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Q8K3K8

- OPTN_MOUSE

UniProt

Q8K3K8 - OPTN_MOUSE

Protein

Optineurin

Gene

Optn

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 83 (01 Oct 2014)
      Sequence version 1 (01 Oct 2002)
      Previous versions | rss
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    Functioni

    Plays an important role in the maintenance of the Golgi complex, in membrane trafficking, in exocytosis, through its interaction with myosin VI and Rab8. Links myosin VI to the Golgi complex and plays an important role in Golgi ribbon formation. Negatively regulates the induction of IFNB in response to RNA virus infection. Plays a neuroprotective role in the eye and optic nerve. Probably part of the TNF-alpha signaling pathway that can shift the equilibrium toward induction of cell death. May act by regulating membrane trafficking and cellular morphogenesis via a complex that contains Rab8 and hungtingtin (HD). Mediates the interaction of Rab8 with the probable GTPase-activating protein TBC1D17 during Rab8-mediated endocytic trafficking, such as of transferrin receptor (TFRC/TfR); regulates Rab8 recruitnment to tubules emanating from the endocytic recycling compartment. Autophagy receptor that interacts directly with both the cargo to become degraded and an autophagy modifier of the MAP1 LC3 family; targets ubiquitin-coated bacteria (xenophagy) and appears to function in the same pathway as SQSTM1 and CALCOCO2/NDP52 By similarity.By similarity

    GO - Molecular functioni

    1. protein binding Source: MGI

    GO - Biological processi

    1. cellular protein localization Source: MGI
    2. Golgi ribbon formation Source: UniProtKB
    3. Golgi to plasma membrane protein transport Source: Ensembl
    4. protein targeting to Golgi Source: Ensembl

    Keywords - Biological processi

    Autophagy

    Enzyme and pathway databases

    ReactomeiREACT_196635. Regulation of PLK1 Activity at G2/M Transition.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Optineurin
    Gene namesi
    Name:Optn
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 2

    Organism-specific databases

    MGIiMGI:1918898. Optn.

    Subcellular locationi

    Cytoplasmperinuclear region By similarity. Golgi apparatus By similarity. Golgi apparatustrans-Golgi network By similarity. Cytoplasmic vesicleautophagosome By similarity. Cytoplasmic vesicle By similarity. Recycling endosome By similarity
    Note: Found in the perinuclear region and associates with the Golgi apparatus. Colocalizes with MYO6 and RAB8 at the Golgi complex and in vesicular structures close to the plasma membrane. Localizes to LC3-positive cytoplasmic vesicles upon induction of autophagy By similarity.By similarity

    GO - Cellular componenti

    1. perinuclear region of cytoplasm Source: MGI
    2. trans-Golgi network Source: UniProtKB

    Keywords - Cellular componenti

    Cytoplasm, Cytoplasmic vesicle, Endosome, Golgi apparatus

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi50 – 501E → K: Disrupts interaction with Rab8; accumulation in the retinal outer plexiform layer; loss of retinal ganglion cells and connecting synapses, degeneration of entire retina without elevation of intraocular presssure. 2 Publications

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 584584OptineurinPRO_0000058069Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei187 – 1871Phosphoserine; by TBK1By similarity

    Post-translational modificationi

    Phosphorylated by TBK1, leading to restrict bacterial proliferation in case of infection.By similarity

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiQ8K3K8.
    PaxDbiQ8K3K8.
    PRIDEiQ8K3K8.

    PTM databases

    PhosphoSiteiQ8K3K8.

    Expressioni

    Tissue specificityi

    In eye, it is expressed in anterior segment, retina, and optic nerve blood vessels (at protein level). Highly expressed in adult liver, heart and testis.1 Publication

    Developmental stagei

    Expressed from 7-day-old embryos.1 Publication

    Gene expression databases

    BgeeiQ8K3K8.
    CleanExiMM_OPTN.
    GenevestigatoriQ8K3K8.

    Interactioni

    Subunit structurei

    Interacts with HD, Rab8 (RAB8A and/or RAB8B) (active GTP-bound form), GTF3A, TRAF3, TBK1, MYO6 and TFRC. Binds to linear ubiquitin chains. Interacts with LC3 family members MAP1LC3A, MAP1LC3B, GABARAP, GABARAPL1 and GABARAPL2; OPTN phosphorylation increases the asscociation (at least with MAP1LC3B). Self-associates. Interacts with RAB12; the interaction may be indirect.1 Publication

    Protein-protein interaction databases

    BioGridi214832. 3 interactions.

