Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Protein-arginine deiminase type-6

Gene

Padi6

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the deimination of arginine residues of proteins (By similarity). May be involved in cytoskeletal reorganization in the egg and early embryo.By similarity

Catalytic activityi

Protein L-arginine + H2O = protein L-citrulline + NH3.

Cofactori

Ca2+By similarity

GO - Molecular functioni

  • calcium ion binding Source: InterPro
  • protein-arginine deiminase activity Source: MGI

GO - Biological processi

  • cellular protein localization Source: MGI
  • cytoplasm organization Source: MGI
  • cytoskeleton organization Source: MGI
  • protein citrullination Source: MGI
  • regulation of translation by machinery localization Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Ligandi

Calcium

Enzyme and pathway databases

BRENDAi3.5.3.15. 3474.

Names & Taxonomyi

Protein namesi
Recommended name:
Protein-arginine deiminase type-6 (EC:3.5.3.15)
Alternative name(s):
Arginine deiminase-like protein
Egg and embryo abundant PAD
Short name:
ePAD
Peptidylarginine deiminase VI
Protein-arginine deiminase type VI
Gene namesi
Name:Padi6
Synonyms:Pad6, Padi5
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 4

Organism-specific databases

MGIiMGI:2655198. Padi6.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: MGI
  • intermediate filament cytoskeleton Source: MGI
  • nucleus Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 682682Protein-arginine deiminase type-6PRO_0000220037Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21PhosphoserineBy similarity
Modified residuei434 – 4341PhosphoserineBy similarity

Post-translational modificationi

Phosphorylation at Ser-2, possibly by RSK-type kinases, and Ser-434 creates binding sites for 14-3-3 proteins.By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ8K3V4.
PaxDbiQ8K3V4.
PRIDEiQ8K3V4.

2D gel databases

REPRODUCTION-2DPAGEQ8K3V4.

PTM databases

PhosphoSiteiQ8K3V4.

Expressioni

Tissue specificityi

Expressed at very high levels in oocytes. Weakly expressed in testis. Expressed in primordial, primary, secondary and Graafian follicles, and in immature oocytes, mature eggs and blastocyst (at protein level).1 Publication

Developmental stagei

Detected in developing oocytes and early embryo. Detected in immature-germinal vesicle-stage oocytes, mature metaphase II arrested eggs and pronuclear zygotes, 2-cell, 4-cell and morula stages. Expression decreases in blastocyst stage.1 Publication

Gene expression databases

BgeeiENSMUSG00000040935.
CleanExiMM_PADI6.
ExpressionAtlasiQ8K3V4. baseline and differential.
GenevisibleiQ8K3V4. MM.

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000044044.

Structurei

3D structure databases

ProteinModelPortaliQ8K3V4.
SMRiQ8K3V4. Positions 14-682.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the protein arginine deiminase family.Curated

Phylogenomic databases

eggNOGiENOG410IF3F. Eukaryota.
ENOG410ZKF3. LUCA.
GeneTreeiENSGT00390000008680.
HOGENOMiHOG000220908.
HOVERGENiHBG053016.
InParanoidiQ8K3V4.
KOiK01481.
OMAiPLEVYLC.
OrthoDBiEOG091G02QG.
PhylomeDBiQ8K3V4.
TreeFamiTF331952.

Family and domain databases

InterProiIPR008972. Cupredoxin.
IPR004303. PAD.
IPR013530. PAD_C.
IPR013732. PAD_N.
IPR013733. Prot_Arg_deaminase_cen_dom.
[Graphical view]
PANTHERiPTHR10837. PTHR10837. 1 hit.
PfamiPF03068. PAD. 1 hit.
PF08527. PAD_M. 1 hit.
PF08526. PAD_N. 1 hit.
[Graphical view]
PIRSFiPIRSF001247. Protein-arginine_deiminase. 1 hit.
SUPFAMiSSF110083. SSF110083. 1 hit.
SSF49503. SSF49503. 1 hit.

Sequencei

Sequence statusi: Complete.

