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Protein

Protein CASC3

Gene

Casc3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Core component of the splicing-dependent multiprotein exon junction complex (EJC) deposited at splice junctions on mRNAs. The EJC is a dynamic structure consisting of core proteins and several peripheral nuclear and cytoplasmic associated factors that join the complex only transiently either during EJC assembly or during subsequent mRNA metabolism. The EJC marks the position of the exon-exon junction in the mature mRNA for the gene expression machinery and the core components remain bound to spliced mRNAs throughout all stages of mRNA metabolism thereby influencing downstream processes including nuclear mRNA export, subcellular mRNA localization, translation efficiency and nonsense-mediated mRNA decay (NMD). Stimulates the ATPase and RNA-helicase activities of EIF4A3. Plays a role in the stress response by participating in cytoplasmic stress granules assembly and by favoring cell recovery following stress. Component of the dendritic ribonucleoprotein particles (RNPs) in hippocampal neurons. May play a role in mRNA transport. Binds spliced mRNA in sequence-independent manner, 20-24 nucleotides upstream of mRNA exon-exon junctions. Binds poly(G) and poly(U) RNA homopolymer (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

mRNA processing, mRNA splicing, mRNA transport, Nonsense-mediated mRNA decay, Stress response, Translation regulation, Transport

Keywords - Ligandi

RNA-binding

Enzyme and pathway databases

ReactomeiR-MMU-109688. Cleavage of Growing Transcript in the Termination Region.
R-MMU-159236. Transport of Mature mRNA derived from an Intron-Containing Transcript.
R-MMU-72163. mRNA Splicing - Major Pathway.
R-MMU-72187. mRNA 3'-end processing.
R-MMU-975957. Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC).

Names & Taxonomyi

Protein namesi
Recommended name:
Protein CASC3
Alternative name(s):
Cancer susceptibility candidate gene 3 protein homolog
Metastatic lymph node gene 51 protein homolog
Short name:
MLN 51 homolog
Protein barentsz
Short name:
Btz
Short name:
mBtz
Gene namesi
Name:Casc3
Synonyms:Mln51
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 11

Organism-specific databases

MGIiMGI:2179723. Casc3.

Subcellular locationi

  • Cytoplasmperinuclear region 1 Publication
  • Nucleus 1 Publication
  • Nucleus speckle By similarity

  • Note: Travels to the cytoplasm as part of the exon junction complex (EJC) bound to mRNA. Shuttles between the cytoplasm and the stress granules. More specifically found in nuclear intrachromatin granules clusters (IGC), also called nuclear speckles, which are storage compartments for nuclear proteins involved in mRNA processing. Colocalizes in nuclear speckles with MAGOH. Under stress condition, colocalizes with FMR1 and TIA1, but not MAGOH and RBM8A EJC core factors, in cytoplasmic stress granules, which contain stored mRNAs whose translation is stopped in response to stress (By similarity). Predominantly found in the perinuclear region. Shuttles between the nucleus and the cytoplasm in a CRM1-dependent manner.By similarity

GO - Cellular componenti

  • cytoplasm Source: MGI
  • exon-exon junction complex Source: MGI
  • intracellular ribonucleoprotein complex Source: MGI
  • nuclear speck Source: UniProtKB-SubCell
  • nucleus Source: MGI
  • perinuclear region of cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi464L → A: Accumulation in the nucleus. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000893251 – 698Protein CASC3Add BLAST698

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei34PhosphoserineCombined sources1
Modified residuei115PhosphoserineBy similarity1
Modified residuei145PhosphoserineCombined sources1
Modified residuei262PhosphoserineBy similarity1
Modified residuei263PhosphoserineCombined sources1
Modified residuei354PhosphothreonineBy similarity1
Modified residuei360PhosphoserineBy similarity1
Modified residuei370PhosphoserineBy similarity1
Modified residuei472PhosphoserineBy similarity1

Post-translational modificationi

ADP-ribosylated by tankyrase TNKS and TNKS2. Poly-ADP-ribosylated protein is recognized by RNF146, followed by ubiquitination (By similarity).By similarity
Ubiquitinated by RNF146 when poly-ADP-ribosylated, leading to its degradation.By similarity

Keywords - PTMi

ADP-ribosylation, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiQ8K3W3.
MaxQBiQ8K3W3.
PaxDbiQ8K3W3.
PeptideAtlasiQ8K3W3.
PRIDEiQ8K3W3.

PTM databases

iPTMnetiQ8K3W3.
PhosphoSitePlusiQ8K3W3.

