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Q8K3X6 (ANS4B_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 95. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Ankyrin repeat and SAM domain-containing protein 4B
Alternative name(s):
Harmonin-interacting ankyrin repeat-containing protein
Short name=Harp
Gene names
Name:Anks4b
Synonyms:Harp
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length423 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Subunit structure

Interacts through its C-terminus with USH1C PDZ 1 domain. Ref.1

Tissue specificity

Cochlea, kidney, lung, liver, pancreas, salivary gland and small intestine (at protein level). Expressed in kidney, small intestine, pancreas, liver and colon. Not detected in heart, spleen and brain.

Sequence similarities

Contains 3 ANK repeats.

Contains 1 SAM (sterile alpha motif) domain.

Ontologies

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 423423Ankyrin repeat and SAM domain-containing protein 4B
PRO_0000066997

Regions

Repeat31 – 6030ANK 1
Repeat64 – 9330ANK 2
Repeat97 – 12630ANK 3
Domain357 – 40953SAM
Coiled coil130 – 16940 Potential
Coiled coil301 – 33535 Potential
Motif421 – 4233PDZ-binding
Compositional bias312 – 34635Glu-rich

Experimental info

Sequence conflict3241E → G in BAB25545. Ref.2
Sequence conflict3321A → E in BAB25545. Ref.2

Sequences

Sequence LengthMass (Da)Tools
Q8K3X6 [UniParc].

Last modified October 1, 2002. Version 1.
Checksum: 65886645CB15AB48

FASTA42347,975
        10         20         30         40         50         60 
MSTRYHQAAS DSYLELLKEA TKRDLNLSDE DGMTPTLLAA YHGNLEALEI ICSRGGDPDK 

        70         80         90        100        110        120 
CDIWGNTPLH YAASNGHTHC ISFLVNFGAN IFALDNDLKS PLDAAASREQ KECVALLDKA 

       130        140        150        160        170        180 
ATVQNTMNPK RVTRLKEQAL KNARKQMKEC ERLQERHQNK MARTYSKEDS GTISSSHSTL 

       190        200        210        220        230        240 
SRSFLSTTSA GRFGSLSKGI KDTFKIKSKK NKDTTEQLEK DGRSGQRPVM EVFREEEEDS 

       250        260        270        280        290        300 
FPKDFKEKLH FSVEEDDDVQ HESILNRPGL GSIVFSRNRV LDFEDISDSK RELGFKMPSE 

       310        320        330        340        350        360 
LFQRQGAAGT VEEEEEEEEE EEEEKREANG TAGDLPWDEE EVEWEEDAVD ATPLEVFLQS 

       370        380        390        400        410        420 
QHLEEFLPIF MREQIDLEAL LLCSDEDLQN IHMQLGPRKK VLSAIDKRKQ VLQQPGQLVD 


TSL 

« Hide

References

« Hide 'large scale' references
[1]"Harp (harmonin-interacting, ankyrin repeat-containing protein), a novel protein that interacts with harmonin in epithelial tissues."
Johnston A.M., Naselli G., Niwa H., Brodnicki T., Harrison L.C., Gonez L.J.
Genes Cells 9:967-982(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], INTERACTION WITH USH1C.
Strain: BALB/c.
Tissue: Kidney.
[2]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: C57BL/6J.
Tissue: Small intestine.
[3]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[4]"Large-scale phosphorylation analysis of mouse liver."
Villen J., Beausoleil S.A., Gerber S.A., Gygi S.P.
Proc. Natl. Acad. Sci. U.S.A. 104:1488-1493(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Liver.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF524030 mRNA. Translation: AAM81375.1.
AK008229 mRNA. Translation: BAB25545.1.
BC115784 mRNA. Translation: AAI15785.1.
BC118013 mRNA. Translation: AAI18014.1.
CCDSCCDS21793.1.
RefSeqNP_082361.2. NM_028085.2.
UniGeneMm.23165.

3D structure databases

ProteinModelPortalQ8K3X6.
SMRQ8K3X6. Positions 14-159, 350-423.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid215131. 1 interaction.
IntActQ8K3X6. 1 interaction.
STRING10090.ENSMUSP00000033201.

PTM databases

PhosphoSiteQ8K3X6.

Proteomic databases

MaxQBQ8K3X6.
PaxDbQ8K3X6.
PRIDEQ8K3X6.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000033201; ENSMUSP00000033201; ENSMUSG00000030909.
GeneID72074.
KEGGmmu:72074.
UCSCuc009jmj.2. mouse.

Organism-specific databases

CTD257629.
MGIMGI:1919324. Anks4b.

Phylogenomic databases

eggNOGCOG0666.
GeneTreeENSGT00390000017548.
HOGENOMHOG000007847.
HOVERGENHBG051882.
InParanoidQ148R3.
OMANGHAHCV.
OrthoDBEOG70GMFR.
PhylomeDBQ8K3X6.
TreeFamTF324946.

Gene expression databases

BgeeQ8K3X6.
GenevestigatorQ8K3X6.

Family and domain databases

Gene3D1.10.150.50. 1 hit.
1.25.40.20. 1 hit.
InterProIPR002110. Ankyrin_rpt.
IPR020683. Ankyrin_rpt-contain_dom.
IPR001660. SAM.
IPR013761. SAM/pointed.
IPR021129. SAM_type1.
[Graphical view]
PfamPF12796. Ank_2. 1 hit.
PF00536. SAM_1. 1 hit.
[Graphical view]
SMARTSM00248. ANK. 3 hits.
SM00454. SAM. 1 hit.
[Graphical view]
SUPFAMSSF47769. SSF47769. 1 hit.
SSF48403. SSF48403. 1 hit.
PROSITEPS50297. ANK_REP_REGION. 1 hit.
PS50088. ANK_REPEAT. 2 hits.
[Graphical view]
ProtoNetSearch...

Other

NextBio335364.
PROQ8K3X6.
SOURCESearch...

Entry information

Entry nameANS4B_MOUSE
AccessionPrimary (citable) accession number: Q8K3X6
Secondary accession number(s): Q148R3, Q9D8A5
Entry history
Integrated into UniProtKB/Swiss-Prot: November 9, 2004
Last sequence update: October 1, 2002
Last modified: July 9, 2014
This is version 95 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot