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Protein

1-acyl-sn-glycerol-3-phosphate acyltransferase beta

Gene

Agpat2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Converts lysophosphatidic acid (LPA) into phosphatidic acid by incorporating an acyl moiety at the sn-2 position of the glycerol backbone.1 Publication

Catalytic activityi

Acyl-CoA + 1-acyl-sn-glycerol 3-phosphate = CoA + 1,2-diacyl-sn-glycerol 3-phosphate.

Pathwayi: CDP-diacylglycerol biosynthesis

This protein is involved in step 2 of the subpathway that synthesizes CDP-diacylglycerol from sn-glycerol 3-phosphate.
Proteins known to be involved in the 3 steps of the subpathway in this organism are:
  1. Glycerol-3-phosphate acyltransferase 1, mitochondrial (Gpam), Glycerol-3-phosphate acyltransferase 4 (Gpat4), Glycerol-3-phosphate acyltransferase 3 (Gpat3), Glycerol-3-phosphate acyltransferase 2, mitochondrial (Gpat2)
  2. 1-acyl-sn-glycerol-3-phosphate acyltransferase gamma (Agpat3), 1-acyl-sn-glycerol-3-phosphate acyltransferase beta (Agpat2), 1-acyl-sn-glycerol-3-phosphate acyltransferase alpha (Agpat1), Lysocardiolipin acyltransferase 1 (Lclat1), 1-acyl-sn-glycerol-3-phosphate acyltransferase epsilon (Agpat5), 1-acyl-sn-glycerol-3-phosphate acyltransferase delta (Agpat4)
  3. Phosphatidate cytidylyltransferase, mitochondrial (Tamm41), Phosphatidate cytidylyltransferase, mitochondrial (Tamm41), Phosphatidate cytidylyltransferase (Cds2), Phosphatidate cytidylyltransferase (Cds2), Phosphatidate cytidylyltransferase 2 (Cds2), Phosphatidate cytidylyltransferase (Cds2), Phosphatidate cytidylyltransferase 1 (Cds1)
This subpathway is part of the pathway CDP-diacylglycerol biosynthesis, which is itself part of Phospholipid metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes CDP-diacylglycerol from sn-glycerol 3-phosphate, the pathway CDP-diacylglycerol biosynthesis and in Phospholipid metabolism.

GO - Molecular functioni

  • 1-acylglycerol-3-phosphate O-acyltransferase activity Source: UniProtKB

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Acyltransferase, Transferase

Keywords - Biological processi

Lipid biosynthesis, Lipid metabolism, Phospholipid biosynthesis, Phospholipid metabolism

Enzyme and pathway databases

ReactomeiR-MMU-1483166. Synthesis of PA.
R-MMU-75109. Triglyceride Biosynthesis.
UniPathwayiUPA00557; UER00613.

Names & Taxonomyi

Protein namesi
Recommended name:
1-acyl-sn-glycerol-3-phosphate acyltransferase beta (EC:2.3.1.51)
Alternative name(s):
1-acylglycerol-3-phosphate O-acyltransferase 2
Short name:
1-AGP acyltransferase 2
Short name:
1-AGPAT 2
Lysophosphatidic acid acyltransferase beta
Short name:
LPAAT-beta
Gene namesi
Name:Agpat2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 2

Organism-specific databases

MGIiMGI:1914762. Agpat2.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei30 – 5021HelicalSequence analysisAdd
BLAST
Transmembranei122 – 14221HelicalSequence analysisAdd
BLAST
Transmembranei187 – 20721HelicalSequence analysisAdd
BLAST

GO - Cellular componenti

  • endoplasmic reticulum Source: UniProtKB
  • endoplasmic reticulum membrane Source: UniProtKB-SubCell
  • integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2323Sequence analysisAdd
BLAST
Chaini24 – 2782551-acyl-sn-glycerol-3-phosphate acyltransferase betaPRO_0000208193Add
BLAST

Proteomic databases

MaxQBiQ8K3K7.
PaxDbiQ8K3K7.
PRIDEiQ8K3K7.

PTM databases

iPTMnetiQ8K3K7.
PhosphoSiteiQ8K3K7.

Expressioni

Tissue specificityi

Expressed at high levels in the liver, at intermediate levels in the kidney, gut, heart and skeletal muscles. Undetectable in brain and spleen.1 Publication

Inductioni

Down-regulated in the heart by clofibrate, a PPAR-alpha agonist.1 Publication

Gene expression databases

BgeeiENSMUSG00000026922.
ExpressionAtlasiQ8K3K7. baseline and differential.
GenevisibleiQ8K3K7. MM.