Q8K3V4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSFQNSLSLS LVNPTHALCM VGMEITLDIS KCAPDKCKSF TIRGSPRILI
60 70 80 90 100
HISSSVIAGK EDTVVWRSMN HPTVALVRMV APSPTVDEDK VLVSYFCPDQ
110 120 130 140 150
EVPTATAVLF LTGIEISLEA DIYRDGQLDM PSDKQAKKKW MWGMNGWGAI
160 170 180 190 200
LLVNCSPNAV GQPDEQSFQE GPREIQNLSQ MNVTVEGPTS ILQNYQLILH
210 220 230 240 250
TSEEEAKKTR VYWSQRGSSA YELVVGPNKP VYLLPTFENR RKEAFYVEAT
260 270 280 290 300
EFPSPSFSGL ISLSLSLVEK AHDECIPEIP LYKDTVMFRV APYIFMPSTQ
310 320 330 340 350
MPLEVYLCRE LQLQGFVDSV TKLSEKSKVQ VVKVYEDPNR QSKWLQDEMA
360 370 380 390 400
FCYTQAPHKT VSLILDTPRV SKLEDFPMKY TLTPGSGYLI RQTEDHRVAS
410 420 430 440 450
LDSIGNLMVS PPVKAQGKDY PLGRVLIGGS FYPSSEGRDM NKGLREFVYA
460 470 480 490 500
QQVQAPVELF SDWLMTGHMD QFMCFVPTND KNNDQKDFRL LLASPSACFE
510 520 530 540 550
LFEQKQKEGY GNVTLFEDIG AEQLLSNGRE SKTISQILAD KSFREQNTYV
560 570 580 590 600
EKCISLNRTL LKTELGLEDK DIILIPQLFC LEQLTNVPSN QQSTKLFARP
610 620 630 640 650
YFPDMLQIIV LGKNLGIPKP FGPKINGTCC LEEKVCGLLE PLGLKCTFID
660 670 680
DFDCYLANIG DVCASAIINR VPFAFKWWKM TP
Length:682
Mass (Da):76,778
Last modified:December 21, 2004 - v2
Checksum:i8B9A0371C40BA216
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti63 – 631T → A in AAH53724 (PubMed:15489334).Curated
Sequence conflicti122 – 1221I → T in AAH53724 (PubMed:15489334).Curated
Sequence conflicti177 – 1771N → NN AA sequence (PubMed:12654293).Curated
Sequence conflicti393 – 3931T → I in AAM94858 (PubMed:12654293).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF529423 mRNA. Translation: AAM94858.1.
AB121692 Genomic DNA. Translation: BAD16628.1.
BC053724 mRNA. Translation: AAH53724.1.
CCDSiCCDS38936.1.
RefSeqiNP_694746.2. NM_153106.2.
UniGeneiMm.271661.

Genome annotation databases

EnsembliENSMUST00000038749; ENSMUSP00000044044; ENSMUSG00000040935.
GeneIDi242726.
KEGGimmu:242726.
UCSCiuc008vnc.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF529423 mRNA. Translation: AAM94858.1.
AB121692 Genomic DNA. Translation: BAD16628.1.
BC053724 mRNA. Translation: AAH53724.1.
CCDSiCCDS38936.1.
RefSeqiNP_694746.2. NM_153106.2.
UniGeneiMm.271661.

3D structure databases

ProteinModelPortaliQ8K3V4.
SMRiQ8K3V4. Positions 14-682.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000044044.

PTM databases

PhosphoSiteiQ8K3V4.

2D gel databases

REPRODUCTION-2DPAGEQ8K3V4.

Proteomic databases

MaxQBiQ8K3V4.
PaxDbiQ8K3V4.
PRIDEiQ8K3V4.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000038749; ENSMUSP00000044044; ENSMUSG00000040935.
GeneIDi242726.
KEGGimmu:242726.
UCSCiuc008vnc.1. mouse.

Organism-specific databases

CTDi353238.
MGIiMGI:2655198. Padi6.

Phylogenomic databases

eggNOGiENOG410IF3F. Eukaryota.
ENOG410ZKF3. LUCA.
GeneTreeiENSGT00390000008680.
HOGENOMiHOG000220908.
HOVERGENiHBG053016.
InParanoidiQ8K3V4.
KOiK01481.
OMAiPLEVYLC.
OrthoDBiEOG091G02QG.
PhylomeDBiQ8K3V4.
TreeFamiTF331952.

Enzyme and pathway databases

BRENDAi3.5.3.15. 3474.

Miscellaneous databases

PROiQ8K3V4.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000040935.
CleanExiMM_PADI6.
ExpressionAtlasiQ8K3V4. baseline and differential.
GenevisibleiQ8K3V4. MM.

Family and domain databases

InterProiIPR008972. Cupredoxin.
IPR004303. PAD.
IPR013530. PAD_C.
IPR013732. PAD_N.
IPR013733. Prot_Arg_deaminase_cen_dom.
[Graphical view]
PANTHERiPTHR10837. PTHR10837. 1 hit.
PfamiPF03068. PAD. 1 hit.
PF08527. PAD_M. 1 hit.
PF08526. PAD_N. 1 hit.
[Graphical view]
PIRSFiPIRSF001247. Protein-arginine_deiminase. 1 hit.
SUPFAMiSSF110083. SSF110083. 1 hit.
SSF49503. SSF49503. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiPADI6_MOUSE
AccessioniPrimary (citable) accession number: Q8K3V4
Secondary accession number(s): Q75WC6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 4, 2003
Last sequence update: December 21, 2004
Last modified: September 7, 2016
This is version 105 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.