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Protein

Free fatty acid receptor 1

Gene

Ffar1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

G-protein coupled receptor for medium and long chain saturated and unsaturated fatty acids that plays an important role in glucose homeostasis. Fatty acid binding increases glucose-stimulated insulin secretion, and may also enhance the secretion of glucagon-like peptide 1 (GLP-1). May also play a role in bone homeostasis; receptor signaling activates pathways that inhibit osteoclast differentiation. Ligand binding leads to a conformation change that triggers signaling via G-proteins that activate phospholipase C, leading to an increase of the intracellular calcium concentration. Seems to act through a G(q) and G(i)-mediated pathway.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei145 – 1451Important for receptor activationBy similarity
Sitei172 – 1721Important for receptor activationBy similarity
Binding sitei183 – 1831AgonistBy similarity
Binding sitei240 – 2401AgonistBy similarity
Binding sitei258 – 2581AgonistBy similarity

GO - Molecular functioni

  • bioactive lipid receptor activity Source: UniProtKB
  • fatty acid binding Source: RGD

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

G-protein coupled receptor, Receptor, Transducer

Keywords - Ligandi

Lipid-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Free fatty acid receptor 1
Alternative name(s):
G-protein coupled receptor 40
Gene namesi
Name:Ffar1
Synonyms:Gpr40
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi628613. Ffar1.

Subcellular locationi

  • Cell membrane By similarity; Multi-pass membrane protein By similarity

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 88ExtracellularSequence analysisBy similarity
Transmembranei9 – 3123Helical; Name=1Sequence analysisBy similarityAdd
BLAST
Topological domaini32 – 4110CytoplasmicSequence analysisBy similarity
Transmembranei42 – 6423Helical; Name=2Sequence analysisBy similarityAdd
BLAST
Topological domaini65 – 7915ExtracellularSequence analysisBy similarityAdd
BLAST
Transmembranei80 – 10122Helical; Name=3Sequence analysisBy similarityAdd
BLAST
Topological domaini102 – 12120CytoplasmicSequence analysisBy similarityAdd
BLAST
Transmembranei122 – 14221Helical; Name=4Sequence analysisBy similarityAdd
BLAST
Topological domaini143 – 17836ExtracellularSequence analysisBy similarityAdd
BLAST
Transmembranei179 – 20022Helical; Name=5Sequence analysisBy similarityAdd
BLAST
Topological domaini201 – 22323CytoplasmicSequence analysisBy similarityAdd
BLAST
Transmembranei224 – 24825Helical; Name=6Sequence analysisBy similarityAdd
BLAST
Topological domaini249 – 2568ExtracellularSequence analysisBy similarity
Transmembranei257 – 27923Helical; Name=7Sequence analysisBy similarityAdd
BLAST
Topological domaini280 – 30021CytoplasmicSequence analysisBy similarityAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Chemistry

ChEMBLiCHEMBL1795180.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 300300Free fatty acid receptor 1PRO_0000227756Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi79 ↔ 170By similarity
Glycosylationi153 – 1531N-linked (GlcNAc...)Sequence analysis

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiQ8K3T4.

PTM databases

PhosphoSiteiQ8K3T4.

Expressioni

Tissue specificityi

Expressed abundantly in pancreatic beta cells.2 Publications

Interactioni

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000028533.

Chemistry

BindingDBiQ8K3T4.

Structurei

3D structure databases

ProteinModelPortaliQ8K3T4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni87 – 915Agonist bindingBy similarity

Sequence similaritiesi

Belongs to the G-protein coupled receptor 1 family.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IV8D. Eukaryota.
ENOG4111572. LUCA.
HOGENOMiHOG000236290.
HOVERGENiHBG080696.
InParanoidiQ8K3T4.
KOiK04325.
PhylomeDBiQ8K3T4.

Family and domain databases

InterProiIPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
IPR013312. GPR40-rel_orph.
IPR013313. GPR40_recept_FA.
[Graphical view]
PfamiPF00001. 7tm_1. 1 hit.
[Graphical view]
PRINTSiPR01905. FATTYACIDR.
PR00237. GPCRRHODOPSN.
PR01904. GPR40FAMILY.
PROSITEiPS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8K3T4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDLPPQLSFA LYVSAFALGF PLNLLAIRGA VSHAKLRLTP SLVYTLHLAC
60 70 80 90 100
SDLLLAITLP LKAVEALASG VWPLPLPFCP VFALAHFAPL YAGGGFLAAL
110 120 130 140 150
SAGRYLGAAF PFGYQAIRRP CYSWGVCVAI WALVLCHLGL ALGLEAPRGW
160 170 180 190 200
VDNTTSSLGI NIPVNGSPVC LEAWDPDSAR PARLSFSILL FFLPLVITAF
210 220 230 240 250
CYVGCLRALV HSGLSHKRKL RAAWVAGGAL LTLLLCLGPY NASNVASFIN
260 270 280 290 300
PDLEGSWRKL GLITGAWSVV LNPLVTGYLG TGPGQGTICV TRTPRGTIQK
Length:300
Mass (Da):31,836
Last modified:October 1, 2002 - v1
Checksum:i4E882AB259A5DD4F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF539810 mRNA. Translation: AAN03479.1.
AB095744 mRNA. Translation: BAC82554.1.
RefSeqiNP_695216.1. NM_153304.2.
UniGeneiRn.86438.

Genome annotation databases

GeneIDi266607.
KEGGirno:266607.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF539810 mRNA. Translation: AAN03479.1.
AB095744 mRNA. Translation: BAC82554.1.
RefSeqiNP_695216.1. NM_153304.2.
UniGeneiRn.86438.

3D structure databases

ProteinModelPortaliQ8K3T4.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000028533.

Chemistry

BindingDBiQ8K3T4.
ChEMBLiCHEMBL1795180.

Protein family/group databases

GPCRDBiSearch...

PTM databases

PhosphoSiteiQ8K3T4.

Proteomic databases

PaxDbiQ8K3T4.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi266607.
KEGGirno:266607.

Organism-specific databases

CTDi2864.
RGDi628613. Ffar1.

Phylogenomic databases

eggNOGiENOG410IV8D. Eukaryota.
ENOG4111572. LUCA.
HOGENOMiHOG000236290.
HOVERGENiHBG080696.
InParanoidiQ8K3T4.
KOiK04325.
PhylomeDBiQ8K3T4.

Miscellaneous databases

NextBioi624432.
PROiQ8K3T4.

Family and domain databases

InterProiIPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
IPR013312. GPR40-rel_orph.
IPR013313. GPR40_recept_FA.
[Graphical view]
PfamiPF00001. 7tm_1. 1 hit.
[Graphical view]
PRINTSiPR01905. FATTYACIDR.
PR00237. GPCRRHODOPSN.
PR01904. GPR40FAMILY.
PROSITEiPS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
    Strain: Sprague-Dawley.
  2. Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.

Entry informationi

Entry nameiFFAR1_RAT
AccessioniPrimary (citable) accession number: Q8K3T4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 21, 2006
Last sequence update: October 1, 2002
Last modified: November 11, 2015
This is version 81 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. 7-transmembrane G-linked receptors
    List of 7-transmembrane G-linked receptor entries
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.