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Protein

CCR4-NOT transcription complex subunit 6

Gene

Cnot6

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Poly(A) nuclease with 3'-5' RNase activity. Catalytic component of the CCR4-NOT complex which is one of the major cellular mRNA deadenylases and is linked to various cellular processes including bulk mRNA degradation, miRNA-mediated repression, translational repression during translational initiation and general transcription regulation. Additional complex functions may be a consequence of its influence on mRNA expression. Involved in mRNA decay mediated by the major-protein-coding determinant of instability (mCRD) of the FOS gene in the cytoplasm. In the presence of ZNF335, enhances ligand-dependent transcriptional activity of nuclear hormone receptors. Mediates cell proliferation and cell survival and prevents cellular senescence (By similarity).By similarity

Catalytic activityi

Exonucleolytic cleavage of poly(A) to 5'-AMP.

Cofactori

Mg2+By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei240 – 2401By similarity
Metal bindingi240 – 2401MagnesiumBy similarity
Metal bindingi240 – 2401Magnesium 1By similarity
Metal bindingi412 – 4121Magnesium 2By similarity
Metal bindingi414 – 4141Magnesium 2By similarity
Active sitei491 – 4911By similarity
Active sitei531 – 5311By similarity
Metal bindingi531 – 5311Magnesium 2By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Activator, Exonuclease, Hydrolase, Nuclease

Keywords - Biological processi

RNA-mediated gene silencing, Transcription, Transcription regulation, Translation regulation

Keywords - Ligandi

Magnesium, Metal-binding, RNA-binding

Enzyme and pathway databases

ReactomeiR-MMU-6804115. TP53 regulates transcription of additional cell cycle genes whose exact role in the p53 pathway remain uncertain.

Names & Taxonomyi

Protein namesi
Recommended name:
CCR4-NOT transcription complex subunit 6 (EC:3.1.13.4)
Alternative name(s):
CCR4 carbon catabolite repression 4-like
Carbon catabolite repressor protein 4 homolog
Cytoplasmic deadenylase
Gene namesi
Name:Cnot6
Synonyms:Ccr4, Kiaa1194
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 11

Organism-specific databases

MGIiMGI:2144529. Cnot6.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 557557CCR4-NOT transcription complex subunit 6PRO_0000218574Add
BLAST

Proteomic databases

MaxQBiQ8K3P5.
PaxDbiQ8K3P5.
PeptideAtlasiQ8K3P5.
PRIDEiQ8K3P5.

PTM databases

iPTMnetiQ8K3P5.
PhosphoSiteiQ8K3P5.

Expressioni

Developmental stagei

Expressed in embryonic stem (ES) cells.1 Publication

Gene expression databases

BgeeiENSMUSG00000020362.
CleanExiMM_CCR4.
MM_CNOT6.
GenevisibleiQ8K3P5. MM.

Interactioni

Subunit structurei

Component of the CCR4-NOT complex; distinct complexes seem to exist that differ in the participation of probably mutually exclusive catalytic subunits; the complex contains two deadenylase subunits, CNOT6 or CNOT6L, and CNOT7 or CNOT8 (By simialrity). Interacts with UNR and ZNF335.1 Publication

Protein-protein interaction databases

IntActiQ8K3P5. 1 interaction.
STRINGi10090.ENSMUSP00000020624.

Structurei

3D structure databases

ProteinModelPortaliQ8K3P5.
SMRiQ8K3P5. Positions 28-540.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati52 – 7322LRR 1Add
BLAST
Repeati75 – 9622LRR 2Add
BLAST
Repeati98 – 12023LRR 3Add
BLAST
Repeati121 – 14323LRR 4Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni153 – 557405Nuclease domainBy similarityAdd
BLAST

Sequence similaritiesi

Belongs to the CCR4/nocturin family.Curated
Contains 4 LRR (leucine-rich) repeats.Curated

Keywords - Domaini

Leucine-rich repeat, Repeat

Phylogenomic databases

eggNOGiKOG0620. Eukaryota.
COG4886. LUCA.
COG5239. LUCA.
GeneTreeiENSGT00550000074364.
HOGENOMiHOG000294222.
HOVERGENiHBG052641.
InParanoidiQ8K3P5.
KOiK12603.
OMAiHLGMEKQ.
OrthoDBiEOG091G05MA.
PhylomeDBiQ8K3P5.
TreeFamiTF323175.