Expressioni

Tissue specificityi

High levels in heart, brain, including hippocampus and cerebellum, liver, kidney and testis; lower levels in muscle, lung and spleen.1 Publication

Gene expression databases

BgeeiENSMUSG00000078676.
CleanExiMM_CASC3.
GenevisibleiQ8K3W3. MM.

Interactioni

Subunit structurei

Part of the mRNA splicing-dependent exon junction complex (EJC) complex; the core complex contains CASC3, EIF4A3, MAGOH and RBM8A. Forms homooligomers. Interacts with STAU in an RNA-dependent manner. Interacts with EIF4A3, MAGOH, NXF1 and RBM8A.1 Publication

GO - Molecular functioni

Protein-protein interaction databases

BioGridi228651. 2 interactors.
STRINGi10090.ENSMUSP00000017384.

Structurei

3D structure databases

ProteinModelPortaliQ8K3W3.
SMRiQ8K3W3.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni134 – 280Necessary for RNA-binding, interaction with MAGOH and localization in nucleus specklesBy similarityAdd BLAST147
Regioni134 – 280Sufficient to form the EJCBy similarityAdd BLAST147
Regioni374 – 698Necessary for localization in cytoplasmic stress granulesBy similarityAdd BLAST325

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili98 – 127Sequence analysisAdd BLAST30

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi201 – 207Nuclear localization signal 1Sequence analysis7
Motifi251 – 259Nuclear localization signal 2Sequence analysis9
Motifi457 – 466Nuclear export signal10

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi40 – 45Poly-Gly6
Compositional biasi638 – 642Poly-Pro5
Compositional biasi686 – 689Poly-Pro4

Domaini

The coiled coil domain may be involved in oligomerization.By similarity

Sequence similaritiesi

Belongs to the CASC3 family.Curated

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiKOG4264. Eukaryota.
ENOG410ZW5Y. LUCA.
GeneTreeiENSGT00390000006930.
HOGENOMiHOG000069997.
HOVERGENiHBG050799.
InParanoidiQ8K3W3.
KOiK14323.
OMAiNLTEQNW.
OrthoDBiEOG091G0JLP.
PhylomeDBiQ8K3W3.
TreeFamiTF329663.

Family and domain databases

InterProiIPR018545. Btz_dom.
IPR028544. CASC3.
[Graphical view]
PANTHERiPTHR13434. PTHR13434. 1 hit.
PfamiPF09405. Btz. 1 hit.
[Graphical view]
SMARTiSM01044. Btz. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8K3W3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MADRRRQRAS QDTEDEESGA SGSDSGSPAR GGGSCSGSVG GGGSGSLPSQ
60 70 80 90 100
RGGRGGGLHL RRVESGGAKS AEESECESED GMEGDAVLSD YESAEDSEGE
110 120 130 140 150
EDYSEEENSK VELKSEANDA ADSSAKEKGE EKPESKGTVT GERQSGDGQE
160 170 180 190 200
STEPVENKVG KKGPKHLDDD EDRKNPAYIP RKGLFFEHDL RGQTQEEEVR
210 220 230 240 250
PKGRQRKLWK DEGRWEHDKF REDEQAPKSR QELIALYGYD IRSAHNPDDI
260 270 280 290 300
KPRRIRKPRF GSSPQRDPNW IGDRSSKSHR HQGPGGNLPP RTFINRNTAG
310 320 330 340 350
TGRMSASRNY SRSGGFKDGR TSFRPVEVAG QHGGRSAETL KHEASYRSRR
360 370 380 390 400
LEQTPVRDPS PEPDAPLLGS PEKEEVASET PAAVPDITPP APDRPIEKKS
410 420 430 440 450
YSRARRTRTK VGDAVKAAEE VPPPSEGLAS TATVPETTPA AKTGNWEAPV
460 470 480 490 500
DSTTGGLEQD VAQLNIAEQS WSPSQPSFLQ PRELRGVPNH IHMGAGPPPQ
510 520 530 540 550
FNRMEEMGVQ SGRAKRYSSQ RQRPVPEPPA PPVHISIMEG HYYDPLQFQG
560 570 580 590 600
PIYTHGDSPA PLPPQGMIVQ PEMHLPHPGL HPHQSPGPLP NPGLYPPPVS
610 620 630 640 650
MSPGQPPPQQ LLAPTYFSAP GVMNFGNPNY PYAPGALPPP PPPHLYPNTQ
660 670 680 690
APPQVYGGVT YYNPAQQQVQ PKPSPPRRTP QPVSIKPPPP EVVSRGSS
Length:698
Mass (Da):75,770
Last modified:July 7, 2009 - v3
Checksum:i3B718FC297A5E0F3
GO