    Structurei

    3D structure databases

    ProteinModelPortaliQ8K3K8.
    SMRiQ8K3K8. Positions 424-510, 557-584.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni58 – 219162Interaction with Rab8By similarityAdd
    BLAST
    Regioni414 – 584171Interaction with HDBy similarityAdd
    BLAST
    Regioni415 – 524110Interaction with MYO6By similarityAdd
    BLAST

    Coiled coil

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Coiled coili38 – 180143Sequence AnalysisAdd
    BLAST
    Coiled coili243 – 27836Sequence AnalysisAdd
    BLAST
    Coiled coili307 – 511205Sequence AnalysisAdd
    BLAST

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi186 – 1916LIR
    Motifi477 – 4826UBAN

    Domaini

    Ubiquitin-binding motif (UBAN) is essential for its inhibitory function, subcellular localization and interaction with TBK1.By similarity
    The LIR (LC3-interacting region) motif mediates the interaction with ATG8 family proteins.By similarity

    Keywords - Domaini

    Coiled coil

    Phylogenomic databases

    eggNOGiNOG138369.
    GeneTreeiENSGT00530000063808.
    HOGENOMiHOG000231899.
    HOVERGENiHBG106481.
    InParanoidiA2ASP3.
    OMAiAHPNLDT.
    OrthoDBiEOG7D2FD7.
    PhylomeDBiQ8K3K8.
    TreeFamiTF326608.

    Family and domain databases

    InterProiIPR021063. NEMO_N.
    [Graphical view]
    PfamiPF11577. NEMO. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q8K3K8-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSHQPLSCLT EKGDSPCETP GNGPSNMVHP SLDTFTPEEL LQQMKELLVE    50
    NHQLKEAMKL NNQAMKGRFE ELSAWTEKQK EERLLFEMQS KEVKERLKAL 100
    THENERLKEE LGKFKEKSEK PLEDLTGGYR YPRALEEEVE KLKTQVEQEV 150
    EHLKIQVMRL RAEKADLLGI VSELQLKLNS GGSSEDSFVE IRMTEGETEG 200
    AMKEMKNCPT PTRTDPISLS NCTEDARSCA EFEELTVSQL LLCLREGNQK 250
    VERLEVALRE AKERISDFEK KANGHSSTEK QTARRADREK EDKGQESVGS 300
    EVETLSIQVT SLFKELQEAH TKLSEAELMK KRLQEKCQAL ERKNSATPSE 350
    LNEKQELVYS NKKLELQVES MRSEIKMEQA KTEEEKSRLA TLQATHNKLL 400
    QEHNKALKTI EELTKQQAEK VDKMLLQELS EKLELAEQAL ASKQLQMDEM 450
    KQTLAKQEED LETMAVLRAQ MEVYCSDFHA ERAAREKIHE EKEQLALQLA 500
    ILLKENNDIE EGGSRQSLME MQCRHGARTS DSDQQTYLFQ RGAEDRSWQH 550
    GQQPRSIPIH SCPKCGEVLP DIDTLQIHVM DCII 584
    Length:584
    Mass (Da):67,018
    Last modified:October 1, 2002 - v1
    Checksum:i4BCF3BD4F71921F0
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY071834 mRNA. Translation: AAL61853.1.
    AY340635
    , AY340623, AY340624, AY340625, AY340626, AY340627, AY340628, AY340629, AY340630, AY340631, AY340632, AY340633, AY340634 Genomic DNA. Translation: AAQ21118.1.
    AL928662 Genomic DNA. Translation: CAM22578.1.
    BC061185 mRNA. Translation: AAH61185.1.
    CCDSiCCDS15663.1.
    RefSeqiNP_862896.1. NM_181848.4.
    XP_006497609.1. XM_006497546.1.
    XP_006497610.1. XM_006497547.1.
    UniGeneiMm.116942.

