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Protein

Optineurin

Gene

Optn

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Plays an important role in the maintenance of the Golgi complex, in membrane trafficking, in exocytosis, through its interaction with myosin VI and Rab8. Links myosin VI to the Golgi complex and plays an important role in Golgi ribbon formation. Plays a role in the activation of innate immune response during viral infection. Mechanistically, recruits TBK1 at the Golgi apparatus, promoting its trans-phosphorylation after RLR or TLR3 stimulation. In turn, activated TBK1 phosphorylates its downstream partner IRF3 to produce IFN-beta. Plays a neuroprotective role in the eye and optic nerve. May act by regulating membrane trafficking and cellular morphogenesis via a complex that contains Rab8 and hungtingtin (HD). Mediates the interaction of Rab8 with the probable GTPase-activating protein TBC1D17 during Rab8-mediated endocytic trafficking, such as of transferrin receptor (TFRC/TfR); regulates Rab8 recruitnment to tubules emanating from the endocytic recycling compartment. Autophagy receptor that interacts directly with both the cargo to become degraded and an autophagy modifier of the MAP1 LC3 family; targets ubiquitin-coated bacteria (xenophagy), such as cytoplasmic Salmonella enterica, and appears to function in the same pathway as SQSTM1 and CALCOCO2/NDP52. May constitute a cellular target for adenovirus E3 14.7, an inhibitor of TNF-alpha functions, thereby affecting cell death.By similarity1 Publication

Miscellaneous

OPTN E59K transgenic mice exhibit profound gliosis in the retina.
OPTN-deficient cells display reduced TBK1 activation, IRF3 phosphorylation, and expression of type I IFNs in response to TLR3 and TLR4 stimulation, indicating a role for OPTN in the innate immune response.1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri554 – 584CCHC NOA-typePROSITE-ProRule annotationAdd BLAST31

GO - Molecular functioni

GO - Biological processi

Keywordsi

Biological processAutophagy, Immunity, Innate immunity
LigandMetal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-MMU-2565942. Regulation of PLK1 Activity at G2/M Transition.
R-MMU-8854214. TBC/RABGAPs.

Names & Taxonomyi

Protein namesi
Recommended name:
Optineurin
Gene namesi
Name:Optn
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 2

Organism-specific databases

MGIiMGI:1918898. Optn.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Cytoplasmic vesicle, Endosome, Golgi apparatus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi50E → K: Disrupts interaction with Rab8; accumulation in the retinal outer plexiform layer; loss of retinal ganglion cells and connecting synapses, degeneration of entire retina without elevation of intraocular pressure. 2 Publications1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000580691 – 584OptineurinAdd BLAST584

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei187PhosphoserineCombined sources1
Modified residuei345PhosphoserineCombined sources1
Modified residuei530PhosphoserineBy similarity1

Post-translational modificationi

Phosphorylated by TBK1, leading to restrict bacterial proliferation in case of infection.By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ8K3K8.
MaxQBiQ8K3K8.
PaxDbiQ8K3K8.
PeptideAtlasiQ8K3K8.
PRIDEiQ8K3K8.

PTM databases

iPTMnetiQ8K3K8.
PhosphoSitePlusiQ8K3K8.

Expressioni

Tissue specificityi

In eye, it is expressed in anterior segment, retina, and optic nerve blood vessels (at protein level). Highly expressed in adult liver, heart and testis.1 Publication

Developmental stagei

Expressed from 7-day-old embryos.1 Publication

Gene expression databases

BgeeiENSMUSG00000026672.
CleanExiMM_OPTN.
GenevisibleiQ8K3K8. MM.

Interactioni

Subunit structurei

Interacts with HD, Rab8 (RAB8A and/or RAB8B) (active GTP-bound form), GTF3A, TRAF3, TBK1, MYO6 and TFRC. Binds to linear ubiquitin chains. Interacts with LC3 family members MAP1LC3A, MAP1LC3B, GABARAP, GABARAPL1 and GABARAPL2; OPTN phosphorylation increases the association (at least with MAP1LC3B). Self-associates. Interacts with RAB12; the interaction may be indirect. Interacts with TBK1; this interaction leads to the Golgi localization of TBK1 and its subsequent activation.By similarity1 Publication

GO - Molecular functioni

Protein-protein interaction databases

BioGridi214832. 8 interactors.
IntActiQ8K3K8. 3 interactors.
STRINGi10090.ENSMUSP00000027986.

