Q8K3X3 (GMDS_CRIGR) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
GDP-mannose 4,6 dehydratase
EC=4.2.1.47

Alternative name(s):
GDP-D-mannose dehydratase
Short name=GMD

Gene names

Name:	GMDS
Synonyms:	GMD

Organism

Cricetulus griseus (Chinese hamster) (Cricetulus barabensis griseus)

Taxonomic identifier

10029 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Cricetidae › Cricetinae › Cricetulus

Protein attributes

Sequence length	372 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Conversion of GDP-D-mannose to GDP-4-keto-6-D-deoxymannose.
Catalytic activity	GDP-mannose = GDP-4-dehydro-6-deoxy-D-mannose + H2O.
Cofactor	NADP By similarity.
Pathway	Nucleotide-sugar biosynthesis; GDP-L-fucose biosynthesis via de novo pathway; GDP-L-fucose from GDP-alpha-D-mannose: step 1/2.
Sequence similarities	Belongs to the GDP-mannose 4,6-dehydratase family.

Ontologies

Keywords
Ligand	NADP
Molecular function	Lyase
PTM	Acetylation
Gene Ontology (GO)
Biological process	'de novo' GDP-L-fucose biosynthetic process Inferred from sequence or structural similarity. Source: UniProtKB GDP-mannose metabolic process Inferred from sequence or structural similarity. Source: UniProtKB Notch signaling pathway Inferred from sequence or structural similarity. Source: UniProtKB
Cellular component	intracellular Inferred from electronic annotation. Source: InterPro
Molecular function	GDP-mannose 4,6-dehydratase activity Inferred from sequence or structural similarity. Source: UniProtKB NADP+ binding Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed By similarity
	Chain	2 – 372	371	GDP-mannose 4,6 dehydratase		PRO_0000201704
Sites
	Active site	155	1	By similarity
	Active site	157	1	Nucleophile By similarity
	Active site	179	1	Nucleophile By similarity
	Binding site	183	1	NADP By similarity
Amino acid modifications
	Modified residue	2	1	N-acetylalanine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8K3X3 [UniParc]. Last modified October 1, 2002. Version 1. Checksum: D4E256DF97230D2E	FASTA	372	41,974
	10 20 30 40 50 60 MAHAPASCPS SRNSGDGDKG KPRKVALITG ITGQDGSYLA EFLLEKGYEV HGIVRRSSSF 70 80 90 100 110 120 NTGRIEHLYK NPQAHIEGNM KLHYGDLTDS TCLVKIINEV KPTEIYNLGA QSHVKISFDL 130 140 150 160 170 180 AEYTADVDGV GTLRLLDAIK TCGLINSVKF YQASTSELYG KVQEIPQKET TPFYPRSPYG 190 200 210 220 230 240 AAKLYAYWIV VNFREAYNLF AVNGILFNHE SPRRGANFVT RKISRSVAKI YLGQLECFSL 250 260 270 280 290 300 GNLDAKRDWG HAKDYVEAMW LMLQNDEPED FVIATGEVHS VREFVEKSFM HIGKTIVWEG 310 320 330 340 350 360 KNENEVGRCK ETGKIHVTVD LKYYRPTEVD FLQGDCSKAQ QKLNWKPRVA FDELVREMVQ 370 ADVELMRTNP NA

References

[1]	"Glycan-dependent regulation of GDP-mannose 4,6-dehydratase activity." Becker D.J., Smith P.L., Petryniak B., Kelly R.J., Myers J.T., Wu B., Wang P.G., Lowe J.B. Submitted (JUN-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF525364 mRNA. Translation: AAM91925.1.
RefSeq	NP_001233625.1. NM_001246696.1.
3D structure databases
ProteinModelPortal	Q8K3X3.
SMR	Q8K3X3. Positions 23-369.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	100689436.
Phylogenomic databases
HOVERGEN	HBG000727.
Family and domain databases
InterPro	IPR001509. Epimerase_deHydtase. IPR006368. GDP_Man_deHydtase. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
PANTHER	PTHR10366:SF32. GDP_mann_dehyd. 1 hit.
Pfam	PF01370. Epimerase. 1 hit. [Graphical view]
TIGRFAMs	TIGR01472. Gmd. 1 hit.
ProtoNet	Search...

Entry information

Entry name

GMDS_CRIGR

Accession

Primary (citable) accession number: Q8K3X3

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 7, 2005
Last sequence update:	October 1, 2002
Last modified:	January 25, 2012
This is version 43 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program