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Protein

CCR4-NOT transcription complex subunit 6

Gene

Cnot6

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Poly(A) nuclease with 3'-5' RNase activity. Catalytic component of the CCR4-NOT complex which is one of the major cellular mRNA deadenylases and is linked to various cellular processes including bulk mRNA degradation, miRNA-mediated repression, translational repression during translational initiation and general transcription regulation. Additional complex functions may be a consequence of its influence on mRNA expression. Involved in mRNA decay mediated by the major-protein-coding determinant of instability (mCRD) of the FOS gene in the cytoplasm. In the presence of ZNF335, enhances ligand-dependent transcriptional activity of nuclear hormone receptors. Mediates cell proliferation and cell survival and prevents cellular senescence (By similarity).By similarity

Catalytic activityi

Exonucleolytic cleavage of poly(A) to 5'-AMP.

Cofactori

Mg2+By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei240By similarity1
Metal bindingi240MagnesiumBy similarity1
Metal bindingi240Magnesium 1By similarity1
Metal bindingi412Magnesium 2By similarity1
Metal bindingi414Magnesium 2By similarity1
Active sitei491By similarity1
Active sitei531By similarity1
Metal bindingi531Magnesium 2By similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionActivator, Exonuclease, Hydrolase, Nuclease, RNA-binding
Biological processRNA-mediated gene silencing, Transcription, Transcription regulation, Translation regulation
LigandMagnesium, Metal-binding

Enzyme and pathway databases

ReactomeiR-MMU-6804115 TP53 regulates transcription of additional cell cycle genes whose exact role in the p53 pathway remain uncertain

Names & Taxonomyi

Protein namesi
Recommended name:
CCR4-NOT transcription complex subunit 6 (EC:3.1.13.4)
Alternative name(s):
CCR4 carbon catabolite repression 4-like
Carbon catabolite repressor protein 4 homolog
Cytoplasmic deadenylase
Gene namesi
Name:Cnot6
Synonyms:Ccr4, Kiaa1194
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 11

Organism-specific databases

MGIiMGI:2144529 Cnot6

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002185741 – 557CCR4-NOT transcription complex subunit 6Add BLAST557

Proteomic databases

PaxDbiQ8K3P5
PeptideAtlasiQ8K3P5
PRIDEiQ8K3P5

PTM databases

iPTMnetiQ8K3P5
PhosphoSitePlusiQ8K3P5

Expressioni

Developmental stagei

Expressed in embryonic stem (ES) cells.1 Publication

Gene expression databases

BgeeiENSMUSG00000020362
CleanExiMM_CCR4
MM_CNOT6
GenevisibleiQ8K3P5 MM

Interactioni

Subunit structurei

Component of the CCR4-NOT complex; distinct complexes seem to exist that differ in the participation of probably mutually exclusive catalytic subunits; the complex contains two deadenylase subunits, CNOT6 or CNOT6L, and CNOT7 or CNOT8. Interacts with CNOT7 and CNOT8 (By similarity). Interacts with UNR (PubMed:15314026). Interacts with ZFP36L1 (via N-terminus). Interacts with ZNF335 (By similarity).By similarity1 Publication

Protein-protein interaction databases

IntActiQ8K3P5, 1 interactor
STRINGi10090.ENSMUSP00000020624

Structurei

3D structure databases

ProteinModelPortaliQ8K3P5
SMRiQ8K3P5
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati52 – 73LRR 1Add BLAST22
Repeati75 – 96LRR 2Add BLAST22
Repeati98 – 120LRR 3Add BLAST23
Repeati121 – 143LRR 4Add BLAST23

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni153 – 557Nuclease domainBy similarityAdd BLAST405

Sequence similaritiesi

Belongs to the CCR4/nocturin family.Curated

Keywords - Domaini

Leucine-rich repeat, Repeat

Phylogenomic databases

eggNOGiKOG0620 Eukaryota
COG4886 LUCA
COG5239 LUCA
GeneTreeiENSGT00550000074364
HOGENOMiHOG000294222
HOVERGENiHBG052641
InParanoidiQ8K3P5
KOiK12603
OMAiWPKSRYK
OrthoDBiEOG091G05MA
PhylomeDBiQ8K3P5
TreeFamiTF323175

