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Protein

Ankyrin repeat and SAM domain-containing protein 4B

Gene

Anks4b

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

As part of the intermicrovillar adhesion complex/IMAC plays a role in epithelial brush border differentiation, controlling microvilli organization and length. Plays a role in assembly of the complex (By similarity). May play a role in cellular response to endoplasmic reticulum stress (PubMed:22589549).By similarity1 Publication

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Differentiation

Names & Taxonomyi

Protein namesi
Recommended name:
Ankyrin repeat and SAM domain-containing protein 4BCurated
Alternative name(s):
Harmonin-interacting ankyrin repeat-containing protein1 Publication
Short name:
Harp1 Publication
Gene namesi
Name:Anks4bImported
Synonyms:Harp1 Publication
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 7

Organism-specific databases

MGIiMGI:1919324. Anks4b.

Subcellular locationi

GO - Cellular componenti

  • brush border Source: UniProtKB
  • endoplasmic reticulum membrane Source: MGI
  • microvillus Source: UniProtKB
  • plasma membrane Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cell projection

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 423423Ankyrin repeat and SAM domain-containing protein 4BPRO_0000066997Add
BLAST

Proteomic databases

MaxQBiQ8K3X6.
PaxDbiQ8K3X6.
PRIDEiQ8K3X6.

PTM databases

iPTMnetiQ8K3X6.
PhosphoSiteiQ8K3X6.

Expressioni

Tissue specificityi

Cochlea, kidney, lung, liver, pancreas, salivary gland and small intestine (at protein level). Expressed in kidney, small intestine, pancreas, liver and colon. Not detected in heart, spleen and brain.2 Publications

Gene expression databases

BgeeiQ8K3X6.
GenevisibleiQ8K3X6. MM.

Interactioni

Subunit structurei

Part of the IMAC/intermicrovillar adhesion complex/intermicrovillar tip-link complex composed of ANKS4B, MYO7B, USH1C, CDHR2 and CDHR5 (By similarity). Interacts with USH1C; the interaction is direct and is required for ANKS4B localization to the tip of microvilli (PubMed:15461667, PubMed:26812017). Interacts with MYO7B; the interaction is direct (PubMed:26812017). May interact with HSPA5 (PubMed:22589549).By similarity3 Publications

Protein-protein interaction databases

BioGridi215131. 1 interaction.
IntActiQ8K3X6. 1 interaction.
STRINGi10090.ENSMUSP00000033201.

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
5F3XX-ray2.65B/D345-423[»]
5F3YX-ray3.41B252-352[»]
ProteinModelPortaliQ8K3X6.
SMRiQ8K3X6. Positions 2-154, 350-423.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati31 – 6030ANK 1Add
BLAST
Repeati64 – 9330ANK 2Add
BLAST
Repeati97 – 12630ANK 3Add
BLAST
Domaini357 – 40953SAMAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 251251Mediates localization to microvilliBy similarityAdd
BLAST
Regioni253 – 352100Mediates interaction with MYO7B1 PublicationAdd
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili130 – 16940Sequence analysisAdd
BLAST
Coiled coili301 – 33535Sequence analysisAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi421 – 4233PDZ-binding; mediates interaction with USH1C1 Publication

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi312 – 34635Glu-richAdd
BLAST

Sequence similaritiesi

Contains 3 ANK repeats.PROSITE-ProRule annotation

Keywords - Domaini

ANK repeat, Coiled coil, Repeat

Phylogenomic databases

eggNOGiKOG0504. Eukaryota.
COG0666. LUCA.
GeneTreeiENSGT00390000017548.
HOGENOMiHOG000007847.
HOVERGENiHBG051882.
InParanoidiQ8K3X6.
OMAiQMKECER.
OrthoDBiEOG70GMFR.
PhylomeDBiQ8K3X6.
TreeFamiTF324946.

Family and domain databases

Gene3Di1.10.150.50. 1 hit.
1.25.40.20. 1 hit.
InterProiIPR002110. Ankyrin_rpt.
IPR020683. Ankyrin_rpt-contain_dom.
IPR001660. SAM.
IPR013761. SAM/pointed.
[Graphical view]
PfamiPF12796. Ank_2. 1 hit.
PF00536. SAM_1. 1 hit.
[Graphical view]
SMARTiSM00248. ANK. 3 hits.
SM00454. SAM. 1 hit.
[Graphical view]
SUPFAMiSSF47769. SSF47769. 1 hit.
SSF48403. SSF48403. 1 hit.
PROSITEiPS50297. ANK_REP_REGION. 1 hit.
PS50088. ANK_REPEAT. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8K3X6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSTRYHQAAS DSYLELLKEA TKRDLNLSDE DGMTPTLLAA YHGNLEALEI
60 70 80 90 100
ICSRGGDPDK CDIWGNTPLH YAASNGHTHC ISFLVNFGAN IFALDNDLKS
110 120 130 140 150
PLDAAASREQ KECVALLDKA ATVQNTMNPK RVTRLKEQAL KNARKQMKEC
160 170 180 190 200
ERLQERHQNK MARTYSKEDS GTISSSHSTL SRSFLSTTSA GRFGSLSKGI
210 220 230 240 250
KDTFKIKSKK NKDTTEQLEK DGRSGQRPVM EVFREEEEDS FPKDFKEKLH
260 270 280 290 300
FSVEEDDDVQ HESILNRPGL GSIVFSRNRV LDFEDISDSK RELGFKMPSE
310 320 330 340 350
LFQRQGAAGT VEEEEEEEEE EEEEKREANG TAGDLPWDEE EVEWEEDAVD
360 370 380 390 400
ATPLEVFLQS QHLEEFLPIF MREQIDLEAL LLCSDEDLQN IHMQLGPRKK
410 420
VLSAIDKRKQ VLQQPGQLVD TSL
Length:423
Mass (Da):47,975
Last modified:October 1, 2002 - v1
Checksum:i65886645CB15AB48
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti324 – 3241E → G in BAB25545 (PubMed:16141072).Curated
Sequence conflicti332 – 3321A → E in BAB25545 (PubMed:16141072).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF524030 mRNA. Translation: AAM81375.1.
AK008229 mRNA. Translation: BAB25545.1.
BC115784 mRNA. Translation: AAI15785.1.
BC118013 mRNA. Translation: AAI18014.1.
CCDSiCCDS21793.1.
RefSeqiNP_082361.2. NM_028085.2.
UniGeneiMm.23165.

