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Protein

G-protein coupled receptor 56

Gene

Gpr56

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in cell adhesion and probably in cell-cell interactions. Regulates the migration of neural precursor cells. Receptor for collagen III/Col3a1 in the developing brain and involved in regulation of cortical development, specifically in maintenance of the pial basemant membrane integrity and in cortical lamination. Binding to the Col3a1 ligand inhibits neuronal migration and activates the RhoA pathway by coupling to Gna13 and possibly Gna12 (By similarity). Required for normal cortical development and regulation of neuroprogenitor cells proliferation (By similarity).By similarity

Enzyme regulationi

GPR56 NT is proposed to inhibit receptor signaling; its interactions with extracellular ligands and /or homophilic GPR56 NT interactions may relieve the inhibition.By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Developmental protein, G-protein coupled receptor, Receptor, Transducer

Keywords - Biological processi

Cell adhesion, Differentiation, Neurogenesis

Protein family/group databases

MEROPSiP02.008.

Names & Taxonomyi

Protein namesi
Recommended name:
G-protein coupled receptor 56
Cleaved into the following 2 chains:
GPR56 N-terminal fragment
Short name:
GPR56 NT
Short name:
GPR56(N)
Alternative name(s):
GPR56 extracellular subunit
GPR56 subunit alpha
GPR56 C-terminal fragment
Short name:
GPR56 CT
Short name:
GPR56(C)
Alternative name(s):
GPR56 seven-transmembrane subunit
Short name:
GPR56 7TM
GPR56 subunit beta
Gene namesi
Name:Gpr56
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi628617. Gpr56.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini26 – 402377ExtracellularSequence analysisAdd
BLAST
Transmembranei403 – 42321Helical; Name=1Sequence analysisAdd
BLAST
Topological domaini424 – 44219CytoplasmicSequence analysisAdd
BLAST
Transmembranei443 – 46321Helical; Name=2Sequence analysisAdd
BLAST
Topological domaini464 – 4718ExtracellularSequence analysis
Transmembranei472 – 49221Helical; Name=3Sequence analysisAdd
BLAST
Topological domaini493 – 51220CytoplasmicSequence analysisAdd
BLAST
Transmembranei513 – 53321Helical; Name=4Sequence analysisAdd
BLAST
Topological domaini534 – 57037ExtracellularSequence analysisAdd
BLAST
Transmembranei571 – 59121Helical; Name=5Sequence analysisAdd
BLAST
Topological domaini592 – 60312CytoplasmicSequence analysisAdd
BLAST
Transmembranei604 – 62421Helical; Name=6Sequence analysisAdd
BLAST
Topological domaini625 – 6306ExtracellularSequence analysis
Transmembranei631 – 65121Helical; Name=7Sequence analysisAdd
BLAST
Topological domaini652 – 68736CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2525Sequence analysisAdd
BLAST
Chaini26 – 687662G-protein coupled receptor 56PRO_0000012885Add
BLAST
Chaini26 – ?382357GPR56 N-terminal fragmentPRO_0000423096Add
BLAST
Chaini?383 – 687305GPR56 C-terminal fragmentPRO_0000423097Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi39 – 391N-linked (GlcNAc...)Sequence analysis
Glycosylationi148 – 1481N-linked (GlcNAc...)Sequence analysis
Glycosylationi171 – 1711N-linked (GlcNAc...)Sequence analysis
Glycosylationi234 – 2341N-linked (GlcNAc...)Sequence analysis
Glycosylationi303 – 3031N-linked (GlcNAc...)Sequence analysis
Glycosylationi324 – 3241N-linked (GlcNAc...)Sequence analysis
Glycosylationi341 – 3411N-linked (GlcNAc...)Sequence analysis

Post-translational modificationi

Autoproteolytically cleaved into 2 fragments; the large extracellular N-terminal fragment and the membrane-bound C-terminal fragment predominantly remain associated and non-covalently linked. Shedding to yield the secreted GPR56 N-terminal fragment seems also to involve metalloprotease(s).1 Publication
N-glycosylated. Contains sialic acid residues (By similarity).By similarity
Ubiquitinated. Undergoes polyubiquitination upon activation (By similarity).By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei382 – 3832Cleavage; by autolysisBy similarity

Keywords - PTMi

Glycoprotein, Ubl conjugation

Proteomic databases

PaxDbiQ8K3V3.
PRIDEiQ8K3V3.

PTM databases

PhosphoSiteiQ8K3V3.
UniCarbKBiQ8K3V3.

Interactioni

Subunit structurei

Predominantly non-covalently linked heterodimer of the N-terminal and the C-terminal fragment. GPR56 NT self-associates in a trans-trans manner; the homophilic interaction enhances receptor signaling. GPR56 CT interacts with ARRB2; the interaction is impaired by GPR56 NT. Part of a GPCR-tetraspanin complex at least consisting of GPR56, CD81, eventually CD9, and GNA11 in which CD81 is enhancing the association of GPR56 with GNA11 (By similarity).By similarity

GO - Molecular functioni

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000020921.

Structurei

3D structure databases

ProteinModelPortaliQ8K3V3.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini343 – 39452GPSPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 GPS domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG4193. Eukaryota.
ENOG410XSD2. LUCA.
HOGENOMiHOG000015136.
HOVERGENiHBG051814.
InParanoidiQ8K3V3.
PhylomeDBiQ8K3V3.

