domain databases

Gene3Di1.10.150.50. 1 hit.
1.25.40.20. 1 hit.
InterProiIPR002110. Ankyrin_rpt.
IPR020683. Ankyrin_rpt-contain_dom.
IPR001660. SAM.
IPR013761. SAM/pointed.
IPR021129. SAM_type1.
[Graphical view]
PfamiPF12796. Ank_2. 1 hit.
PF00536. SAM_1. 1 hit.
[Graphical view]
SMARTiSM00248. ANK. 3 hits.
SM00454. SAM. 1 hit.
[Graphical view]
SUPFAMiSSF47769. SSF47769. 1 hit.
SSF48403. SSF48403. 1 hit.
PROSITEiPS50297. ANK_REP_REGION. 1 hit.
PS50088. ANK_REPEAT. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8K3X6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSTRYHQAAS DSYLELLKEA TKRDLNLSDE DGMTPTLLAA YHGNLEALEI
60 70 80 90 100
ICSRGGDPDK CDIWGNTPLH YAASNGHTHC ISFLVNFGAN IFALDNDLKS
110 120 130 140 150
PLDAAASREQ KECVALLDKA ATVQNTMNPK RVTRLKEQAL KNARKQMKEC
160 170 180 190 200
ERLQERHQNK MARTYSKEDS GTISSSHSTL SRSFLSTTSA GRFGSLSKGI
210 220 230 240 250
KDTFKIKSKK NKDTTEQLEK DGRSGQRPVM EVFREEEEDS FPKDFKEKLH
260 270 280 290 300
FSVEEDDDVQ HESILNRPGL GSIVFSRNRV LDFEDISDSK RELGFKMPSE
310 320 330 340 350
LFQRQGAAGT VEEEEEEEEE EEEEKREANG TAGDLPWDEE EVEWEEDAVD
360 370 380 390 400
ATPLEVFLQS QHLEEFLPIF MREQIDLEAL LLCSDEDLQN IHMQLGPRKK
410 420
VLSAIDKRKQ VLQQPGQLVD TSL
Length:423
Mass (Da):47,975
Last modified:September 30, 2002 - v1
Checksum:i65886645CB15AB48
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti324 – 3241E → G in BAB25545 (PubMed:16141072).Curated
Sequence conflicti332 – 3321A → E in BAB25545 (PubMed:16141072).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF524030 mRNA. Translation: AAM81375.1.
AK008229 mRNA. Translation: BAB25545.1.
BC115784 mRNA. Translation: AAI15785.1.
BC118013 mRNA. Translation: AAI18014.1.
CCDSiCCDS21793.1.
RefSeqiNP_082361.2. NM_028085.2.
UniGeneiMm.23165.

Genome annotation databases

EnsembliENSMUST00000033201; ENSMUSP00000033201; ENSMUSG00000030909.
GeneIDi72074.
KEGGimmu:72074.
UCSCiuc009jmj.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF524030 mRNA. Translation: AAM81375.1.
AK008229 mRNA. Translation: BAB25545.1.
BC115784 mRNA. Translation: AAI15785.1.
BC118013 mRNA. Translation: AAI18014.1.
CCDSiCCDS21793.1.
RefSeqiNP_082361.2. NM_028085.2.
UniGeneiMm.23165.

3D structure databases

ProteinModelPortaliQ8K3X6.
SMRiQ8K3X6. Positions 2-154, 350-423.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi215131. 1 interaction.
IntActiQ8K3X6. 1 interaction.
STRINGi10090.ENSMUSP00000033201.

PTM databases

PhosphoSiteiQ8K3X6.

Proteomic databases

MaxQBiQ8K3X6.
PaxDbiQ8K3X6.
PRIDEiQ8K3X6.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000033201; ENSMUSP00000033201; ENSMUSG00000030909.
GeneIDi72074.
KEGGimmu:72074.
UCSCiuc009jmj.2. mouse.

Organism-specific databases

CTDi257629.
MGIiMGI:1919324. Anks4b.

Phylogenomic databases

eggNOGiCOG0666.
GeneTreeiENSGT00390000017548.
HOGENOMiHOG000007847.
HOVERGENiHBG051882.
InParanoidiQ8K3X6.
OMAiNGHAHCV.
OrthoDBiEOG70GMFR.
PhylomeDBiQ8K3X6.
TreeFamiTF324946.

Miscellaneous databases

NextBioi335364.
PROiQ8K3X6.
SOURCEiSearch...

Gene expression databases

BgeeiQ8K3X6.
GenevestigatoriQ8K3X6.

Family and domain databases

Gene3Di1.10.150.50. 1 hit.
1.25.40.20. 1 hit.
InterProiIPR002110. Ankyrin_rpt.
IPR020683. Ankyrin_rpt-contain_dom.
IPR001660. SAM.
IPR013761. SAM/pointed.
IPR021129. SAM_type1.
[Graphical view]
PfamiPF12796. Ank_2. 1 hit.
PF00536. SAM_1. 1 hit.
[Graphical view]
SMARTiSM00248. ANK. 3 hits.
SM00454. SAM. 1 hit.
[Graphical view]
SUPFAMiSSF47769. SSF47769. 1 hit.
SSF48403. SSF48403. 1 hit.
PROSITEiPS50297. ANK_REP_REGION. 1 hit.
PS50088. ANK_REPEAT. 2 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Harp (harmonin-interacting, ankyrin repeat-containing protein), a novel protein that interacts with harmonin in epithelial tissues."
    Johnston A.M., Naselli G., Niwa H., Brodnicki T., Harrison L.C., Gonez L.J.
    Genes Cells 9:967-982(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], INTERACTION WITH USH1C.
    Strain: BALB/c.
    Tissue: Kidney.
  2. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Small intestine.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  4. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Liver.

Entry informationi

Entry nameiANS4B_MOUSE
AccessioniPrimary (citable) accession number: Q8K3X6
Secondary accession number(s): Q148R3, Q9D8A5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 8, 2004
Last sequence update: September 30, 2002
Last modified: January 6, 2015
This is version 99 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.