Reviewed, UniProtKB/Swiss-Prot Q8K3W3 (CASC3_MOUSE)

Names and origin

Protein names

Recommended name:
    Protein CASC3
Alternative name(s):
    Cancer susceptibility candidate gene 3 protein homolog
    Metastatic lymph node protein 51 homolog
      Short name=Protein MLN 51 homolog
    Protein barentsz
      Short name=Btz

Gene names

Name:	Casc3
Synonyms:	Mln51

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	697 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Component of the dendritic ribonucleoprotein particles (RNPs) in hippocampal neurons. May play a role in mRNA transport.
Subunit structure	Interacts with STAU in an RNA-dependent manner. Forms homo-oligomers By similarity.
Subcellular location	Cytoplasm › perinuclear region. Nucleus. Note: Predominantly found in the perinuclear region. Shuttles between the nucleus and the cytoplasm in a CRM1-dependent manner. Ref.2
Tissue specificity	High levels in heart, brain, including hippocampus and cerebellum, liver, kidney and testis; lower levels in muscle, lung and spleen.
Domain	The coiled coil domain may be involved in oligomerization By similarity.
Post-translational modification	Phosphorylated By similarity.

Ontologies

Keywords
Biological process	Transport mRNA transport
Cellular component	Cytoplasm Nucleus
Domain	Coiled coil
PTM	Phosphoprotein
Gene Ontology (GO)
Biological process	intracellular mRNA localization Ref.2 Inferred from direct assay. Source: MGI mRNA transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	nucleus Ref.2 Inferred from direct assay. Source: MGI perinuclear region of cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell ribonucleoprotein complex Ref.2 Inferred from direct assay. Source: MGI
Molecular function	protein binding Ref.2 Inferred from physical interaction. Source: MGI
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 697	697	Protein CASC3		PRO_0000089325
Regions
	Coiled coil	98 – 127	30	Potential
	Motif	201 – 207	7	Nuclear localization signal 1 Potential
	Motif	251 – 259	9	Nuclear localization signal 2 Potential
	Motif	457 – 466	10	Nuclear export signal
	Compositional bias	40 – 45	6	Poly-Gly
	Compositional bias	638 – 642	5	Poly-Pro
	Compositional bias	686 – 689	4	Poly-Pro
Amino acid modifications
	Modified residue	145	1	Phosphoserine
	Modified residue	263	1	Phosphoserine Ref.4
	Modified residue	360	1	Phosphoserine By similarity
	Modified residue	370	1	Phosphoserine By similarity
	Modified residue	472	1	Phosphoserine By similarity
Experimental info
	Mutagenesis	464	1	L → A: Accumulation in the nucleus. Ref.2
	Sequence conflict	474	1	S → G in AAH34533. Ref.3
	Sequence conflict	642	1	P → PP in AAM88396. Ref.1
	Sequence conflict	642	1	P → PP in AAH34533. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	Q8K3W3-1 [UniParc]. Last modified April 13, 2004. Version 2. Checksum: C230605C23D47D05	FASTA	697	75,673