Structurei

3D structure databases

ProteinModelPortaliQ8K3K8.
SMRiQ8K3K8.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni58 – 219Interaction with Rab8By similarityAdd BLAST162
Regioni414 – 584Interaction with HDBy similarityAdd BLAST171
Regioni415 – 524Interaction with MYO6By similarityAdd BLAST110

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili38 – 180Sequence analysisAdd BLAST143
Coiled coili243 – 278Sequence analysisAdd BLAST36
Coiled coili307 – 511Sequence analysisAdd BLAST205

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi186 – 191LIR6
Motifi477 – 482UBAN6

Domaini

Ubiquitin-binding motif (UBAN) is essential for its inhibitory function, subcellular localization and interaction with TBK1.By similarity
The LIR (LC3-interacting region) motif mediates the interaction with ATG8 family proteins.By similarity

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri554 – 584CCHC NOA-typePROSITE-ProRule annotationAdd BLAST31

Keywords - Domaini

Coiled coil, Zinc-finger

Phylogenomic databases

eggNOGiENOG410IE97. Eukaryota.
ENOG410Z0DF. LUCA.
GeneTreeiENSGT00530000063808.
HOGENOMiHOG000231899.
HOVERGENiHBG106481.
InParanoidiQ8K3K8.
KOiK19946.
OMAiLQVTTLF.
OrthoDBiEOG091G0576.
PhylomeDBiQ8K3K8.
TreeFamiTF326608.

Family and domain databases

InterProiView protein in InterPro
IPR032419. CC2-LZ_dom.
IPR021063. NEMO_N.
IPR034735. NEMO_ZF.
IPR032939. Optineurin.
PANTHERiPTHR31553:SF2. PTHR31553:SF2. 1 hit.
PfamiView protein in Pfam
PF16516. CC2-LZ. 1 hit.
PF11577. NEMO. 1 hit.
PROSITEiView protein in PROSITE
PS51801. ZF_CCHC_NOA. 1 hit.

Sequencei

Sequence statusi: Complete.

Q8K3K8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSHQPLSCLT EKGDSPCETP GNGPSNMVHP SLDTFTPEEL LQQMKELLVE
60 70 80 90 100
NHQLKEAMKL NNQAMKGRFE ELSAWTEKQK EERLLFEMQS KEVKERLKAL
110 120 130 140 150
THENERLKEE LGKFKEKSEK PLEDLTGGYR YPRALEEEVE KLKTQVEQEV
160 170 180 190 200
EHLKIQVMRL RAEKADLLGI VSELQLKLNS GGSSEDSFVE IRMTEGETEG
210 220 230 240 250
AMKEMKNCPT PTRTDPISLS NCTEDARSCA EFEELTVSQL LLCLREGNQK
260 270 280 290 300
VERLEVALRE AKERISDFEK KANGHSSTEK QTARRADREK EDKGQESVGS
310 320 330 340 350
EVETLSIQVT SLFKELQEAH TKLSEAELMK KRLQEKCQAL ERKNSATPSE
360 370 380 390 400
LNEKQELVYS NKKLELQVES MRSEIKMEQA KTEEEKSRLA TLQATHNKLL
410 420 430 440 450
QEHNKALKTI EELTKQQAEK VDKMLLQELS EKLELAEQAL ASKQLQMDEM
460 470 480 490 500
KQTLAKQEED LETMAVLRAQ MEVYCSDFHA ERAAREKIHE EKEQLALQLA
510 520 530 540 550
ILLKENNDIE EGGSRQSLME MQCRHGARTS DSDQQTYLFQ RGAEDRSWQH
560 570 580
GQQPRSIPIH SCPKCGEVLP DIDTLQIHVM DCII
Length:584
Mass (Da):67,018
Last modified:October 1, 2002 - v1
Checksum:i4BCF3BD4F71921F0
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY071834 mRNA. Translation: AAL61853.1.
AY340635
, AY340623, AY340624, AY340625, AY340626, AY340627, AY340628, AY340629, AY340630, AY340631, AY340632, AY340633, AY340634 Genomic DNA. Translation: AAQ21118.1.
AL928662 Genomic DNA. Translation: CAM22578.1.
BC061185 mRNA. Translation: AAH61185.1.
CCDSiCCDS15663.1.
RefSeqiNP_862896.1. NM_181848.4.
XP_006497609.1. XM_006497546.1.
XP_006497610.1. XM_006497547.3.
XP_011237289.1. XM_011238987.2.
UniGeneiMm.116942.

Genome annotation databases

EnsembliENSMUST00000027986; ENSMUSP00000027986; ENSMUSG00000026672.
ENSMUST00000114996; ENSMUSP00000110648; ENSMUSG00000026672.
GeneIDi71648.
KEGGimmu:71648.
UCSCiuc008ifm.1. mouse.

Similar proteinsi

Entry informationi

Entry nameiOPTN_MOUSE
AccessioniPrimary (citable) accession number: Q8K3K8
Secondary accession number(s): A2ASP3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 29, 2005
Last sequence update: October 1, 2002
Last modified: November 22, 2017
This is version 113 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot