Q8K3K4 (SPB10_RAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Serpin B10

Alternative name(s):
TGF-beta-repressible serine proteinase inhibitor
Short name=Trespin
Transforming growth factor beta repressible serpin

Gene names

Name:

Serpinb10

Organism

Rattus norvegicus (Rat) [Reference proteome]

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	397 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Protease inhibitor that may play a role in the regulation of protease activities during hematopoiesis and apoptosis induced by TNF. May regulate protease activities in the cytoplasm and in the nucleus By similarity. Inhibits plasmin. Ref.1
Subcellular location	Nucleus By similarity. Cytoplasm Ref.1.
Tissue specificity	Expressed in many tissues, including brain, heart, kidney, liver, lung, prostate, skin, spleen and stomach. Ref.1
Sequence similarities	Belongs to the serpin family. Ov-serpin subfamily.

Ontologies

Keywords
Cellular component	Cytoplasm Nucleus
Molecular function	Protease inhibitor Serine protease inhibitor
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	negative regulation of proteolysis Inferred from direct assay Ref.1. Source: RGD
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	serine-type endopeptidase inhibitor activity Inferred from Biological aspect of Ancestor. Source: RefGenome
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 397	397	Serpin B10		PRO_0000094116
Regions
	Motif	74 – 77	4	Nuclear localization signal By similarity
Sites
	Site	362 – 363	2	Reactive bond By similarity
Experimental info
	Sequence conflict	88	1	M → I in AAH61735. Ref.2
	Sequence conflict	164	1	D → G in AAH61735. Ref.2
	Sequence conflict	288	1	R → Q in AAH61735. Ref.2
	Sequence conflict	391	1	D → Y in AAH61735. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Q8K3K4 [UniParc]. Last modified October 1, 2002. Version 1. Checksum: 19E7BF371A9D2F4C	FASTA	397	45,387
	10 20 30 40 50 60 MASLAVSINQ FAVEFSKKLA ESAEGRNIFF SPWGISTSLA MVYLGTKGTT AAQMSQVLHF 70 80 90 100 110 120 GSIQDFKFGP DSEKKRKMEC HSGKSEEMQS DFQTLTAKIL KHGNSYVLKI ANRIYVEKTY 130 140 150 160 170 180 LFHNKYLEDM KTYFGAEPQS VNFVEASGQI RKEINSWVGS QTGDKIPNLL PDDAVDNKTT 190 200 210 220 230 240 MVLVNALYFK GTWEHQFSVQ NTTERPFRIN KTTSKPVQMM SMKQSLQVFH IEELQTIGVQ 250 260 270 280 290 300 LHYQNREFSL LLLLPEEVEG LKQLERAITY EKLDKWTSAD MMDTYEVRLY LPKFKMEESY 310 320 330 340 350 360 DLQSALRDMG MTDAFNQGKA NFSNMTSERN LFLSNVFHKT FLEINEEGTE AAAGTGSEVN 370 380 390 FRIKAPSIEL NADHPFLFLI RHNVTNTILF DGRFYSP

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Identification and characterization of a novel rat ov-serpin family member, trespin." Chipuk J.E., Stewart L.V., Ranieri A., Song K., Danielpour D. J. Biol. Chem. 277:26412-26421(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY. Strain: Wistar.
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Prostate.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AY075037 mRNA. Translation: AAL78042.1. BC061735 mRNA. Translation: AAH61735.1.
IPI	IPI00470282.
RefSeq	NP_714955.2. NM_153733.2.
UniGene	Rn.59484.
3D structure databases
ProteinModelPortal	Q8K3K4.
ModBase	Search...
Protein family/group databases
MEROPS	I04.015.
Proteomic databases
PRIDE	Q8K3K4.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	266775.
KEGG	rno:266775.
UCSC	RGD:628853. rat.
Organism-specific databases
CTD	5273.
RGD	628853. Serpinb10.
Phylogenomic databases
eggNOG	COG4826.
HOGENOM	HOG000238519.
HOVERGEN	HBG005957.
InParanoid	Q8K3K4.
KO	K13963.
OrthoDB	EOG4DR9CH.
Gene expression databases
Genevestigator	Q8K3K4.
GermOnline	ENSRNOG00000002417. Rattus norvegicus.
Family and domain databases
InterPro	IPR023795. Protease_inhib_I4_serpin_CS. IPR023796. Serpin_dom. IPR000215. Serpin_fam. [Graphical view]
PANTHER	PTHR11461. PTHR11461. 1 hit.
Pfam	PF00079. Serpin. 1 hit. [Graphical view]
SMART	SM00093. SERPIN. 1 hit. [Graphical view]
SUPFAM	SSF56574. Prot_inh_serpin. 1 hit.
PROSITE	PS00284. SERPIN. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	624656.

Entry information

Entry name

SPB10_RAT

Accession

Primary (citable) accession number: Q8K3K4
Secondary accession number(s): Q6P7C1

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 5, 2005
Last sequence update:	October 1, 2002
Last modified:	April 3, 2013
This is version 68 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program