	10 20 30 40 50 60 MADRRRQRAS QDTEDEESGA SGSDSGSPAR GGGSCSGSVG GGGSGSLPSQ RGGRGGGLHL 70 80 90 100 110 120 RRVESGGAKS AEESECESED GMEGDAVLSD YESAEDSEGE EDYSEEENSK VELKSEANDA 130 140 150 160 170 180 ADSSAKEKGE EKPESKGTVT GERQSGDGQE STEPVENKVG KKGPKHLDDD EDRKNPAYIP 190 200 210 220 230 240 RKGLFFEHDL RGQTQEEEVR PKGRQRKLWK DEGRWEHDKF REDEQAPKSR QELIALYGYD 250 260 270 280 290 300 IRSAHNPDDI KPRRIRKPRF GSSPQRDPNW IGDRSSKSHR HQGPGGNLPP RTFINRNTAG 310 320 330 340 350 360 TGRMSASRNY SRSGGFKDGR TSFRPVEVAG QHGGRSAETL KHEASYRSRR LEQTPVRDPS 370 380 390 400 410 420 PEPDAPLLGS PEKEEVASET PAAVPDITPP APDRPIEKKS YSRARRTRTK VGDAVKAAEE 430 440 450 460 470 480 VPPPSEGLAS TATVPETTPA AKTGNWEAPV DSTTGGLEQD VAQLNIAEQS WSPSQPSFLQ 490 500 510 520 530 540 PRELRGVPNH IHMGAGPPPQ FNRMEEMGVQ SGRAKRYSSQ RQRPVPEPPA PPVHISIMEG 550 560 570 580 590 600 HYYDPLQFQG PIYTHGDSPA PLPPQGMIVQ PEMHLPHPGL HPHQSPGPLP NPGLYPPPVS 610 620 630 640 650 660 MSPGQPPPQQ LLAPTYFSAP GVMNFGNPNY PYAPGALPPP PPHLYPNTQA PPQVYGGVTY 670 680 690 YNPAQQQVQP KPSPPRRTPQ PVSIKPPPPE VVSRGSS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Metastatic lymph node 51, a novel nucleo-cytoplasmic protein overexpressed in breast cancer." Degot S.F., Regnier C.H., Wendling C., Chenard M.-P., Rio M.-C., Tomasetto C.L. Oncogene 21:4422-4434(2002) [PubMed: 12080473] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Barentsz, a new component of the Staufen-containing ribonucleoprotein particles in mammalian cells, interacts with Staufen in an RNA-dependent manner." Macchi P., Kroening S., Palacios I.M., Baldassa S., Grunewald B., Ambrosino C., Goetze B., Lupas A., St Johnston D., Kiebler M. J. Neurosci. 23:5778-5788(2003) [PubMed: 12843282] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], SUBCELLULAR LOCATION, MUTAGENESIS OF LEU-464. Strain: BALB/c.
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 40-697. Strain: Czech II and FVB/N. Tissue: Kidney and Mammary tumor.
[4]	"Large-scale phosphorylation analysis of mouse liver." Villen J., Beausoleil S.A., Gerber S.A., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 104:1488-1493(2007) [PubMed: 17242355] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-263, MASS SPECTROMETRY. Tissue: Liver.
[5]	"Large scale localization of protein phosphorylation by use of electron capture dissociation mass spectrometry." Sweet S.M., Bailey C.M., Cunningham D.L., Heath J.K., Cooper H.J. Mol. Cell. Proteomics 8:904-912(2009) [PubMed: 19131326] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-145, MASS SPECTROMETRY.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF526276 mRNA. Translation: AAM88396.1. AJ292072 mRNA. Translation: CAC27775.1. BC034533 mRNA. Translation: AAH34533.1. BC060672 mRNA. Translation: AAH60672.1. Different initiation.
IPI	IPI00877339.
UniGene	Mm.40120
3D structure databases
SMR	Q8K3W3. Positions 165-245.
ModBase	Search...
PTM databases
PhosphoSite	Q8K3W3.
Genome annotation databases
Ensembl	ENSMUSG00000078676. Mus musculus. [Contig view]
Organism-specific databases
MGI	MGI:2179723. Casc3.
Phylogenomic databases
HOVERGEN	Q8K3W3.
Gene expression databases
ArrayExpress	Q8K3W3.
Bgee	Q8K3W3.
CleanEx	MM_CASC3.
GermOnline	ENSMUSG00000052915. Mus musculus.
Family and domain databases
InterPro	IPR018545. CASC3/Barentsz_eIF4AIII-bd. [Graphical view]
Pfam	PF09405. Btz. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
SOURCE	Search...

Entry information

Entry name

CASC3_MOUSE

Accession

Primary (citable) accession number: Q8K3W3
Secondary accession number(s): Q8K219, Q99NF0

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 13, 2004
Last sequence update:	April 13, 2004
Last modified:	June 16, 2009
This is version 53 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)