    Genome annotation databases

    EnsembliENSMUST00000027986; ENSMUSP00000027986; ENSMUSG00000026672.
    ENSMUST00000114996; ENSMUSP00000110648; ENSMUSG00000026672.
    GeneIDi71648.
    KEGGimmu:71648.
    UCSCiuc008ifm.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY071834 mRNA. Translation: AAL61853.1 .
    AY340635
    , AY340623 , AY340624 , AY340625 , AY340626 , AY340627 , AY340628 , AY340629 , AY340630 , AY340631 , AY340632 , AY340633 , AY340634 Genomic DNA. Translation: AAQ21118.1 .
    AL928662 Genomic DNA. Translation: CAM22578.1 .
    BC061185 mRNA. Translation: AAH61185.1 .
    CCDSi CCDS15663.1.
    RefSeqi NP_862896.1. NM_181848.4.
    XP_006497609.1. XM_006497546.1.
    XP_006497610.1. XM_006497547.1.
    UniGenei Mm.116942.

    3D structure databases

    ProteinModelPortali Q8K3K8.
    SMRi Q8K3K8. Positions 424-510, 557-584.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 214832. 3 interactions.

    PTM databases

    PhosphoSitei Q8K3K8.

    Proteomic databases

    MaxQBi Q8K3K8.
    PaxDbi Q8K3K8.
    PRIDEi Q8K3K8.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000027986 ; ENSMUSP00000027986 ; ENSMUSG00000026672 .
    ENSMUST00000114996 ; ENSMUSP00000110648 ; ENSMUSG00000026672 .
    GeneIDi 71648.
    KEGGi mmu:71648.
    UCSCi uc008ifm.1. mouse.

    Organism-specific databases

    CTDi 10133.
    MGIi MGI:1918898. Optn.

    Phylogenomic databases

    eggNOGi NOG138369.
    GeneTreei ENSGT00530000063808.
    HOGENOMi HOG000231899.
    HOVERGENi HBG106481.
    InParanoidi A2ASP3.
    OMAi AHPNLDT.
    OrthoDBi EOG7D2FD7.
    PhylomeDBi Q8K3K8.
    TreeFami TF326608.

    Enzyme and pathway databases

    Reactomei REACT_196635. Regulation of PLK1 Activity at G2/M Transition.

    Miscellaneous databases

    NextBioi 334153.
    PROi Q8K3K8.
    SOURCEi Search...

    Gene expression databases

    Bgeei Q8K3K8.
    CleanExi MM_OPTN.
    Genevestigatori Q8K3K8.

    Family and domain databases

    InterProi IPR021063. NEMO_N.
    [Graphical view ]
    Pfami PF11577. NEMO. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Molecular cloning, genomic structure, and protein characterization of mouse optineurin."
      Rezaie T., Sarfarazi M.
      Genomics 85:131-138(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], SUBCELLULAR LOCATION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
      Strain: 129.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: C57BL/6J.
    3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Kidney.
    4. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Liver.
    5. "Overexpression of optineurin E50K disrupts Rab8 interaction and leads to a progressive retinal degeneration in mice."
      Chi Z.L., Akahori M., Obazawa M., Minami M., Noda T., Nakaya N., Tomarev S., Kawase K., Yamamoto T., Noda S., Sasaoka M., Shimazaki A., Takada Y., Iwata T.
      Hum. Mol. Genet. 19:2606-2615(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH RAB8, MUTAGENESIS OF GLU-50.
    6. "Enhanced optineurin E50K-TBK1 interaction evokes protein insolubility and initiates familial primary open-angle glaucoma."
      Minegishi Y., Iejima D., Kobayashi H., Chi Z.L., Kawase K., Yamamoto T., Seki T., Yuasa S., Fukuda K., Iwata T.
      Hum. Mol. Genet. 22:3559-3567(2013) [PubMed] [Europe PMC] [Abstract]
      Cited for: MUTAGENESIS OF GLU-50.

    Entry informationi

    Entry nameiOPTN_MOUSE
    AccessioniPrimary (citable) accession number: Q8K3K8
    Secondary accession number(s): A2ASP3
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 29, 2005
    Last sequence update: October 1, 2002
    Last modified: October 1, 2014
    This is version 83 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    OPTN E59K transgenic mice exhibit profound gliosis in the retina.

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

    External Data

    Dasty 3