Family and domain databases

Gene3Di3.60.10.10. 2 hits.
3.80.10.10. 1 hit.
InterProiIPR005135. Endo/exonuclease/phosphatase.
IPR032675. L_dom-like.
IPR001611. Leu-rich_rpt.
IPR003591. Leu-rich_rpt_typical-subtyp.
[Graphical view]
PfamiPF03372. Exo_endo_phos. 1 hit.
PF13855. LRR_8. 1 hit.
[Graphical view]
SMARTiSM00369. LRR_TYP. 3 hits.
[Graphical view]
SUPFAMiSSF52058. SSF52058. 1 hit.
SSF56219. SSF56219. 2 hits.
PROSITEiPS51450. LRR. 4 hits.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8K3P5-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MPKEKYEPPD PRRMYTIMSS EEAANGKKSH WAELEISGKV RSLSSSLWSL
60 70 80 90 100
THLTALHLSD NSLSCIPSDI AKLHNLVYLD LSHNQIQSLP AELGNMVSLR
110 120 130 140 150
ELHLNYNQLR VLPFELGKLF QLQTLSLKGN PLTQDILNLC LEPDGTRRLL
160 170 180 190 200
NYLLDNLSGT AKRISTEQPP PRSWIMLQEP DRTRPTALFS VMCYNVLCDK
210 220 230 240 250
YATRQLYGYC PSWALNWDYR KKAIIQEILS CNADIISLQE VETEQYYSFF
260 270 280 290 300
LVELKERGYN GFFSPKSRAR TMSEQERKHV DGCAIFFKTE KFTLVQKHTV
310 320 330 340 350
EFNQLAMANS EGSEAMLNRV MTKDNIGVAV LLELRKELIE MSSGKPHLGT
360 370 380 390 400
EKQLILVANA HMHWDPEYSD VKLVQTMMFL SEVKNIIDKA SRSLKSSVLG
410 420 430 440 450
ECGTIPLVLC ADLNSLPDSG VVEYLSTGGV ETNHKDFKEL RYNESLTNFS
460 470 480 490 500
CNGKNGMTNG RITHGFKLKS AYENGLMPYT NYTFDFKGII DYIFYSKPQL
510 520 530 540 550
NTLAILGPLD HHWLVENNIS GCPHPLIPSD HFSLFAQLEL LLPFLPQVNG

IHLPGRR
Length:557
Mass (Da):63,304
Last modified:April 13, 2004 - v2
Checksum:iE2780FC2D56EF3F7
GO
Isoform 2 (identifier: Q8K3P5-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     159-164: GTAKRI → V

Note: No experimental confirmation available.
Show »
Length:552
Mass (Da):62,776
Checksum:i5DC598040C453EFD
GO
Isoform 3 (identifier: Q8K3P5-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-191: Missing.

Note: No experimental confirmation available.
Show »
Length:366
Mass (Da):41,652
Checksum:i9FEB6AD872C2E059
GO

Sequence cautioni

The sequence BAC98117 differs from that shown. Reason: Erroneous initiation. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti198 – 1981C → G in AAK85707 (PubMed:11747467).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 191191Missing in isoform 3. 1 PublicationVSP_009938Add
BLAST
Alternative sequencei159 – 1646GTAKRI → V in isoform 2. 1 PublicationVSP_009939

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY043269 mRNA. Translation: AAK85707.1.
AK129307 mRNA. Translation: BAC98117.1. Different initiation.
AL606479 Genomic DNA. Translation: CAI23937.1.
BC049984 mRNA. Translation: AAH49984.1.
BC057190 mRNA. Translation: AAH57190.1.
BC062950 mRNA. Translation: AAH62950.1.
CCDSiCCDS24621.1. [Q8K3P5-2]
CCDS70172.1. [Q8K3P5-1]
RefSeqiNP_001277670.1. NM_001290741.1. [Q8K3P5-1]
NP_997649.1. NM_212484.2. [Q8K3P5-2]
XP_006532003.1. XM_006531940.2. [Q8K3P5-1]
XP_006532004.1. XM_006531941.2. [Q8K3P5-1]
XP_006532005.1. XM_006531942.1. [Q8K3P5-1]
XP_011246967.1. XM_011248665.1. [Q8K3P5-1]
UniGeneiMm.247113.
Mm.431753.