Sequence cautioni

The sequence AAH60672 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti297N → D in BAE24081 (PubMed:16141072).Curated1
Sequence conflicti474S → G in AAH34533 (PubMed:15489334).Curated1
Sequence conflicti589L → V in BAE24081 (PubMed:16141072).Curated1
Sequence conflicti638Missing in CAC27775 (PubMed:12843282).Curated1
Sequence conflicti638Missing in AAH60672 (PubMed:15489334).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF526276 mRNA. Translation: AAM88396.1.
AJ292072 mRNA. Translation: CAC27775.1.
AK139608 mRNA. Translation: BAE24081.1.
AL590963 Genomic DNA. Translation: CAM46181.1.
CH466556 Genomic DNA. Translation: EDL16170.1.
BC034533 mRNA. Translation: AAH34533.1.
BC060672 mRNA. Translation: AAH60672.1. Different initiation.
BC141296 mRNA. Translation: AAI41297.1.
CCDSiCCDS36302.1.
RefSeqiNP_619601.2. NM_138660.2.
UniGeneiMm.40120.

Genome annotation databases

EnsembliENSMUST00000017384; ENSMUSP00000017384; ENSMUSG00000078676.
ENSMUST00000169695; ENSMUSP00000130926; ENSMUSG00000078676.
GeneIDi192160.
KEGGimmu:192160.
UCSCiuc007lhq.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF526276 mRNA. Translation: AAM88396.1.
AJ292072 mRNA. Translation: CAC27775.1.
AK139608 mRNA. Translation: BAE24081.1.
AL590963 Genomic DNA. Translation: CAM46181.1.
CH466556 Genomic DNA. Translation: EDL16170.1.
BC034533 mRNA. Translation: AAH34533.1.
BC060672 mRNA. Translation: AAH60672.1. Different initiation.
BC141296 mRNA. Translation: AAI41297.1.
CCDSiCCDS36302.1.
RefSeqiNP_619601.2. NM_138660.2.
UniGeneiMm.40120.

3D structure databases

ProteinModelPortaliQ8K3W3.
SMRiQ8K3W3.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi228651. 2 interactors.
STRINGi10090.ENSMUSP00000017384.

PTM databases

iPTMnetiQ8K3W3.
PhosphoSitePlusiQ8K3W3.

Proteomic databases

EPDiQ8K3W3.
MaxQBiQ8K3W3.
PaxDbiQ8K3W3.
PeptideAtlasiQ8K3W3.
PRIDEiQ8K3W3.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000017384; ENSMUSP00000017384; ENSMUSG00000078676.
ENSMUST00000169695; ENSMUSP00000130926; ENSMUSG00000078676.
GeneIDi192160.
KEGGimmu:192160.
UCSCiuc007lhq.1. mouse.

Organism-specific databases

CTDi22794.
MGIiMGI:2179723. Casc3.

Phylogenomic databases

eggNOGiKOG4264. Eukaryota.
ENOG410ZW5Y. LUCA.
GeneTreeiENSGT00390000006930.
HOGENOMiHOG000069997.
HOVERGENiHBG050799.
InParanoidiQ8K3W3.
KOiK14323.
OMAiNLTEQNW.
OrthoDBiEOG091G0JLP.
PhylomeDBiQ8K3W3.
TreeFamiTF329663.

Enzyme and pathway databases

ReactomeiR-MMU-109688. Cleavage of Growing Transcript in the Termination Region.
R-MMU-159236. Transport of Mature mRNA derived from an Intron-Containing Transcript.
R-MMU-72163. mRNA Splicing - Major Pathway.
R-MMU-72187. mRNA 3'-end processing.
R-MMU-975957. Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC).

Miscellaneous databases

PROiQ8K3W3.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000078676.
CleanExiMM_CASC3.
GenevisibleiQ8K3W3. MM.

Family and domain databases

InterProiIPR018545. Btz_dom.
IPR028544. CASC3.
[Graphical view]
PANTHERiPTHR13434. PTHR13434. 1 hit.
PfamiPF09405. Btz. 1 hit.
[Graphical view]
SMARTiSM01044. Btz. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCASC3_MOUSE
AccessioniPrimary (citable) accession number: Q8K3W3
Secondary accession number(s): A3KFP7
, Q3UT99, Q8K219, Q99NF0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 13, 2004
Last sequence update: July 7, 2009
Last modified: November 30, 2016
This is version 117 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.