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Protein

Optineurin

Gene

Optn

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Plays an important role in the maintenance of the Golgi complex, in membrane trafficking, in exocytosis, through its interaction with myosin VI and Rab8. Links myosin VI to the Golgi complex and plays an important role in Golgi ribbon formation. Negatively regulates the induction of IFNB in response to RNA virus infection. Plays a neuroprotective role in the eye and optic nerve. Probably part of the TNF-alpha signaling pathway that can shift the equilibrium toward induction of cell death. May act by regulating membrane trafficking and cellular morphogenesis via a complex that contains Rab8 and hungtingtin (HD). Mediates the interaction of Rab8 with the probable GTPase-activating protein TBC1D17 during Rab8-mediated endocytic trafficking, such as of transferrin receptor (TFRC/TfR); regulates Rab8 recruitnment to tubules emanating from the endocytic recycling compartment. Autophagy receptor that interacts directly with both the cargo to become degraded and an autophagy modifier of the MAP1 LC3 family; targets ubiquitin-coated bacteria (xenophagy) and appears to function in the same pathway as SQSTM1 and CALCOCO2/NDP52 (By similarity).By similarity

GO - Molecular functioni

  1. K63-linked polyubiquitin binding Source: MGI
  2. polyubiquitin binding Source: MGI
  3. protein C-terminus binding Source: MGI
  4. Rab GTPase binding Source: MGI

GO - Biological processi

  1. cellular protein localization Source: MGI
  2. defense response to Gram-negative bacterium Source: MGI
  3. Golgi organization Source: MGI
  4. Golgi ribbon formation Source: UniProtKB
  5. Golgi to plasma membrane protein transport Source: MGI
  6. macroautophagy Source: MGI
  7. negative regulation of I-kappaB kinase/NF-kappaB signaling Source: MGI
  8. negative regulation of receptor recycling Source: MGI
  9. protein targeting to Golgi Source: MGI
Complete GO annotation...

Keywords - Biological processi

Autophagy

Enzyme and pathway databases

ReactomeiREACT_345278. Regulation of PLK1 Activity at G2/M Transition.

Names & Taxonomyi

Protein namesi
Recommended name:
Optineurin
Gene namesi
Name:Optn
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 2

Organism-specific databases

MGIiMGI:1918898. Optn.

Subcellular locationi

  1. Cytoplasmperinuclear region By similarity
  2. Golgi apparatus By similarity
  3. Golgi apparatustrans-Golgi network By similarity
  4. Cytoplasmic vesicleautophagosome By similarity
  5. Cytoplasmic vesicle By similarity
  6. Recycling endosome By similarity

  7. Note: Found in the perinuclear region and associates with the Golgi apparatus. Colocalizes with MYO6 and RAB8 at the Golgi complex and in vesicular structures close to the plasma membrane. Localizes to LC3-positive cytoplasmic vesicles upon induction of autophagy (By similarity).By similarity

GO - Cellular componenti

  1. autophagic vacuole Source: UniProtKB-SubCell
  2. cytoplasm Source: MGI
  3. cytoplasmic membrane-bounded vesicle Source: GO_Central
  4. Golgi apparatus Source: MGI
  5. nucleus Source: GO_Central
  6. perinuclear region of cytoplasm Source: MGI
  7. recycling endosome Source: UniProtKB-SubCell
  8. trans-Golgi network Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoplasmic vesicle, Endosome, Golgi apparatus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi50 – 501E → K: Disrupts interaction with Rab8; accumulation in the retinal outer plexiform layer; loss of retinal ganglion cells and connecting synapses, degeneration of entire retina without elevation of intraocular presssure. 2 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 584584OptineurinPRO_0000058069Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei187 – 1871Phosphoserine; by TBK1By similarity
Modified residuei345 – 3451PhosphoserineBy similarity

Post-translational modificationi

Phosphorylated by TBK1, leading to restrict bacterial proliferation in case of infection.By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ8K3K8.
PaxDbiQ8K3K8.
PRIDEiQ8K3K8.

PTM databases

PhosphoSiteiQ8K3K8.

Expressioni

Tissue specificityi

In eye, it is expressed in anterior segment, retina, and optic nerve blood vessels (at protein level). Highly expressed in adult liver, heart and testis.1 Publication

Developmental stagei

Expressed from 7-day-old embryos.1 Publication

Gene expression databases

BgeeiQ8K3K8.
CleanExiMM_OPTN.
GenevestigatoriQ8K3K8.