Interactioni

Protein-protein interaction databases

MINTiMINT-1858721.
STRINGi10090.ENSMUSP00000028286.

Structurei

3D structure databases

ProteinModelPortaliQ8K3K7.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi98 – 1036HXXXXD motif
Motifi172 – 1754EGTR motif

Domaini

The HXXXXD motif is essential for acyltransferase activity and may constitute the binding site for the phosphate moiety of the glycerol-3-phosphate.By similarity

Sequence similaritiesi

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG2848. Eukaryota.
COG0204. LUCA.
GeneTreeiENSGT00390000008726.
HOGENOMiHOG000026375.
HOVERGENiHBG000676.
InParanoidiQ8K3K7.
KOiK13509.
OMAiMSIISWF.
OrthoDBiEOG091G0ICC.
PhylomeDBiQ8K3K7.
TreeFamiTF314867.

Family and domain databases

InterProiIPR004552. AGP_acyltrans.
IPR002123. Plipid/glycerol_acylTrfase.
[Graphical view]
PfamiPF01553. Acyltransferase. 1 hit.
[Graphical view]
SMARTiSM00563. PlsC. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00530. AGP_acyltrn. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q8K3K7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDPWPWLTAA LLLLLLLVQL SRTARFYAKV GLYCVLCLSF SAAASIVCLL
60 70 80 90 100
RHGGRTVDNM SIISWFVRSF KYVYGLRFEV SGQKKLEVDG PCVIISNHQS
110 120 130 140 150
ILDMMGLMEI LPKRCVQIAK RELMFTGPVG LIMYLGGVYF INRQQARTAM
160 170 180 190 200
SVMADLGDLM VKENLKVWIY PEGTRNDNGD LLPFKKGAFY LAIQAQVPII
210 220 230 240 250
PVVYSSFSSF YNVKTKLFTS GTIKVQVLDA VPTNGLTDAD VTKLVDTCYQ
260 270
SMRATFLQIS QIPQENSAIK EPGVLPAQ
Length:278
Mass (Da):31,011
Last modified:October 1, 2002 - v1
Checksum:i57A54BFB1A0EFF56
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY072769 mRNA. Translation: AAL62337.1.
CCDSiCCDS15809.1.
RefSeqiNP_080488.1. NM_026212.2.
UniGeneiMm.24244.

Genome annotation databases

EnsembliENSMUST00000028286; ENSMUSP00000028286; ENSMUSG00000026922.
GeneIDi67512.
KEGGimmu:67512.
UCSCiuc008ivt.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY072769 mRNA. Translation: AAL62337.1.
CCDSiCCDS15809.1.
RefSeqiNP_080488.1. NM_026212.2.
UniGeneiMm.24244.

3D structure databases

ProteinModelPortaliQ8K3K7.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

MINTiMINT-1858721.
STRINGi10090.ENSMUSP00000028286.

PTM databases

iPTMnetiQ8K3K7.
PhosphoSiteiQ8K3K7.

Proteomic databases

MaxQBiQ8K3K7.
PaxDbiQ8K3K7.
PRIDEiQ8K3K7.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000028286; ENSMUSP00000028286; ENSMUSG00000026922.
GeneIDi67512.
KEGGimmu:67512.
UCSCiuc008ivt.2. mouse.

Organism-specific databases

CTDi10555.
MGIiMGI:1914762. Agpat2.

Phylogenomic databases

eggNOGiKOG2848. Eukaryota.
COG0204. LUCA.
GeneTreeiENSGT00390000008726.
HOGENOMiHOG000026375.
HOVERGENiHBG000676.
InParanoidiQ8K3K7.
KOiK13509.
OMAiMSIISWF.
OrthoDBiEOG091G0ICC.
PhylomeDBiQ8K3K7.
TreeFamiTF314867.

Enzyme and pathway databases

UniPathwayiUPA00557; UER00613.
ReactomeiR-MMU-1483166. Synthesis of PA.
R-MMU-75109. Triglyceride Biosynthesis.

Miscellaneous databases

PROiQ8K3K7.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000026922.
ExpressionAtlasiQ8K3K7. baseline and differential.
GenevisibleiQ8K3K7. MM.

Family and domain databases

InterProiIPR004552. AGP_acyltrans.
IPR002123. Plipid/glycerol_acylTrfase.
[Graphical view]
PfamiPF01553. Acyltransferase. 1 hit.
[Graphical view]
SMARTiSM00563. PlsC. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00530. AGP_acyltrn. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiPLCB_MOUSE
AccessioniPrimary (citable) accession number: Q8K3K7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 21, 2005
Last sequence update: October 1, 2002
Last modified: September 7, 2016
This is version 107 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.