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Protein

Free fatty acid receptor 1

Gene

Ffar1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

G-protein coupled receptor for medium and long chain saturated and unsaturated fatty acids that plays an important role in glucose homeostasis. Fatty acid binding increases glucose-stimulated insulin secretion, and may also enhance the secretion of glucagon-like peptide 1 (GLP-1). May also play a role in bone homeostasis; receptor signaling activates pathways that inhibit osteoclast differentiation. Ligand binding leads to a conformation change that triggers signaling via G-proteins that activate phospholipase C, leading to an increase of the intracellular calcium concentration. Seems to act through a G(q) and G(i)-mediated pathway.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei145Important for receptor activationBy similarity1
Sitei172Important for receptor activationBy similarity1
Binding sitei183AgonistBy similarity1
Binding sitei240AgonistBy similarity1
Binding sitei258AgonistBy similarity1

GO - Molecular functioni

  • bioactive lipid receptor activity Source: UniProtKB
  • fatty acid binding Source: RGD

GO - Biological processi

  • glucose homeostasis Source: UniProtKB
  • G-protein coupled receptor signaling pathway Source: RGD
  • insulin secretion Source: RGD
  • positive regulation of calcium ion transport Source: UniProtKB
  • positive regulation of calcium ion transport into cytosol Source: RGD
  • positive regulation of insulin secretion Source: RGD
  • response to fatty acid Source: UniProtKB

Keywordsi

Molecular functionG-protein coupled receptor, Receptor, Transducer
LigandLipid-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Free fatty acid receptor 1
Alternative name(s):
G-protein coupled receptor 40
Gene namesi
Name:Ffar1
Synonyms:Gpr40
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi628613. Ffar1.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 8ExtracellularSequence analysisBy similarity8
Transmembranei9 – 31Helical; Name=1Sequence analysisBy similarityAdd BLAST23
Topological domaini32 – 41CytoplasmicSequence analysisBy similarity10
Transmembranei42 – 64Helical; Name=2Sequence analysisBy similarityAdd BLAST23
Topological domaini65 – 79ExtracellularSequence analysisBy similarityAdd BLAST15
Transmembranei80 – 101Helical; Name=3Sequence analysisBy similarityAdd BLAST22
Topological domaini102 – 121CytoplasmicSequence analysisBy similarityAdd BLAST20
Transmembranei122 – 142Helical; Name=4Sequence analysisBy similarityAdd BLAST21
Topological domaini143 – 178ExtracellularSequence analysisBy similarityAdd BLAST36
Transmembranei179 – 200Helical; Name=5Sequence analysisBy similarityAdd BLAST22
Topological domaini201 – 223CytoplasmicSequence analysisBy similarityAdd BLAST23
Transmembranei224 – 248Helical; Name=6Sequence analysisBy similarityAdd BLAST25
Topological domaini249 – 256ExtracellularSequence analysisBy similarity8
Transmembranei257 – 279Helical; Name=7Sequence analysisBy similarityAdd BLAST23
Topological domaini280 – 300CytoplasmicSequence analysisBy similarityAdd BLAST21

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL1795180.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002277561 – 300Free fatty acid receptor 1Add BLAST300

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi79 ↔ 170By similarity
Glycosylationi153N-linked (GlcNAc...) asparagineSequence analysis1

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiQ8K3T4.

PTM databases

PhosphoSitePlusiQ8K3T4.

Expressioni

Tissue specificityi

Expressed abundantly in pancreatic beta cells.2 Publications

Interactioni

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000028533.

Chemistry databases

BindingDBiQ8K3T4.

Structurei

3D structure databases

ProteinModelPortaliQ8K3T4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni87 – 91Agonist bindingBy similarity5

Sequence similaritiesi

Belongs to the G-protein coupled receptor 1 family.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IV8D. Eukaryota.
ENOG4111572. LUCA.
HOGENOMiHOG000236290.
HOVERGENiHBG080696.
InParanoidiQ8K3T4.
KOiK04325.
PhylomeDBiQ8K3T4.

Family and domain databases

CDDicd15169. 7tmA_FFAR1. 1 hit.
InterProiView protein in InterPro
IPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
IPR013312. GPR40-rel_orph.
IPR013313. GPR40_recept_FA.
PfamiView protein in Pfam
PF00001. 7tm_1. 1 hit.
PRINTSiPR01905. FATTYACIDR.
PR00237. GPCRRHODOPSN.
PR01904. GPR40FAMILY.
PROSITEiView protein in PROSITE
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.

Sequencei

Sequence statusi: Complete.

Q8K3T4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDLPPQLSFA LYVSAFALGF PLNLLAIRGA VSHAKLRLTP SLVYTLHLAC
60 70 80 90 100
SDLLLAITLP LKAVEALASG VWPLPLPFCP VFALAHFAPL YAGGGFLAAL
110 120 130 140 150
SAGRYLGAAF PFGYQAIRRP CYSWGVCVAI WALVLCHLGL ALGLEAPRGW
160 170 180 190 200
VDNTTSSLGI NIPVNGSPVC LEAWDPDSAR PARLSFSILL FFLPLVITAF
210 220 230 240 250
CYVGCLRALV HSGLSHKRKL RAAWVAGGAL LTLLLCLGPY NASNVASFIN
260 270 280 290 300
PDLEGSWRKL GLITGAWSVV LNPLVTGYLG TGPGQGTICV TRTPRGTIQK
Length:300
Mass (Da):31,836
Last modified:October 1, 2002 - v1
Checksum:i4E882AB259A5DD4F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF539810 mRNA. Translation: AAN03479.1.
AB095744 mRNA. Translation: BAC82554.1.
RefSeqiNP_695216.1. NM_153304.2.
UniGeneiRn.86438.

Genome annotation databases

GeneIDi266607.
KEGGirno:266607.

Similar proteinsi

Entry informationi

Entry nameiFFAR1_RAT
AccessioniPrimary (citable) accession number: Q8K3T4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 21, 2006
Last sequence update: October 1, 2002
Last modified: November 22, 2017
This is version 88 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. 7-transmembrane G-linked receptors
    List of 7-transmembrane G-linked receptor entries
  2. SIMILARITY comments
    Index of protein domains and families