Interactioni

Subunit structurei

Interacts with HD, Rab8 (RAB8A and/or RAB8B) (active GTP-bound form), GTF3A, TRAF3, TBK1, MYO6 and TFRC. Binds to linear ubiquitin chains. Interacts with LC3 family members MAP1LC3A, MAP1LC3B, GABARAP, GABARAPL1 and GABARAPL2; OPTN phosphorylation increases the asscociation (at least with MAP1LC3B). Self-associates. Interacts with RAB12; the interaction may be indirect.1 Publication

Protein-protein interaction databases

BioGridi214832. 6 interactions.
IntActiQ8K3K8. 2 interactions.

Structurei

3D structure databases

ProteinModelPortaliQ8K3K8.
SMRiQ8K3K8. Positions 424-510, 557-584.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni58 – 219162Interaction with Rab8By similarityAdd
BLAST
Regioni414 – 584171Interaction with HDBy similarityAdd
BLAST
Regioni415 – 524110Interaction with MYO6By similarityAdd
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili38 – 180143Sequence AnalysisAdd
BLAST
Coiled coili243 – 27836Sequence AnalysisAdd
BLAST
Coiled coili307 – 511205Sequence AnalysisAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi186 – 1916LIR
Motifi477 – 4826UBAN

Domaini

Ubiquitin-binding motif (UBAN) is essential for its inhibitory function, subcellular localization and interaction with TBK1.By similarity
The LIR (LC3-interacting region) motif mediates the interaction with ATG8 family proteins.By similarity

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiNOG138369.
GeneTreeiENSGT00530000063808.
HOGENOMiHOG000231899.
HOVERGENiHBG106481.
InParanoidiQ8K3K8.
OMAiAHPNLDT.
OrthoDBiEOG7D2FD7.
PhylomeDBiQ8K3K8.
TreeFamiTF326608.

Family and domain databases

InterProiIPR021063. NEMO_N.
[Graphical view]
PfamiPF11577. NEMO. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8K3K8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSHQPLSCLT EKGDSPCETP GNGPSNMVHP SLDTFTPEEL LQQMKELLVE
60 70 80 90 100
NHQLKEAMKL NNQAMKGRFE ELSAWTEKQK EERLLFEMQS KEVKERLKAL
110 120 130 140 150
THENERLKEE LGKFKEKSEK PLEDLTGGYR YPRALEEEVE KLKTQVEQEV
160 170 180 190 200
EHLKIQVMRL RAEKADLLGI VSELQLKLNS GGSSEDSFVE IRMTEGETEG
210 220 230 240 250
AMKEMKNCPT PTRTDPISLS NCTEDARSCA EFEELTVSQL LLCLREGNQK
260 270 280 290 300
VERLEVALRE AKERISDFEK KANGHSSTEK QTARRADREK EDKGQESVGS
310 320 330 340 350
EVETLSIQVT SLFKELQEAH TKLSEAELMK KRLQEKCQAL ERKNSATPSE
360 370 380 390 400
LNEKQELVYS NKKLELQVES MRSEIKMEQA KTEEEKSRLA TLQATHNKLL
410 420 430 440 450
QEHNKALKTI EELTKQQAEK VDKMLLQELS EKLELAEQAL ASKQLQMDEM
460 470 480 490 500
KQTLAKQEED LETMAVLRAQ MEVYCSDFHA ERAAREKIHE EKEQLALQLA
510 520 530 540 550
ILLKENNDIE EGGSRQSLME MQCRHGARTS DSDQQTYLFQ RGAEDRSWQH
560 570 580
GQQPRSIPIH SCPKCGEVLP DIDTLQIHVM DCII
Length:584
Mass (Da):67,018
Last modified:October 1, 2002 - v1
Checksum:i4BCF3BD4F71921F0
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY071834 mRNA. Translation: AAL61853.1.
AY340635
, AY340623, AY340624, AY340625, AY340626, AY340627, AY340628, AY340629, AY340630, AY340631, AY340632, AY340633, AY340634 Genomic DNA. Translation: AAQ21118.1.
AL928662 Genomic DNA. Translation: CAM22578.1.
BC061185 mRNA. Translation: AAH61185.1.
CCDSiCCDS15663.1.
RefSeqiNP_862896.1. NM_181848.4.
XP_006497609.1. XM_006497546.1.
XP_006497610.1. XM_006497547.2.
UniGeneiMm.116942.