Family and domain databases

InterProiIPR017981. GPCR_2-like.
IPR000832. GPCR_2_secretin-like.
IPR003910. GPR56/AGRG3/AGRG5.
IPR000203. GPS.
[Graphical view]
PfamiPF00002. 7tm_2. 1 hit.
PF01825. GPS. 1 hit.
[Graphical view]
PRINTSiPR00249. GPCRSECRETIN.
PR01422. GPR56ORPHANR.
SMARTiSM00303. GPS. 1 hit.
[Graphical view]
PROSITEiPS50261. G_PROTEIN_RECEP_F2_4. 1 hit.
PS50221. GPS. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q8K3V3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAVQVLLQMV YFLLTLLFLV QGAHGASPRE DFRFCGQRNQ TQQSTLHYDQ
60 70 80 90 100
TSEPHIFVWN TDESLTIRAP FPAAPDIPYF FPEPRGLYHF CLYWSRHTGR
110 120 130 140 150
LHLRYGKNDY LLSSRASNLL CYRKQEESLK QGAPLVATSV SSWQSPQNTS
160 170 180 190 200
LPGAPSFIFS FHNAPHKVSH NASVNMCDLK KELQLLSKFL QHPHKASKRP
210 220 230 240 250
SAAFISQQLQ NLESKLTSVS FLGDTLSFEE NRVNATVWKL PPTAGLEDLQ
260 270 280 290 300
IHSQQEEEQS EVQAYSVLLP RAVFQQTRGR RRDAAKRLLV VDFSSQALFQ
310 320 330 340 350
DKNSSQVLGE KVLGIVVQNT KVTNLSDPVV LTFQHQPQPK NVTLQCVFWV
360 370 380 390 400
EDPASSSTGS WSSEGCETVS RDTQTSCLCN HLTYFAVLMV SSMEVEATHK
410 420 430 440 450
HYLTLLSYVG CVISALACVF TIAAYLCTRR KSRDYTIKVH MNLLLAVFLL
460 470 480 490 500
DVSFLLSEPV ALMGSEAACR TSAMFLHFSL LACLSWMGLE GYNLYRLVVE
510 520 530 540 550
VFGTYVPGYL LKLSTVGWGF PVFLVTLVAL VDVNNYGPII LAVRRTPDHV
560 570 580 590 600
IYPSMCWIRD SVVSYVTNLG LFSLVFLFNM AMLATMVVQI LRLRPHSQKW
610 620 630 640 650
PHVLTLLGLS LVLGLPWALV FFSFASGTFQ LVIIYLFSIM TSFQGFLIFL
660 670 680
WYWSMRFQAQ GGPSPLKNNS DSAKLPISSG STSSSRI
Length:687
Mass (Da):77,252
Last modified:October 1, 2002 - v1
Checksum:i79C8A64FC572856D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF529886 mRNA. Translation: AAM94855.1.
UniGeneiRn.1677.

Genome annotation databases

UCSCiRGD:628617. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF529886 mRNA. Translation: AAM94855.1.
UniGeneiRn.1677.

3D structure databases

ProteinModelPortaliQ8K3V3.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000020921.

Protein family/group databases

MEROPSiP02.008.
GPCRDBiSearch...

PTM databases

PhosphoSiteiQ8K3V3.
UniCarbKBiQ8K3V3.

Proteomic databases

PaxDbiQ8K3V3.
PRIDEiQ8K3V3.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

UCSCiRGD:628617. rat.

Organism-specific databases

RGDi628617. Gpr56.

Phylogenomic databases

eggNOGiKOG4193. Eukaryota.
ENOG410XSD2. LUCA.
HOGENOMiHOG000015136.
HOVERGENiHBG051814.
InParanoidiQ8K3V3.
PhylomeDBiQ8K3V3.

Miscellaneous databases

PROiQ8K3V3.

Family and domain databases

InterProiIPR017981. GPCR_2-like.
IPR000832. GPCR_2_secretin-like.
IPR003910. GPR56/AGRG3/AGRG5.
IPR000203. GPS.
[Graphical view]
PfamiPF00002. 7tm_2. 1 hit.
PF01825. GPS. 1 hit.
[Graphical view]
PRINTSiPR00249. GPCRSECRETIN.
PR01422. GPR56ORPHANR.
SMARTiSM00303. GPS. 1 hit.
[Graphical view]
PROSITEiPS50261. G_PROTEIN_RECEP_F2_4. 1 hit.
PS50221. GPS. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Inhibition of rat serpentine receptor mRNA expression by leptin in insulin producing pancreatic beta-cells."
    Kuehnen P., Seufert J.
    Submitted (JUL-2002) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "The N terminus of the adhesion G protein-coupled receptor GPR56 controls receptor signaling activity."
    Paavola K.J., Stephenson J.R., Ritter S.L., Alter S.P., Hall R.A.
    J. Biol. Chem. 286:28914-28921(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBUNIT, PROTEOLYTIC PROCESSING.
  3. "Quantitative maps of protein phosphorylation sites across 14 different rat organs and tissues."
    Lundby A., Secher A., Lage K., Nordsborg N.B., Dmytriyev A., Lundby C., Olsen J.V.
    Nat. Commun. 3:876-876(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiGPR56_RAT
AccessioniPrimary (citable) accession number: Q8K3V3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 15, 2004
Last sequence update: October 1, 2002
Last modified: January 20, 2016
This is version 95 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. 7-transmembrane G-linked receptors
    List of 7-transmembrane G-linked receptor entries
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.