Reviewed, UniProtKB/Swiss-Prot Q8K3K7 (PLCB_MOUSE)

Names and origin

Protein names

Recommended name:
    1-acyl-sn-glycerol-3-phosphate acyltransferase beta
    EC=2.3.1.51
Alternative name(s):
    1-acylglycerol-3-phosphate O-acyltransferase 2
      Short name=1-AGP acyltransferase 2
      Short name=1-AGPAT 2
    Lysophosphatidic acid acyltransferase beta
      Short name=LPAAT-beta

Gene names

Name:

Agpat2

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	278 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Converts lysophosphatidic acid (LPA) into phosphatidic acid by incorporating an acyl moiety at the sn-2 position of the glycerol backbone.
Catalytic activity	Acyl-CoA + 1-acyl-sn-glycerol 3-phosphate = CoA + 1,2-diacyl-sn-glycerol 3-phosphate.
Pathway	Phospholipid metabolism; CDP-diacylglycerol biosynthesis; CDP-diacylglycerol from sn-glycerol 3-phosphate: step 2/3.
Subcellular location	Membrane; Multi-pass membrane protein Potential.
Domain	The HXXXXD motif is essential for acyltransferase activity and may constitute the binding site for the phosphate moiety of the glycerol-3-phosphate By similarity.
Sequence similarities	Belongs to the 1-acyl-sn-glycerol-3-phosphate acyltransferase family.

Ontologies

Keywords
Biological process	Phospholipid biosynthesis
Cellular component	Membrane
Domain	Transmembrane
Molecular function	Acyltransferase Transferase
PTM	Phosphoprotein
Gene Ontology (GO)
Biological process	phospholipid biosynthetic process Non-traceable author statement. Source: UniProtKB
Cellular component	endoplasmic reticulum Inferred from direct assay. Source: UniProtKB integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	1-acylglycerol-3-phosphate O-acyltransferase activity Inferred from direct assay. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 278	278	1-acyl-sn-glycerol-3-phosphate acyltransferase beta		PRO_0000208193
Regions
	Transmembrane	1 – 21	21	Potential
	Transmembrane	30 – 50	21	Potential
	Transmembrane	122 – 142	21	Potential
	Motif	98 – 103	6	HXXXXD motif
Amino acid modifications
	Modified residue	255	1	Phosphothreonine Ref.2
	Modified residue	260	1	Phosphoserine Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Q8K3K7-1 [UniParc]. Last modified October 1, 2002. Version 1. Checksum: 57A54BFB1A0EFF56	FASTA	278	31,011
	10 20 30 40 50 60 MDPWPWLTAA LLLLLLLVQL SRTARFYAKV GLYCVLCLSF SAAASIVCLL RHGGRTVDNM 70 80 90 100 110 120 SIISWFVRSF KYVYGLRFEV SGQKKLEVDG PCVIISNHQS ILDMMGLMEI LPKRCVQIAK 130 140 150 160 170 180 RELMFTGPVG LIMYLGGVYF INRQQARTAM SVMADLGDLM VKENLKVWIY PEGTRNDNGD 190 200 210 220 230 240 LLPFKKGAFY LAIQAQVPII PVVYSSFSSF YNVKTKLFTS GTIKVQVLDA VPTNGLTDAD 250 260 270 VTKLVDTCYQ SMRATFLQIS QIPQENSAIK EPGVLPAQ

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"AGPAT2 is mutated in congenital generalized lipodystrophy linked to chromosome 9q34." Agarwal A.K., Arioglu E., de Almeida S., Akkoc N., Taylor S.I., Bowcock A.M., Barnes R.I., Garg A. Nat. Genet. 31:21-23(2002) [PubMed: 11967537] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: BALB/c. Tissue: Liver.
[2]	"Protein phosphorylation and expression profiling by Yin-yang multidimensional liquid chromatography (Yin-yang MDLC) mass spectrometry." Dai J., Jin W.-H., Sheng Q.-H., Shieh C.-H., Wu J.-R., Zeng R. J. Proteome Res. 6:250-262(2007) [PubMed: 17203969] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-255 AND SER-260, MASS SPECTROMETRY. Tissue: Liver.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AY072769 mRNA. Translation: AAL62337.1.
IPI	IPI00170100.
RefSeq	NP_080488.1.
UniGene	Mm.24244
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q8K3K7.
PTM databases
PhosphoSite	Q8K3K7.
Proteomic databases
PRIDE	Q8K3K7.
Genome annotation databases
Ensembl	ENSMUST00000028286; ENSMUSP00000028286; ENSMUSG00000026922; Mus musculus. [Genome view]
GeneID	67512.
KEGG	mmu:67512.
UCSC	uc008ivt.1. mouse.
Organism-specific databases
CTD	67512.
MGI	MGI:1914762. Agpat2.
Phylogenomic databases
HOGENOM	Q8K3K7.
HOVERGEN	Q8K3K7.
OMA	EGTRNDN.
Enzyme and pathway databases
BRENDA	2.3.1.51. 244.
Gene expression databases
ArrayExpress	Q8K3K7.
Bgee	Q8K3K7.
Genevestigator	Q8K3K7.
GermOnline	ENSMUSG00000026922. Mus musculus.
Family and domain databases
InterPro	IPR002123. Acyltransferase. IPR004552. AGP_acyltrans. [Graphical view]
Pfam	PF01553. Acyltransferase. 1 hit. [Graphical view]
SMART	SM00563. PlsC. 1 hit. [Graphical view]
TIGRFAMs	TIGR00530. AGP_acyltrn. 1 hit.
ProtoNet	Search...
Other Resources
NextBio	324790.
SOURCE	Search...

Entry information

Entry name

PLCB_MOUSE

Accession

Primary (citable) accession number: Q8K3K7

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 21, 2005
Last sequence update:	October 1, 2002
Last modified:	November 3, 2009
This is version 50 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)