Genome annotation databases

EnsembliENSMUST00000027986; ENSMUSP00000027986; ENSMUSG00000026672.
ENSMUST00000114996; ENSMUSP00000110648; ENSMUSG00000026672.
GeneIDi71648.
KEGGimmu:71648.
UCSCiuc008ifm.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY071834 mRNA. Translation: AAL61853.1.
AY340635
, AY340623, AY340624, AY340625, AY340626, AY340627, AY340628, AY340629, AY340630, AY340631, AY340632, AY340633, AY340634 Genomic DNA. Translation: AAQ21118.1.
AL928662 Genomic DNA. Translation: CAM22578.1.
BC061185 mRNA. Translation: AAH61185.1.
CCDSiCCDS15663.1.
RefSeqiNP_862896.1. NM_181848.4.
XP_006497609.1. XM_006497546.1.
XP_006497610.1. XM_006497547.2.
UniGeneiMm.116942.

3D structure databases

ProteinModelPortaliQ8K3K8.
SMRiQ8K3K8. Positions 424-510, 557-584.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi214832. 6 interactions.
IntActiQ8K3K8. 2 interactions.

PTM databases

PhosphoSiteiQ8K3K8.

Proteomic databases

MaxQBiQ8K3K8.
PaxDbiQ8K3K8.
PRIDEiQ8K3K8.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000027986; ENSMUSP00000027986; ENSMUSG00000026672.
ENSMUST00000114996; ENSMUSP00000110648; ENSMUSG00000026672.
GeneIDi71648.
KEGGimmu:71648.
UCSCiuc008ifm.1. mouse.

Organism-specific databases

CTDi10133.
MGIiMGI:1918898. Optn.

Phylogenomic databases

eggNOGiNOG138369.
GeneTreeiENSGT00530000063808.
HOGENOMiHOG000231899.
HOVERGENiHBG106481.
InParanoidiQ8K3K8.
OMAiAHPNLDT.
OrthoDBiEOG7D2FD7.
PhylomeDBiQ8K3K8.
TreeFamiTF326608.

Enzyme and pathway databases

ReactomeiREACT_345278. Regulation of PLK1 Activity at G2/M Transition.

Miscellaneous databases

NextBioi334153.
PROiQ8K3K8.
SOURCEiSearch...

Gene expression databases

BgeeiQ8K3K8.
CleanExiMM_OPTN.
GenevestigatoriQ8K3K8.

Family and domain databases

InterProiIPR021063. NEMO_N.
[Graphical view]
PfamiPF11577. NEMO. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Molecular cloning, genomic structure, and protein characterization of mouse optineurin."
    Rezaie T., Sarfarazi M.
    Genomics 85:131-138(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], SUBCELLULAR LOCATION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
    Strain: 129.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Kidney.
  4. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Liver.
  5. "Overexpression of optineurin E50K disrupts Rab8 interaction and leads to a progressive retinal degeneration in mice."
    Chi Z.L., Akahori M., Obazawa M., Minami M., Noda T., Nakaya N., Tomarev S., Kawase K., Yamamoto T., Noda S., Sasaoka M., Shimazaki A., Takada Y., Iwata T.
    Hum. Mol. Genet. 19:2606-2615(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH RAB8, MUTAGENESIS OF GLU-50.
  6. "Enhanced optineurin E50K-TBK1 interaction evokes protein insolubility and initiates familial primary open-angle glaucoma."
    Minegishi Y., Iejima D., Kobayashi H., Chi Z.L., Kawase K., Yamamoto T., Seki T., Yuasa S., Fukuda K., Iwata T.
    Hum. Mol. Genet. 22:3559-3567(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: MUTAGENESIS OF GLU-50.

Entry informationi

Entry nameiOPTN_MOUSE
AccessioniPrimary (citable) accession number: Q8K3K8
Secondary accession number(s): A2ASP3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 29, 2005
Last sequence update: October 1, 2002
Last modified: April 1, 2015
This is version 88 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

OPTN E59K transgenic mice exhibit profound gliosis in the retina.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.