Reviewed, UniProtKB/Swiss-Prot Q8K3X3 (GMDS_CRIGR)

Names and origin

Protein names

Recommended name:
    GDP-mannose 4,6 dehydratase
    EC=4.2.1.47
Alternative name(s):
    GDP-D-mannose dehydratase
      Short name=GMD

Gene names

Name:	GMDS
Synonyms:	GMD

Organism

Cricetulus griseus (Chinese hamster)

Taxonomic identifier

10029 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Cricetidae › Cricetinae › Cricetulus

Protein attributes

Sequence length	372 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Conversion of GDP-D-mannose to GDP-4-keto-6-D-deoxymannose.
Catalytic activity	GDP-mannose = GDP-4-dehydro-6-deoxy-D-mannose + H2O.
Cofactor	NADP By similarity.
Pathway	Nucleotide-sugar biosynthesis; GDP-L-fucose biosynthesis via de novo pathway; GDP-L-fucose from GDP-alpha-D-mannose: step 1/2.
Sequence similarities	Belongs to the GDP-mannose 4,6-dehydratase family.

Ontologies

Keywords
Ligand	NADP
Molecular function	Lyase
Gene Ontology (GO)
Biological process	GDP-mannose metabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	intracellular Inferred from electronic annotation. Source: InterPro
Molecular function	GDP-mannose 4,6-dehydratase activity Inferred from electronic annotation. Source: EC coenzyme binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 372	372	GDP-mannose 4,6 dehydratase		PRO_0000201704
Sites
	Active site	155	1	By similarity
	Active site	157	1	Nucleophile By similarity
	Active site	179	1	Nucleophile By similarity
	Binding site	183	1	NADP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8K3X3-1 [UniParc]. Last modified October 1, 2002. Version 1. Checksum: D4E256DF97230D2E	FASTA	372	41,974
	10 20 30 40 50 60 MAHAPASCPS SRNSGDGDKG KPRKVALITG ITGQDGSYLA EFLLEKGYEV HGIVRRSSSF 70 80 90 100 110 120 NTGRIEHLYK NPQAHIEGNM KLHYGDLTDS TCLVKIINEV KPTEIYNLGA QSHVKISFDL 130 140 150 160 170 180 AEYTADVDGV GTLRLLDAIK TCGLINSVKF YQASTSELYG KVQEIPQKET TPFYPRSPYG 190 200 210 220 230 240 AAKLYAYWIV VNFREAYNLF AVNGILFNHE SPRRGANFVT RKISRSVAKI YLGQLECFSL 250 260 270 280 290 300 GNLDAKRDWG HAKDYVEAMW LMLQNDEPED FVIATGEVHS VREFVEKSFM HIGKTIVWEG 310 320 330 340 350 360 KNENEVGRCK ETGKIHVTVD LKYYRPTEVD FLQGDCSKAQ QKLNWKPRVA FDELVREMVQ 370 ADVELMRTNP NA

References

[1]	"Glycan-dependent regulation of GDP-mannose 4,6-dehydratase activity." Becker D.J., Smith P.L., Petryniak B., Kelly R.J., Myers J.T., Wu B., Wang P.G., Lowe J.B. Submitted (JUN-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF525364 mRNA. Translation: AAM91925.1.
3D structure databases
HSSP	HSSP built from PDB template 1DB3 based on UniProtKB P32054.
SMR	Q8K3X3. Positions 23-369.
ModBase	Search...
Phylogenomic databases
HOVERGEN	Q8K3X3.
Enzyme and pathway databases
BRENDA	4.2.1.47. 18.
Family and domain databases
InterPro	IPR001509. Epimerase_deHydtase. IPR006368. GDP_Man_deHydtase. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
PANTHER	PTHR10366:SF32. GDP_mann_dehyd. 1 hit.
Pfam	PF01370. Epimerase. 1 hit. [Graphical view]
TIGRFAMs	TIGR01472. gmd. 1 hit.
ProtoNet	Search...

Entry information

Entry name

GMDS_CRIGR

Accession

Primary (citable) accession number: Q8K3X3

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 7, 2005
Last sequence update:	October 1, 2002
Last modified:	September 22, 2009
This is version 33 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)