Genome annotation databases

EnsembliENSMUST00000033201; ENSMUSP00000033201; ENSMUSG00000030909.
GeneIDi72074.
KEGGimmu:72074.
UCSCiuc009jmj.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF524030 mRNA. Translation: AAM81375.1.
AK008229 mRNA. Translation: BAB25545.1.
BC115784 mRNA. Translation: AAI15785.1.
BC118013 mRNA. Translation: AAI18014.1.
CCDSiCCDS21793.1.
RefSeqiNP_082361.2. NM_028085.2.
UniGeneiMm.23165.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
5F3XX-ray2.65B/D345-423[»]
5F3YX-ray3.41B252-352[»]
ProteinModelPortaliQ8K3X6.
SMRiQ8K3X6. Positions 2-154, 350-423.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi215131. 1 interaction.
IntActiQ8K3X6. 1 interaction.
STRINGi10090.ENSMUSP00000033201.

PTM databases

iPTMnetiQ8K3X6.
PhosphoSiteiQ8K3X6.

Proteomic databases

MaxQBiQ8K3X6.
PaxDbiQ8K3X6.
PRIDEiQ8K3X6.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000033201; ENSMUSP00000033201; ENSMUSG00000030909.
GeneIDi72074.
KEGGimmu:72074.
UCSCiuc009jmj.2. mouse.

Organism-specific databases

CTDi257629.
MGIiMGI:1919324. Anks4b.

Phylogenomic databases

eggNOGiKOG0504. Eukaryota.
COG0666. LUCA.
GeneTreeiENSGT00390000017548.
HOGENOMiHOG000007847.
HOVERGENiHBG051882.
InParanoidiQ8K3X6.
OMAiQMKECER.
OrthoDBiEOG70GMFR.
PhylomeDBiQ8K3X6.
TreeFamiTF324946.

Miscellaneous databases

NextBioi335364.
PROiQ8K3X6.
SOURCEiSearch...

Gene expression databases

BgeeiQ8K3X6.
GenevisibleiQ8K3X6. MM.

Family and domain databases

Gene3Di1.10.150.50. 1 hit.
1.25.40.20. 1 hit.
InterProiIPR002110. Ankyrin_rpt.
IPR020683. Ankyrin_rpt-contain_dom.
IPR001660. SAM.
IPR013761. SAM/pointed.
[Graphical view]
PfamiPF12796. Ank_2. 1 hit.
PF00536. SAM_1. 1 hit.
[Graphical view]
SMARTiSM00248. ANK. 3 hits.
SM00454. SAM. 1 hit.
[Graphical view]
SUPFAMiSSF47769. SSF47769. 1 hit.
SSF48403. SSF48403. 1 hit.
PROSITEiPS50297. ANK_REP_REGION. 1 hit.
PS50088. ANK_REPEAT. 2 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Harp (harmonin-interacting, ankyrin repeat-containing protein), a novel protein that interacts with harmonin in epithelial tissues."
    Johnston A.M., Naselli G., Niwa H., Brodnicki T., Harrison L.C., Gonez L.J.
    Genes Cells 9:967-982(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], INTERACTION WITH USH1C, MOTIF, TISSUE SPECIFICITY.
    Strain: BALB/cJ.
    Tissue: Kidney.
  2. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Small intestine.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  4. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Liver.
  5. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Kidney.
  6. "Anks4b, a novel target of HNF4alpha protein, interacts with GRP78 protein and regulates endoplasmic reticulum stress-induced apoptosis in pancreatic beta-cells."
    Sato Y., Hatta M., Karim M.F., Sawa T., Wei F.Y., Sato S., Magnuson M.A., Gonzalez F.J., Tomizawa K., Akaike T., Yoshizawa T., Yamagata K.
    J. Biol. Chem. 287:23236-23245(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH HSPA5, SUBCELLULAR LOCATION.
  7. "ANKS4B is essential for intermicrovillar adhesion complex formation."
    Crawley S.W., Weck M.L., Grega-Larson N.E., Shifrin D.A. Jr., Tyska M.J.
    Dev. Cell 36:190-200(2016) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
  8. "Mechanistic basis of organization of the Harmonin/USH1C-mediated brush border microvilli tip-link complex."
    Li J., He Y., Lu Q., Zhang M.
    Dev. Cell 36:179-189(2016) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.65 ANGSTROMS) OF 345-423 IN COMPLEX WITH USH1C, X-RAY CRYSTALLOGRAPHY (3.4 ANGSTROMS) OF 253-352 IN COMPLEX WITH MYO7B, REGION.

Entry informationi

Entry nameiANS4B_MOUSE
AccessioniPrimary (citable) accession number: Q8K3X6
Secondary accession number(s): Q148R3, Q9D8A5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 9, 2004
Last sequence update: October 1, 2002
Last modified: May 11, 2016
This is version 105 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.