Family and domain databases

CDDicd10313 Deadenylase_CCR4a, 1 hit
Gene3Di3.60.10.10, 1 hit
3.80.10.10, 1 hit
InterProiView protein in InterPro
IPR034966 Cnot6
IPR036691 Endo/exonu/phosph_ase_sf
IPR005135 Endo/exonuclease/phosphatase
IPR001611 Leu-rich_rpt
IPR003591 Leu-rich_rpt_typical-subtyp
IPR032675 LRR_dom_sf
PfamiView protein in Pfam
PF03372 Exo_endo_phos, 1 hit
PF13855 LRR_8, 1 hit
SMARTiView protein in SMART
SM00369 LRR_TYP, 3 hits
SUPFAMiSSF56219 SSF56219, 2 hits
PROSITEiView protein in PROSITE
PS51450 LRR, 4 hits

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8K3P5-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MPKEKYEPPD PRRMYTIMSS EEAANGKKSH WAELEISGKV RSLSSSLWSL
60 70 80 90 100
THLTALHLSD NSLSCIPSDI AKLHNLVYLD LSHNQIQSLP AELGNMVSLR
110 120 130 140 150
ELHLNYNQLR VLPFELGKLF QLQTLSLKGN PLTQDILNLC LEPDGTRRLL
160 170 180 190 200
NYLLDNLSGT AKRISTEQPP PRSWIMLQEP DRTRPTALFS VMCYNVLCDK
210 220 230 240 250
YATRQLYGYC PSWALNWDYR KKAIIQEILS CNADIISLQE VETEQYYSFF
260 270 280 290 300
LVELKERGYN GFFSPKSRAR TMSEQERKHV DGCAIFFKTE KFTLVQKHTV
310 320 330 340 350
EFNQLAMANS EGSEAMLNRV MTKDNIGVAV LLELRKELIE MSSGKPHLGT
360 370 380 390 400
EKQLILVANA HMHWDPEYSD VKLVQTMMFL SEVKNIIDKA SRSLKSSVLG
410 420 430 440 450
ECGTIPLVLC ADLNSLPDSG VVEYLSTGGV ETNHKDFKEL RYNESLTNFS
460 470 480 490 500
CNGKNGMTNG RITHGFKLKS AYENGLMPYT NYTFDFKGII DYIFYSKPQL
510 520 530 540 550
NTLAILGPLD HHWLVENNIS GCPHPLIPSD HFSLFAQLEL LLPFLPQVNG

IHLPGRR
Length:557
Mass (Da):63,304
Last modified:April 13, 2004 - v2
Checksum:iE2780FC2D56EF3F7
GO
Isoform 2 (identifier: Q8K3P5-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     159-164: GTAKRI → V

Note: No experimental confirmation available.
Show »
Length:552
Mass (Da):62,776
Checksum:i5DC598040C453EFD
GO
Isoform 3 (identifier: Q8K3P5-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-191: Missing.

Note: No experimental confirmation available.
Show »
Length:366
Mass (Da):41,652
Checksum:i9FEB6AD872C2E059
GO

Sequence cautioni

The sequence BAC98117 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti198C → G in AAK85707 (PubMed:11747467).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0099381 – 191Missing in isoform 3. 1 PublicationAdd BLAST191
Alternative sequenceiVSP_009939159 – 164GTAKRI → V in isoform 2. 1 Publication6

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY043269 mRNA Translation: AAK85707.1
AK129307 mRNA Translation: BAC98117.1 Different initiation.
AL606479 Genomic DNA No translation available.
BC049984 mRNA Translation: AAH49984.1
BC057190 mRNA Translation: AAH57190.1
BC062950 mRNA Translation: AAH62950.1
CCDSiCCDS24621.1 [Q8K3P5-2]
CCDS70172.1 [Q8K3P5-1]
RefSeqiNP_001277670.1, NM_001290741.1 [Q8K3P5-1]
NP_997649.1, NM_212484.2 [Q8K3P5-2]
XP_006532003.1, XM_006531940.2 [Q8K3P5-1]
XP_006532004.1, XM_006531941.3 [Q8K3P5-1]
XP_006532005.1, XM_006531942.1 [Q8K3P5-1]
XP_011246967.1, XM_011248665.2 [Q8K3P5-1]
UniGeneiMm.247113
Mm.431753

Genome annotation databases

EnsembliENSMUST00000020624; ENSMUSP00000020624; ENSMUSG00000020362 [Q8K3P5-2]
ENSMUST00000145353; ENSMUSP00000121239; ENSMUSG00000020362 [Q8K3P5-1]
GeneIDi104625
KEGGimmu:104625
UCSCiuc007iqx.2 mouse [Q8K3P5-1]
uc007irb.3 mouse [Q8K3P5-2]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiCNOT6_MOUSE
AccessioniPrimary (citable) accession number: Q8K3P5
Secondary accession number(s): Q5NCL3, Q80V15
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 13, 2004
Last sequence update: April 13, 2004
Last modified: May 23, 2018
This is version 141 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

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