Genome annotation databases

EnsembliENSMUST00000020624; ENSMUSP00000020624; ENSMUSG00000020362. [Q8K3P5-2]
ENSMUST00000145353; ENSMUSP00000121239; ENSMUSG00000020362. [Q8K3P5-1]
GeneIDi104625.
KEGGimmu:104625.
UCSCiuc007iqx.2. mouse. [Q8K3P5-1]
uc007irb.3. mouse. [Q8K3P5-2]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY043269 mRNA. Translation: AAK85707.1.
AK129307 mRNA. Translation: BAC98117.1. Different initiation.
AL606479 Genomic DNA. Translation: CAI23937.1.
BC049984 mRNA. Translation: AAH49984.1.
BC057190 mRNA. Translation: AAH57190.1.
BC062950 mRNA. Translation: AAH62950.1.
CCDSiCCDS24621.1. [Q8K3P5-2]
CCDS70172.1. [Q8K3P5-1]
RefSeqiNP_001277670.1. NM_001290741.1. [Q8K3P5-1]
NP_997649.1. NM_212484.2. [Q8K3P5-2]
XP_006532003.1. XM_006531940.2. [Q8K3P5-1]
XP_006532004.1. XM_006531941.2. [Q8K3P5-1]
XP_006532005.1. XM_006531942.1. [Q8K3P5-1]
XP_011246967.1. XM_011248665.1. [Q8K3P5-1]
UniGeneiMm.247113.
Mm.431753.

3D structure databases

ProteinModelPortaliQ8K3P5.
SMRiQ8K3P5. Positions 28-540.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ8K3P5. 1 interaction.
STRINGi10090.ENSMUSP00000020624.

PTM databases

iPTMnetiQ8K3P5.
PhosphoSiteiQ8K3P5.

Proteomic databases

MaxQBiQ8K3P5.
PaxDbiQ8K3P5.
PeptideAtlasiQ8K3P5.
PRIDEiQ8K3P5.

Protocols and materials databases

DNASUi104625.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000020624; ENSMUSP00000020624; ENSMUSG00000020362. [Q8K3P5-2]
ENSMUST00000145353; ENSMUSP00000121239; ENSMUSG00000020362. [Q8K3P5-1]
GeneIDi104625.
KEGGimmu:104625.
UCSCiuc007iqx.2. mouse. [Q8K3P5-1]
uc007irb.3. mouse. [Q8K3P5-2]

Organism-specific databases

CTDi57472.
MGIiMGI:2144529. Cnot6.
RougeiSearch...

Phylogenomic databases

eggNOGiKOG0620. Eukaryota.
COG4886. LUCA.
COG5239. LUCA.
GeneTreeiENSGT00550000074364.
HOGENOMiHOG000294222.
HOVERGENiHBG052641.
InParanoidiQ8K3P5.
KOiK12603.
OMAiHLGMEKQ.
OrthoDBiEOG091G05MA.
PhylomeDBiQ8K3P5.
TreeFamiTF323175.

Enzyme and pathway databases

ReactomeiR-MMU-6804115. TP53 regulates transcription of additional cell cycle genes whose exact role in the p53 pathway remain uncertain.

Miscellaneous databases

PROiQ8K3P5.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000020362.
CleanExiMM_CCR4.
MM_CNOT6.
GenevisibleiQ8K3P5. MM.

Family and domain databases

Gene3Di3.60.10.10. 2 hits.
3.80.10.10. 1 hit.
InterProiIPR005135. Endo/exonuclease/phosphatase.
IPR032675. L_dom-like.
IPR001611. Leu-rich_rpt.
IPR003591. Leu-rich_rpt_typical-subtyp.
[Graphical view]
PfamiPF03372. Exo_endo_phos. 1 hit.
PF13855. LRR_8. 1 hit.
[Graphical view]
SMARTiSM00369. LRR_TYP. 3 hits.
[Graphical view]
SUPFAMiSSF52058. SSF52058. 1 hit.
SSF56219. SSF56219. 2 hits.
PROSITEiPS51450. LRR. 4 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCNOT6_MOUSE
AccessioniPrimary (citable) accession number: Q8K3P5
Secondary accession number(s): Q5NCL3, Q80V15
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 13, 2004
Last sequence update: April 13, 2004
Last modified: September 7, 2016
This is version 128 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.